뱀장어 아기미 울혈증에 관한 연구

이근광,김영길,박성우,최민순 한국어병학회 1994 한국어병학회지 Vol.7 No.2

뱀장어의 울혈증 발병 원인구명을 위하여 서해안 지역 5 개 양만장을 대상으로 아가미 울혈증 발병현황과 수질등 역학조사를 실시하였고, 한편으로는 스트레스(handling 과 수온차) 및 병어에서 분리한 아가미 마쇄액을 건강어의 복강에 주사하여 울혈증 발병률과 CHSE-214 에 접종하여 세포변성을 조사하였다. 조사한 양만장의 아가미 울혈증 발병률은 30-80%로서 사육시의 수온과 선별시의 수온차가 클수록 발병률이 높았으며, 수질환경과 본증의 발병과는 뚜렷한 상관관계가 없었다. 또한 발병률이 높은 양만장의 뱀장어는 Ht, Hb, Tp, Alb, Mg 및 Glu 치등이 낮은 반면 GOT, GPT, Ca 및 Met-Hb 치등은 높게 나타나는 경향을 보였으나, 반드시 아가미 울혈증에 비례하여 증감되지는 않았다. 스트레스(handling 과 수온차)를 가함에 따라 아가미 울혈증이 실험적으로 유발되어졌으며, 발병률은 50-70% 이었다. 이러한 스트레스 조건하의 어류의 혈액학적인 성상은 울혈증 발병빈도에 비례하여 Ht, GOT, GPT, Met-Hb, Alb 및 Glu 치등은 증가되었으나, Tp, Hb 및 Mg 은 감소하는 경향을 보였으며, 온도차가 클수록 cortisol 량이 증가되는 경향을 보였다. 그러나, 병어의 아가미 마쇄액을 복강주사 및 CHSE-214 에 접종하였던 바 각각 울혈증의 발병 및 세포변성이 전혀 관찰되지 않았다. 이러한 결과는 선별시 handling 과 수온의 급변이 혈중 cortisol 을 상승시켜 혈액의 전해질 변화를 초래케하므로써 혈액의 흐름을 완만하게 되고 동정맥계로의 혈액유입량이 증가하게 되어 중심정맥동이 확장되므로써 울혈이 형성되는 것임을 시사한다. In order to elucidate the outbreak mechanisms of a new disease which is characterized by an intense congestion in central venus sinuses(CVS) of gill filaments in cultured eel. these experiments were carried out; epidemically surveyed on the cultured eel farms in the vicinity of Kunsan city and experimentaliy outbreaked the disease in the stressful condition such as thermal and handling shock and innoculated the supernatant from the homogenate of naturally severe congested gill into eels and onto the monolayer of the CHSE-214. Although the frequency of congestion in eels of B, C, D and E farms were higher than in eels of A farms, the water qualities (stocked and cultured water) among farms were not a great difference. In eels of B, C, D and E farms, the values of haematocrit (Ht), haemoglobin (Hb), total protein (Tp), albumin (Alb), glucose (Glu), magnesium (Mg) were lower and the values of calcium(Ca), methemoglobin(Met-Hb), glutamic pyruvic transminase (GPT), glutamic oxalacetic transminase (GOT) higher than in eels of A farms. These valules have not related to the frequency of congestion. An intensive congestion and dilataton in CVS of gill filaments in experimentally handling-stressed eels produced similar histopathological changes to those observed in the spontaneously diseased eel, but not in eels experimentally injected with filtering contents. The cytopathic effect on the CHSE-214 was not observed. In stressed eels the congestion of gill was increased in relation to either the decrease ranges of water temperature or the incerase in accllimated times. And increase in Ht, Met-Hb, Alb, Glu, GOT and GPT and decrease in Mg, Hb and Tp were found, which had a close relationship to congestion of gill. Cortisol were increased according to the decrease ranges in acclimated water temperature. From these results, decrease in water temperature during selection placed eels upon the stressed condition, made increase in ionic strength in blood stream, and CVS was dilatated owing to the increased blood inflow.

