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- 검색키워드
- 저자 : Youn D. H. (검색결과 305 건)
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PHF2 histone demethylase acts as a tumor suppressor in association with p53 in cancer
Lee, K-H,Park, J-W,Sung, H-S,Choi, Y-J,Kim, W H,Lee, H S,Chung, H-J,Shin, H-W,Cho, C-H,Kim, T-Y,Li, S-H,Youn, H-D,Kim, S J,Chun, Y-S Macmillan Publishers Limited 2015 Oncogene Vol.34 No.22
Plant homeodomain finger 2 (PHF2) has a role in epigenetic regulation of gene expression by demethylating H3K9-Me2. Several genome-wide studies have demonstrated that the chromosomal region including the PHF2 gene is often deleted in some cancers including colorectal cancer, and this finding encouraged us to investigate the tumor suppressive role of PHF2. As p53 is a critical tumor suppressor in colon cancer, we tested the possibility that PHF2 is an epigenetic regulator of p53. PHF2 was associated with p53, and thereby, promoted p53-driven gene expression in cancer cells under genotoxic stress. PHF2 converted the chromatin that is favorable for transcription by demethylating the repressive H3K9-Me2 mark. In an HCT116 xenograft model, PHF2 was found to be required for the anticancer effects of oxaliplatin and doxorubicin. In PHF2-deficient xenografts, p53 expression was profoundly induced by both drugs, but its downstream product p21 was not, suggesting that p53 cannot be activated in the absence of PHF2. To find clinical evidence about the role of PHF2, we analyzed the expressions of PHF2, p53 and p21 in human colon cancer tissues and adjacent normal tissues from patients. PHF2 was downregulated in cancer tissues and PHF2 correlated with p21 in cancers expressing functional p53. Colon and stomach cancer tissue arrays showed a positive correlation between PHF2 and p21 expressions. Informatics analyses using the Oncomine database also supported our notion that PHF2 is downregulated in colon and stomach cancers. On the basis of these findings, we propose that PHF2 acts as a tumor suppressor in association with p53 in cancer development and ensures p53-mediated cell death in response to chemotherapy.
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Lee, H J,Lee, Y N,Youn, H-N,Lee, D H,Kwak, J H,Seong, B L,Lee, J B,Park, S Y,Choi, I S,Song, C S Poultry Science Association, etc 2012 Poultry science Vol.91 No.1
<P>Polyphenolic compounds present in green tea, particularly catechins, are known to have strong anti-influenza activity. The goal of this study was to determine whether green tea by-products could function as an alternative to common antivirals in animals compared to original green tea. Inhibition of viral cytopathic effects ascertained by neutral red dye uptake was examined with 50% effective (virus-inhibitory) concentrations (EC??)determined. Against the H1N1 virus A/NWS/33, we found the anti-influenza activity of green tea by-products (EC?? = 6.36 ?g/mL) to be equivalent to that of original green tea (EC??= 6.72 ?g/mL). The anti-influenza activity of green tea by-products was further examined in mouse and chicken influenza infection models. In mice, oral administration of green tea by-products reduced viral titers in the lungs in the early phase of infection, but they could not protect these animals from disease and death. In contrast, therapeutic administration of green tea by-products via feed or water supplement resulted in a dose-dependent significant antiviral effect in chickens, with a dose of 10 g/kg of feed being the most effective (P < 0.001). We also demonstrated that unidentified hexane-soluble fractions of green tea by-products possessed strong anti-influenza activity, in addition to ethyl acetate-soluble fractions, including catechins. This study revealed green tea by-product extracts to be a promising novel antiviral resource for animals.</P>
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Kim, D.S.,Choi, J.S.,Kim, D.W.,Kim, K.S.,Seo, Y.G.,Cho, K.H.,Kim, J.O.,Yong, C.S.,Youn, Y.S.,Lim, S.J.,Jin, S.G.,Choi, H.G. Elsevier/North Holland 2016 International journal of pharmaceutics Vol.511 No.1
The purpose of this study was to compare the powder properties, solubility, dissolution and oral absorption of solvent@?wetted (SWSD) and kneaded (KNSD) l-sulpiride@?loaded solid dispersions. The SWSD and KNSD were prepared with silicon dioxide, sodium laurylsulfate and D-α-tocopheryl polyethylene glycol 1000 succinate (TPGS) using a spray dryer and high shear mixer, respectively. Their powder properties, solubility, dissolution and oral absorption were assessed compared to l-sulpiride powder. The drug in SWSD was in the amorphous state; however, in KNSD, it existed in the crystalline state. The SWSD with a drug/sodium laurylsulphate/TPGS/silicon dioxide ratio of 5/½/12 gave the higher drug solubility and dissolution compared to the KNSD with the same composition. The oral absorption of drug in the SWSD was 1.4 fold higher than the KNSD and 3.0 fold higher than the l-sulpiride powder (p<0.05) owing to better solubility and reduced crystallinity. Furthermore, the SWSD at the half dose was bioequivalent of commercial l-sulpiride@?loaded product in rats. Thus, the SWSD with more improved oral absorption would be recommended as an alternative for the l-sulpiride@?loaded oral administration.
