Cellulase-amyloglucosidase와 효모의 가스생성법에 의한 사료의 에너지가 측정에 관한 연구

김영길,이광목,김욱 東亞大學校生命資源科學大學附設 農業資源硏究所 1993 農業生命資援硏究 Vol.2 No.2

粗飼料와 濃厚飼料를 混合한 飼料의 營養價를 Cellulase와 Amyloglucosidase, 酵母를 利用한 가스 生成法에 의하여 消化率과 TDN을 測定할 수 있는 方法을 確立하고자 本 硏究를 實施한 實驗 結果는 다음과 같다. 1. Cellulose 加水分解 酵素인 Trichoderma viride cellulase의 最適 活性 pH는 4.5-4.8이었으며 pH가 5.0보다 높을 때에는 活性이 急激히 저하하였다. 2. Cellulose 加水分解시 Trichoderma viride celulase의 溫度의 의한 影響은 50℃에서 最大 活性을 나타내었으며, 40℃에서는 活性이 92%정도로 나타났다. 3. Trichoderma viride-aspergillus niger 混合, cellulase 使用時의 가스 生成量은 Trichoderma viride cellulase 使用時 가스 生成量 보다 20-30% 높게 나타났으므로 cellulose 加水分解時 Trichoderma viride-aspergillus niger 混合 cellulase를 使用하는 것이 더욱 效果的일 것으로 思料된다. 4. 포도당(X)과 가스 生成量(Y)과의 關係는 Y=6.82+165X의 回歸式과 相關係數는 0.92로서 高度의 有意性(P<0.01)이 있었다. 5. Trichoderma viride cellulase 使用時 가스 生成量(X)과 乾物 消化率(Y)과의 關係는 Y=29.9+1.13X(r=78**)의 回歸式을 나타내었고, Trichoderma viride-aspergillus niger 混合 vellulase 使用時 가스 生成量(X)과 乾物 消化率(Y)과의 關係는 Y=28.9+0.88X(r=0.82**)의 回歸式을 나타내었다. Trichoderma viride cellulase 使用時 보다 Trichoderma viride-aspergillus niger 혼합 cellulase 사용시 相關係數가 더 높았다. 6. 가스 生成量(X)과 TDN(Y)과의 關係는 Y=35.8+0.585X의 回歸式과 相關係數 0.93으로서 高度의 有意性을 나타내었고, TDN(X)과 가스 生成量(Y)과의 關係는 Y=1.68X-59.6의 回歸式을 나타내었다. The experiment was conducted to establish the gas production method by cellulase, amyloglucosidase and yeast to determine the digestibility and energy value(TDN) of feedstuffs. The results obtained were summarized as follows; 1. The highest activity of Trichoderma viride cellulase was shown at pH 4.8 and the activity of the cellulase decreased rapidly at higher pH than 5.0. 2. The highest activity of Trichoderma virie cellulase was shown at 50℃ and the activity at 40℃ was approximately 92% compared with that at 50℃. 3. The mixed celllulases of Trichoderma viride and Aspergillus nigher produced more gas than Trichoderma viride cellulase when incubated with feedstuffs and were identified to be more effectively used for gas production method. 4. The regression equation of gas production(Y) on glucose production (X) was Y=6.82+165X with significant correlation coefficient of 0.92. 5. The regression equation of DMD(Y) on gas production(X) was Y=29.9+1.13X(r=0.78) with Trichoderma viride cellulase and Y=28.9+0.88X (r=0.82) with both Trichoderma viride and Aspergillus niger cellulase. 6. The regression equation of TDN(Y) on gas production(X) was Y=35.8+0.58X with significant correlation coefficients of 0.93 and in conclusion energy value of feedstuffs can be estimated by gas production method.

CELLULASE - AMYLOGLUCOSIDASE 와 효모의 가스생성법에 의한 사료의 에너지가 측정에 관한 연구

김영길(Y . K . Kim),이광묵(K . M . Lee),김욱(U . Kim) 한국영양사료학회 1993 韓國營養飼料學會誌 Vol.17 No.3

