Pollen Viability of Common Buckwheat (Fagopyrum esculentum Moench.) in in vitro Germination and its Application to Male Gametophyte Isolation

Woo, Sun-HeeㆍKeun-Yook ChungㆍHeung-Tae, KimㆍYong-Gu, ChoㆍHong-Sig, KimㆍBeom-Heon, Songㆍ Chul-Won, Lee and Seung-Keun Jong 忠北大學校 農業科學硏究所 2004 農業科學硏究 Vol.21 No.-

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석탄 가스화를 통한 전력 생산과 DME 병산 공정에 대한 기초 경제성 분석

유영돈 ( Young Don Yoo ),김수현 ( Su Hyun Kim ),조원준 ( Won Jun Cho ),모용기 ( Yong Gi Mo ),송용택 ( Tae Kyong Song ) 한국화학공학회 2014 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.52 No.6

석탄가스화를 기반으로 한 발전(IGCC 발전) 및 화학원료 제조공정의 상업화 관건은 화석연료인 원유 또는 천연가스를 기반으로 생산되는 경우와 비교하여 경제성을 확보할 수 있는지 여부이다. 경제성 확보를 위한 가장 현실적인 방법으로는 석탄 가스화를 통해 얻어진 합성가스로부터 2개 이상의 생산물(예: 발전과 화학원료를 동시 생산)을 병산(coproduction또는 poly-generation)하는 것이다. 본 연구에서는 석탄 가스화를 기반으로 하여 발전과 수송용, 발전용 및 가정용 연료로 사용이 가능한 DME(dimethyl ether)를 병산하는 공정에 대한 경제성 분석을 실시하였다. 경제성 분석을 위한 병산 공정에서는 250 MW 전력생산 연간 30만 톤의 DMZ 생산을 기준으로 하였다. 병산 공정에서 DME 판매가격이 50만원/ton인 경우, 전기 생산원가는 34.8~58.4원/kWh으로 SMP(계통한계가격) 가중평균인 150.69원/kwh(2013년 1월~12월까지의 평균값)의 33~58% 수준으로 산정되었다. 따라서, DME 판매가격이 적정하게 유지될 경우 석탄IGCC+DME 병산공정은 IGCC 단독 발전과 비교하여 경제성을 확보할 수 있을 것으로 판단된다. 현재 중국에서 DME판매가격이 900,000원/톤 내외이므로, 전력과 DME를 병산할 경우, IGCC 단독으로 전력을 생산할 경우와 비교하여 전력 생산 원가를 월등하게 낮출 수 있음을 알 수 있다. 이와 같이 석탄 가스화를 기반으로 한 병산 공정을 통해 전력과 DME를 병산하는 시스템에서, 시장 여건에 따라 전력과 DME 생산비율 제어가 가능하고, 석탄 가스화기 및 정제 시스템을 공통 설비로 활용함으로써, 개별적으로 생산하는 것보다 생산 원가를 낮출 수 있다는 결과를 얻었다. The key for the commercial deployment of IGCC power plants or chemical (methanol, dimethyl ether, etc.) production plants based on coal gasification is their economic advantage over plants producing electricity or chemicals from crude oil or natural gas. The better economy of coal gasification based plants can be obtained by co-production of electricity and chemicals. In this study, we carried out the economic feasibility analysis on the process of co-producing electricity and DME (dimethyl ether) using coal gasification. The plant’s capacity was 250 MW electric and DME production of 300,000 ton per year. Assuming that the sales price of DME is 500,000 won/ton, the production cost of electricity is in the range of 33~58% of 150.69 won/kwh which is the average of SMP (system marginal price) in 2013, Korea. At present, the sales price of DME in China is approximately 900,000 won/ton. Therefore, there are more potential for lowering the price of co-produced electricity when comparing that from IGCC only. Since the co-production system can not only use the coal gasifier and the gas purification process as a common facility but also can control production rates of electricity and DME depending on the market demand, the production cost of electricity and DME can be significantly reduced compared to the process of producing electricity or DME separately.

