Enhancing the Enzymatic Activity of the Multifunctional β-Glycosyl Hydrolase (Cel44C-Man26A_P558) from Paenibacillus polymyxa GS01 Using DNA Shuffling

강영민,강태호,윤한대,조계만,Kang, Young-Min,Kang, Tae-Ho,Yun, Han-Dae,Cho, Kye-Man The Microbiological Society of Korea 2012 미생물학회지 Vol.48 No.2

본 연구자들은 이전에 cellulase, xyalnase 및 lichenase의 다기능 효소활성을 지니는 절단된 Cel44C-$Man26A_{P558}$의 ${\beta}$-glycosyl hydrolase를 보고하였다. 본 연구에서는 절단된 Cel44C-$Man26A_{P558}$ 효소의 다기능성 ${\beta}$-glycosyl hydrolase 활성을 증가시키기 위해 DNA shuffling을 시도하였다. DNA shuffling에 의해 단일변이(P438A)를 가진 M2Cel44C-$Man26A_{P558}$와 이중변이(A273T 및 P438A)를 가진 M21Cel44C-$Man26A_{P558}$를 얻었다. 이중변이를 가진 M21Cel44C-$Man26A_{P558}$은 단일변이를 가진 M2Cel44C-$Man26A_{P558}$ 보다 효소활성이 낮게 나타났으나, M2Cel44C-$Man26A_{P558}$와 M21Cel44C-$Man26A_{P558}$은 대조구인 Cel44C-$Man26A_{P558}$ 보다 약 1.3에서 2.2배 정도 높은 효소활성을 나타내었다. 특히, 단일변이를 가진 M2Cel44C-$Man26A_{P558}$는 대조구인 Cel44C-$Man26A_{P558}$보다 cellulase, xylanase 및 lichenase 효소활성이 약 1.5에서 2.2배 정도 높게 나타났다. ${\beta}$-Glycosyl hydrolase의 cellulase, linchenase 및 xylanase 최적 효소활성은 각각 pH 7.0, 7.0 및 6.0에서 이었다. 이러한 결과는, 아미노산 잔기인 Ala438이 다기능성 ${\beta}$-glycosyl hydrolase 활성을 증가시키는 중요한 역할을 한다고 추정할 수 있다. We previously reported that the truncated Cel44C-$Man26A_{P558}$ ${\beta}$-glycosyl hydrolase protein exhibits multifunctional activities, including cellulase, xylanase, and lichenase. DNA shuffling of the truncated Cel44C-$Man26A_{P558}$ enzyme was performed to enhance the enzymatic activity of the multifunctional ${\beta}$-glycosyl hydrolase. Two mutant enzymes, M2Cel44C-$Man26A_{P558}$ that carries one mutation (P438A) and M21Cel44C-$Man26A_{P558}$ that carries two mutations (A273T and P438A) were obtained. The enzymatic activity of the M21Cel44C-$Man26A_{P558}$ double mutant was lower than enzymatic activity of the single mutant (M2Cel44C-$Man26A_{P558}$). However, both mutants displayed the enhancements in their enzyme activities that were ${\approx}1.3$- to 2.2-fold higher than the original enzymatic activity in Cel44C-$Man26A_{P558}$. In particular, the mutant M2Cel44C-$Man26A_{P558}$ exhibited an approximate 1.5- to 2.2-fold increase in the cellulase, xylanase, and lichenase activities in comparison with the control (Cel44C-$Man26A_{P558}$). The optimum cellulase, linchenase, and xylanase activities of ${\beta}$-glycosyl hydrolase were observed at pH 7.0, pH 7.0 and pH 6.0, respectively. These results, therefore, suggest that the amino acid residue Ala438 plays important roles in the enhancement of the activity of multifunctional ${\beta}$-glycosyl hydrolase.

