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逆流浸降 電流法에 依한 돼지의 X-, Y-精子分離 關한 硏究
李用斌,呂政秀,鄭用基,尹種擇,徐國聖 서울大學校 農科大學 1980 서울대농학연구지 Vol.5 No.2
This experiment was carried out to separate X- and Y- bearing sperm of boar semen from 4 boars raising at the College of Agriculture, Seoul National University. The boar semen collected was treated by the counter streaming sedimentation and galvanization. The results obtained were as follows; Average appearance of B-body from control was 45.2%, when the experiment was performed with counter streaming sedimentation alone appearance of B-body from top and bottom fraction were 59.4% and 36.3%, respectively. The frequency of appearance of B-body from 30(24%) and 40(19.6%) minutes among treatments were statistically significant differences. Appearance of B-body from anode was 54% and from cathode was 37.9% after counter streaming sedimentation and galvanization. The difference between anode and cathode was significant differences at the 30(14.7%), 40(19.4%) and 50(19.9%) minutes. The frequency of appearance of B-body from counter streaming galvanization was significantly higher than that from counter streaming sedimentation. Therefore, the result from counter streaming sedimentation(30 minutes) and galvanization(30 minutes) was the most useful.
재래돼지(Korea Native Pigs) 특이 genetic marker와 육질 연관성 분석
김철욱,여정수,조광근,진상근,오명곤,박준규,권은정,홍연희,김지현,이보경,박다혜,김재우,이지홍 한국동물자원과학회 2001 한국축산학회지 Vol.43 No.6
본 연구는 재래돼지 특이적인 marker를 개발하고 이들 marker와 육질과의 연관성을 규명하기 위하여 실시하였다. 실험동물로 재래돼지와 랜드레이스 각각 30두씩 총 60두를 사용하였으며, 품종 특이 marker를 찾기 위하여 4번과 7번 염색체에 존재하는 총 60개의 microsatellite를 이용하여 PCR (polymerase chain reaction)을 수행하였다. PCR 증폭은 형광물질 Fam과 Hex, Ned로 표지된 한쌍의 microsatellite primer를 이용하였으며, 형광물질로 표식된 PCR 산물은 Genetic Analyzer ABI 310으로 전기영동하여 대립 유전자를 규명하고 통계 분석하였다. 대립 유전자에 대한 통계 분석 결과 총 60개의 microsatellite중 27개가 다양한 유전적 다형현상을 보이는 대립 유전자가 증폭되었으며 (p<0.05), 특히 SW1364와 SW445, SW1369, SWR773 microsatellite의 증폭 산물에서 재래돼지와 랜드레이스 두 품종간을 구분할 수 있는 품종 특이적인 대립 유전자를 확인하였다 (p<0.01). 또한 육질에 영향을 미치는 유전적 marker를 얻기 위해 60두 전체에 대한 육색, 지방색, 지방산 조성, 콜레스테롤 함량, 연도와 경도에 대한 육질 분석을 실시하여 육질 형질과 marker와의 연관성을 분석한 결과 육질 형질에 영향을 미치는 28개 marker를 확인하였으며, 육색과 지방색에 연관된 4개, 조직감과 연관된 10개의 marker를 확인하였다. 이렇게 선발된 marker들은 고급육 생산을 위한 돼지의 유전적 개량을 위하여 유용하게 이용될 수 있다. This study was conducted to develop Korean native pig-specific DNA markers and analyze its relativity with meat quality using 30 of Korean native pigs and Landraces, respectively. To find species-specific DNA markers, we selected and tested a total of 60 microsatellite markers on the 4 and 7 chromosome using polymerase chain reaction (PCR). PCR amplification was carried out by microsatellite primer pairs labeled with fluorescent Fam, Hex and Ned. The fluorescent labeled PCR products were electrophoresed with Genetic Analyzer ABI 310 followed by the allelic and statistical analysis. According to the allelic frequency annalysis, of the 60 microsatellite markers 27 were preduced with a variety of genetic polymorphic alleles (p<0.05). Especially, alleles of SW1364, SW445, SW1369 and SWR773 microsatellite markers were identified to be species-specific markers between two breeds (p<0.01). To analyze the meat quality we determined the meat color, fat color, fat composition, cholesterol contents and hardness, and compared these characteristics with the alleles of 36 species-specific markers in all pigs. Of the 60 microsatellite markers 28 were found to be related with meat quality; 18 for meat and fat color, 7 for fatty acid, 4 for cholesterol contents and 10 for shear force and hardness. These selected markers will be useful in the genetic improvement of pigs for an excellent meat quality.
전기영동과 산알카리처리 결합방법에 의한 돼지정액의 X 와 Y 정자 분리에 관한 연구
이용빈,서국성,여정수,정용기 ( Y . B . Lee,G . S . Suh,J . S . Yeo,Y . K . Chung ) 한국축산학회 1980 한국축산학회지 Vol.22 No.4
The combination of the acid-alkali method and eletrophoresis for X-and Y-sperm separation of boar semen was performed We used lactic acid and sodium bicarbonate in acid-alkali method, and electrophoresed at 2.5V and 200 μA. The results obtained were summarized as follows : 1. The frequency of appearance of B-body from alkali treatment of whole semen was significantly higher than that from acid treatment. 2. The frequency of appearance of B-body from alkali treatment at the anode electrode was significantly higher compared with that from acid treatment at the same electrode, while that from acid treatment at the cathode was slightly higher than that from alkali treatment at the same electrode. 3. According to time of treatment, Appearance of B-body from alkali treatment at the anode after electrophonesis was decreased, and the result from acid treatment at the same condition was the opposite, while appearance of B-body from alkali treatment at the cathode after electrophoresis was not changed and that from acid treatment at the same condition was increased. 4. Appearance of B-body from alkali treatment at the anode for 5-10minutes showed the most useful result which was higher than the result (51.9%) from electrophoresis alone by 3 %.
