Correlation between DNA methylation and Thymic Stromal Lymphopoietin expression in asthmatic airway epithelial cells

Yan‑Li Li,Xi‑Qian Xing,Yi Xiao,Yan‑Hong Liu,Yu‑Shan Zhou,Min Zhuang,Chao‑Qian Li 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: The overexpression of TSLP and DNA methylation in asthma were both risk factors the relationship was not clear. Objective: This study aimed to investigate the relationship between methylation status of TSLP promoter and mRNA/protein expression in asthmatic airway epithelial cells. Methods: Human bronchial epithelial cells were cultured in vitro and divided into: Control group, treated with PBS, model group, sensitized with LPS (10 μg/mL) for 12 h (37 °C, 5% CO2). Other groups were cultured with the pCMV3 plasmid (M + NC/pCMV), pGPH1 plasmid (M + NC/pGPH), DNMT1/pCMV3 plasmid (M + DNMT1/pCMV), and DNMT1/pGPH1 plasmid (M + DNMT1/pGPH) for 48 h. The expression of DNA methyltransferase 1 and TSLP were measured using real-time PCR and western blotting. Results: Compared with the control group, TSLP mRNA (1.00 ± 0.00 vs. 2.82 ± 0.81 vs. 1, P < 0.001) and protein (1.07 ± 0.04 vs. 1.46 ± 0.11, P < 0.01) were significantly greater, and the methylation of promoter was lower (92.75 ± 1.26 vs. 58.57 ± 3.34, P < 0.05) in the model group. Compared with the model group, TSLP mRNA (2.82 ± 0.81 vs. 1.17 ± 0.10, P < 0.001) decreased, but TSLP promoter methylation increased (58.57 ± 3.34 vs. 92.58 ± 7.30, P < 0.05) in M + DNMT1/pCMV. TSLP mRNA and protein were higher (2.82 ± 0.81 vs. 5.32 ± 0.21, P < 0.001; 1.46 ± 0.11 vs. 1.94 ± 0.11, respectively, P < 0.01), TSLP promoter methylation was lower (58.57 ± 3.34 vs. 33.57 ± 4.29, P < 0.05) in M + DNMT1/pGPH. Conclusions: Overexpression of TSLP in asthmatic airway epithelial cells may be regulated by DNA demethylation.

Les idees et la traduction

YAN,Suwei 한국통역번역학회 2005 FORUM Vol.3 No.1

Analysis of genetic diversity among indigenous landraces from sesame (Sesamum indicum L.) core collection in China as revealed by SRAP and SSR markers

Yan‐xin Zhang,Wei Hua,Lin‐han Wang,Zhuo Che,Xiu‐rong Zhang 한국유전학회 2010 Genes & Genomics Vol.32 No.3

The molecular genetic diversity of 404 indigenous landraces from sesame core collection in China were evaluated by 11SRAP and 3 SSR markers, 175 fragments were generated, of which 126 were polymorphic with an average polymorphism rate of 72%. Jaccard’s genetic similarity coefficients (GS=0.7130), Nei's gene diversity (h=0.2418) and Shannon's Information index (I=0.3847) were calculated, a dendrogram of the 404 landraces was made, landraces from various zones were distributed throughout the dendrogram, accessions from different agro‐ecological zones were indistinguishable by cluster analysis, geographical separation did not generally result in greater genetic distance, a similar pattern was obtained using principal coordinates (PCO) analysis. As to seven agro‐ecological zones, the maximum Nei’s gene diversity (h = 0.2613)and Shannon index (I = 0.3980) values in zone VII indicated that they were genetically more diverse than those in other zones, while the least genetically diverse region was zone III (h = 0.1772, I = 0.2858). Nei's genetic identity and genetic distance among landraces from seven agro‐ecological zones were also analyzed, the genetic relationship of seven zones was inferred using the UPGMA method. This study demonstrated that SRAP and SSR markers were appropriate for evaluation of sesame genetic diversities. There existed extensive genetic diverse among indigenous landraces and the abundance of genetic diversity of landraces in different agro‐ecological zones was various. Understanding of these characteristics of indigenous landraces in China can provide theoretical foundation for further collection, effective protection and reasonable utilization of these sesame landraces in breeding.

Intragranular and Intergranular Crack Propagation in Nanocrystalline Ni Under Single-Cycle Mode I Loading

Yan Feng,Jie Li,Xinhua Yang 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.7

As the first step to study the fatigue crack propagation, the molecular simulations were performed to investigate theopening, propagation and closure behaviors of intragranular and intergranular cracks in nanocrystalline Ni under single-cyclemode I loading. The atomic crack angle was proposed to characterize the crack-tip blunting and sharpening quantitatively. There are obvious different mechanisms between intragranular and intergranular crack propagation. Intragranular crack propagationcould be induced by stacking faults and can change its direction very easily, but the intergranular crack perpendicularto the loading direction would propagate along the grain boundary and encounter strong resistance at the triple junction. New cracks could form in the grain boundaries ahead of the original crack. Different from the traditional understanding forintragranular and intergranular cracks, however, the dislocation density increases even in the unloading process and the cracktip is possibly sharpened temporarily during loading.

An On-Demand Routing Algorithm for Mobile Wireless Networks

Yan, Huai-Zhi,Ajith P.K,Park, Dong-Won,Joo, Gi-Ho 배재대학교 공학연구소 2005 공학논문집 Vol.7 No.1

An ad hoc network is a collection of wireless mobile nodes forming a temporary network without the centralized administration. Due to the limited transmission range of wireless work interface, multiple networks “hops” maybe needed for one node to exchange data with adjacent node. In recent years, a variety of new routing protocol about ad hoc network was developed. This paper presents a new routing protocol based on the Dynamic Source Routing which is not suitable for the high mobility ad hoc network. The Enhanced DSR adapts quickly to routing changes when node movement is frequent. When a trunk route is broken, this protocol will utilize the alternative route saved in the route to discovery quickly the new route. It improves the performance of the existing DSR algorithm, so that the negative impacts from weakness of DSR are reduced.

