韓國産 자작나무科의 系統分類學的 硏究(序論)

李相泰,蘇雄永,田寬培,李愚喆 全北大學校 生物學硏究所 1981 生物學硏究年報 Vol.2 No.-

The present paper is an introduction to the whole project of "The systematic studies on Korean Betulaceae". Three studies under the project were performed this year and presented in this report : a review of taxonomic literatures and the construction of distribution maps by Woo-Tchul Lee, pollen morphology by Sang-Tae Lee and Moo-Yeol Kim, and wood anatomy by Woong-Young Soh and Kwan-Bae Jeon.

자작나무科의 導管形態에 依한 分類

蘇雄永,田寬培 全北大學校 生物學硏究所 1981 生物學硏究年報 Vol.2 No.-

To determine the degrees of specialization and intergeneric relationship within the Korean Betulaceae a comparative anatomy was performed with 3-4 year-old parts of growth rings of the stem wood from a total of 18 species and 7 varieties of the family. Some specialized anatomical characters suggests the family to be more or less advanced. Tribe Betuleae possessing scalariform perforation plates exclusively, steeper angle of end wall and alternate pittings, was thought to be more primitive than tribe Coryleae. Among the Betuleae, Alnus exhibited to be more primitive than Betula on the bases of the number of bars on the perforation plate, the angle of end wall and the pitting pattern. Among the coryleae, it was thought that Corylus having a scalariform perforation plate exclusively, and a intermediate number of bars was the most primitive, whereas both Carpinus and Ostrya having simple perforation plates and homogeneous I ray were the most advanced.

Agrobacterium을 이용한 고추의 형질전환체계개발

최근원,박영두,박승국,전영주,유일웅,김인태,백운돈 경희대학교 생명자원과학연구원 1997 硏究論文集 Vol.18 No.-

본 실험은 고추의 형질전환체 선발을 위한 최소의 항생제농도를 결정하고 그 결과를 이용하여 형질전환체를 유기하고 효율을 최대화하기 위하여 수행한 바 그 결과는 다음과 같다. 1. 항생제 감응성 검정결과 kanamycin 40㎎/ℓ와 hygromycin 20 ㎎/ℓ에서 신초형성이 완전히 억제되었다. 2. Agrobacterium 제거에 사용되는 carbenicillin과 cefotaxime 처리시 재분화에 아무런 영향을 미치지 않았으며 cefotaxime 200㎎/ℓ 처리에서는 무처리구보다 신초형성이 더 활발하였다. 3. 회복친인 135Q와 276F에서는 형질 전환시 높은 재분화율을 보였으나 형질전환율은 각각 10%와 11.1%의 낮은 빈도를 보였다. 4. 재분화된 신초들을 대상으로 histochemical GUS assay를 실시한 결과 부분적으로 푸른발색이 확인되었다. 5. GUS활성을 보인 신초들에 대한 NPTⅡprimer를 이용한 PCR검정에서 NPTⅡ 유전자의 존재가 확인되었다. A transformation system using Agrobacterium tumefaciens was developed with different genotypes of hot peppers (Capsicum annuum). Four antibiotics commonly used in Agrobacterium-mediated transformation were tested for their effects on shoot regeneration. Shoot induction was completely inhibited by kanamycin at 40㎎/ℓ and hygromycin at 20㎎/ℓ or higher, but not by carbenicillin and cefotaxime. Cotyledon explants of hot peppers were cocultivated with Agrobacterium turnefaciens LBA4404 which carries a binary. vector pBI121 harboring the neomycin phosphotransferase, type Ⅱ (NPT Ⅱ) gene and β-glucuronidase (GUS) gene. For selection of transformed cells, 40㎎/ℓ kanamycin were used because the sensitivity test showed that 40㎎/ℓ kanamycin completely inhibited shoot induction from nontransformed cells. Histochemical GUS assay showed the presence of the GUS gene and PCR analysis confirmed the NP TⅡ gene was integrated. Transformation frequency was about 10.0% and 11.1% of total regenerants from 135Q and 276F, respectively.

