축구선수의 Detraining과 Retraining이 혈청지질 및 호르몬농도에 미치는 영향

정정화,박재현,채종훈,성혜련,황지인,윤미숙,노금선,윤종관,윤영학,노순덕,정경숙,박일규,김은희,박현태,박상갑 대한스포츠의학회 1999 대한스포츠의학회지 Vol.17 No.1

The purpose of this study was to investigate the effects of detraining and retraining on serum lipid and hormones in soccer players. Subjects were seven male high-school soccer players. V˙O_2max was determined for each subjects by administering a treadmill test(initial speed: 90m/min, grade: 5%, increasing speed per 3 min: 30m/min). Serum lipid(T-C, TG, HDL-C LDL-C) and hormones(epinephrine, norepinephrine, growth hormones, cortisol) were assayed pre and post detraining in 10, 20, 30 days after retraining. The repeated ANOVA was used to determine significant differences. The 0.05 level of significance was as critical level for the study. The results of the study were as follows: 1. V˙O_2max(ml/min) were 3576.3±204.2ml/min pre detraining, 3234.1±198.9 ml/min post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/min) were 3601.4±170.9 ml/min. There were significantly(p<.05) increased in retraining periods. 2. V˙O_2max(ml/kg/min) were significantly(p<.05) decreased from 62.3±2.9 ml/kg/min to 55.9±4.7 ml/kg/min in detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/kg/min) were 62.4±3.4ml/kg/min, 62.7±2.3ml.kg/min, 67.3±7.2ml/kg/min respectively. There were significantly(p<.05) increased in retraining periods. 3. T-C were significantly (p<.05) increased from 166.6±8.5mg/dl to 175.3±10.3 mg/dl in detraining. In 10, 20, 30 days after retraining, T-C were 160.1± 3.2mg/dl, 156.7±3.7mg/dl, 140.3±9.0mg/dl. There were significantly(p<.05) decreased in retraining periods. 4. HDL-C were 61.4±6.6mg/di pre detraining, 5.3±6.6mg/dl post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, HDL-C were 56.9±7.1mg/dl, 56.4±9.2mg/dl, 57.7±9.1mg/dl respectively. There were no significant difference in retraining periods. 5. The hormones(epinephrine. norepinephrine, growth hormone, cortisol) were changed as same patterns. Epinephrine were 26.0±7.0[g/ml pre detraining, 24.6±3.2pg/ml post detraining. In 10, 20, 30 days after retraining, epinephrine were 26.9±5.6pg/ml, 30.6±6.2pg/ml, 29.4±5.6pg/ml respectively. There were no significant difference in retraining periods. In conclusion, HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were decreased, T-C, LDL-C and TG were increased in detraining. But HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were increased, T-C, LDL-C and TG were decreased in retraining.

Thermomicrobium roseum 유래 Alcohol Dehydrogenase의 정제 및 특성

윤석영,홍민표,김은호,공광훈 중앙대학교 기초과학연구소 2000 基礎科學硏究所 論文集 Vol.14 No.-

Alcohol dehydrogenase from Thermomicrobium roseum was purified to electrophoretic homogeneity approximately 77-fold with a 38% activity yield by adenosine 5'-monophosphate and Mono Q column chromatography. The molecular weight of the enzyme was determined to be approximately 43,000 by SDS-polyacrylamide gel electrophoresis and 88,000 by gel chromatography, indicating a homodimeric structure. The Km and Vmax values of the enzyme for ethanol were 24.2 mM and 12.8 ㎛ol/min/mg, respectively. Pyrazole notably inhibited the enzymatic activity. Ca2+ ion activated the enzyme activity, but Cu2+ inhibited. The activity of the enzyme was optimal at pH 10.0 and 60℃.

고층 아파트 건물의 지진거동에 대한 물탱크의 영향 분석

윤석한,정은호,김희철 경희대학교 산학협력기술연구원 1998 산학협력기술연구논문집 Vol.4 No.-

Many structural aspects need to be considered to design the high-rise apartment building. The higher the building the larger the lateral loads such as in the case of wind and earthquake load due to the particular dynamic effects. The effect of the earthquake load is difficult to predict and differ to the structural characteristics of buildings although the shape of structures is similar, which is different from the case of a wind load. The heavy water tank on the apartment roof floors has a significant effect on the dynamic behaviours of buildings. The purpose of this study is to understand the behaviour of apartment building due to the weight of water tanks under the earthquake load. Dynamic analysis was conducted on two cases - 1) water tank is considered and 2) water tank is not considered - to analyse effect of water tank in a high-rise apartment building. Structural design was also considered to understand how the water tank on the roof floor affects each structural members.