A SPATIO-TEMPORAL RECEIVER SLOWLY-VARYING WIRELESS CDMA CHANNEL

Yong Song(송용),Jong Gil Nam(남종길),Won Cheol Lee(이원철) 한국통신학회 1998 한국통신학회 학술대회논문집 Vol.1998 No.11

A New Black Seed Coat Soybean Cultivar, "Cheongyeob 1" with Wrapping in Soybean Leaves

Won Young Han,In Youl Baek,Hyun Tae Kim,Jong Min Ko,Myung Gi Jeon,Keum Yong Park,Byung Won Lee,Young Hoon Lee,Sang Ouk Shin,Sea Gyu Lim,Min Jung Soe,Yong Duck Kim,In Kwan Song 한국육종학회 2011 한국육종학회 학술발표회 발표요지 Vol.43 No.-

Cloning and Characterization of UV-B Inducible Chalcone Synthase from Grape Cell Suspension Culture System and Its Expression Compared with Stilbene Synthase

Song, Won-Yong,In, Jun-Gyo,Lim, Yong-Pyo,Park, Kwan-Sam Korean Society of Potoscience 2000 Journal of Photosciences Vol.7 No.2

We performed the cloning of a chalcone synthase (CHS) gene, the key enzyme in the anthocyanin biosynthesis, from the cDNA library constructed with grape suspension cells irradiated UV-B. The PCR fragment was used to cloning the CHS gene. One CHS cDNA clone containing an open reading frame and a partial stilbene synthase (STS)cDNA, the stilbene-type phytoalexin, were isolated. The CHS cDNA clone (VCHS) showed 87% sequence homology with VvCHS (V.vinifea) and 72.3% identity with VSTSY(V.vinifea). its amino acid sequences were longer than any other CHS genes as 454 residues. Two genes were weakly expressed in white light irradiated cells, but highly induced in UV-B irradiated condition during 32 hours. Interestingly, the STS was quickly and abundantly expressed from 2 hours when supplemented with jasmonic acid (JA) and the maximum expression was observed at 4 hours and then gradually decreased. But, the additional UV-B or white light quickly degraded the STS expression than only JA treated grape suspension cells. The CHS also was rapidly induced with JA and the synergistical effect was observed at the addigional light treatment of UV-B or white light. These results are indicated that CHS and STS have different response mechanisms against the environmental stresses.

Management of Regional Lymph Nodes in Localized Vulvar Carcinoma

Won Il Jang(장원일),Hong-Gyun Wu(우홍균),Charn Il Park(박찬일),Sung Whan Ha(하성환),Hyo Pyo Lee(이효표),Soon Beom Kang(강순범),Yong Sang Song(송용상) 대한방사선종양학회 2008 Radiation Oncology Journal Vol.26 No.1

목 적: 외음부 암환자에서 국소영역 치료실패에 대한 수술 후 방사선치료의 영향을 평가하고 임상적으로 림프절이 전 이가 없는 환자들에서 서혜부 림프절에 대한 치료방침을 결정해 보고자 하였다. 대상 및 방법: 1979년 10월부터 2004년 6월까지 서울대학교병원에서 일차성 외음부 암으로 치료를 받은 환자 66명에 대해 후향적 분석을 시행하였다. 이들 중에서 원격전이가 있는 2명, 고식적 목적으로 치료를 받은 6명, 이전에 골반부위 방사선 치료의 병력이 있는 3명, 추적관찰이 탈락된 4명, 의무기록이 불충분한 1명을 포함하여 16명의 환자들은 이번 분석에서 제외되었다. 50 명 중에 수술만 받은 환자가 35명, 수술과 방사선 치료를 받은 환자가 10명, 방사선 치료만을 받은 환자가 5명이었다. 결 과: 5년 전체 생존율과 무병 생존율은 각각 91%, 78%였다. 12명(26%)에서 치료 실패를 보였으며, 국소 실패가 8명, 영역림프절 전이가 3명, 원격 전이가 1명이었다. 수술과 방사선치료를 같이 받은 환자들이 수술만을 받은 환자들보다 위험요인을 더 많이 가지고 있었지만, 무병 생존율은 두 집단에서 통계적으로 유의한 차이를 보이지 않았다(5년무병 생존율 78% vs. 83%, p=0.66). 잠재성 림프절 전이의 빈도는 10%였다. 임상적으로 림프절 전이가 없었던 31명의 환자들 중에서 10명은 서혜부 림프절 절제술을 받지 않았지만, 이들 중에서 영역림프절 전이를 경험한 사람은 아무도 없었다. 결 론: 치료실패의 위험요인을 가진 외음부 암자들에게 수술 후 방사선 치료는 잠재적인 이점을 가지고 있다. 임상적으로 림프절 전이가 없는 위험도가 낮은 환자들에게는 서혜부 림프절 절제술을 하지 않는 것이나 서혜부 림프절에 대해 예방적 방사선치료를 하는 것에 대해서 고려해 볼 수 있겠다. Purpose: To evaluate the impact of postoperative radiotherapy on loco-regional failure in patients with vulvar carcinoma and to determine the treatment strategy for inguinal lymph nodes. Materials and Methods: Sixty-six patients who received treatment for primary vulvar carcinoma at Seoul National University Hospital, from October 1979 through June 2004, were retrospectively analyzed. Sixteen patients were excluded from the analysis due to the following reasons: distant metastases in two patients; palliative intent for six patients; previous radiotherapy given to the pelvis in three patients; follow-up loss after surgery for four patient; insufficient medical records for one patient. Of 50 eligible patients, 35 were treated with surgery alone (S), ten were treated with surgery followed by radiotherapy (S+RT), and five were treated with radiotherapy alone. Results: The 5-year overall survival (OS) and disease-free survival (DFS) rates of all patients were 91% and 78%, respectively. Twelve patients (26%) experienced treatment failures and the sites of initial failure were as follows: a primary site in eight patients; regional lymph nodes in three patients; the lung in one patient. Although risk factors for failure were more common in the S+RT group than the S group of patients (p <0.05), the DFS rates were similar for the two groups (5-year DFS rates, 78% vs. 83%, p=0.66). The incidences of occult lymph node metastases was 10%. Ten of 31 patients with clinically negative lymph nodes did not received inguinal lymph node dissection, but no patient experienced regional failure. Conclusion: Postoperative radiotherapy may have a potential benefit for patients with risk factors for failure. The omission of inguinal dissection or elective radiotherapy to the inguinal lymph nodes may be considered in low-risk patients with clinically negative lymph nodes.