春季 錦江下流域의 水質 및 Diatom의 組成變化에 關하여

金榮吉,金鍾培 군산대학교 1978 群山水産專門大學 硏究報告 Vol.12 No.2

The monthly observation on the composition quantity of Diatoms and chemical contents was done at 7 stations in the below stream area of Gum River from April to June, 1978. The total numbers of Diatom appeared by observation were 27 species ; neritic planktons(27), and oceanic planktons(2) and the composition organism of total diatoms was 1,600Cells/ℓ∼41,700Cells/ℓ. The surface water temperature is 6.8∼20.8℃ at St. 3, 13.4∼23. 6℃ at St. 7 and the difference between two stations, was 3.3∼6.6℃, when, the temperature at St. 5∼7 was higher than 1∼4. Transparency was 3.2m at St. 7, 11m at St, 3 and salinity was 29, 9∼32.2% at St. 1∼4, 28.4∼29.7% at 5∼7. The surface water of dissolved oxygen content was 5. 3∼6.2cc/ℓ, and bottom water was 5.3∼5.9cc/ℓ. The content of C.O. D was 0.49∼1.2ppm and it was higher in April than it was in May and June. In case of NO2-N, the surface water was 0.3∼0.6㎍- at/ℓ, bottom water was 0.3∼0.6㎍- at/ℓ, and it's contents was the highest in June. The contents of PO4- P was 0.03∼0.0㎍- at/ℓ, SiO2 was 5.9∼10.1㎍- at/ℓ and Ammonia-N was 0.8∼1.6㎍ at/ℓ.

西海 沿岸의 養殖場 環境 調査 : 2. 高亭理 海域 김養殖場의 水質環境

金容浩,金榮吉,李廷烈,金鍾連 군산대학교 1990 群山水産專門大學 硏究報告 Vol.24 No.1

To understand the effects of oceanographic condition on the harvest of laver and establish the basic environmental data for cultivation farm of laver, a series of survey was carried out in Gojeong-ri sea area, west coast of Korea from January to September, 1987. Variation of water temperature in Gojeong-ri sea area was 3.8-25.8℃, pH was 7.8-8.4, and transparency was 1.3-3.1m. Salinity was changed from 19.57 to 31.99% and lower remarkably in July and August owing to a heavy rain. The concentration of DO in surface water wart varied from 9.4-10.1㎎/ℓ and in lower layer was 8.0-9.99㎎/ℓ . COD was 0-4.28㎎/ℓ by alkalic modification of potassium permanganate method. Distribution of total suspended solid was 13.7-488.7㎎/ℓ and the rate of light penetration in 1m water column was 7.2-15.5%. The concentration of nutrients were changed in 0.48-23.63㎍-at./ℓ for ammonia nitrogen, 0-4.45㎍-at./ℓ for nitrite nitrogen, 0.39-3.13㎍-at./ℓ for nitrate nitrogen, 0-21.42㎍-at./ℓ for phosphate, and 0.84-157.32㎍-at./ℓ for silicate. It was showed high concentration in summertime, raining season in general. The concentration of chlorophyll a was 0.39-20.62㎎/㎥. Total 57 species of phytoplankton and 21 groups of zooplankton were identified from collected samples. Dominant species were Coscinodiscus radiatus, Eucampia zodiacus as a phytoplankton and Noctiluca scinitillans, Cladocera, Copepods sp. as a zooplankton. The total standing crop of phytoplankton was ranged of 5,500-1,524,400cells/ℓ and that of zooplankton was 3,000-43,000 individuals/㎥.

금강하구산 어패류에 기생하는 흡충류에 관한 연구 : 一報 문절망둑에서 검출되는 Echinostoma속 흡충에 관하여

金榮吉 군산대학교 1975 群山水産專門大學 硏究報告 Vol.9 No.2

In the intestine of 59 fishes of Acanthogobius flavimanus (T. et SCHLEGEL) gathered from Sun yearn-Ri, the lower cours of the Kum river over 5 times from March till July in 1975, 5 Encysted larvae of Echinostama sp were detected. The size of Encysted larvae is 650×610μ and Encysted larvae 1,750×475μ. Head collar arranged in 2 lines composed of 37.

