http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Allelic analysis of low molecular weight glutenin subunits using 2-DGE in Korean wheat cultivars
Lee, Jong-Yeol,Jang, You-Ran,Beom, Hye-Rang,Altenbach, Susan B.,Lim, Sun-Hyung,Lee, Choung Keun Japanese Society of Breeding 2017 Breeding science Vol.67 No.4
<P>Two-dimensional gel electrophoresis (2-DGE) was used as a complement to SDS-PAGE to determine the allelic compositions of LMW-GS in 32 Korean wheat cultivars. Protein patterns generated by 2-DGE from each cultivar were compared to patterns from standard wheat cultivars for each allele. At the <I>Glu-A3</I> locus, thirteen <I>c</I>, twelve <I>d</I>, three <I>e</I> (null), two g and two new alleles were identified. At the <I>Glu-B3</I> locus, one <I>b</I>, nineteen <I>d</I>, four <I>h</I>, one <I>i</I> and five <I>ad</I> alleles were identified. At the <I>Glu-D3</I> locus, twenty-three <I>a</I>, four <I>b</I>, four <I>c</I> and one <I>l</I> alleles were identified. When compared to results obtained previously using SDS-PAGE, there were discrepancies in the allelic designations of 10 of 32 cultivars (31%). While SDS-PAGE is a rapid and relatively simple method for assessing LMW-GS composition, the similar mobilities of the proteins makes it difficult to discriminate certain alleles. 2-DGE is a more complicated technique, but provides a more accurate picture of the complement of the LMW-GS in a given cultivar. In addition to providing essential information for wheat breeders, the 2-DGE reference maps generated in this study will make it possible to study the contributions of individual LMW-GS to flour quality.</P>
GSH-dependent peroxidase activity of the rice (Oryza sativa) glutaredoxin, a thioltransferase
Lee, Kyun-Oh,Lee, Jung-Ro,Yoo, Ji-Young,Jang, Ho-Hee,Moon, Jeong-Chan,Jung, Bae-Gyo,Chi, Yong-Hun,Park, Soo-Kwon,Lee, Seung-Sik,Lim, Chae-Oh,Yun, Dae-Jin,Cho, Moo-Je,Lee, Sang-Yeol Plant molecular biology and biotechnology research 2002 Plant molecular biology and biotechnology research Vol.2002 No.-
Glutaredoxin (Grx) is a 12-kDa thioltransoferase that reduces disulfide bonds of other proteins and maintains the redox potential of cells. In addition to its oxidoreductase activity, we report here that a rice Grx(OsGrx) can also function as a GSH-dependent peroxidase. Because of this antioxidant activity, OsGrx protects glutamine synthetase from oxidative damage. Individually replacing the conserved Cys residues in OsGrx with Ser shows that Cys^(23), but not Cys^(26), is essential for the thioltransferase and GSH-de-pendent peroxidase activities. Kinetic Characterization of OsGrx reveals that the maximal catalytic efficiency (V_(max)/K_(m))is obtained with cumene hydroperoxide rather than H_(2)O_(2) or t-butyl hydroperoxide.
Molecular and functional characterization of a PEX14 cDNA from rice
Lee, Jung-Ro,Lee, Kyun-Oh,Park, Jin-Ho,Yoo, Ji-Young,Kang, Jae-Sook,Jeon, Hye-Sook,Kim, Sun-Young,Lee, Young-Mi,Kim, Sun-Tae,Lim, Chae-Oh,Bahk, Jeong-Dong,Cho, Moo-Je,Lee, Sang-Yeol Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-
In contrast to the translocation mechanisms determined in yeasts and mammalian cells, there is little information on the functions of plant peroxisomal proteins or their genomic structures. To understand the role that PEX14 plays in diverse plant peroxisomal functions and how peroxisomal translocation is mediated in plant cells, we cloned a 1827 bp cDNA encoding the peroxisomal membrane protein OsPex14p from a rice leaf cDNA library. The 54kDa OsPex14p, which has a theoretical pI value of 6.06, contains a highly conserved N-terminal domain and a short putative transmembrane domain. The OsPEX14 gene in the rice genome exists as a single-copy gene, consists of eleven exons interrupted by ten introns, and spans about 5kb of chromosome 5. The 5′-flanking region contains putative cis-acting light-responsive elements, and the transcription initiation site maps 114bp upstream of the translation start codon. OsPEX14 mRNA is highly expresssed in leaf tissues and is induced by exposure to several stresses. Heterologous expression of OsPex14p suppresses the defect in targeting of peroxisomal matrix proteins in a pex14 null mutant of Saccharomyces cerevisiae.