독성물질의 세포사 기전 및 세포사 유발물질의 검색법 개발에 관한 연구(Ⅱ) : 망간 신경독성을 이용한 파킨슨증 모델의 세포사 Studies on the Mechanism of Parkinsonism induced by Manganese

김종민,박창원,오정자,이보경,서경원,서수경,김규봉,김종원,김광진,김영옥,전범석,박찬웅,이선희 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

1960년대 이후 파킨슨병의 유발물질 중 한 후보로서 망간이 주목받으면서 많은 역학적, 병러학적 연구가 수행죄브다. 그러나 망간이 파킨슨병을 직접 일으키는지, 혹은 파킨슨병과 관련된 부위의 신경세포를 파괴시쿡 파킨슨증만을 초래하는 것인지가 아직 정립되지 않은 실정이다 본 연구에서는 실험동물모델에서 행등학적 변화 측정 및 병리학적, 샐화학적 연구를 통하여 망간의 과다노출글 따른 파킨슨병 유발 여부를 확인하고자 하였다. Sprague-Datylet· 렛드에 망간을 1, 2, 5, 10 mgAg/day의 응량으로 30일 등안 복강 투여하였을 때 모든 망간투여군에서 운동성 감소가 관찰되었다. 뇌조직의 망간 함량을 ion chromatograph?로 측정한 결과 중뇌 흑질과 기저핵 선조체에서 망간 함량의 뚜렷한 증가 소견이 관찰되었으며, 니슬염색체서 선조체의 신경세포수의 유의성 있는 감소가 관찰되었다. 그러나, 흑질의 TH효소 면역염색, GFAP 면역염색, 흑질과 선조체의 T릿효소 western blot 결과는 대조군과 차이가 없었다. 이상의 결과에서, 망간-파킨슨증 모델에서 파킨슨증어 유발되는 기전은 흑질 도파민성 신경 세포의 신호를 받는 선조체 씬경세포의 사멸에 의한 것으로 생각된다. 결론적으로, 망간 독성에 의한 파킨스증 모델에서는 파킨슨 병과는 달리 흑질 도파민성 신경세포의 세포사는 관여하지 않는 것으로 관찰되었다. Manganese(Mn) intoxication causes a parkinsonian syndrome. It may be difficult to distinguish Mn-induced parkinsonism from idiopathic Parkinson disease(IPD). Neuropathological descriptions on the brains with Mn intoxication showed the perferential damage in the globus pallidus and substantia nigra pars reticularis. Pathological reports on the substantia nigra pars compacta(SNpc), a mafor focus of pathologic changes in IPD, are discordant and controversial. The SNpc involvement is of critical importance for the elucidation of pathogenesis of IPD. Therefore, the neurodegeneration in SNpc was investigated in the experimental model of Mn neurotoxicity. Sprague-Dawley rats were administered with manganese chloride(1, 2, 5, 10 mg/kg/day, i.p.) for 30 days. Treated animals showed low levels of distance-traveled from locomotor activity tests. Ion chromatography revealed that Mn accumulation was significant in SN and basal ganglia in Mn-treated animals. Nissl staining showed neuronal loss of the striatum in all treated animals. The degree of neurodegeneration in SN was determined by immunohistochemical staining of tyrosine hydroxylase(TH). The numbers of TH-positive cells on nigral sections were not different from each groups(P>0.05). The densities of glial fibrillary acidic protein immunoreactivity from SN were similar between groups. TH-westen blotting showed no differences between treated animals and controls. In conclusion, the SNpc remains intact in Mn intoxication, and Mn-induced parkinsonism may be caused by damages of output pathways downstream to the nigrostriatal dopminergic system.

