http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Yarrowia lipolytica의 Multicopy Integration Vector 개발
김정윤,우문희,Dewey D.Y. Ryu 한국산업미생물학회 1995 한국미생물·생명공학회지 Vol.23 No.5
Multicopy integration vector는 복제수가 많고 non-selective한 환경조건 하에서도 매우 안정되게 유지가 되기 때문에 heterologous 유전자를 발현시키는데 매우 유용한 벡터 시스템이다. Yarrowia lipolytica의 multicopy integration vector를 개발하기 위하여 Y. lipolytica로부터 P-type rDNA를 클로닝하였다. 이 클론된 rDNA의 HindIII-BglII 절편과 promoter 지역을 포함하고 있지 않은 URA3 유전자를 pGEM1 plasmid에 삽입하여 제조한 벡터를 pMIYL-1과 pMIYL-2로 명명하였다. RDNA 절편은 벡터와 chromosomal DNA 사이에 homologous recombination을 유도하기 위한 것이며, promoterless URA3는 불완전한 표지 유전자로서 multicopy integration을 유발시키기 위한 것이다. PMIYL-1은 rDNA의 HindIII-BglII 절편내에 유일한 제한효소 자리로서 KpnI을 가지고 있고, pMIYL-2는 KpnI과 EcoRI을 가지고 있다. 이 벡터들을 Y. lipolytica에 도입한 후에 형질 전환체를 선별하여 copy 수와 안정성을 검사한 결과, 벡터의 copy 수는 5개 이하로 존재하고 non-selective 배지에서도 매우 안정하게 유지가 됨을 알 수 있었다. Multicopy integration vector is a very useful vector system in that they can be integrated into chromosomal DNA in several copies and stably maintained under non-selective conditions. To develop a multicopy integration vector system in the yeast Yarrowia lipolytica, P-type ribosomal DNA was cloned from Y. lipolytica. A HindIII-BglII fragment of the cloned rDNA and a promoterless URA3 gene were inserted into pGEM1, generating multicopy integration vectors, pMIYL-1 and pMIYL-2. The rDNA fragment is for targeted homologous recombination between the vector and the chromosomal DNA of Y. lipolytica, and the promoterless URA3 gene is a defective selection marker for inducing multicopy integration. pMIYL-1 and pMIYL-2 have an unique restriction enzyme site, KpnI, and two unique restriction enzyme sites, KpnI and EcoRI, repectively, which can be used for targeting of the vectors into the rDNA of Y. lipolytica chromosomal DNA. After transformation of the vectors into Y. lipolytica, copy number and stability were analyzed by Southern hybridization. The vectors were found to be present in less than 5 copies per cell and were stably maintained during growth in non-selective media.
공간벡터변조에 의한 반송용 편측식 선형유도전동기의 동특성에 관한 연구
우정인,조윤현,조용길,신동률,정영일 동아대학교 공과대학부설 생산기술연구소 1997 生産技術硏究所硏究論文集 Vol.2 No.2
A Single-sided Linear Induction Motor(SLIM) has been developed for use in industrial machines, transportation, application to automations, because it has merits of direct driving and simple structure. The SLIM for conveyer system requires a soft start and stop, and no shock drive. The air gap of SLIM is large and the fluctuation of its distance while being operated has significant effect on the thrust of SLIM and cause the thrust ripple. Mecause of the effect of this thrust ripple, it is difficult to control the speed of SLIM precisely. In order to improve the problems maintenance above, in this paper, the Space Vector Modulation (SVM) with PI controller is proposed, which offers the dynamic characteristics of SLIM without the thrust ripple.
간호대생들의 임상실습 스트레스, 학업적 자기효능감 및 진로정체감에 대한 연구
최유빈,최민지,이은형,이연우,우소연,하희주,허예지,박수현,허미래,이가현 이화여자대학교 간호과학대학 2015 이화간호학회지 Vol.- No.49
Purpose: The purpose of this study was to investigate clinical practice stress, academic self-efficacy, and career identity among undergraduate students in nursing practicum. Method: This study was a descriptive correlational design using the survey method. The study included 267 undergraduate students attending to 5 college in Seoul. The data was gathered from August 2014 to October 2014 and the data were analyzed using IBM SPSS statistics version 22.0 Program with descriptive statistics, t-test, analysis of variance with Scheffe test, and Pearson’s correlation coefficient. Results: Situational characteristics of Clinical Practice Stress(CPP) was significantly different with regards to task difficulties and efficacy self-confidence of academic self-efficacy(ASE) (r= .179, p= .003; r= .166, p= .006). Statistically significant positive relationship was founded between Personal characteristics of CPP and self-confidence of ASE (r= .239, p< .01). External regulators of CPP was significantly different according to self-confidence and task difficulties (r= .175, p= .003; r= .132, p= .028). The self-confidence and efficacy for self-confidence of ASE were a significantly correlated with Career Identity; The Career identity also was a significantly correlated with all sub-categories of CSS scale(r= -0.208, p< 0.01; r= -0.369, p< 0.01; r= -0.153, p< .011) Conclusion: This study showed positive statistical relationships associated with clinical practice stress, academic self-efficacy, and career identity as a preliminary evidence-based study for guiding education in nursing curriculum. Further research should be need for students’ future as a professional nurse.
