A Synthetic Naringenin Derivative, 5-Hydroxy-7,4′-diacetyloxyflavanone-<i>N</i>-phenyl Hydrazone (N101-43), Induces Apoptosis through Up-regulation of Fas/FasL Expression and Inhibition of PI3K/Akt Signaling Pathways in Non-Small-Cell Lung Cancer Cells

Bak, Yesol,Kim, Heejong,Kang, Jeong-Woo,Lee, Dong Hun,Kim, Man Sub,Park, Yun Sun,Kim, Jung-Hee,Jung, Kang-Yeoun,Lim, Yoongho,Hong, Jintae,Yoon, Do-Young American Chemical Society 2011 Journal of agricultural and food chemistry Vol.59 No.18

<P>Naringenin, a well-known naturally occurring flavonone, demonstrates cytotoxicity in a variety of human cancer cell lines; its inhibitory effects on tumor growth have spurred interest in its therapeutic application. In this study, naringenin was derivatized to produce more effective small-molecule inhibitors of cancer cell proliferation, and the anticancer effects of its derivative, 5-hydroxy-7,4′-diacetyloxyflavanone-<I>N</I>-phenyl hydrazone (N101-43), in non-small-cell lung cancer (NSCLC) cell lines NCI-H460, A549, and NCI-H1299 were investigated. Naringenin itself possesses no cytotoxicity against lung cancer cells. In contrast, N101-43 inhibits proliferation of both NCI-H460 and A549 cell lines; this capacity is lost in p53-lacking NCI-H1299 cells. N101-43 induces apoptosis via sub-G<SUB>1</SUB> cell-cycle arrest in NCI-H460 and via G<SUB>0</SUB>/G<SUB>1</SUB> arrest in A549 cells. Expression of apoptosis and cell-cycle regulatory factors is altered: Cyclins A and D1 and phospho-pRb are down-regulated, but expression of CDK inhibitors such as p21, p27, and p53 is enhanced by N101-43 treatment; N101-43 also increases expression levels of the extrinsic death receptor Fas and its binding partner FasL. Furthermore, N101-43 treatment diminishes levels of cell survival factors such as PI3K and p-Akt dose-dependently, and N101-43 additionally induces cleavage of the pro-apoptotic factors caspase-3, caspase-8, and poly ADP-ribose polymerase (PARP). Cumulatively, these investigations show that the naringenin derivative N101-43 induces apoptosis via up-regulation of Fas/FasL expression, activation of caspase cascades, and inhibition of PI3K/Akt survival signaling pathways in NCI-H460 and A549 cells. In conclusion, these data indicate that N101-43 may have potential as an anticancer agent in NSCLC.</P>

[논문]보양조건 및 타설주기를 고려한 매스 콘크리트 구조물의 수화열 해석

이영호,서기영,최덕락,엄장섭,진치섭* 釜山大學校生産技術硏究所 2004 生産技術硏究所論文集 Vol.63 No.-

콘크리트 구조물의 공용수명은 콘크리트 재료의 초기에 발생하는 수화열에 의해 아주 큰 영향을 받는다. 수화열은 콘크리트의 열전달 및 열응력 특성으로 결정되어지는 온도균열지수를 통해 분석되고 있다. 본 연구에서는 이러한 온도균열 지수의 분석을 통해 매스 콘크리트 구조물의 시공시 적절한 시벤트 종류, 보양방법,타설주기 및 타설높이 둥 최적의 시공방안 구하는데 도움을 주어 향후 시공 예정인 매스 콘크리트 구조물에 발생 가능한 온도균열을 미리 예측하여 이를 방지하기 위한 시공 방안을 제안하는데 그 목적을 두고 있다.

대기행렬 네트워크상에서 협업이 이루어지지 않는 두 서비스 센터의 선정 방안

소영섭,허은영 전북대학교 공업기술연구소 1995 工學硏究 Vol.26 No.-

The paper considers the location-allocation algorithm of two facility on queueing networks. In the case of multi-facility, the problem is to find simultaneously the optomal districting policy and the optimal locations of facilities. Berman & mondowsky[4] presented the heuristic method of location-allocation on congested networks in the case of multi-facility. But whenever the locations of faciltiess change, Berman & Mondiwsky's algorithm has to find the districting policy using MIP(Mixed Integer Program). Consequently, the computational time increases. Hence, we suggest the new heuristic algorithm to decrease the computational time. We equalize the queueing delay of two sub-networks using the difference between the service times of each nodes form the two facilities and change allocation of only one node at each pivot. The experience results show that the objective value of our algorithm is as good as Berman & Monowsky's and the computational time is smaller than Berman & Mondowsky's