ADSORPTION BEHAVIOR OF ASPHALTIC MODEL COMPOUNDS ON SILICEOUS SANDS

Jeon, Young-Woong 경북대학교 산업기술연구소 1994 産業技術硏究誌 Vol.22 No.-

석유아스팔트는 도로건설이나 석유 또는 타르로 오염된 토양물질을 안정화하는 데에 사용되고 있다. 아스팔트와 광물질의 계면에서 일어나는 물리화학적 현상은 두물질간의 결합을 형성하는데 매우 중요함으로 많은 연구의 대상이 되어 왔다. 본 실험에서는 질소, 산소, 황과 같은 이형원자들로 구성된 아스팔트 모델작용기들이 25 ℃ 조건에서 실리카와 실리카를 주 성분으로 함유하는 경사암 입자표면에 흡착하는 현상을 관찰하였다. 사용된 작용기는 각각 벤조산(칼복실기), 1-나프톨(페놀기), 페닐설폭사이드(설폭사이드기), 페난트리딘(피리딘기), 9-플루오리논(케톤기) 그리고 인돌(파이롤기)이다. 이러한 작용기들의 흡착평형 현상은 Langmuir와 Freundlich 흡착모델에 의하여 잘 설명되었다. 흡착모델에 의하여 결정된 흡착매개변수들(예를들면, monolayer capacity와 Gibbs free energy of adsorption)로 부터 실리카와 경사암 입자에 대하여 상호 유사성이 관찰되었다. 벤조산, 나프톨과 같은 산성 작용기들 그리고 페닐설폭사이드, 페난트리딘과 같은 염기성 작용기들이 플루오리논과 인돌의 중성 작용기들에 비하여 강하게 흡착하였다. 사용된 여섯가지 작용기들 중에서 페닐설폭사이드가 실리카와 경사암입자에 대하여 가장 강한 흡착친화력을 나타내었다. 또한, 경사암입자가 실리카와 유사한 흡착현상을 보이는 것을 근거로 하여 경사암 입자에 대한 아스팔트 작용기들의 다성분 경쟁흡착평형을 단성분 흡착결과로 부터 이상흡착용액이론(ideal adsorbed solution theory)을 적용하여 예측하였다.

Adsorption of Organic Compounds onto Mineral Substrate Prepared from Oyster Shell Waste

Jeon, Young-Woong,Jo, Myung-Chan,Noh, Byeong-Il,Shin, Choon-Hwan The Korean Environmental Sciences Society 2001 Environmental sciences Vol.10 No.2

Humic acids react with chlorine to produce Trihalomethanes(THMs), known as carcinogens, during disinfection, the last stage in water purification. Currently, the removal of organic humic acids is considered the best approach to solve the problem of THM formation. Accordingly, the current study examined the adsorption of organic compounds of humic acids onto an inorganic carrier prepared from oyster shell waste. The adsorbent used was activated oyster shell powder(HAP) and silver ion-exchanged oyster shell powder(HAP-Ag), with CaCO$_3$ as the control. The adsorbates were phthalic acid, chelidamic acid, catechol, dodecylpyridinium chloride(DP), and 2-ethyl phenol(2-EP). The adsorption experiments were carried out in a batch shaker at $25^{\circ}C$ for 15 hours. The equilibrium concentration of the adsorbate solution was analyzed using a UV spectrophotometer and the data fitted to the Langmuir isotherm model. Since the solution pH values were found to be greater than the pKa values of the organic compounds used as adsorbates, the compounds apparently existed in ionic form. The adsorptive affinities of the organic acid and phenolic compounds varied depending on the interaction of electrostatic forces, ion exchange, and chelation. More carboxylic acids and catechol, rather than DP and 2-EP, were adsorbed onto HAP and HAP-Ag. HAP and HAP-Ag exhibited a greater adsorptive affinity for the organic compounds than CaCO$_3$, used as the control.

Middleware Construction for Clinical Stroke MR Images

Woong-Gi Jeon,Young-Seung Lee,Hyun-Ju Choi,Young Huh,Sang-Wug Jeong,Dong-Eog Kim,Heung-Kook Choi 한국멀티미디어학회 2011 한국멀티미디어학회 국제학술대회 Vol.2011 No.-

In this paper we have studied a middleware construction for an effective medical data communication. Brain MR￢images were based on the stroke clinical research system. Our proposed middleware consists of reusable sources at each separated functions. A measurement communication access time by 100 clinical trial medical images we obtained an effective result significantly. In the future this middleware importance will be increased to the medical database construction.

근치적 위절제술을 받은 위배출구 폐색증을 동반한 위암 환자의 임상 분석

전영웅(Young Woong Jeon),이제희(Je Hee Lee),김종흥(Jong Heung Kim),박종민(Jong Min Park) 대한외과학회 2008 Annals of Surgical Treatment and Research(ASRT) Vol.74 No.2

