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This paper combed the Mo Yan’s plays from 1978 to 2017. It simply analyzed his film scripts and TV scripts. The films and TV series promoted the popularization of Mo Yan’s works. And in turn, because the writers participated in the creation of the films and TV series, so it improved the artistic taste of the film and TV series. They showed that Literature and the films and television series form a positive interaction. This paper analyzed the drama scripts which were independently created by Mo Yan: “Farewell My Concubine” “The Boiler Man’s Wife” “Our Jing Ke” and “Beautiful Clothes”. By Mo Yan’s personal understanding and the writing method of realism and romanticism, the drama scripts shaped the typical characters. It revealed the inner world of the characters. The works combined history, folk and real society organically. He reinterpreted the history in a literary way.
Background: The overexpression of TSLP and DNA methylation in asthma were both risk factors the relationship was not clear. Objective: This study aimed to investigate the relationship between methylation status of TSLP promoter and mRNA/protein expression in asthmatic airway epithelial cells. Methods: Human bronchial epithelial cells were cultured in vitro and divided into: Control group, treated with PBS, model group, sensitized with LPS (10 μg/mL) for 12 h (37 °C, 5% CO2). Other groups were cultured with the pCMV3 plasmid (M + NC/pCMV), pGPH1 plasmid (M + NC/pGPH), DNMT1/pCMV3 plasmid (M + DNMT1/pCMV), and DNMT1/pGPH1 plasmid (M + DNMT1/pGPH) for 48 h. The expression of DNA methyltransferase 1 and TSLP were measured using real-time PCR and western blotting. Results: Compared with the control group, TSLP mRNA (1.00 ± 0.00 vs. 2.82 ± 0.81 vs. 1, P < 0.001) and protein (1.07 ± 0.04 vs. 1.46 ± 0.11, P < 0.01) were significantly greater, and the methylation of promoter was lower (92.75 ± 1.26 vs. 58.57 ± 3.34, P < 0.05) in the model group. Compared with the model group, TSLP mRNA (2.82 ± 0.81 vs. 1.17 ± 0.10, P < 0.001) decreased, but TSLP promoter methylation increased (58.57 ± 3.34 vs. 92.58 ± 7.30, P < 0.05) in M + DNMT1/pCMV. TSLP mRNA and protein were higher (2.82 ± 0.81 vs. 5.32 ± 0.21, P < 0.001; 1.46 ± 0.11 vs. 1.94 ± 0.11, respectively, P < 0.01), TSLP promoter methylation was lower (58.57 ± 3.34 vs. 33.57 ± 4.29, P < 0.05) in M + DNMT1/pGPH. Conclusions: Overexpression of TSLP in asthmatic airway epithelial cells may be regulated by DNA demethylation.
Mobile Web has become the most influential technology in this era, owing to which, IoE (Internet of Everything) is virtually materialized, which enables a single medium to carry various information and construct situations for experiencing, linking and interacting for social networking, media playing, gaming, shopping and educational apps. It also connects the Small Worlds, in Duncan J. Watts’ words. In this new interactive system, informational flows are bilateral, which means individuals not only just take in information but also give out their views and emotional feelings on the Net. They express themselves and interact with each other, further defining their new social roles online. This paper, a research on the basis of Meyrowitz’s “situational approach”, attempts to inquire the connotation of the word “situation” under the context of IoE era, and then study the impacts of “danmu” on Internet users’ behaviors and social roles.
An ad hoc network is a collection of wireless mobile nodes forming a temporary network without the centralized administration. Due to the limited transmission range of wireless work interface, multiple networks “hops” maybe needed for one node to exchange data with adjacent node. In recent years, a variety of new routing protocol about ad hoc network was developed. This paper presents a new routing protocol based on the Dynamic Source Routing which is not suitable for the high mobility ad hoc network. The Enhanced DSR adapts quickly to routing changes when node movement is frequent. When a trunk route is broken, this protocol will utilize the alternative route saved in the route to discovery quickly the new route. It improves the performance of the existing DSR algorithm, so that the negative impacts from weakness of DSR are reduced.
The molecular genetic diversity of 404 indigenous landraces from sesame core collection in China were evaluated by 11SRAP and 3 SSR markers, 175 fragments were generated, of which 126 were polymorphic with an average polymorphism rate of 72%. Jaccard’s genetic similarity coefficients (GS=0.7130), Nei's gene diversity (h=0.2418) and Shannon's Information index (I=0.3847) were calculated, a dendrogram of the 404 landraces was made, landraces from various zones were distributed throughout the dendrogram, accessions from different agro‐ecological zones were indistinguishable by cluster analysis, geographical separation did not generally result in greater genetic distance, a similar pattern was obtained using principal coordinates (PCO) analysis. As to seven agro‐ecological zones, the maximum Nei’s gene diversity (h = 0.2613)and Shannon index (I = 0.3980) values in zone VII indicated that they were genetically more diverse than those in other zones, while the least genetically diverse region was zone III (h = 0.1772, I = 0.2858). Nei's genetic identity and genetic distance among landraces from seven agro‐ecological zones were also analyzed, the genetic relationship of seven zones was inferred using the UPGMA method. This study demonstrated that SRAP and SSR markers were appropriate for evaluation of sesame genetic diversities. There existed extensive genetic diverse among indigenous landraces and the abundance of genetic diversity of landraces in different agro‐ecological zones was various. Understanding of these characteristics of indigenous landraces in China can provide theoretical foundation for further collection, effective protection and reasonable utilization of these sesame landraces in breeding.
