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McCoy 세포에서 Chlamydia trachomatis의 병원성 인자에 관한 연구
강정숙,최지영,차영주,김영주,김덕례,여명구,박열 조선대학교 생명과학연구소 1998 생명과학 연구 Vol.6 No.-
본 연구는 병원성 세균인 Chlamydia trachomatis(C. trachomatis)가 숙주세포에침습시 C. trachomatis의 병원성 인자와 C. trachomatis와 상호작용하는 숙주 세포막의 인자를 규명함으로써 숙주세포에 대한 감염율을 향상시켜 보다 향상된 진단 방법을 개발하기 위하여 수행하였다. C. trachomatis로 감염된 세포에서 기본소체의 막 단백질을 분리한 결과 각 혈청형의 39 kDa과 42 kDa에서 major outer membranes(MOMPs)이 관찰되었으며, 혈청형 E와 G의 MOMPs는 42.5 kDa으로 동일하였지만 혈청형 F, H, I, 그리고 K의 MOMPs는 각각 다르게 관찰되었다. C. trachomatis가 숙주세포로 침습시 관련된 병원성 인자에 대하여 조사하고자 단층세포로 배양된 배양용기에 C. trachomatis를 24, 48 그리고 73시간대별로 접종시켜 관찰한 결과 72시간의 MOMPs의 양이 가장 많이 증가하였으며 이 결과로 MOMPS이 C. trachomatis 병원성에 중요한 작용을 함을 확인할 수 있었다. C. trachomatis가 침습시 숙주세포의 막 단백질 변화양상을 관찰하기 위해 정상세포와 C. trachomatis로 1, 2, 3 그리고 4시간대별로 접종 시간을 달리한 숙주세포의 막 단백질을 분리한 결과 42 kDa의 단백질 양이 Chlamydia에 감염된 세포 내에서 증가됨을 관찰할 수 있었다. 이로 42 kDa의 막 단백질이 침습시 숙주세포 표면에 대한 수용체로서 작용함을 알 수 있었다. The present study was performed to analyze the pathogenic factors involved in the invasion process of Chlamydia trachomatis(C. trachomatis) into McCoy cells. In this study, elementary bodies(EBs) of C. trachomatis serotypes(E, F, G, H, I, K and LGV) could be isolated by the method of Percoll density gradient centrifugation and the pathogens of serotypes were also compared from whole-cell lysates by using sodium dodecyl sulfate-polyacrylamide gel elelectrophoresis. All chlamydial serotypes showed a single predominant protein ranging from 39 to 42 kDa. The major outer membrane proteins(MOMPs) of the E and G serotypes had an identical molecular weight of 42.5 kDa. In contrast, the MOMPs of the F, H, I and K serotypes were showed different molecular weights of MOMPs. By immunoblotting with anti-MOMP antibody it was revealed that the quantify of MOMPs was time-dependently increased when McCoy cells were infected with Chlamydia for 24, 48 and 72 hr. These results indicated that MOMPs are closely related to chlamydial infection into the host cell.
Yeo Myeong Lee,조한진,Shree Priya Ponnuraj,김종대,김정상,김상건,윤정한 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.4
Phenethyl isothiocyanate (PITC) is the hydrolysis product of the glucosinolate gluconasturtiin in cruciferous vegetables. This study was conducted to determine whether PITC inhibits 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation in the mouse ear. Topical application of 5 nmol of TPA to mouse ears markedly increased the ear weight, expression of the inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 protein, and phosphorylation of the inhibitor of κB (IκB) α, AKT, and extracellular signal-regulated protein kinase (ERK) 1/2 and reduced IκBα protein levels. Pretreatment with PITC (150–450 nmol) significantly suppressed these TPA-induced inflammatory responses. We also determined whether low concentrations of PITC (0.5–5 μmol/L) inhibited lipopolysaccharide (LPS)-stimulated inflammatory responses in Raw264.7 cells. PITC dose-dependently reduced the LPS-induced secretion of nitric oxide, prostaglandin E2, interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α, as well as COX-2 and iNOS protein expression. PITC also attenuated LPS-induced increases in iNOS, COX-2, IL- 6, IL-1β, and TNF-α mRNA levels, as well as the promoter-dependent transcriptional activation of the genes for iNOS and COX-2. PITC inhibited LPS-induced IκBα phosphorylation and degradation and subsequently reduced LPS-induced p65 nuclear translocation and the transcriptional activity of nuclear factor-κB (NF-κB), which was accompanied by a reduction in ERK1/2 and AKT phosphorylation. The results of this study demonstrated that PITC effectively inhibits inflammatory responses in vivo and in vitro, which may be mediated via the inhibition of AKT and ERK1/2 activation, leading to subsequent inhibition of the transcriptional activity of NF-κB.
Diagnosis of Chlamydial infection by using monoclonal antibody
Yeo, Myeong-Gu,Kim, Ho-Sang,Kim, Young-Ju,Kim, Sung-Jun,Kim, Jong-Se,Choi, Young-Bok,Park, Yeal 조선대학교 생명과학연구소 1997 생명과학 연구 Vol.5 No.-
Hybrid cell line that produced MAbs against Chlamydia trachomatis surface antigens were prepared by the fusion of mouse myeloma cells with BALB/c mouse. Those Mabs were used to examine the antigenic specificity and biological function of several Chlamydia serotypes. Using those MABs, chlamydial inclusions were detected by using chemiluminence and it turns out that the chemiluminents method was very useful to detect the chlamydial inclusions or infection. It was confirmed that this method is easy to detect and to reduce the time consuming compared with examination of chlamydial inclusions order fluorescence microscopy.
Yeo-Myeong Kim,Han-Byeol Kang,Gi-Heon Kim,Chi-Sun Hwang,Sung-Min Yoon IEEE 2017 IEEE electron device letters Vol.38 No.10
<P>Technical strategies for improving the device characteristics of the In-Ga-Zn-O (IGZO) vertical channel thin-film transistors (VTFTs) were presented and investigated. The vertical sidewall was constructed by dry-etch process and subsequently covered with IGZO, Al2O3, and AZO as active, gate insulator, and gate electrode layers by means of conformal atomic-layer-deposition. An abrupt profile and flat back-channel were achieved by employing the spin-coated polyimide (PI) spacer. The Off-current was additionally alleviated simply by cutting the area of an active layer. The fabricated IGZO VTFT using PI spacer with an 'active-cut' structure exhibited an On/off ratio of 10(3), a linear mobility of 7.1 cm(2)/Vs, and a subthreshold swing of 1.2 V/decade.</P>
( Yeo Myeong Yun ),( Dong Hoon Kim ),( Hang Sik Shin ) 한국폐기물자원순환학회(구 한국폐기물학회) 2013 한국폐기물자원순환학회 추계학술발표논문집 Vol.2013 No.-
Waste activated sludge (WAS) and food waste (FW) are available year round at low cost and have the potential to promote synergism in anaerobic digestion (AD). The goal of this study was to clarify the synergism in co-digestion of WAS and FW. A slight amount of FW at various ratios was added to WAS as an auxiliary substrate, and anaerobic batch tests were performed under mesophilic conditions. By adding FW, total CH4 produced was increased, where most of them were come from WAS, clearly suggesting synergism. Also, lag period was shortened and CH4 production rate was increased by FW addition. It was hypothesized that enhanced performance was owing to the facilitated hydrolysis of WAS by FW addition, which was revealed by the increased activities of hydrolytic -amylase and protease.