한국인의 CCR5 유전자형은 wild homozygous이나 HIV 감염자의 LTNP 비율은 Caucasian과 유사

김성순,최병선,김은영,고운영,박근용,김태규,신영오,이주실 국립보건연구원 1998 국립보건원보 Vol.35 No.-

연 취급 근로자들의 생화학적 검사에 관한 연구

김준연,이채언,전진호,문덕환,이명철,김병수,김용완 인제대학교 1985 仁濟醫學 Vol.6 No.3

연 취급 근로자 236명과 대학생 120명을 대상으로 연 폭로에 관련되는 생물학적 지표들의 농도를 측정한 결과 PbB, Hb, PbU, ALAU에서 양군간에 유의한 차이를 시현하였으며, 연 근로자에서 PbU 및 CPU가 PbB의 증가에 따라 점증 경향을 보였다. To acquire the fundamental data to control the harmful effect by lead exposure, the authors examined several biological indicators relevant to lead exposure on 236 female lead workers in the electronics industry and 120 controls for one year from August, 1984. The obtained results were as follows; 1.The mean value of PbB, Hb, PbU, CPU and ALAU in lead workers was 24.56± 12.55㎍/100ml,13.53±0.90g/100ml, 58.54±40.00㎍/l, 39.63±26.09㎍/l and 2.33±1.3mg/l, respectively. 2.The mean value of PbB, Hb, PbU, CPU anti ALAU in controls was 16.54± 10.40㎍/100ml, 15.10±1.55g/ml, 30.74±19.05㎍/l, 34.30±26.86㎍/l, and 1.98±1.37mg/l, respectively, and the difference of the mean value of PbB, Hb, PbU and ALAU between lead workers and controls was statistically significant. 3.In lead workers, PbB and PbU, PbU and ALAU, CPU and ALAU showed significant correlation. 4.In lead workers, there showed increasing tendency of PbU and CPU as increasing PbB with statistical significance.

Nuclear Design Feasibility of the Soluble Boron Free PWR Core

Kim, J.-C 한국원자력학회 1998 Nuclear Engineering and Technology Vol.30 No.4

Lactobacilli에 의한 Bacillus cereus 의 생장억제에 관한 연구

김현욱,안영태,김용희,장영호 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.3

The growth inhibition effect of Bacillus cereus by lactobacilli has been studied. As L. acidophilus HF 04 or L. acidophilus NCFM was co-incubated with B. cereus in skim milk, the germination and sporulation of B. cereus were inhibited without affecting the growth of L. acidophilus strains. The growth curve of lactobacilli reached stationary phase after IS hours of incubation and the number of B. cereus cells decreased drastically after 10 hours of incubation. The growth of B. cereus was not observed in the skim milk medium lower than pH 4.3. The organic acids produced by lactobacilli were confirmed to inhibit the growth of B. cereus. Acetic acid was more effective growth inhibitor of B. cereus LTDM03 in skim milk medium and thermoduric spores were not germinated after 10 hours of incubation in the skim milk medium adjusted to pH 4.63 by acetic acid.

職場人의 肝炎 B바이러스 表面抗原 및 抗體에 關한 疫學的 考察

김용완,김병수,배기택,김준연,이채언,전진호,문덕환,김성천 인제대학교 1985 仁濟醫學 Vol.6 No.4

1984년 1월부터 12월까지 정기 및 채용시 신체 검사를 받고자 본 인제대학 부속 서울 및 부산 백병원 건강관리과를 찾은 남ㆍ녀 근로자 4,153명을 대상으로 혈청 HBsAg, HBsAb를 검출하고 ABO 혈액형, 매독 혈청 검사(TPHA), 간기능 검사(SCOT, SGPT)등과의 관계를 고찰하였다. To investigate the positive rate of the hepatitis B virus surface antigen (HBsAg) and its antibody (HBsAb) in the sera of healthy workers, the authors tested HBsAg and HBsAb by RPHA and PHA method respectively with the sera obtained from 4153 healthy adults (Male 3477, female 676) who were placed under the preemployment and periodic health examination in the department of Health Management of Seoul and Pusan Paik Hospital, Inje Medical College, for one year from January to December, 1984. In addition the authors studied the association of HBV infection with liver function tests (SGOT and SGPT), sero-re-activity for syphilis (TPHA) and ABO blood type. The results obtained were as follows; 1.The (overall) positive rate of HBsAg was 8.0%. This rate was significantly higher in male (8.7%) than in female (4.1%). 2.The (overall) positive rate of HBsAb was 28.6%. This rate was significantly higher in Pusan area (34.3%) than in Seoul area (25.1%). 3.The (overall) positive rate of HBsAg or HBsAb was 36.6%. This rate· was significantly higher in male (37.6%) than in female (31,2%) and in Pusan area (43.2%) than in Seoul area (32.6%). 4.The positive rates of HBsAg and HBsAb were the highest in blood type B (9.4% and 31.3%) and the lowest in blood type A (7.0% and 26.5%) but no significant differences of these positive rates among ABO blood type groups were observed. 5.The positive rate of HBsAg was significantly higher in workers with sero-reactivity for syphilis (20.0%) than in workers without sero-reactivity for syphilis (7.9%). However no significant difference of the positive rate of HBsAg or HBsAb between workers with (40.0%) and without (36.6%) sero-reactivity for syphilis was noted. 6.The Qccupied percentage of workers with HBsAg positive was significantly higher than that of workers with HBsAb positive and HBsAg or HBsAb negative. However, this phenomenon was not observed in groups of SGOT and SGPT levels less than 24 units.

