정상 한국인에서 연령증가에 따른 혈장 Dehydroepiandrosteronesulfate ( DHEA-S ) 농도 및 DHEA - S / cortisol 비의 변화

박성우,유형준,임성희,최문기,유재명,박민숙,이권엽,박철수,김철홍,김현규,김두만,박규용 대한내분비학회 1997 Endocrinology and metabolism Vol.12 No.2

Backgroud: DHEA-S is the most abundant steroid hormone in circulation, and primarily secreted from the adrenal cortex, but its physiological role is little known. One of the characteristic features of DHEA-S is progressive decrement of plasma DHEA-S level with advancing age, in contrast, plasma levels of other adrenal hormones are not chaging or littie decreasing. To grasp the trends of plasma DHEA-S level and DHEA-S/cortisol ratio by age in healthy Korean, we measured the plasma DHEA-S levels and DHEA-S/cortisol ratios in healthy Korean. Methods: Healthy Korean(men: 99, women: 102, age range: 15-97 year old)were studied. Subjects were not taking drugs(such as glucocorticoid or androgenic medication) or cigarettes known to modify the plasma level of DHEA-S and cortisol, and had no evidence of hepatic, renal disease or hyperlipidemia as determined by serum lipid, bilirubin, SGOT, SGPT, BUN, creatinine. Data were analyzed by 10-year age group for men and women: i.e, 10-19, 20-29, 30-39, 40- 49, 50-59, 60-69, 70-79, 80-89 and 90 year or more. Plasma DHEA-S levels were measured by using a commercially available RIA kit with 125I labeled-DHEA-SO4(Coat-A Count DHEA-SO4), and for the measurement of plasma cortisol levels, commercial Gamma Coat TM[125I] Cortisol Radioimmunassay Kit was used. Results:. 1) In both men and women, plasma DHEA-S level showed high interindividual variation within the same age group. 2) There were individual sex differences in plasma levels of DHEA-S, in all age groups, plasma DHEA-S levels were significantly higher values for men than for women. 3) Maximum plasma DHEA-S levels(men; 237+-3.35 ug/dL, women; 108+-17.5 ug/dL) were at third decade in both men and women. 4) Both men and women showed the continuous decline in plasma DHEA-S level with age. These age-related decline was more prominent in men than in women(men; y= -3.152 * +292.6, r2= 0.8459, P$lt;0.05, women; y= -1.417 * +143.3, r2 = 0.7278, P$lt; 0.05). 5) As an index of aging, there was no stastical difference between DHEA-S and DHEA- S/cortisol ratio. Conclusion: In healthy Korean, there were high interindividual variation of plasrna DHEA-S levels. In both men and women plasma DHEA-S level was peak at third decade, and from when it declined progressively with age. These results suggest that although the reliability of single plasma DHEA-S measurement are limited, the decline of DHEA-S with advancing age might be a specific marker of endocrinologic hormonal milieu(aging index). Also, concerning to individual adrenal secreting capacity, we measured DHEA-S/cortisol ratio. But we did not found that plasma DHEA-S/cortisol ratio is superior to the plasma DHEA-S level as an aging index. (J Kor Soc Endocrinol 12:245-254, 1997)

폴록사머를 이용한 디클로페낙 고형 좌제의 개발

용철순,오유경,김정애,김용일,박상만,양준호,이종달,최한곤 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.2

To develop a poloxamer-based solid suppository with poloxamer mixtures, the melting points of various formulations composed of P 124 and P 188 were investigated. To investigate the effect of poloxamer to the dissolution and dissolution mechanism of diclofenac sodium from the suppository the dissolution of diclofenac sodium delivered by the poloxamer-based suppository was performed. Furthermore, to investigate the mucoadhesive property of the poloxamer-based solid suppository, the identification test in the rectum was carried out after its rectal administration in rats. The poloxamer mixtures composed of P 124 and P 188 were homogeneous. Very small amounts of P 188 affected the melting points of poloxamer mixtures. In particular, the poloxamer mixture [P 124/P 188 (97/3%)] with the melting point of about 32℃ was a solid form at room temperature and instantly melted at physiological temperature. Furthermore, very small amounts of P 188 in the poloxamer-based suppository hardly affected the dissolution rates of diclofenac sodium from the suppository. Dissolution mechanism analysis showed the dissolution of diclofenac sodium was proportional to the time. At 4 h after administration, the blue color of poloxamer-based suppository [diclofenac sodium/poloxamer mixture (2.5/97.5%)] with the P 124/P 188 ratio of (97/3%) and blue lake in the rectum was faded. However, the position of suppository in the rectum did not significantly change with time. Thus, it retained in the rectum for at least 4 h. Our results indicated that the poloxamer-based solid suppository with P 124 and P 188 would be a candidate of rectal dosage from for diclofenac sodium.