위암의 연령에 따른 차이

김영재(Young Jae Kim),이영철(Young Chul Rhee),이만길(man Kill Lee),차호(Ho Cha),정영채(Young Chae Jung) 대한소화기학회 1987 대한소화기학회지 Vol.19 No.1

N/A Comparative analytic review on clinical, endoscopic and histopathological findings was done in 13 cases of young age (under 29), 446 cases of mid age (30~69) and 39 cases of old age (above 70) stomach cancer who were visited Department of Internal Medicine of Masan Koryo General Hospital from March 1981 to July 1985. The following results were obtained: The peak of age incidence was the 5th decade amounting to 36.8%. The ratio between male and female was 2.6:1. The main chief complaint of each group was epigastric pain and it was followed by epigastric fullness. The most patient had the duration of symptom from 3 months to 6 months. For old-age group, mid-age group, major site of lesion in the antrum, meanwhile body for young-age group. The most frequent type of gastric cancer was type III in each group by Borrmanns classification. For each group, adenocarcinoma was major cases in the view of histopathological findings, and incidence of well differentiated type adenocarcinoma were lower in young-age group than others.

밝은社會運動의 時代的 當爲性과 基本精神

朴淳永,申大淳,趙萬濟 慶熙大學校 1983 論文集 Vol.12 No.-

The tasks of today's human society are 1) to preserve nature, 2) to create the sound social climate, 3) to construct human rehabitation and 4) to materialize the world peace with the human family spirit. The Bright Society Movement, acting up to such historical needs, is extending worldwidely. Following are the necessities of the times; 1) the population increase, the excessive urbanization and industrialization cause pollution to break the things of nature and to further intimidate human life. For that reason, the world-wide movement is urgently needed to protect the nature and revive her functions. 2) Materials are the necessary condition for man's life; nevertheless, the tendency spreads around the world to value more seriously materials than man. Machines and tools must be utilized for only man's convenience. It is our present mission to enhance human value and construct a society which is good to live. 3) As for human relationship, humanity and familiarity among neighbors grow more sterile. Recovering humanity in our society, we'll have to lead an intimate life with out neighbors as if a family, and all human beings ought to keep good relations one another under the awareness that we are all brothers. Since the world is becoming a global community, people in this community can not help co-operating one another. 4) The gradual increase of the number of modern people who take the passive and negative attitude in life, and antagonism between individuals, groups, races, and nations which most frequently results in war; these phenomena are typical of modern society. We should make every effort to induce people to take an active, positive attitude and cast away such antagonism. 5) Individualism and egoism for common prosperity. The solution is to place great emphasis collaboration and the basis for modern society are the main factors to destroy the social collaboration and the basis for common prosperity. The solution is to place great emphasis upon human education so as to make the habit of servicespirit. The Bright Society Movement, in accordance with these historical and social demands, takes as its motto Good will, Cooperation and Service―Contribution. 1. Good Will The spirit of Good Will is not only the native love made between friendly persons but the mental basis for human life. First, filial piety is the most important in the life of Good Will. Only a man, who is dutiful to his parents, can be a leader and do his best for society. Second, Good Will is beautiful love between husband and wife. For it is next to impossible without beautiful love to become the most intimate couple from two strangers. Third, Good Will is the love between parents and children. It is true love without any condition. Forth, Good Will is spirit of keeping friendly contact with neighbors. Such spirit is to help mutually, feel grateful, and live loving neighbors as if relatives. 2. Cooperation Cooperation is an essential principle to form human society. All beings in the universe keep inter-relation through the organic cooperative system with their environments. Man can yield greater results by mean of cooperation. A family can be happy when they collaborate one another; every member of a society is able to achieve their aims through their mutual cooperation. The Bright Society Club can construct human cooperative society as well, when it is possible to create the systematic cooperative relationship between unit clubs, federations, regional centres, national centres, and international centre. 3. Service-Contribution Service is man's natural behavior to contribute himself and the development of human beings. Everything with me is what is obtained from others, not what I produce by myself. Service means to reward everyone for what I have received. The society which consists of those who are willing to service each other; only that society can provide for proper opportunity to be compensated. Fruitful life does not lie in good health and materials. Really valuable Life is with helping others. Life, filled with Good Will, Cooperation, and Service-Contribution, must grow into a habit of everyman so as to be a guiding principle in his life. Now, the basic directions of unit club for the Bright Society Movement are as follows; 1. Leading role of unit clubs; Centering around unit clubs, Bright Society Movement is expanded. 2. Systematic relations of club structure; each unit club keeps organic relationship mutually. 3. Regional and functional unit clubs; unit clubs are formed on the basis of pecularities of regions or functions. When all human beings understand the historical and social missions and participate in the Bright Society Movement positively, we'll be able to creat earlier human cooperative society which is good to live.