Symbiotic nitrogen fxation is beneft to sustainable agriculture and global nitrogen cycle. Many small peptides were identifed as regulators involving in the interaction between rhizobia and legume. Here we reported Nodule Rich Protein 2 (MtNRP2) encoding a small peptide in Medicago truncatula, belonged to a group of nodule rich protein restricted in legume species. MtNRP2 expressed highly in root nodule and its promoter was active during the initiation and development of root nodule and lateral root. To investigate the function of MtNRP2 in nodulation, we generated MtNRP2-overexpression and MtNRP2- knockdown transgenic Medicago. MtNRP2-overexpression transgenic lines performed normal nodulation phenotype compared with vector control. However, in the MtNRP2-RNAi transgenic plants, the decrease of MtNRP2 expression lead to the increase of infection threads number (7 day post inoculation) and nodules number (3 week post inoculation); meanwhile, the expression of MtRGF3 and MtPUB1 was inhibited. These results suggested that MtNRP2 negatively regulated nodulation in Medicago truncatula.
“选贤任能”是党的十九届四中全会提出的国家制度和治理的显著优势之一。“选贤任能”制度包含了“任人唯贤”的干部路线和“德才兼备”的干部标准, 并具有代表性强、选拔全面、有利于稳定等比较优势。“选贤任能”这一理念自原始洪荒时期起萌芽, 后经历禅让制、世袭制、察举制、科举制、委任制、选任制、考任制等模式流变, “贤能”的标准与“选任”的程序得到了极大的丰富。从制度理论上分析, “选贤任能”制度又体现出了制度变迁与制度依赖的特点, 在一定时期促进了国家的稳定和治理的有序, 但也阻碍了国家的转型发展。在新时代, 要继续推进“选贤任能”制度的革新, 使其更好适应国家治理现代化的需求。 Selection plus election (选贤任能制度), a modernized version of Confucian meritocracy, is written as a key strategy for the stability of the China Communist Party (CPC) leadership system. For short, the selection plus election system is a personnel system with Chinese characteristics and it has strong vitality and strength as proven by practice. The selection plus election system is a scientific system formed from the primitive flood and famine period (洪荒时期), andthen experienced the changes of models such as abdication system (禅让制), hereditary system (世袭制), examination system (察举制), imperial examination system (科举制), appointment system (委任制), and selection system(选任制). Reforms and learning should be continued to promote the innovation of the selection plus election system to make it better adapt to the needs of the modernization of national governance in China. The selection plus election system is not elite politics, nor democracy merely based on popular election, but a hybrid system based more on meritocratic leadership. More understanding and tolerance should be given to this experiment and further research should be conducted to analyse this system as well.
This study aims to reveal a principle in the changing pattern of the uninflected word, which phenomenon is prevalent in the Middle Korean. To achieve this goal, this article focuses on the nonautomatic change found in the stem of the uninflected word such as namu (나무) . In previous studies, some scholars attempted to explain the reason, they failed to give an explanation of the principle behind the phenomenon that raises a question regarding the function of verb and noun. Also, the theoretical explanation of the usage of uninflected word, which has been obscure, and it does not allow readers to clearly understand it. This article therefore focuses on the independent nature and function of the uninflected word in Middle Korean. In my analysis of the uninflected word, I suggest separating the part of the uninflected word into two variants which cause nonautomatic changes of the uninflected word. In this study, both the historical data and dialects of Modern Korean language are employed to prove this point. In addition, I propose that ‘-(eu(으))X-’ ending may have been a key to understand the phenomenon.
在國際文化交流日益頻繁的新世紀, 中國散已經開了對外開放的大門. 在文學藝術領域, 各式各樣的著名文學作品源源不斷地被譯介到國家外, 使世人遷漸認識和了解了有着懲久歷史的古老文明的國度他的昨天, 前天和今天的文化.然而, 作爲話劇,這一西洋泊來的文化藝衛品種, 在中國的歷史狼不太長, 特別是京味話劇的誕生和發展, 還未更多的被世人關注, 和了解. 筆者旨在介紹京味話劇初次走出國門從來曾創造過的輝煌, 竝分析. 探討京味話劇在新的歷史時期發展的前景和走向, 鐵世界深入了解中國, 認識北京, 提供一介審美的感性的舞臺,進一步促進中國和世界的文化交流活動.