아세클로페낙 연질캡슬(클란자 에스 연질캡슬)의 개발

용철순,이경희,최진석,박병주,정세현,김용일,박상만,배명수,김귀자,김영식,유창훈,강성룡,유봉규,이종달,최한곤 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.1

To develop and aceclofenac soft capsule, four preparations with various solubilizers were prepared and their dissolution test was carried out. Among four preparations tested, a preparation with ethanolamine was selected a formula of aceclofenac soft capsule (Clanza S^(™), since it showed the fastest dissolution rate. Bioequivalence of aceclofenac tablet, Airtal^(™)(Dae-Woong Pharmaceutical Co., Ltd.) and aceclofenac soft capsule, Clanza S^(™)(Korea United Pharmaceutical Co., Ltd.) was evaluated according to the guideline of KFDA. Fourteen normal male volunteers (age 20-25 years old) were divided into two groups and a randomized 2×2 cross-over study was employed. After oral administration of one tablet or capsule containing 100 ㎎ of aceclofenac, blood ws taken at predetermined time intervals and the concentration of aceclofenac in plasma wa determined with an HPLC method under UV detector. The pharmacokinetic parameters (C_(max), T_(max) and AUC_(t)) were calculated and ANOVA was utilized for the statistical analysis of parameters using logarithmically transformed AUC_(t), C_(max) and T_(max) between Airtal tablet and Clanza soft capsule were 2.89%, 0.18% and 43.0%, respectively. There were no sequence effects between two formulations in these parameters. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of log(0.8) to log(1.250(e.g.log(0.81) - log(1.23) and log(0.89) - log(1.14)) for AUC_(t) and C_(max), respectively. Thus, the criteria of the KDFA guidelines for the equivalence was satisfied, indicating that Clanza S^(™) soft capsule is bioequivalent to Airtal^(™) tablet.

당뇨병 환자의 사례관리 실시 전후 혈당 및 자가관리 이행수준 변화

최용우,이용환,전만중,유병철 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Background : Diabetes mellitus is one of the common chronic disease in Korea. As most chronic diseases need continuous cares and behavioral controls, one of the effective managements for diabetes is maintenance healthy life style which is based on correct medical knowledge. So, we conducted this study to assess change of blood sugar levels and self-management behavior of diabetic patients following the case-management program enforced by National Health Insurance Corporation (NHIC). Methods : We conducted this study during the period from January 2006 to December 2006, 29 case managers performed the case management program for 672 diabetic patients in the districts of Busan, Woolsan, and Gyeongnam. We evaluated the effectiveness of the case management program based on the changes of blood sugar level, knowledge for diabetes and self-management behavior of diabetic patients. Data analysis was performed with t-test, paired t-test, McNemar X2-test and correlation analysis using SPSS program(ver 12.0). Results : After the case management program, smoking rate was reduced from 22.3% to 21.4% and exercise rate was increased from 65.0% to 80.2%(p<0.05). The knowledge standard of diabetics on 10-point scale revealed considerable increase from 6.05 before the management to 8.69 after the case management(p<0.05). All of diabetic self-management behaviors including nutrition management, self-test on blood sugar level, foot care, oral care and diabetic medication showed considerable improvement; the overall behavioral change, on a 5-point scale, improved from 3.25 to 3.75(p<0.05). The FBS level was improved from 138.4mg/dl to 131.5mg/dl and the blood sugar level 2 hours after a meal was improved from 203.7mg/dl to 183.5mg/dl(p<0.05). Conclusion : From the above results, we concluded that the case management program enforced by the NHIC showed positive behavioral changes on smoking and exercise, increased knowledge about diabetes as well as the level of nutrition management and also demonstrated decline of blood sugar levels. So, we need research of customized information for every patients rather than generally summarized information on diabetes and a self-dependent group formations for sustaining improved self-management after case management as well as development of education and training programs for case managers.

