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RISS 인기검색어
- 검색키워드
- 저자 : Xiu Mei Dai (검색결과 3 건)
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Xiu Mei Dai,Ru Hong Xu,Jun Lu,Fang Li,Jia Na Li,You Rong Chai 한국유전학회 2008 Genes & Genomics Vol.30 No.5
In the introgression of the distinguished powdery mildew resistance from Haynaldia villosa to wheat, a focus task is to develop applicable molecular markers for tracing alien genetic substances and breeding selection. In an attempt to breed wheat breeding lines combining powdery mildew resistance of wheat stock 010714 conferred by alien 6V chromosome from H. villosa with thermo- photo-sensitive genic male sterility (TGMS) from wheat line C050S, a molecular marker RM874 was identified in PCR amplification using RAPD primer S2018. Batched sequencing indicated that the band contained large amounts of equal-length homologues amplified from Ty3-gypsy retrotransposon-like repetitive regions. Based on comparative cloning and bioinformatic analysis, it was converted into a more reproducible sequence characterized amplified region (SCAR) marker SM142. In wheat genetic background, the marker is 6V-specific and linked to powdery mildew resistance. The linkage of the marker is further verified in F5 generation. The approach of transforming repetitive regions-derived RAPD band into a specific SCAR marker can be referred for SCAR marker development of other genes. GISH (Genome in situ hybridization) analysis of 010714 and F7 translocation lines indicated that the resistance gene is conferred by the short arm of 6V.
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Hybridization Using a New Male-sterile Germplasm as the Female Parent in Chinese Jujube
Jiu-rui Wang,Xiu-mei Cui,Li Dai,Ping Liu,Jin Zhao,Meng-jun Liu 한국원예학회 2015 원예과학기술지 Vol.33 No.3
This study identified a new male-sterile germplasm of Chinese jujube, named male-sterile No. 2 (JMS2), and achieved controlled hybridization using that germplasm as the female parent. The anthers of JMS2 before flower bud opening became shrunken, dingy yellow and much smaller than normal ones, and they changed to brown after anthesis. No pollen was observed in anthers of JMS2 and its male-sterile trait remained stable over different years. A total of 1,642 fruits were obtained from ten intra- and interspecific cross combinations via controlled hybridization from 2008 to 2012 using JMS2 as the female parent. Of those, 27.3% produced seeds, on average (0-72.6%). The rate of fruit with seed (RFS) was significantly different between cross combinations or years. Compared to other cross combinations, the RFS in the combination of JMS2 × ‘Xingguang’ (a Chinese jujube cultivar with high resistance to jujube witches’ broom disease) and JMS2 × ‘Xing16’ (a sour jujube genotype) remained high in different years and reached means of 48.7 and 58.1%, respectively. Finally, 150 plantlets were regenerated from immature embryos, and 51 of them were randomly selected and identified to be authentic hybrids using amplified fragment length polymorphism (AFLP) markers. This is the first report of hybrids obtained from a cross between Chinese jujube and sour jujube.
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Hybridization Using a New Male-sterile Germplasm as the Female Parent in Chinese Jujube
Wang, Jiu-rui,Cui, Xiu-mei,Dai, Li,Liu, Ping,Zhao, Jin,Liu, Meng-jun Korean Society of Horticultural Science 2015 원예과학기술지 Vol.33 No.3
This study identified a new male-sterile germplasm of Chinese jujube, named male-sterile No. 2 (JMS2), and achieved controlled hybridization using that germplasm as the female parent. The anthers of JMS2 before flower bud opening became shrunken, dingy yellow and much smaller than normal ones, and they changed to brown after anthesis. No pollen was observed in anthers of JMS2 and its male-sterile trait remained stable over different years. A total of 1,642 fruits were obtained from ten intra- and interspecific cross combinations via controlled hybridization from 2008 to 2012 using JMS2 as the female parent. Of those, 27.3% produced seeds, on average (0-72.6%). The rate of fruit with seed (RFS) was significantly different between cross combinations or years. Compared to other cross combinations, the RFS in the combination of JMS2 ${\times}$ 'Xingguang' (a Chinese jujube cultivar with high resistance to jujube witches' broom disease) and JMS2 ${\times}$ 'Xing16' (a sour jujube genotype) remained high in different years and reached means of 48.7 and 58.1%, respectively. Finally, 150 plantlets were regenerated from immature embryos, and 51 of them were randomly selected and identified to be authentic hybrids using amplified fragment length polymorphism (AFLP) markers. This is the first report of hybrids obtained from a cross between Chinese jujube and sour jujube.
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