Lim, Chae-Oh,Lee, Soo-In,Lee, Sang-Hyeung,Kim, Jong-Chel,Choi, Young-Ju,Bahk, Jeong-Dong,Lee, Sang-Yeol,Hwang, In-Hwan,Hong, Jong-Chan,Cho, Moo-Je Plant Molecular Biology & Biotechnology Research C 1994 Plant molecular biology and biotechnology research Vol.1994 No.
A cDNA clone encoding a cysteine proteinase inhibitor was obtained from ESTs of Chinese cabbage flower buds. The cDNA clone spans 784 base pairs, encoded 199-amino acid residues. BCPI contains the conserved Q-V-V-A-R sequence that exists in most members of the cystatin superfamily. Northern blot analysis showed that the amount of mRNA for BCPI was transcribe into a single mRNA species of about 800 nucleotides. BCPI was highly expressed in flower bud. Genomic southern blot analysis showed that BCPI is encoded by a small multigene family consist of at least two members. The protein expressed on E. coli inhibited strongly enzymatic activity of papain, a cysteine proteinase.
A Routine System for Generation of Fertile Transgenic Rice Plants Using Biolistic Method
Lee, Soo-In,Shon, Young-Geol,Kim, Cha-Young,Lim, Chae-Oh,Choi, Young-Ju,Kim, Ho-ll,Lee, Sang-Yeol,Lee, Sung-Ho Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-
A routine system based on particle bombardment of embryogenic callus for recovery of fertile transgenic rice (Oryza sativa L.) plants was developed. Embryogenic callus was established within 2-3 months from calli derived from mature seeds of Korean rice cultivar, Nagdongbyeo. The callus was bombarded with the plamid pRQ6 containing the β-glucuronidase gene (gusA) and hygromycin phosphotransferase gene (hph, conferring resistance to hygromycin B), both driven by CaMV 35S promoter. Placement of cells on an osmoticum-containing medium (0.2 M sorbitol and 0.2 M mannitol) 4 hrs prior to and 16 hrs after bombardment resulted in a statistically significant increase with 3.2-fold in transient expression frequency gusA. In five independent experiments, the average frequency of transformation showing GUS activities was 8.86%. A large number of morphologically normal, fertile transgenic rice plants were obtained. Integration of foreign gene into the genome of R_(0) transgenic plants was confirmed by Southern blot analysis. GUS and HPT were detected in R_(1) progeny and Mendelian segregation of these genes was observed in R_(1) progeny.
CHARACTERIZATION OF A NOVEL DIVERGENT CALMODULIN ISOFORM FROM SOYBEAN
Lee, Sang-Hyoung,Kim, Jong-Cheol,Lee, Mal-Soon,Cheong, Yong-Hwa,Yoon, Hae-Won,Lim, Chae-Oh,Hong, Jong-Chan,Bahk, Jeong-Dong,Lee, Sang-Yeol,Hwang, In-Hwan,Cho, Moo-Je Plant Molecular Biology & Biotechnology Research C 1993 Plant molecular biology and biotechnology research Vol.1993 No.
Lee, Won-Young,Hong, Joon-Ki,Kim, Cha-Young,Chun, Hyun-Jin,Park, Hyeong-Cheol,Kim, Jong-Cheol,Yun, Dae-Jin,Chung, Woo-Sik,Lee, Sung-Ho,Lee, Sang-Yeol,Cho, Moo-Je,Lim, Chae-Oh Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-
A cDNA encoding RARF1 (rice ADP-ribosylation factor 1) was isolated from fungal elicitor-treated rice suspension culture cells by mRNA differential display. RARF1 transcripts accumulated in response to hydrogen peroxide (H_(2)O_(2)) and salicylic acid (SA) and rapidly in cells inoculated with an avirulent pathogen. Constitutively over-expressed RARF1 under the control of the cauliflower mosaic virus 35S promoter (CaMV 35S) triggered spontaneous induction of lesion mimics, induced an array of pathogenosis-related (PR) genes, reduced susceptibility to a fungal pathogen, and caused accumulation of SA. From these data, we deduced that RARF1 might be a component of various plant defence signaling pathways involved in inducing the expression of a subset of PR genes.