Situations and Roles: “Danmu” (Comment-Barrage) Culture in Media Situation

An Yan,Jian Zhi Cai 중앙대학교 영상콘텐츠융합연구소 2018 TechArt :Journal of Arts and Imaging Science Vol.5 No.1

거주 경험과 상호작용 정도에 따른 한국어 사회문화적 함축 이해 -중국인 고급 한국어 학습자를 대상으로-

이암 ( Li¸ Yan ),이지현 ( Lee¸ Jihyun ) 국제한국어교육학회 2020 한국어 교육 Vol.31 No.3

This study aims to analyze how advanced Chinese Korean learners understand social and cultural implicatures based on their residential experience in Korea and the intensity of interaction. Research questions for this paper are as follows: (1) Which of the social and cultural domains is difficult to understand? (2) Does the residential experience in Korea and intensity of interaction impact their understanding of implicatures? If so, which is the most influential factor? According to the results of the experiment, Chinese learners did not have high scores in all implications; in particular, they had the lowest understanding of implicatures of the political realm. In addition, both the intensity of interaction and residential experience influenced the understanding of implications, and therefore, the latter has become the biggest factor in understanding sociocultural implicatures. (Dalian University of Foreign Languages Ewha Womans University)

중세 국어 비자동적 교체 체언 어간의 단일화

이하얀(Lee, Ha-yan) 한국어문학회 2017 語文學 Vol.0 No.135

This study aims to reveal a principle in the changing pattern of the uninflected word, which phenomenon is prevalent in the Middle Korean. To achieve this goal, this article focuses on the nonautomatic change found in the stem of the uninflected word such as namu (나무) . In previous studies, some scholars attempted to explain the reason, they failed to give an explanation of the principle behind the phenomenon that raises a question regarding the function of verb and noun. Also, the theoretical explanation of the usage of uninflected word, which has been obscure, and it does not allow readers to clearly understand it. This article therefore focuses on the independent nature and function of the uninflected word in Middle Korean. In my analysis of the uninflected word, I suggest separating the part of the uninflected word into two variants which cause nonautomatic changes of the uninflected word. In this study, both the historical data and dialects of Modern Korean language are employed to prove this point. In addition, I propose that ‘-(eu(으))X-’ ending may have been a key to understand the phenomenon.

“选贤任能”与国家治理现代化: 历史梳理与制度分析

应验 ( Ying¸ Yan ) 성균관대학교 성균중국연구소 2021 중국사회과학논총 Vol.3 No.2

“选贤任能”是党的十九届四中全会提出的国家制度和治理的显著优势之一。“选贤任能”制度包含了“任人唯贤”的干部路线和“德才兼备”的干部标准, 并具有代表性强、选拔全面、有利于稳定等比较优势。“选贤任能”这一理念自原始洪荒时期起萌芽, 后经历禅让制、世袭制、察举制、科举制、委任制、选任制、考任制等模式流变, “贤能”的标准与“选任”的程序得到了极大的丰富。从制度理论上分析, “选贤任能”制度又体现出了制度变迁与制度依赖的特点, 在一定时期促进了国家的稳定和治理的有序, 但也阻碍了国家的转型发展。在新时代, 要继续推进“选贤任能”制度的革新, 使其更好适应国家治理现代化的需求。 Selection plus election (选贤任能制度), a modernized version of Confucian meritocracy, is written as a key strategy for the stability of the China Communist Party (CPC) leadership system. For short, the selection plus election system is a personnel system with Chinese characteristics and it has strong vitality and strength as proven by practice. The selection plus election system is a scientific system formed from the primitive flood and famine period (洪荒时期), andthen experienced the changes of models such as abdication system (禅让制), hereditary system (世袭制), examination system (察举制), imperial examination system (科举制), appointment system (委任制), and selection system(选任制). Reforms and learning should be continued to promote the innovation of the selection plus election system to make it better adapt to the needs of the modernization of national governance in China. The selection plus election system is not elite politics, nor democracy merely based on popular election, but a hybrid system based more on meritocratic leadership. More understanding and tolerance should be given to this experiment and further research should be conducted to analyse this system as well.

Nodule Rich Protein 2 modulates nodule number in Medicago truncatula

Junhui Yan,Xinwei Yang,Yawen Wang,Liangliang Yu,Li Luo 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.1

Symbiotic nitrogen fxation is beneft to sustainable agriculture and global nitrogen cycle. Many small peptides were identifed as regulators involving in the interaction between rhizobia and legume. Here we reported Nodule Rich Protein 2 (MtNRP2) encoding a small peptide in Medicago truncatula, belonged to a group of nodule rich protein restricted in legume species. MtNRP2 expressed highly in root nodule and its promoter was active during the initiation and development of root nodule and lateral root. To investigate the function of MtNRP2 in nodulation, we generated MtNRP2-overexpression and MtNRP2- knockdown transgenic Medicago. MtNRP2-overexpression transgenic lines performed normal nodulation phenotype compared with vector control. However, in the MtNRP2-RNAi transgenic plants, the decrease of MtNRP2 expression lead to the increase of infection threads number (7 day post inoculation) and nodules number (3 week post inoculation); meanwhile, the expression of MtRGF3 and MtPUB1 was inhibited. These results suggested that MtNRP2 negatively regulated nodulation in Medicago truncatula.