진공 증착법을 이용한 비정질 As_2S_3박막의 제작 및 투과 스펙트럼을 이용한 광학 상수 결정
우성용,이상조,김지영,김건엽,곽종훈 嶺南大學校基礎科學硏究所 2002 基礎科學硏究 Vol.22 No.-
We fabricated the amorphous chalcogenide As_2S_3 thin film by thermal evaporation. We also measured and analysed the optical constants of refractive index, absorption coefficient, thickness of deposited amorphous As_2S_3 thin film and optical energy gap by simple measurement of transmittance spectrum.
대학생 자녀를 둔 중년여성의 자아존중감, 자녀와의 관계, SNS 이용강도가 우울에 미치는 영향
우지원,김가은,김경미,남주희,박지인,범준희,이나경,조소연,조은경,최영윤,김석선,길민지 이화여자대학교 간호과학대학 2017 이화간호학회지 Vol.- No.51
Purpose: This study aims to identify the effects of self-esteem, relationship with children, Social Networking Services(SNS) use intensity on depression in middle-aged women with college-age children, and to provide basic data for the health promotion of middle-aged women. Method: A sample of 133 middle-aged people was purposely selected from parents of university students. Questionnaires used for this study were CES-D(The Center for Epidemiological Studies-Depression Scale), Self-esteem Scale, Relationship with Children Scale, SNS Intensity Scale. The collected data was analyzed by SPSS 21 with descriptive statistics, independent t-test, ANOVAs, Pearson correlation, multiple regression. Result: Self-esteem was correlated with relationship with children (r=0.385, p<0.01) and depression (r=-0.440, p<0.01). Relationship with children was correlated with depression(r=-0.402, p<0.01). As a result, a factor having the greatest influence on the subject's depression degree was self-esteem(β=-0.335), followed by relationship with children(β=-0.274). Conclusion: The result of this study suggested that depression in middle-aged women is closely related to self-esteem and relationship with children. This result can be used in Nursing by developing the theoretical basis for education program related to enhancing self-esteem and relationship with children.
이승엽,박대원,김영아,김종렬,최정근,우희철 한국공업화학회 2000 응용화학 Vol.4 No.1
We have investigated the catalytic degradation of polystyrene(PS) in a pyrex reactor. We have used silica-alumina and zeolites(ZSM-5, SCLZ, natural zeolite, etc) as acidic catalysts. They have been ion-exchanged with hydrogen form. The products of PS degradation were analyzed by gas-chromatography(GC-MS) and distributed in a narrow range of carbon number comparing with those obtained by thermal degradation. The liquid fraction of PS degradation consisted of aromatics. The catalytic degradation of PS was dependent on active sites of catalyst pellets rather than pore size and acid density of catalysts.
김인,우영균,김용식,송석환,권순용,박수안 대한골절학회 1994 대한골절학회지 Vol.7 No.2
Authors reviewed total 28 cases of acetabular fracture with operative management followed up over 1 year. The clinical results were retrospectively analyzed with consideration of surgical prohlems and complications. The overall results were as follows: l. According to the classification by Judet and Letournel(l974), 20 cases were elementary fractures and 8 cases were associated fractures. The posterior wall fractures were most common in 9 cases. 2. Kocher-Langenbeck approach in 18 cases, ilioinguinal in 4 cases, iliofemoral in 4 cases and triradiate transtrochanteric approach in 2 cases were used. 3. The devices for internal fixation were as follows: screw only in 8 cases. plate and screw in 14 cases, plate and screw with circumferential wiring in 4 cases, wire and staple only in 1 each case. 4. The early and late complications occurred postoperatively as follows: incomplete sciatic nerve palsy 2 cases, wound infection 2 cases as early complications and posttraumatic arthritis 6 cases, avascular necrocis of femoral head 2 cases, heterotropic oscification 1 case as late complications. Two cases of sciatic nerve palsy were spontaneously recovered and 2 cases of wound infection were controlled by adequate drainage and antibiotic therapy. And then, the total hip arthroplasty were carried out for 2 cases of avascular necrosis of femoral head, and 6 cases of posttaumatic arthritis and 1 case of heterotopic ossification were under observation. 5. Postoperatively, the causes of inadequate reduction and insufficient fixation were radiographically analyzed with immediately and lastly checked plain films, of which causes in 9 cases were as follows: inappropriate approach for exposure in 4 cases, delayed operation due to major associated injury over 3 weeks in 3 cases and severe comminution in 2 cases. As a result, we reached to put an emphasis on an importatnce of preoperative planning, including the evaluation of individual fracture personality, the choice of surgical approach and the method of intemal fixation.