태권도 앞굽이 몸통반대지르기를 통한 고령자의 건각도의 유효성 분석

김기만(Ki Man Kim),배영상(Young Sang Bae) 한국발육발달학회 2009 한국발육발달학회지 Vol.17 No.4

The purpose of this study was to identify effectiveness of Taekwondo Apkubi Momtong Bandae Jireugi (AMBJ) as diagnostic method of health related to muscle level of older men. To accomplish the purpose, relationship between a 3 stride of AMBJ distance and a cross section area of psoas major muscle were analyze. By using random sampling, 10 older men were sampled as subject who participate Gate-ball club in S-Gu, D-metropolitan city. Analysis of relation between the 3 stride of AMBJ and psoas major muscle scale recorded by MRI of older men were executed. Results of the analysis could be summarized as the following. 1) In the case of older men, the male who has much muscle weight and large thigh girth has big cross section area of psoas major muscle. 2) Relation among 3 stride of AMBJ, muscle weight(r=.822, p<.01), and thigh girth(r=.720, p<.05) especially, cross section area of psoas major muscle presented very high correlation(r=.91, p<.001). 3) Based on the analysis, measurement result of the 3 stride of Taekwondo Apkubi Momtong Bandae Jireugi was very effective diagnostic method to assume for health related to muscle strength level and all the muscular strength level of older men.

" The Korean Journal of Chemical Engineering " 게재 논문 요약 : Processabilities & Mechanical Properties of the Biodegradable LDPE / Modified Starch Blends

Young Jin Kim,Yong Man Lee,Hyang Mok Lee,O Ok Park 한국화학공학회 1994 NICE Vol.12 No.5

N/A An investigation of the biodegradable polymers based on starch has been accomplished. Starch needs to be modified before blending with low density polyethylene(LDPE) because of its hydrophilicity. Three kinds of modification methods were applied to obtain the compatibility to starch-LDPE blends. The first one was the esterification of the hydroxyl group of starch with acetic anhydride. The second was the copolymerization of actylonitrile and styrene onto starch. The last was the esterification reaction of the hydroxyl group of starch with three kinds of ionomers. The tensile strength, % elongation, Young`s modulus, and shear viscosities of blends of the modified starch and LDPE were examined. Ionomer-treated starch showed better compatibility with LDPE to give better performances than other blends.

진공열간가압성형 (VHP) 법 및 압출로 제조한 SiCp/Al 및 SiCp/2024Al 복합재료의 동적탄성계수와 감쇄능 (내부마찰) : 금속기 복합재료의 동적탄성계수 및 감쇄능 측정

김영만,조기현,이경민,홍성길 대한금속재료학회(대한금속학회) 1999 대한금속·재료학회지 Vol.37 No.9

To shorten the processes of MMC fabrication, a number of techniques are being studied. In this study, a new process for MMC's fabrication was developed utilizing vacuum hot pressing and subsequent extrusion. MMC's with pure Al and 2024Al matrices reinforced with various volume fractions of SiC particulates were produced at 400℃ by vacuum hot pressing(VHP), followed by direct extrusion with extrusion ratios of 10:1 at 480℃ at constant ram speed of 2㎜/sec. The effects of extrusion pressure and relative density according to the volume fractions of the reinforcement were compared with the resultant metallurgical microstructures. Young's moduli of the manufactured composites were measured by the sonic resonance method, damping capacity by free decay method. Measured Young's moduli of the composites at room temperature were compared with the predictions based on Rule of Mixtures, Halpin-Tsai and Mean-field models. Measured Young's moduli of the composites are in good agreement with the predictions based on Mean-field model. The composites reinforced with finer particles showed higher damping capacity under the same amount of SiC reinforcement.

