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Xue-Ke Gao,Shuai Zhang,Jun-Yu Luo,Chun-Yi Wang,Li-Min Lü,Li-Juan Zhang,Xiang-Zhen Zhu,Li Wang,Jin-Jie Cui 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.3
Odorant-binding proteins (OBPs) play a significant role in the olfactory signal transduction of insects and help them locate hosts, oviposition sites, and mating partners. The sap-sucking insect, Aphis gossypii Glover (Homoptera: Aphididae), is a destructive cosmopolitan pest and yet the molecular mechanisms by which A. gossypii perceives pheromones and host volatiles remain unknown. In this study, we identified and characterized 6 OBPs, using the A. gossypii RNA-seq transcriptome dataset previously constructed in our laboratory. Real-time PCR indicated specific expression patterns of the 6 genes, which had different levels of expression based on development stage, tissue, morph, and life cycle. In addition, binding specificities of the 6 proteins investigated using the ligand-binding assays showed that all 6 OBPs exhibited high binding affinities towards Phlorizin dehydrate while AgosOBP3 and AgosOBP4 had strong affinity to beta-ionone and AgosOBP8 displayed higher binding affinities for Nerolidol and Cis-3-hexenyl acetate compared to other OBPs.
2D Geometric Constraint Optimum Solving Based on Problem Decomposition
Xue-Yao Gao,Chun-Xiang Zhang,Zhi-Mao Lu 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.1
Constraint solving is widely applied to many fields including computer aided design, 2 dimension (2D) model design and computer aided manufacturing. Geometric constraint solution is a difficult problem because there are a large number of entities and related parameters in 2D sketches. In this paper, a new method which decomposes geometric constraint relations based on entity-parameter graphs is proposed for reducing the size of constraint solution. A geometric constraint problem is decomposed into many independent sub-problems. Then, particle swarm optimization algorithm is used to solve constraint equations in each sub-problem. Solutions of all sub-problems are integrated to obtain the original problem’s solution. In experiments, the proposed method is applied to HUST-CAID system. Experimental results show that the method can effectively solve 2 dimension geometric constraints.
Xue Shuang,Gao Shanshan,Cao Yizhuo,Gao Tian,Yin Se,Zhang Yong-lei,Lu Ruixue,Zhang Kunpeng 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2
Elongases (ELO) is a large class of enzymes involved in the development and reproduction of insects. Although some ELOs have been isolated and characterized in a small number of insects, little is known about the specific function of ELOs in insects, especially in the regulatory mechanism of ELOs. In this study, the function and regulation of TcELO were investigated in Tribolium castaneum. Spatiotemporal expression profiling indicated that TcELO expressed in all development stages, and high expressed in the head, epidermis, and ovary of T. castaneum. By comparing the transcriptome profiles of RNAi-treated larvae (dsTcELO) and control larvae of T. canstaneum using RNA sequencing, 320 differentially expressed genes (DEGs) were identified from the ds-ELO-treated group. Compared with the control group, five genes including cytc2, sfxn3 and H2A.V were significantly up-regulated in the dsTcELO group, suggesting that TcELO may be involved in the apoptosis of insect biological cells to control the growth and development system of insects. However, NAT was significantly down-regulated, indicating that it cooperates with TcELO to control the reproductive process of insects. Knockdown of TcELO also significantly up-regulated related genes in environmental adaptation pathways, such as COXIV, COXc6, and ATPs, which suggested that these genes may regulate TcELO to involve in epidermal function of insects and thier adaptability to the environment. These results provide new insights into the regulatory mechanism of TcELO involved in insect physiology, and can provide theoretical support for the control of agricultural pests.
Retrieve CAD Model Based on Face Matching Sequence
Gao Xue-Yao,Li Hui-Nan,Hu Ru,Zhang Chun-Xiang 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.3
A new model retrieval method based on face matching sequence is proposed in this paper. Attribute adjacent graph is used to describe two faces’ geometry similarity and topological relationship in CAD model. According to the difference of edge numbers, similarities between two models’ faces are computed and face similarity matrix is constructed. Ant colony algorithm (ACA) is applied to obtain an optimal sequence of matching faces between two models. Accumulate similarity values of optimal matching faces to calculate two models’ similarity. Experimental results show that this method can evaluate two CAD models’ shape difference effectively.
New Triterpenoids from the Fruits of Schisandra wilsoniana and Their Biological Activities
Gao, Xue-Mei,Li, Yun-Qi,Shu, Li-Dan,Shen, Yan-Qiong,Yang, Li-Ying,Yang, Liu-Meng,Zheng, Yong-Tang,Sun, Han-Dong,Xiao, Wei-Lie,Hu, Qiu-Fen Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.3
Investigation of an organic extract of the fruits Schisandra wilsoniana led to the isolation of two new highly oxygenated nortriterpenoids, named schilancidilactones V-W (1-2). Their structures were elucidated by spectroscopic evidence. Compounds 1-2 feature a double bond between C-7 and C-8 compared with related known nortriterpenoids isolated from the genus Schisandra. Compounds 1 and 2 were tested for their anti-HIV-1 activities and cytotoxicity. The results revealed that compounds 1 and 2 showed moderate anti-HIV-1 activities with $EC_{50}$ 3.05 and 2.87 ${\mu}g/mL$, respectively, and compound 1 showed high cytotoxicity against KB and MDA-MB-231 cell with $IC_{50}$ values of 3.18 and 5.22 ${\mu}M$, respectively.
Name and Maintain Topological Faces in Rotating and Scanning Features
Gao Xue-Yao,Li Jia-Qi,Guo Hao,Gao Yun-Feng,Liu Yu-Hong 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.3
Features created in rotating and scanning operations are very complex. Naming and identifying their topological faces is an important problem in CAD fields. In this paper, a new method of coding topological faces in rotating and scanning features is proposed. Firstly, contour segments are numbered. Secondly, an angle between contour segment and rotating axis is computed. Thirdly, all topological faces are named based on contour segments’ numbers, rotating axis and other information. When a face splits and several subfaces merge, a method of processing their codes is given. The proposed method is applied to HUST-CAID feature modeling system. Experimental results show that it can name and identify topological faces effectively in operations.
Compute Similarity of CAD Models Based on Bipartite Graph
Xue-Yao Gao,Chun-Xiang Zhang,Zhi-Mao Lu 보안공학연구지원센터 2015 International Journal of Signal Processing, Image Vol.8 No.2
Model retrieval is widely applied to many fields including computer aided design, feature modeling and computer aided manufacturing. In order to retrieve a satisfied CAD model from a large model library, a new method to compute the similarity between two CAD models is presented in which a bipartite graph is used to match source faces with target faces. The number of edges in faces is extracted from CAD models. Based on the number of edges in faces, the similarity between source face and target face is computed. The maximum matching weight is calculated by KM algorithm. Then, the similarity between two CAD models is gotten. Several CAD models are given and their similarities are computed. Experimental results show that the method can evaluate the similarity between source CAD model and target CAD model efficiently.