A Routine System for Generation of Fertile Transgenic Rice Plants Using Biolistic Method
Lee Soo-In,Kim Cha-Young,Lim Chae-Oh,Choi Young-Ju,Kim Ho-Il,Lee Sang-Yeol,Lee Sung-Ho The Korean Society of Plant Biotechnology 2003 Plant molecular biology and biotechnology research Vol.5 No.3
A routine system based on particle bombardment of embryogenic callus for recovery of fertile transgenic rice (Oryza sativa L.) plants was developed. Embryogenic callus was established within 2-3 months from calli derived from mature seeds of Korean rice cultivar, Nagdongbyeo. The callus was bombarded with the plasmid pRQ6 containing the $\beta$-glucuronidase gene (gusA) and hygromycin phosphotransferase gene (hph, conferring resistance to hygromycin B), both driven by CaMV 35S promoter. Placement of cells on an osmoticum-containing medium (0.2 M sorbitol and 0.2 M mannitol) 4 hrs prior to and 16 hrs after bombardment resulted in a statistically significant increase with 3.2-fold in transient expression frequency gusA. In five independent experiments, the average frequency of transformation showing GUS activities was $8.86\%$. A large number of morphologically normal, fertile transgenic rice plants were obtained. Integration of foreign gene into the genome of $R_0$ transgenic plants was confirmed by Southern blot analysis. GUS and HPT were detected in $R_1$ progeny and Mendelian segregation of these genes was observed in $R_1$ progeny.
Lee, Moo-Yeol,Lee, Young-Ho,Lim, Kyung-Min,Chung, Seung-Min,Bae, Ok-Nam,Kim, Heon,Lee, Choong-Ryeol,Park, Jung-Duck,Chung, Jin-Ho National Institute of Environmental Health Science 2005 Environmental health perspectives Vol.113 No.10
<P>Chronic exposure to arsenic is well known as the cause of cardiovascular diseases such as hypertension. To investigate the effect of arsenic on blood vessels, we examined whether arsenic affected the contraction of aortic rings in an isolated organ bath system. Treatment with arsenite, a trivalent inorganic species, increased vasoconstriction induced by phenylephrine or serotonin in a concentration-dependent manner. Among the arsenic species tested—arsenite, pentavalent inorganic species (arsenate), monomethylarsonic acid (MMA<SUP>V</SUP>), and dimethylarsinic acid (DMA<SUP>V</SUP>)—arsenite was the most potent. Similar effects were also observed in aortic rings without endothelium, suggesting that vascular smooth muscle plays a key role in enhancing vasoconstriction induced by arsenite. This hypercontraction by arsenite was well correlated with the extent of myosin light chain (MLC) phosphorylation stimulated by phenylephrine. Direct Ca<SUP>2+</SUP> measurement using fura-2 dye in aortic strips revealed that arsenite enhanced vasoconstriction induced by high K<SUP>+</SUP> without concomitant increase in intracellular Ca<SUP>2+</SUP> elevation, suggesting that, rather than direct Ca<SUP>2+</SUP> elevation, Ca<SUP>2+</SUP> sensitization may be a major contributor to the enhanced vasoconstriction by arsenite. Consistent with these <I>in vitro</I> results, 2-hr pretreatment of 1.0 mg/kg intravenous arsenite augmented phenylephrine-induced blood pressure increase in conscious rats. All these results suggest that arsenite increases agonist-induced vasoconstriction mediated by MLC phosphorylation in smooth muscles and that calcium sensitization is one of the key mechanisms for the hypercontraction induced by arsenite in blood vessels.</P>