CGI 프로그램에서 클라이언트와 서버의 자동화된 상태정보 전달

최준용,유진호,장종표,방만원 木浦大學校 情報産業硏究所 1997 情報産業硏究誌 Vol.5 No.-

The HTTP Protocol composed of WWW's client/server model is unconnected and not keep state, so, there is one connection for one request. In this condition, it has no guarantee to memory which is needed in sequential processing, and HTTP Protocol has no choices to transfer the information from client to server's CGI or the information exchange. This paper uses the word 'key' instead of this 'state information'. We studied the key which creates automatically the HTML document for data search hierarchically in WWW environment and to confirm applied this to multimedia order and sale system of agricultural and marine products.

생쥐 2-세포기 수정란의 초급속동결

강만종,이철상,한용만,유대열,이경광 제주대학교 농과대학 제주도축산문제연구소 1991 畜産論叢 Vol.6 No.1

This study was carried out in order to investigate eggects of cryoprotectant concentration and equilibration time on survival of ultrarapidly frozen 2-cell mouse embryos Mouse 2-cell embryos. fol-lowing dehydration by exposure to DMSO and sucrose. were directly immersed into liquid nitrogen and thawed in 37℃ water. Viability was defined by development rate to the blastocyst stage after in vitro culture for 72 hours. The results are summarized as follows ; 1. When 0.25M of sucrose was added into the freezing medium a t various concentrations of DMSO and dilution medium, higher development rate of embryo was obtained in 3.0M DMSO conentrations (82.6%). However, When sucrose concentrations of 0.25 and 0.5M were added to the freezing medium with 3.0M DMSO and dilution medium, development rate of embryos were 81.7% and 24.1%, respectively. 2. In the equilibration time at room temperature, higher development rate was attained after short period of time(2.5min) in 3.0M DMSO +0.25M sucrose(85.9%). 3. The development rate of embryos at in vitro 2-celL in vivo 2-celL solution control and untreated control was 84.6%, 90.9%, 89.9%. and 89.7%. respectively.

부산지역 일부 종합병원의 컴퓨터 단말기 작업을 하는 여성 원무업무종사자들에 있어서 누적외상성장애에 대한 조사

전만중,유병철,고광욱,이용환 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

registration system were organized, computerized, and simplified. Especially the workers for the hospital registration desk were almost used computer all the working time. Therefore we assessed cumulative trauma disorder of registration desk workers related to computer use of general hospital. Methods: 62 women computer users who worked for registration desk of general hospitals in Busan were assessed from March to August, 2007. The workers were checked general characteristics(age, educational level, and marital status), work related characteristics, and symptoms by self-administered questionnaires. Results: Significantly decreased prevalences for cumulative trauma disorder were observed for subjects who had more educational level, the prevalence of cumulative trauma disorder was 69.2% among high school graduates, 38.2% among junior college graduates, and 20.0% among college graduates. Significantly increased prevalences for cumulative trauma disorder were observed for subjects who had more duration of computer use, the prevalence of cumulative trauma disorder was 56.5% for those who had used computer for more than 8 hours, 32.1% for those who had used computer for 4-7 hours, and 27.3% for those who had used computer for less than 3 hours. And Significantly increased prevalences for cumulative trauma disorder were observed for subjects who had more maximun daily computer use time without break, the prevalence of cumulative trauma disorder was 66.7% for those who had used computer for more than 7 hours, 38.1% for those who had used computer for 3-6 hours, and 21.7% for those who had used computer for less than 2 hours. Multiple logistic regression showed that subjects who had more maximum daily computer use time without break was more likely to report patient. Conclusion: Therefore these findings suggest that preventive interventions on hospital registration desk worker related to computer use were needed