조개類에 寄生하는 Bacciger屬 吸忠類에 關한 硏究 - Ⅱ Latermla limicola REEVE에서 檢出되는 Bacciger harengulae에 대하여

金榮吉 군산대학교 1982 群山水産專門大學 硏究報告 Vol.16 No.1

Cercaria larvae of Bacciger parasitic to the littoral spoon clam, Laternula limicola REEVE were studied in terms of their morphology and incidence of infection rate. The host bivalves were examined from Naechodo, Gunsan from September 1981 to February, 1982, The cercaria were morphologically compared to those found in the others clams, i.e. Meretrix lusoria, Tapes philippinarum, and Solen strictus. It is conformed that the cercariae were identified to be Cercaria harengulae. Head size of the cercaria was 250X150μm, and tail size was 480X57μm. The tail was armed with 27 pairs of tufts of setae, the tuft being composed of 6∼8 fin rays. Oral sucker, ventral sucker, intestine and a pair of testis were distinctive. The flam cell arrangement is represented by a formula, 2[3+3)+(3+3)]=24. Mean infection rate to L. limicola was 14.8%. However the lager bivalves showed the higher infection rate. In conclusion L. limicola is newly added to the hosts of Cercaria harengulae of Bacciger sp.

非營利法人 會計制度의 會計情報 效率性 提高方案 : 교회회계제도를 중심으로 Chiefly the Church Accounting System

박길영 조선대학교 경영경제연구소 1995 經營經濟硏究 = Management and economics research Vol.18 No.1

古群山 列島産 貝類目錄

金榮吉 군산대학교 1974 群山水産專門大學 硏究報告 Vol.8 No.2

I have collected the Molluscan shells at the Go-Gun San Islands in the Western Sea. List in recorded are as follows: 1.Total species in appearance are 73, such as Loricates 1, Gastropoda 37, Lamellibranchia 35. Gastropoda and fixed sedentary is distributed much more than submerged sedentary. 2.Brevimyurella lischkeana(PHILIPPI), Euhadra congenita hikonis (KO-BELT), Rapana bezoar(LINNEUS) are Listed in Unrecorded species in Korea.

한국산 어류에 기생하는 포자충에 관한 연구 : 1. 메기(Silurus asotus)와 문절망둑(Acanthogobius flavimanus)에서 검출된 포자충 1. Myxosporidian parasites from catfish (Silurus asotus) and Goby (Acanthogobius flavimanus)