Shelf Life Determination of Precooked Frozen Meat Dumplings

Lee, Young-Chun,Yang, Hyung-Sub,Kim, Dong-Ho 한국산업식품공학회 2002 산업 식품공학 Vol.6 No.3

Quality of frozen foods is significantly affected by the storage temperature and tie, and the consumers' demand for safe quality frozen foods is increasing. The objective of this research was to estimate the shelf life of frozen cooked meat dumplings, based on the changes in the selected physico-chemical and sensory quality attributes. Precooked frozen meat dumplings sampled from the same lot produced at a frozen food company in Seoul were stored at -10, -15, and -20℃. The meat dumplings sampled at 3- to 4-week intervals were subjected to the determination of volatile basic nitrogen (VBN), thiobarbituric acid (TBA), peroxide value (POV), surface color and shear value after thawing at 5℃ for 15 h. High quality life (HQL) was determined through the triangle test and practical quality life (PQL) through the scoring test. VBN, TBA and POV increased as the storage period was increased. HQL of the frozen meat dumplings were 189 and 252 days at -10 and -15℃, respectively. The estimated PQL were 349, 475, and 491 days at -10, -15, and -20 ℃, respectively. Statistical analysis indicated that there were significant relationships between sensory scores and VBN and POV of the stored meat dumplings. VBN was selected as the most reliable objective quality index for the shelf life determination (PQL) of the precooked frozen meat dumplings.

老化過程에서 흰쥐 滑車神經의 軸索의 數 및 直徑의 變化

최영수,엄창섭,서영석 최신의학사 1987 最新醫學 Vol.30 No.8

Al - Ferrite의 Mossbauer 분광학적 연구

이충섭(Choong-Sub Lee),주한식(Han-Sik Joo),이찬영(Chan-Young Lee),서정철(Jung-Chul Sur) 한국자기학회 1998 韓國磁氣學會誌 Vol.8 No.4

We have studied Al_xFe_(3-x)O₄ produced by direct composition method using X-ray diffraction and Mssbauer spectroscopy. The cation distribution for Al_xFe_(3-x)O₄ was determined by the ratio of sub-spectra absorption area. The charge state of Fe atoms in octahedral site(B-site) is Fe^(2.5+) based on electron hopping, Fe²+ ↔ (Fe³+, Al³+) without dependency of substituted Al amounts.

초등학생용 진로성숙도 검사 개발 및 타당화

송인섭 ( In Sub Song ),정미경 ( Mi Kyung Chung ),김효원 ( Hyo Won Kim ),최영미 ( Young Mi Choi ) 한국아동교육학회 2011 아동교육 Vol.20 No.3

본 연구는 초등학교 고학년을 대상으로 발달계에서 주어지는 진로문제들에 대해 대처할 수 있는 준비도인 진로성숙도를 측정할 수 있는 검사를 개발하고 타당화하는 데 그 목적이 있다. 이를 위해 먼저, 국내·와 진로성숙도에 대한 각종 논문과 이론을 참고하여 진로성숙도를 개념화하고 관련 검사 문항들을 검토하여 문항을 개발하였다. 예비검사와 타당화 과정을 거쳐 총 78문항을 선정하고 요인분석과 문항난이도, 문항변별도 등을 구하여 검사를 타당화하였다. 그 결과 태도검사의 문항들이 .30 이상의 단일요인 부하량을 나타내어 각 척도가 단일성 요인임을 설명해주고 있으며 능력검사의 신뢰도는 Cronbach α계수 .52~.86으로 나타났다. 계획성 요인의 경우 .52로 일반적인 신뢰도 기준에는 낮은 신뢰도로 판단되나 진위형 척도의 특성상 신뢰도가 낮게 추정된 것으로 보인다. 그 이외의 하위용인에서는 적절한 신뢰도를 나타내었다. 그리고 진로성숙도의 내적 구조를 분석한 결과 진로성숙도의 구조는 진로에 대한 능력과 태도로 구성되며, 태도는 결정성, 독립성, 계획성으로, 능력은 직업이해. 직업선택, 그리고 직업성으로 구성된 모형이 적합함을 알 수 있었다. 본 연구는 지로성숙도를 측정하기 위해 Crites의 CMI모형을 근간으로 측정요인을 선정하고 초등학생에 맞는 연구모형을 재정립하였다는 점에서 이론적 의의가 있으며, 연구의 특성에 적합한 접근 방법으로 도구를 타당화하였다는 점에서 방법론적 의의가 있다. 그리고 아동이 진로를 선택하는 데 있어서 어떠한 정의적 태도와 지적 사고를 가지고 있는가에 대한 정보를 제공함으로써 자신에 대한 이해와 직업에 대한 이해를 바탕으로 올바른 진로선택을 할 수 있도록 도울 수 있으며 진로교육과 관련된 프로그램을 개발하는 데 기초자료로 활용될 수 있다는 점에서 실제적인 의의가 있다. The purpose of this study is 1) to define the concept of the career maturity: 2) to develop and validate the scale of career maturity for elementary school students. In order to answer these purposes, we first reviewed related documents and inventories, as to research the constructed factors which to develop an career maturity scale, then defined the concept of career maturity and the theological constructive model. The were included in 2 components of attitude and cognitive abilities for career maturity accordingly in 6 sub-components 195 elementary school students were analysed at pilot test and 1010 students were conducted final test. Based on factor analysis and item analysis, 77 items were selected to final test items. And this scale was validated through the evidence based on internal structure. Significance, limitations, and future research directions were discussed.