Hong, B B,Choi, L,Jung, Y C,Hwang, S W,Cho, K H,Yeo, K H,Kim, D,Jin, G Y,Park, D,Song, S H,Lee, Y Y,Son, M H,Ahn, D IEEE 2010 IEEE TRANSACTIONS ON NANOTECHNOLOGY Vol.9 No.6
<P>We report the random telegraph noise observed in gate-all-around (GAA) PMOS silicon nanowire FETs (SNWFETs) with the radius of 5 nm, at various temperatures (s) down to 4.2 K. From the -dependence of the capture/emission time, we obtain the energy and the charging status of the trap states. The gate bias dependence and the -dependence of the scattering coefficient-mobility product extracted from the relative fluctuation amplitude of the drain current are consistent with the fact that the surface roughness scattering is dominant in GAA PMOS SNWFETs.</P>
한우의 일당 증체량에 연관된 DNA Marker 의 규명
여정수,박노형,김재우,이문연,장태경 한국축산학회 1999 한국축산학회지 Vol.41 No.4
DNA markers related to daily gain from 287 heads( 173 heads of bull and 114 heads of steer) of Hanwoo were found with distinct 3 kinds at 4.6, 3.6 and 2.8kb using DNA fingerprinting with M13/HaeIII and 2 kinds at 5.6 and 2.1kb with M13/Hinfl. It was evidently considered that 3 specific markers influenced to grow in Hanwoo, negative effect in 4.bkb and positive in o hers(3.6 and 2.8kbs) for daily gain and especially single effect at 3.bkb for bulls and combined effect of 4.6 arid 2.8 markers for steers were distinctive to daily gain with M13/HaeIII. Pedigreed pattern of specific markers with M13/HaeⅢ were observed with DNA bands at 2.8 and 3.bkb were dependent on sire. Under results of this research culling of 4.bkb marker and selection of 3.bkb marker in Hanwoo population can be expected to improve above 35㎏ at market body weight.
한국재래돼지의 경제형질에 연관된 AFLP marker 의 분석
여정수,김재우,이지홍,장태경,박영애 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.3
DNA markers related to daily gain and backfat thickness of Korean Native Pig which were sustained as different characteristics from other breeds in DNA constitution were identified by AFLP (amplified fragment length polymorphism). Specific marker was found in lower group of daily gain showing greater influence in males and transmitted with dominant inheritance. DNA marker related to daily gain was tremsmatted to all of progenies from parent. In backfat thickness, marker related to thinner group was inherited only from sire to all progenies, not from dam. These DNA markers with different effects by sex will be useful tools to improve important economic traits in Korean Native Pig.
Kim, Y.,Yeo, H.,You, N. H.,Jang, S.,Ahn, S.,Jeong, K. U.,Lee, S.,Goh, M. Royal Society of Chemistry 2017 Polymer chemistry Vol.8 No.18
<P>To develop advanced thermally conductive epoxy resins with liquid crystallinity, three novel liquid crystalline epoxies (LCEs) were synthesized via the substitution of phenylcyclohexyl (PCH) mesogenic moieties into the 2,5 positions of diglycidyl terephthalate. Mesomorphic properties of LCEs were evaluated by differential scanning calorimetry (DSC), polarized optical microscopy (POM), and X-ray diffraction (XRD). All LCEs exhibited an enantiotropic smectic phase on heating and cooling. Remarkably, the smectic phase with a wide temperature range of 98-145 degrees C was observed for the eutectic mixtures of a family of stable smectic LCEs. The selection of optimum curing agents, compositions of LCEs and curing agents, and curing conditions were determined based on physical, chemical, and thermal properties of LCEs and curing agents. The thermally cured LCEs at the LC phase exhibited a high thermal conductivity of 0.4 W m(-1) K-1.</P>
6차 교육과정에 따른 고등학교 화학II 교과서에서의 극성 및 무극성 분자의 전기적 성질 실험의 비교 분석 및 개선
여수동,이무상,여환진,이광필,이우붕,배준웅,천영숙 慶北大學校 師範大學 科學敎育硏究所 1998 科學敎育硏究誌 Vol.22 No.-
The purpose of this study is to comparatively analyze and improve the Electronic properties of Polar and Nonpolar Molecules in the High School Chemistry II Textbooks on the Basis of 6th Education Curriculum. From the results of Experiment about the Electronic properties of Polar and Nonpolar Molecules, we have found some problems and have suggested the solution of these problems.
6차 교육과정에 의거 개정된 8종의 중학교 1학년 교과서 중에서 녹는점 측정 실험의 비교 분석 및 개선
이무상,여수동,여환진,이우붕,배준웅,이광필,남상일,최윤정 경북대학교 과학교육연구소 1997 科學敎育硏究誌 Vol.21 No.-
The purpose of this study is to comparatively analyze and improve the melting point experiment in the first grade middle school science textbooks on the basis of the 6th revised curriculum. From the results of the melting point experiment of paradichlorobenzene, we have found two problems on the measurement of melting point and have suggested the solution of these problems.