김영길,박성우,최민철 한국어병학회 2002 한국어병학회지 Vol.15 No.3

한국산 어류에 감염된 포자충의 종류와 병해를 조사 할 목적으로 2001년 9월부터 2002년 8월까지 양식중인 정읍산 메기와 만경강 하구의 기수 구역에 서식하는 자연산 문절망둑을 조사하였다. 이들 어류에서 한국 미기록종인 포자충의 감염이 확인되었다. 즉 메기의 장상피조직에서 Myxobolus miyairii Kudo, 1919가, 문절망둑의 피하 근육조직에서 Henneguya tridentigeri Ozaki & Ishizaki, 1941가 검출되었다. 메기에 기생 된 M. miyairii는 장 상피조직에 42~77(56)㎛ × 59~93(73)㎛ 크기의 백색 영양체가 무수히 관찰되었고, 포자의 길이는 11.0~12.0(11.3)㎛이었다. 어체 기생율은 86.7~90%였으나, 감염어는 별다른 병변을 발견 할 수 없었다. 문절망둑에 기생 된 H. tridentigeri는 몸통부 근육에 백색 타원형의 영양체를 형성하고 있었으며, 크기는 1.04~4.45(2.43)×0.43~2.21(0.95)mm이었다. 포자는 장 타원형으로 포자각의 후각에 2개의 돌기를 그 크기는 10.0~12.0(10.7)×6.0~8.0(7.1)㎛, 돌기의 길이는 12.0~24.0(16.9)㎛이다. 문절망둑에서의 기생율은 76.9~90.9%이었고, 감염어는 외관상 별다른 이상이 발견되지 않았다. Thirty five catfish (Silurus asotus) cultured in a fish farm of Jungeup and 222 wild goby (Acanthoguobius flavimanus) caught from the estuary of Mankyoung River, Chonbuk province of Korea were examined the prevalence of myxosporidian infestation. A lot of while spherical myxosporidian cysts in the intestinal epithelium of catfish and the muscle of goby were easily found. The cysts size was 56 × 73 (42~77 × 59~93 ㎜) in catfish and 2.43 × 0.95 ㎜ in goby. The infection rate(%) was 88.4 in catfish and 89.4 in goby. The Myxosporidians from the intestine of catfish and from the musculature of goby were identified as Myxobolus miyairii and Henneguya tridentigeri, respectively, on the basis of the shape and structure of the spores under a light microscope. Dimension (㎜) of fresh spores of Myxobolus miyairii: length, 11.0~12.0; width, 5.0~6.0; thickness, 3.5~4.0; polar capsule: length, 5.0~5.5; thickness, 1.5~2.0; polar filament: length, 40~45. Dimensions (㎛) of fresh spore of Henneguya. tridentigeri: length, 10.0~12.0; width 6.0~8.0; thickness, 4.8; polar capsule: length, 4.6; thickness, 2.6; iodinophilic vacuole: 2.8 × 2.4. No significant pathological change was found from the infected fish. This is the first report on M. miyairii and H. tridentigeri from fish species in Korea.

防潮堤의 壓密沈下量 解析에 관한 考察

安鍾弼,朴吉鉉,金永先 조선대학교 국토개발연구소 1988 국토개발연구 Vol.8 No.1

In Seadyke design a surface stress Calculation using osterberg chart for few point has been generally used to settlement quantity Computation. This study is developed to the Computer Program of integral formula having a Zonal triangular load, A few Case studies are examinedand find out degree of error about the Cross section. This results can be use to the seadyke design.