Purification and Characterization of a Serine Proteinase from Acanthamoeba culbertsoni

Song, Chul Yong,Park, Ki Won 생화학분자생물학회 1982 BMB Reports Vol.29 No.5

A serine proteinase was purified from Acanthamoeba culbertsoni by 41∼80 ammonium sulfate fractionation, ion exchange chromatography, affinity chromatography and gel filtration chromatography. The molecular weight of the purified enzyme was estimated to be 108.0 kDa by gel filtration chromatography and 54.0 kDa by SDS-PAGE_ Therefore, the purified enzyme seemed to be a dimer. Isoelectric point was 4.5. The enzyme activity was highly inhibited by the serine proteinase inhibitors diisopropyl fluorophosphate (DFP) and phenylmethyl sulfonylfluoride (PMSF). It had a narrow pH optimum of 6.5∼7.5 with a maximum at pH 7.0. These data suggested that the purified enzyme was a neutral serine proteinase. Optimal temperature was 37℃. It was stable for at least 16 h at 4℃ and 37℃, but it was rapidly inactivated at 65℃. The activity of the purified enzyme was not influenced significantly by Mg^(2+). Mn^(2+). Zn^(2+) or Ca^(2+). However, the enzyme activity was highly inhibited by Hg^(2+). The enzyme degraded type I collagen and fibronectin, but not BSA, hemoglobin, lysozyme, immunoglobulin A or immunoglobulin G.

A Flucytosine-Responsive Mbp1/Swi4-Like Protein, Mbs1, Plays Pleiotropic Roles in Antifungal Drug Resistance, Stress Response, and Virulence of Cryptococcus neoformans

Song, Min-Hee,Lee, Jang-Won,Kim, Min Su,Yoon, Ja-Kyung,White, Theodore C.,Floyd, Anna,Heitman, Joseph,Strain, Anna K.,Nielsen, Judith N.,Nielsen, Kirsten,Bahn, Yong-Sun American Society for Microbiology 2012 EUKARYOTIC CELL Vol.11 No.1