말초혈액 단핵구와 단핵구 유래 세포주 THP1에서 FcrR 자극에 의해 유도되는 염증반응의 조절기전

윤강순,윤석란,이충은,김형순,변광호 大韓免疫學會 1993 大韓免疫學會誌 Vol.15 No.-

Regulation mechanisms of inflammatory responses induced by FcrR stimulation in human monocytes and monocytic cell line THP1 were investigated. Release of arachidonic acid, which is the precusor for inflammatory mediators, was induced by FcrR stimulation with its ligand human IgG or with anti FcrR mAbs. This response was further increased when FcrR was cross-linked with in-soluble anti-IgG-agarose and soluble anti-IgG Fc specific Fab fragments. These phenomena were shown in both monocyte and THP1. Oxidative burst activity, resulting from generation of reactive oxygen speices, was also induced by FcrR stimulation and further enhanced by cross-linking of Fcr R. Induction mechanisms of inflammatory responses caused by PMA or FcrR stimulations were then examined by measurements of release of arachidonic acid and oxidative burst activity. The results demonstrate that monocytes utilize different signaling pathways for FcrR or PMA stimulation. Signaling mechanism of PMA stimulation is especially dependent on Ca}+/calmodulin dependent kinase in arachidonic acid release, but not in oxidative burst activity. Meanwhile, signaling pathways of FcrR are dependent on tyrosine kinase but independent of protein kinase C both in the release of arachidonic acid and oxidative burst activity. The results suggest that in monocytes, there is a not only functional but also mechanistic link between arachidonic acid release and oxidative burst activity induced upon FcrR stimulation. Distinct signaling pathways seem to be operating in transformed monocytic cell line THP-1 cells, and no mechanistic link was found between arachidonic acid release and oxidative burst activity induced after FcrR stimulation.

B 림프구 활성화 자극에 따른 Interleukin-4 수용체와 Type II IgE 수용체 발현조절양상의 상호관련성

이충은,윤석란,변광호,소의영 大韓免疫學會 1995 大韓免疫學會誌 Vol.17 No.3

Interleukin-4(IL-4) specifically induces the expression of its own receptor, IL-4R and type II IgE receptor, FceR1l in a dose-dependent manner in human B lymphocytes. We observed that the IL-4-induced expression of IL-4R and Fce Rll were both down-regulated by interferon-T(IFN-T) at mRNA as well as surface protein levels in normal B lymphocytes. Such co-ordinated regulation of the IL-4-induced IL-4R and FceRlI also occurred by anti-CD40 and steroid, each regulating the IL-4-induced response in an opposite direction. While anti-CD40, which is re-ported to agument the IL-4-induced B cell activation, further enhanced the IL-4-induced IL-4R and Fee RU expression, methylprednisolon(MPD) strongly inhibited the level of these receptors. The anti-CD40 or MPD-induced modulation of the IL-4-induced IL-4R and FceRII was further subject to the down-regulation by lFN-T. As compared to normal B lymphocytes, a differential regulation of IL-4R and FceRII was noted in a transformed B cell line, where IFN- Tup-regulated the IL-4-induced responses. Still, there was a co-ordinated regulation of the IL-4R and FceRII in these cells. Taken together, these results suggest that modulation of IL-4R expression and FccRII induction are closely correlated events during the IL-4-induced cellular activation