부산지역 공무원과 교직원의 고혈압에 관한 역학 조사 연구

김준연,이채언,문덕환,김성천,배기택,김병수,김용완 인제대학교 1985 仁濟醫學 Vol.6 No.1

부산지역 공무원과 교직원 4,755명(남3,526명, 여1,229명)에 대한 고혈압(WHO 판정기준에 의함)유병율은 11.7%이었고, 고혈압군의 총 콜레스테롤 평균치는 200.7±44.4mg%이었으며, 그리고 고혈압군 중 비만율, 안저검사 및 E.C.G.검사의 이상 소견율은 각각 25.1%, 45.5% 및 41.5% 등이었다. Authors carried out the survey for the prevalence rate of hypertension and several factors related with the occurrence of hypertension on 4,755 numbers of public officials and school personnels in Pusan for one year from April, 1982. The obtained results were as follows: 1.The prevalence rate of hypertension among total subjects was 11.7%, and it was gradually increased with aging(p<0.05). 2.The rate of obesity (120% and over of standard body weight) in hypertensive group was higher than normotensive group (p<.05). 3.The mean concentration of total cholesterol level in hypertensive group was 200.7 ± 44.4 mg%. 4.The abnormal rate in fundus findings of hypertensive group was 45.5%, and it was gradually increased as blood pressure increased (p< .05). 5.The abnormal rate in E.C.G. findings of hypertensive group was 41.5%.

해외근로자에 있어서 산업재해 발생 원인

김용완,김병수,김성천,김준연,이채언,전진호,문덕환,배기택 인제대학교 1985 仁濟醫學 Vol.6 No.4

해외근로자 1,250명에 대한 재해원인(미국 표준연구소와 국제노동기구의 분류법에 의함)을 조사 분석하여 산업재해 예방 대책의 기초 자료를 얻고자 한 것이다. In order to obtain basic reference materiols for the estabishment of effective industrial safety programmes in oversea workers, accident causes were investigated among 1,250 injured workers, The data were analized according to the USA Standards Institute and International Labour Organization method. The results were as follows: 1.The most prevailing injuries were fractures, contusions, strains, cuts, lacerations and punctures, which were 72% of the total 1,250 cases of occupational injuries. 2.The parts of body most frequently injured were spines, upper extremities (including fingers), head and lower extremities. 3.The most responsible source of injuries were building construction, transport and power machine equipments. 4.According to the accident type, fall from elevation, over exertion, caught-in, struck-by and fall on same level were most frequently observed which were 70% of the total number of injuries. 5.According to the unsafe acts, carelessness and unsafety information were most frequently observed which were 80% of the total number of injuries. 6.Unsafety acts were mot responsiable causes of injuries, accounting for 49%. The major hazardous conditions in or about causes were approach of hazardous sites handling of hazadous matrials and carelessness of work motion. 7.Practically all oversea workers accident records could produce the causes of accident which fell into the categories. The moat hazadous conditions were unsafety acts and building construction materials were the most responsible sources of injuries. 8.There were close associations between the nature of injuries and injured body parts, the types and of causes injuries, unsafety acts and kinds of work among injured workers.

抗生物質의 製劑學的 硏究 : (3) EUDRAGIT을 이용한 암피실린 마이크로캅셀의 제조 및 평가 (3) The Preparation and Characteristics of Eudragit-Ampicillin Microcapsules

金基璟,金度勳,尹美玉,張禎允,金英林,崔先玉,李敬熙,林秀庭,金壽億 국립보건연구원 1992 국립보건원보 Vol.29 No.2