조리종사자의 식자재구매시스템 인지와 비재무적 성과의 영향 관계

정욱영(Wook Young Jeong),오영섭(Young Sub Oh) 한국조리학회 2012 한국조리학회지 Vol.18 No.3

This study aims to analyze how the perception of a food purchasing system affects non-financial performance and job satisfaction among the kitchen employees of contract foodservice companies in Taegu·Gyeongbuk area. By a convenience method, 197 copies(85%) out of 230 copies of the questionnaire were used for analysis. For data processing, the SPSS WIN Version 14.0 statistical package program was used and the frequency analysis reliability analysis, factor analysis were conducted so the hypothesis was verified through the multiple regression analysis. The results are as follows: First, the perception of a food purchasing system affected job satisfaction as a whole, which was significant in food ingredients management factors of sub-hypothesis1-2. Second, the perception of a food purchasing system affected non-financial management performance, which was significant in food ingredients management factors of sub-hypothesis2-2, and food ingredients supply and demand factors of sub-hypothesis2-3. Third, job satisfaction affected non-financial management performance of a company as suggested by hypothesis 3. From these results, it was found that kitchen employees` perception of a food purchasing system can induce their positive attitude, which exerts positive effects on business performance.

Acute and Sub-chronic Oral Toxicity Study of Ammonium Persulfate in Spraque-Dawley Rats

Yong Soon Kim,Min Won Baek,Jae Hyuck Sung,Hyun Youl Ryu,Jin Sik Kim,Hyun Sun Cho,Byung Gil Choi,Min Sub Song,Moon Yong Song,Eun Ju Baik,Young Kuk Choi,Jong Kyu Kim,Il Je Yu,Kyung Seuk Song 한국독성학회 2009 Toxicological Research Vol.25 No.3

The toxicity test of ammonium persulfate was conducted to ensure of its potential toxic effects according to the single-dose acute oral toxicity study (OECD Guideline 423) and 90-day repeated dose sub-chronic oral toxicity study guideline (OECD Guideline 408) for establishing national chemical management system, and matching in the Globally Harmonized Classification System (GHS) category. In acute oral toxicity study, pasty stool, perineal contamination and temporary body weight decrease were observed after dosing 1st and 2nd challenge (300 ㎎/㎏ body weight). All test animals were dead within 6 hours after dosing at 3rd challenge (2000 ㎎/㎏ body weight). Therefore, the GHS class of test substance is considered class 4. In sub-chronic toxicity study, body weight changes, food consumptions, hematological, biochemical and pathological examination did not show any noticeable and significant differences between the administered (5, 20, 80 ㎎/㎏ body weight) and control (vehicle only) group animals. Based on these results, the no observed adverse effect level (NOAEL) is considered above 80 mg/kg body weight.

2,4-bis (p-hydroxyphenyl)-2-butenal (HPB242) induces apoptosis via modulating E7 expression and inhibition of PI3K/Akt pathway in SiHa human cervical cancer cells.

Kim, Man Sub,Kim, Jung Hee,Bak, Yesol,Park, Yun Sun,Lee, Dong Hun,Kang, Jeong Woo,Shim, Jung-Hyun,Jeong, Heon Sang,Hong, Jin Tae,Yoon, Do Young Lawrence Erlbaum Associates, Publishers [etc.] 2012 Nutrition and cancer Vol.64 No.8

<P>The Maillard reaction is a chemical reaction occurring between an amino acid and a reducing sugar, usually requiring thermal processing. Maillard reaction products (MRPs) have antioxidant, antimutagenic, and antibacterial effects, and although 2,4-bis (p-hydroxyphenyl)-2-butenal (HPB242), a fructose-tyrosine MRP, appears to inhibit proliferation of cancer cells, its mechanism of action has not been studied in detail. We found that HPB242 treatment modulated expression of cyclins and tumor suppressor genes in SiHa human cervical cancer cell lines: cyclins and phospho-pRB were downregulated, whereas the expression of CDK inhibitors and p53 was enhanced. HPB242 induced apoptosis dose-dependently by suppressing E7 expression and leading to sub-G1 cell-cycle arrest in SiHa cell lines; treatment also led to the proteolytic cleavage of caspase-3, -9, and poly (ADP-ribose) polymerase. Moreover, HPB242 upregulated Fas expression, altered expressions of pro- and antiapoptotic factors, and also inhibited nuclear translocation of nuclear factor κB and phosphorylation of IκB. HPB242 treatment decreased phosphatidyl inositol-3 kinase and p-Akt expression levels, demonstrating that this survival pathway may also be inhibited by HPB242. Cumulatively, HPB242 promotes apoptosis by influencing E7 expression, inducing cell-cycle arrest at sub-G1 phase, and promoting both intrinsic (mitochondrial) and extrinsic (Fas-dependent) apoptosis in SiHa human cervical cancer cells.</P>