곤충

장영덕,윤영남,장철,이만영 忠南大學校 環境問題硏究所 1992 環境硏究 Vol.10 No.-

1992년 9월 4일부터 5일 까지에 전라북도 진안군에서 조사된 곤충류는 총 9목 58과 176종이었다. 조사된 종류중 노린재목이 41종으로 가장 많았으며 딱정벌레류는 빈약한 편이었다. 또한 남부지방의 타지역에 비해 특기할 만한 종이나 현상은 없는 편이었다. 종다양도는 site A1에서 34.167로, 우점도는 site Y3에서 0.779로, 종풍부도는 site S1에서 4.327로 가장 높게 나타났으며 지역간 유사도는 인정되지 않았다.

직장 Dieulafoy 병변으로 다량의 혈변을 보인 1 예

김태영;이상헌;김지현;배영환;정은주;김승만;지삼룡;설상영 인제대학교 2011 仁濟醫學 Vol.32 No.-

Dieulafoy’s lesion is very rare cause of gastrointestinal bleeding, Deiulafoy’s lesion is exposure of non ulcerative, abnormally large tortuous, thick- walled artery in the muscularis mucosa through a small submucosal defect. It is usullay found on stomach antrum and lesser curvature side of the proximal body, and possibly found on any sites throughout whole gastrointestinal tract, but rarely on rectum. The principal of treating Dieulafoy’s lesion was the surgical resection of lesion, but recently remarkable advances in endoscopic technique make endoscopic approach more popular. We report a case of rectal Dieulafoy’s lesion successfully treated with endoscopic hemoclipping in an elderly female patient.

급성 골수성 백혈병 환자에서 발생한 침습성 아스페르길루스중에 대한 수술적치료 1 예

배영환;어원식;어상민;김승만 김태영;박수영;서영경;정은주;안효정 인제대학교 2011 仁濟醫學 Vol.32 No.-

Invasive pulmonary aspergillosis is known to have a poor prognosis and major cause of morbidity and motality in patients with hematologic disease. Without adequate therapy, invasive pulmonary aspergillosis will almost always progress to fatal pneumonia. Early administration of antifungal agents in patient with invasive pulmonary aspergillosis is most important. And surgical resection of aspergillous-infected tissue may be useful in patients with lesions that are contiguous with great vessel and lesions causing hemoptysis. We reported a rare case of surgical resection as curative treatment for invasive pulmonary aspergillosis patient in 58-year-old man who had the history of acute myeloid leukemia.

개에서 이중 결찰법을 통한 동맥관 개존증의 완치 예

윤헌영,정순욱,박희명,박철,정만복,김준영,한현정,황민,노병국,박상혁,장하영,박정윤 한국임상수의학회 2004 한국임상수의학회지 Vol.21 No.1

A ten months old, female Yorkshire terrier weighing 2.88 kg referred to veterinary teaching hospital of college of veterinary medicine, Konkuk University because of syncope, cough and dyspnea. First hematological and serum chemical test revealed thrombocytopenia, mild anemia, and increase of concentration of ALP (195 U/L). On 57 days later, second hematological and serum chemical test revealed polycythemia, increase of concentration of ALP (211 U/L), and Tchol (387 mg/dl). Right atrium enlargement, main pulmonary artery bulge and cardiomegaly (VHS = 11.5) were observed in radiographic findings. Ultrasonographic images showed both right and left ventricular dilation and turbulent flow between the descending aorta and the main pulmonary artery in color Doppler imaging. ECG showed left ventricular enlargement, SA block, and electrical alternant. Thoracotomy was performed through left fourth intercostal incision under isoflurane anesthesia. Patent ductus arteriosus was double ligated with 1-0 silk. Cough and dyspnea disappeared on 5 days after operation. Turbulent flow was not found in color doppler imaging of ultrasonography on 10 days after operation. Ten months later after the operation, syncope could not exist any more.