대맛조개, Solen grandis(Dunker)의 生殖細胞 形成過程 및 生殖周期

鄭義泳,金榮吉 群山大學校自然科學硏究所 1986 自然科學硏究 Vol.1 No.-

韓國産 맛조개류中 크기가 가장 크며 水産業上 重要한 位置를 차지하고 있는 대맛조개, Solen grandis를 對象으로 生殖細胞 形成過程 및 生殖周期를 組織學的으로 調査하였고 이들의 增養殖을 爲한 基礎資料를 제공하기 爲해 生殖生物學的 調査를 하였다. 1. 대맛조개는 雌雄異體로서 卵生이다. 生殖巢는 內臟囊의 肝中腸線下方으로부터 足部의 纖維性網狀結締組織까지 分布되어 있다. 2. 卵巢는 數많은 卵巢小囊으로 構成되어 있으며 卵巢小囊의 上皮는 生殖上皮의 機能을 하고 있다. 卵原細胞는 生殖上皮上에서 어린 卵母細胞가 成長하고 있다. 精巢 역시 수많은 精巢小葉으로 構成되어 있으며 精巢小葉上皮는 生殖上皮의 기능을 한다. 小葉上皮를 따라서 精原細胞들이 活潑하게 增殖하고 있다. 3. 初期分裂增殖中인 卵原細胞는 10μm內外의 크기로 核과 仁이 뚜렷하고, 初期 成長中인 卵母細胞는 卵柄을 形成하여 生殖上皮에 부착하였다. 4. 數많은 未分化間充織과 好酸性顆粒細胞들은 初期發達期에 成長中인 卵母細胞와 精母細胞사이에 풍부하게 分布하나 卵巢와 精巢가 좀더 發達해감에 따라서 이들 間充織과 好酸性顆粒細胞들은 점차로 사라져 이들 組織과 細胞들은 營養細胞로 간주된다. 5. 成熟卵母細胞들은 卵巢小囊의 內腔內에서 원형 또는 타원형으로 되며, 完熟卵의 크기는 80∼90μm이었다. 그리고 精巢小葉上皮上에는 精原細胞 精母細胞, 精細胞 및 變態한 精子의 順으로 重層配列을 하였다. 6. 生殖年周期는 12月부터 1月까지 分裂增殖期, 1月부터 3月까지 成長期, 3月에서 8月까지 成熟期, 6月에서 7月까지 放出期 그리고 7月에서 12月까지 退化 및 休止期 등 連續的인 周期로 區分할 수 있었고 産卵盛期는 7月이었다. 7. 産卵期는 水溫과 密接한 關係를 가지며 水溫 20℃ 以上에서 産卵이 일어나고 있다. 8. 年間 肥滿度 및 消化盲囊의 脂肪細胞內 脂肪함량의 變化는 生殖年周期와 密接한 關係를 가지며 變하였다. 9.암컷과 수컷의 群成熟度는 殼長 8.0∼8.9 cm에서 50% 이상이었고, 10.0∼10.9 cm에서가 100%이었다. 全個體의 50% 이상이 再生産에 가담하기 시작하는 개체들의 연령은 滿 2歲부터 이었다. The structure of gonads, gametogenesis and reproductive cycle of the razor clam, Solen grandis were investigated by histological observation, and studied by comparing various quantitative variables, such as seasonal changes of fatness, the liver cell of the digestive diverticulum, and the first maturity. The materials were monthly collected at the coastal area of Yoobu-Do, Chungcheong Namdo, Korea, for one year from February 1985 to January 1986. 1. Sexuality of Solen grandis is dioecious, and the species is oviparous. The gonads are irregularly arranged from the subregion of mid-intestinal gland in visceral cavity to reticular connective tissue of foot. 2. The ovary is composed of a number of small ovarian sacs. The epithelium of ovarian sac has a function of the germinal epithelium. Oogonia actively proliferate along the germinal epithelium of the ovarian sac, in which young oocytes are growing. The testis is composed of a number of testicular lobuli, and the epithelium of the testicular lobule has function of germinal epithelium, along which spermatogonia actively proliferate. 3. Early multiplicating oogonium was about 10 μm in diameter. Nucleus and nucleolus were distinct in appearance. Each of the early growing oocytes made an egg-stalk, connected to the germinal epithelium. 4. A great number of undifferentiated mesenchymal tissue and eosinophilic granular cells are abundantly distributed between the growing oocytes and spermatocytes in the early development stages. With the further development of the ovary and testis, these tissues and cells gradually disappear. Then the undifferentiated mesenchymal tissue and granular cells are considered to be related to the growing of the oocytes and spermatocytes as nutritive cells. 5. Mature oocytes gradually become round or oval in the lumen of ovarian sac. Ripe oocytes were about 80 to 90 μm in diameter. With the further development of gonad, each of the testicular lobli formed stratified layers composed of spermatogonia, spermatocytes, spermatids and spermatozoa in groups on the germinal epithelium. 6. The reproductive cycle of this species could be classified into five stages; Multiplicative from December to January, growing from January to March, mature from March to August, spent from June to July with peak spawning in July, degeneration and resting stage from July to December. 7. It seems that spawning season is closely related to the water temperature, and the spawning of Solen grandis occurs at above 20℃ in water temperature. 8. The annual variation of fatness of Solen grandis correlated with gonadal phases, was remarkably decreased by spawning. It seems that changes of the volume of lipid in the lipid cell of digestive diverticulum is closely related to the reproductive cycle also. 9. Percentages of the first maturity in female and male clams ranging from 8.0 to 8.9 cm were over 50% and from 10.0 to 10.9 cm in shell length 100%. Both sexes participated in reproduction from two years old.