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Lim, T-H,Kim, M-S,Jang, J-H,Lee, D-H,Park, J-K,Youn, H-N,Lee, J-B,Park, S-Y,Choi, I-S,Song, C-S Poultry Science Association, etc 2012 Poultry science Vol.91 No.1
<P>Infectious bronchitis virus (IBV) infections cause great economic losses to the poultry industry worldwide, and the emergence of new variant strains complicates disease control. The present study investigated the genetic and protectotypic features of newly emerged Korean IBV strains. A phylogenetic analysis showed that several recent isolates formed 2 different clusters (new cluster 1 and 2), which were distinct from other preexisting clusters. New cluster 1 IBV strains represented recombinants between Korean nephropathogenic strain KM91 and the QXIBV strain. New cluster 2 IBV strains showed low amino acid homology (<58.7%) compared with previous isolates. We evaluated the protective efficacy of commercial IBV vaccines (H120 and K2 strain) against these new isolates. In cross-protection studies, the H120 strain did not provide sufficient protection against these variants. However, highly attenuated nephropathogenic IBV vaccine, K2 strain, provided significantly higher levels of protection against variants compared with chickens vaccinated with H120 (P < 0.05 or better). These results indicate that the K2 vaccine could be helpful for the reduction of economic losses caused by newly evolving IBV recombinants (new cluster 1) and variants (new cluster 2).</P>
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Lee, K.,Kim, S.‐,H.,Yoon, H. J.,Paik, D. J.,Kim, J. M.,Youn, J. Blackwell Publishing Ltd 2011 Clinical and experimental allergy Vol.41 No.8
<P><B>Summary</B></P><P><B>Background </B> Asthma is an inflammatory disease of the airways that is mediated by Th2 responses. Poly‐γ‐glutamic acid (γ‐PGA) is an extracellular polymeric compound that is synthesized by <I>Bacillus</I> cells. Previously, we found that γ‐PGA promoted Th1 cell development in a manner dependent on antigen‐presenting cells, but inhibited Th2 cell development.</P><P><B>Objective </B> To investigate the effect of γ‐PGA on dendritic cells (DCs), and its potential for treating Th2‐mediated allergic asthma.</P><P><B>Methods </B> Wild‐type, Toll‐like receptor (TLR)‐2 deficient, and TLR‐4‐defective mice were used. DCs derived from the bone marrow and extracted from the lung were stimulated with γ‐PGA and assayed for the expression of signalling molecules, costimulatory molecules, and cytokines. Mice were sensitized and challenged with ovalbumin (OVA) to induce asthma. They were repeatedly injected intranasally with γ‐PGA before and during the challenge period, and inflammation and structural remodelling of the airways were examined.</P><P><B>Results </B> γ‐PGA selectively signalled conventional DCs to activate NF‐κB and mitogen‐activated protein kinase, leading to the up‐regulation of CD86, CD40, and IL‐12, but not IL‐10 and IL‐6. These effects of γ‐PGA were dependent on TLR‐4 and independent of TLR‐2. Importantly, the intranasal administration of γ‐PGA to OVA‐sensitized/challenged mice reduced the airway hyperresponsiveness and allergic inflammation such as leucocyte influx, goblet cell hyperplasia, eosinophilia, and Th2 cytokine production. In addition to lowered IgE titres, the treatment of mice with γ‐PGA significantly reduced the multiplication and Th2 polarization of mediastinal lymph node T cells upon allergen‐specific restimulation. These anti‐asthmatic effects of γ‐PGA were also abolished in TLR‐4‐defective mice.</P><P><B>Conclusions and Clinical Relevance </B> Our data indicate that γ‐PGA activates DCs to favour Th1 cell induction through a TLR‐4‐dependent pathway and alleviates pathologic symptoms in a Th2‐biased asthmatic model. These findings highlight the potential of γ‐PGA for the treatment of asthma and other allergic disease in which Th2 polarization plays an important role.</P><P> <I>Cite this as</I>: K. Lee, S.‐H. Kim, H. J. Yoon, D. J. Paik, J. M. Kim and J. Youn, <I>Clinical & Experimental Allergy</I>, 2011 (41) 1143–1156.</P>
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Youn, S.,Jeong, S.,Kim, D.H. Elsevier Science Publishers 2014 CATALYSIS TODAY - Vol.232 No.-
In this study, we aimed at finding the optimum condition to prepare V<SUB>2</SUB>O<SUB>5</SUB>/TiO<SUB>2</SUB> catalyst for the selective catalytic reduction with NH<SUB>3</SUB> (NH<SUB>3</SUB>-SCR) by changing oxidation states in vanadium precursor solution. Using three precursor solutions with different oxidation states (V<SUP>3+</SUP>, V<SUP>4+</SUP> and V<SUP>5+</SUP>), TiO<SUB>2</SUB> supported V<SUB>2</SUB>O<SUB>5</SUB> catalysts (1, 3, 5 and 7wt%) were prepared by applying wet impregnation method. We utilized various analytical techniques, such as N<SUB>2</SUB> adsorption-desorption with BET method, inductively coupled plasma (ICP), X-ray diffraction (XRD), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), UV-visible diffuse reflectance spectroscopy (UV-Vis DRS) and temperature programmed reduction with hydrogen (H<SUB>2</SUB> TPR) to investigate the physicochemical properties of V<SUB>2</SUB>O<SUB>5</SUB>/TiO<SUB>2</SUB> catalysts. In addition, the SCR reactivity of these catalysts was evaluated to obtain a structure-activity relationship. It was found that V<SUB>2</SUB>O<SUB>5</SUB>/TiO<SUB>2</SUB> catalysts prepared from the precursor solution of V<SUP>3+</SUP> oxidation state (VT(3+)) has good activity over wide temperature range and selective to N<SUB>2</SUB> production, respectively. Also, they produced the least amount of N<SUB>2</SUB>O during SCR reaction, which is beneficial from the view point of greenhouse gas emission. The superiority of low temperature Such desirable reaction behavior of the VT(3+) catalyst is attributed to the formation of highly coordinated polymeric vanadyl species on the sample.
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