저소득 독거노인의 생활만족도 관련요인

강지연,전만중,유병철,이용환 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.4

Background : To access the influential factors of life satisfaction in low-income and living alone old people for solving the problem of the estranged elderly. Method : We conducted a questionnaire survey during the period from June 2007 till September 2007. Investigation subjects were 152 elderly people registered in two welfare centers in Busan. We measured life satisfaction of low-income and living alone old people and analyzed with descriptive analysis, t-test, one-way ANOVA and dummy variable regression analysis using SPSS Win(ver 12.0k) program. Result : The mean score of life satisfaction in low-income and living alone old people was 2.52 ± 0.56. Life satisfaction in low-income and living alone old people was relatively high in groups of 65-69 years old, elementary school graduates, subjectively healthy, exercise, hobby or leasure activity, regular repast, 3 times repast per day and income satisfaction. Life satisfaction in low-income and living alone old people was influenced by age, religion, satisfaction of income, subjective health conditions and regular repast. Conclusion : From the result, we can concluded that life satisfaction in low-income and living alone old people was low and influenced by various social and economic factors. Therefore, methods for improving life satisfaction level of low income and living alone old people are required for discerning our society's equality and healthiness.

신규 살균제 KNF 1002의 오이 및 고추 중 잔류특성

김태화,이재영,유용만,김장억 한국환경농학회 2003 한국환경농학회지 Vol.22 No.3

Methoxyacrylate계 신규 살균제 KNF 1002의 오이, 고추 및 고춧잎에 대한 잔류 양상을 조사하기 위하여 포장시험을 수행하였다. HPLC-DAD에 의한 KNF 1002의 잔류분석시 최소검출량은 오이 및 고추에서 2.0 ng 그리고 고춧잎에서는 2.5 ng이었으며, 검출한계는 오이 및 고추에서 0.02 mg/kg 그리고 고춧잎에서 0.05 mg/kg 이었다. 오이에서의 잔류량은 1회 및 2회 처리하여 1일에서 7일후에 채취된 시료에서 2.0 mg/kg에서 검출한계 미만으로 나타났다. 고추에서의 잔류량은 시설재배지의 경우 최고 0.79 mg/kg에서 0.31 mg/kg 수준으로 나타났다. 노지재배의 경우에는 0.47 mg/kg에서 0.11 mg/kg으로 나타나 시설재배의 47% 수준의 잔류량을 보였다. 고춧잎에서는 시설재배지의 경우 최고 25.20 mg/kg에서 7.38 mg/kg으로 나타났으며 노지재배의 경우에는 1.99 mg/kg에서 0.11 mg/kg 수준으로 나타나 시설재배에 비해서 6.2%정도의 잔류량을 보였다. This study was conducted to evaluate the terminal residue of a new fungicide, KNF 1002, in cucumber and pepper under greenhouse and field conditions. When a microemulsion formulation (20%) of KNF 1002 was applied once or twice during 1-7 days before harvest, its terminal residue in cucumber ranged <0.02-0.20 ㎎/㎏ under greenhouse condition. In pepper, its figure recorded 0.31-0.79 ㎎/㎏ and 0.11-0.28 ㎎/㎏ under greenhouse and field conditions, respectively. Much higher level of terminal residues was observed in leaves than those in fruits in pepper, showing 7.38-25.20 ㎎㎏ and 0.11-1.99 ㎎㎏ under greenhouse and field conditions, respectively. Cultivation condition affected evidently the residue level in pepper harvests. Residual pattern of KNF 1002 seemed to be comparable to strobilurin fungicides currently used.

석면폐증 1례

이영수,장태원,유호대,정만홍,이용환,서지영,허 방,이재성 大韓産業醫學會 1999 대한직업환경의학회지 Vol.11 No.1

Asbestosis is the disease of pulmonary fibrosis caused by the inhaled asbestos fibers, and could be diagnosed clinically, in the case of exposure history to asbestos is proved, by clinical symptoms of dyspnea or dry cough, physical examonation findings, and the radiographic features. But many othder inorganic dusts would show similar findings in the chest radiogram and sometimes the exposure history is obscure, so for the exact diagnosis of asbestosis lung biopsy is needed. In Korea, there have been some reports of survey in the workplace where asbestos is handled or of asbestos related diseases. This is a case report of asbestosis with accompanying pleural plaques, who had the occupational exposure to asbestos for 30 years and the consistent clinical, radiographic and pathological findings in the lung tissue obtained by the videoscope assisted thoracoscopic biopsy(VATS).