노인의 갑상선기능저하증에 대한 임상적 연구

정윤석,남문석,이은직,김경래,이현철,김현승,허갑범 한국노화학회 1993 한국노화학회지 Vol.3 No.1

복합트레이닝이 수영경기력 및 근력에 미치는 영향

이정숙,안민호,이은희,신옥연,김영준,이윤관 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.6

This study was aimed to find what impacts the combined training of circuit weight training and swimming training can give to the competing power of swimming athletes and their respiratory functions and to provide the basic data for preparing the desirable training program to shorten swimming records through 14 girl swimming athletes, a group of 7 athletes in circuit weight training and another 7 in swimming training and the following conclusions were obtained: 1. Swimming and Circuit training groups showed, before and after 12 week training, the changes of muscular strength: grasping power (p<.001), back muscle (p<.01), arm extensor (p<.05), leg extensor (p<.05), leg curl extensor (p<.01), broad jump (p<.05) increased espectively than those of swimming training group and which were more effective. 2. In the comparison between groups, the meaningful difference appeared in swimming training groups, showing grasping power (p<.01), back muscular strength (p<.01), arm extensor (p<.05), leg extensor (p<.01), leg extensor (p <.01) 3. In the changes of competition record, in swimming and circuit training group, before and after 12 week training, meaningful increases appeared by 50m record(p<.05), 200m record (p<.05) 4. In the changes of competition record, as for the comparison between groups, meaning ful increases, 50m record (p<.05), 200m record (p<.05) appeared.

영어 사역화 동사의 의미자질

장은숙,윤웅진 신한영미어문학회 1997 새한영어영문학 Vol.36 No.-

The purpose of this study is to examine the event structure expressed by causative verbs and the features determined by thematic roles in causative sentences, and to testify semantic features causing causativization. It argues that the vocabulary basically included many kinds of conceptual constituents (EVENT, STATE, THING, PLACE, PATH, MANNER, etc), features defining predicates for kinds of events/state, place functions(at, in, on, around, over, under, near), and path functions(to, from, toward, away-from). The event structure considering conceptual constituents and features can explain relationship between causative sentences and thematic roles. In this study, the causativization process must not be expressed by syntactic transformation limited to verbs, but by aktionsart as well as properties of noun phrases and adverbial phrases.

정형외과 환자의 관여도에 따른 병원급식 만족도 분석

심은영,윤석권,홍완수 대한영양사협회 2004 대한영양사협회 학술지 Vol.10 No.2

The purposes of this study were to evaluate the quality of hospital food services in view of patients in orthopedic wards and accomplish the quality improvement in hospital foodservice operations. Quantitative questionnaires for patients containing foodservice satisfaction and demographic information were developed. A survey of 8 general hospitals was undertaken and detailed information was collected from 290 patients in orthopedic wards. The collected data were processed using the SAS PC 6.12 for descriptive analysis and t-test. In demographic information of patients, 32.6% was over 50 years old and 3 1% was hospitalized over 30 days. 80% of patients was taking normal diet. 47.7% and 47.9% of patients showed moderate appetite and moderate pain respectively. The overall satisfaction score for patients was 3.24 out of 5, showing slightly higher level than the average score(3.00). According to foodservice involvement scores of patients, they were divided into two groups which were high involved group and low involved group. Two groups showed significant differences in taste of meals, variety of menu, punctuality of meal times, temperature of meals and portion size. The foodservice involvement factor which affected significantly patient foodservice satisfaction was 'kindness of foodservice staff'.

인체 B 림프구 활성화의 신호전달과정에서 IL-4 수용체와 CD40 분자간의 상호작용

이충은,윤석란,변광호 大韓免疫學會 1995 大韓免疫學會誌 Vol.17 No.3

Stimulation of B cells through CD40 antigen has been reported to agument the interleukin-4(IL-4)induced B cell proliferation and differentiation. In order to elucidate mechanisms underlying the synergistic action, we examined the effect of CD40-mediated signal on the IL-4-induced B cell activation. Treatment of dense, resting tonsillar B cells with agonistic anti-CD40 mAb caused a noticeable induction of IL-4 receptor(IL-4R) mRNA expression within hours, which was followed by a significant enhancement of the IL-4-induced surface IL-4R and CD23 expression, suggesting a role of CD40 in amplifying the IL-4-induced signal. Signal interplay between the IL-4 and the CD40 system was further supported by the observation that, in tonsillar B cells, IL-4 plus anti-IgM induced CD40 expression and tyrosine phosphorylation of IL-4R, and that anti-CD40 co-immunoprecipitated the IL-4R and the IL-4R associated tyrosine kinase activity, which implies a possible molecular interaction between IL-4R and CD40. Together these results suggest that there is a cross-talk between CD40-and the IL-4R-mediated signal transduction system during the T cell-dependent B cell activation.