Purpose: This study was conducted to analyze the clinical course of patients of gastric cancer with outlet obstruction by comparing them with the gastric cancer patients without outlet obstruction. Methods: The records of gastric cancer patients with outlet obstruction and who underwent radical gastrectomy from January 1996 to December 2005 were retrospectively reviewed and compared with those patients with gastric cancer and who were without outlet obstruction. Results: The number of the patients who underwent radical gastrectomy was 461. Out of 461 patients, the number of gastric cancer patients with outlet obstruction was 42 (9.1%), and they had nasogastric tube insertion for an average of 4.4 days before operation. These patients showed several significant features such as large tumor size, advanced stage, an increased the number of involved LNs, a low serum albumin level, a frequent antral tumor location and duodenal invasion. There was no difference in complication rates between the two groups, but a higher mortality rate was noted in the gastric cancer patients with outlet obstruction than that in the other patients (P=0.038). The survival rate after the operation was significantly lower among the gastric cancer patients with outlet obstruction (P=0.0008), whereas no difference was found in the survival rate between the above two groups at the same stage (P=0.1951). Conclusion: The gastric cancer patients with outlet obstruction showed a comparatively poorer prognosis than those gastric cancer patients without outlet obstruction. However, there was no significant difference in the complication rate and the survival rate for both types of patients who were at the same stage. These results suggest that the patients with outlet obstruction can show the same result as the patients without outlet obstruction by undergoing radical gastrectomy.

Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases

Sung, Young Hoon,Kim, Jong Min,Kim, Hyun-Taek,Lee, Jaehoon,Jeon, Jisun,Jin, Young,Choi, Jung-Hwa,Ban, Young Ho,Ha, Sang-Jun,Kim, Cheol-Hee,Lee, Han-Woong,Kim, Jin-Soo Cold Spring Harbor Laboratory Press 2014 Genome Research Vol.24 No.1

<P>RNA-guided endonucleases (RGENs), derived from the prokaryotic Type II CRISPR-Cas system, enable targeted genome modification in cells and organisms. Here we describe the establishment of gene-knockout mice and zebrafish by the injection of RGENs as Cas9 protein:guide RNA complexes or <I>Cas9</I> mRNA plus guide RNA into one-cell-stage embryos of both species. RGENs efficiently generated germline transmittable mutations in up to 93% of newborn mice with minimal toxicity. RGEN-induced mutations in the mouse <I>Prkdc</I> gene that encodes an enzyme critical for DNA double-strand break repair resulted in immunodeficiency both in <I>F</I><SUB>0</SUB> and <I>F</I><SUB>1</SUB> mice. We propose that RGEN-mediated mutagenesis in animals will greatly expedite the creation of genetically engineered model organisms, accelerating functional genomic research.</P>

만성 간 질환에서 Thallium-201 간 스캔의 단락지수(Shunt Index)와 간 조직 검사 소견에 대한 연구

유영조(Young Jo Yoo),정진웅(Jin Woong Jung),최춘식(Choon Sik Choi),전대원(Dae Won Jeon),이오영(Oh Young Lee),최호순(Ho Soon Choi),윤병철(Byung Chul Yoon),함준수(Joon Soo Hahm),이민호(Min Ho Lee),최윤영(Yun Young Choi),홍은경(Eun Kyun 대한내과학회 2000 대한내과학회지 Vol.58 No.2

N/A Background : The disturbances of portal circulation in chronic liver disease may cause hepatic failure, hepatic encephalopathy and variceal bleeding. The measure of porto-systemic shunt plays a significant role in the management and prognosis of the patients. So we have evaluated the relationship between the shunt index of thallium-201 liver scan and the histological grade and stage of chronic liver disease. Methods : The thallium-201 scintigraphy per rectum was evaluated in 159 patients with chronic liver disease, which were proven with percutaneous liver biopsy. We used the heart to liver activity ratio at 20 minute as shunt index, representing portal-systemic shunt. The two pathologists scored independently hepatitis activity (lobular and porto-periportal activity) and stage (fibrosis). Results : A significant difference was noted between the shunt index and the scores of fibrosis (p<0.001) although this correlation was statistically weak (r=0.26, p=0.008). In cumulative logistic regression test, the shunt index had a effect on the fibrosis (p<0.001) but not on the lobular and porto-periportal activity. Fibrosis was predicted as less than 2 if shunt index was less than 0.24, 3 if more than 0.24 but less than 0.46, 4 if more than 0.46. Conclusion : The shunt index of thallium-201 liver scintigraphy correlated only with fibrosis not with lobular and porto-periportal activity. As the fibrosis progresses in chronic liver disease, portal hypertension becomes more severe and the shunt index increases. Thallium-201 liver scan may be useful for evaluation of hepatic fibrosis instead of invasive liver biopsy in predicting the histological stage (fibrosis) of advanced chronic liver disease.(Korean J Med 58:152-160, 2000)