<B>ABSTRACT</B><P> Cryptococcosis, caused by the basidiomycetous fungus Cryptococcus neoformans , is responsible for more than 600,000 deaths annually in AIDS patients. Flucytosine is one of the most commonly used antifungal drugs for its treatment, but its resistance and regulatory mechanisms have never been investigated at the genome scale in C. neoformans . In the present study, we performed comparative transcriptome analysis by employing two-component system mutants ( <I>tco1</I> Δ and <I>tco2</I> Δ) exhibiting opposing flucytosine susceptibility. As a result, a total of 177 flucytosine-responsive genes were identified, and many of them were found to be regulated by Tco1 or Tco2. Among these, we discovered an APSES-like transcription factor, Mbs1 ( Mb p1- and S wi4-like protein 1). Expression analysis revealed that <I>MBS1</I> was regulated in response to flucytosine in a Tco2/Hog1-dependent manner. Supporting this, C. neoformans with the deletion of <I>MBS1</I> exhibited increased susceptibility to flucytosine. Intriguingly, Mbs1 played pleiotropic roles in diverse cellular processes of C. neoformans . Mbs1 positively regulated ergosterol biosynthesis and thereby affected polyene and azole drug susceptibility. Mbs1 was also involved in genotoxic and oxidative stress responses. Furthermore, Mbs1 promoted production of melanin and capsule and thereby was required for full virulence of C. neoformans . In conclusion, Mbs1 is considered to be a novel antifungal therapeutic target for treatment of cryptococcosis. </P>

Conjugated Polymer Consisting of Quinacridone and Benzothiadiazole as Donor Materials for Organic Photovoltaics: Coplanar Property of Polymer Backbone

Song, Ho-Jun,Kim, Doo-Hun,Lee, Eui-Jin,Heo, Soo-Won,Lee, Jang-Yong,Moon, Doo-Kyung American Chemical Society 2012 Macromolecules Vol.45 No.19

<P>Highly soluble poly[quinacridone-<I>alt</I>-benzothiadiazole] (PQCDTB) has been synthesized through the Suzuki coupling reaction by introducing planar quinacridone and highly absorbing benzothiadiazole. The PQCDTB dissolved in general organic solvents, and the <I>M</I><SUB>n</SUB> was 49.8 kg/mol. The optical band gap energy was 1.92 eV, which was similar to the band gap of benzothiadiazole. The HOMO and LUMO levels of PQCDTB were −5.24 eV and −3.32 eV, respectively. According to the XRD measurement, PQCDTB showed the formation of ordered lamellar structure as an out-of-plane peak (<I>h</I>00) by the alkyl side chain of quinacridone and conventional edge-on π- stacking. This study also evaluated the OPV characteristics by fabricating a bulk-heterojunction–type polymer solar cell. When PQCDTB and PC<SUB>71</SUB>BM were fabricated in a 1:1 weight ratio, the open-circuit voltage (<I>V</I><SUB>OC</SUB>), short-circuit current (<I>J</I><SUB>SC</SUB>), fill factor (FF), and power conversion efficiency (PCE) were 0.79 V, 5.6 mA cm<SUP>–2</SUP>, 55.8% and 2.5%, respectively. In particular, PQCDTB exhibited higher FFs compared with poly[carbazole-<I>alt</I>-benzothiadiazole] (PCZDTB).</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/mamobx/2012/mamobx.2012.45.issue-19/ma301466m/production/images/medium/ma-2012-01466m_0009.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ma301466m'>ACS Electronic Supporting Info</A></P>

Characterization of the skin mycobiome in patients with atopic dermatitis

( Song Hee Han ),( Joo Ran Hong ),( Hye In Cheon ),( Min Seok Hur ),( Byung Gon Choi ),( Min Jung Kim ),( Hae Jeong Youn ),( Yang Won Lee ),( Yong Beom Choe ),( Kyu Joong Ahn ) 대한피부과학회 2017 대한피부과학회 학술발표대회집 Vol.69 No.2

Background: With the recent availability of culture-independent sequencing methods, studies have been conducted to analyze skin microorganisms present in patients with atopic dermatitis (AD). However, the database on the skin fungal communities, “mycobiome,” has been relatively restrictive compared with the bacterial world. Objectives: This study aimed to comparatively analyze the overall skin mycobiome between patients with AD and healthy individuals in the Korean population. Methods: Skin swab samples from the antecubital fossae of 8 patients with AD and 8 healthy controls were analyzed. Using sequencing method followed by direct DNA extraction and molecular PCR, taxonomic compositions of fungi at stepwise level ranks were analyzed. Results: The phylogenic marker used was internal transcribed spacer 2 regions of DNA. The tendency of higher intra- and inter-personal taxonomic diversity at genus and species levels in AD samples was observed. Non-Malassezia fungal diversity was also noticeable in the patient group compared with healthy controls. Malassezia globosa and Malassezia restricta were prevalent in all samples across both study groups, and some Malassezia species, including Malassezia sloofiae and dermatis characterized AD. Conclusion: This study might provide a new insight into the mycobiome of adult AD, which contributes to building a systemic mycobiome database in AD.