A Novel Molecular Grading Model: Combination of Ki67 and VEGF in Predicting Tumor Recurrence and Progression in Non-invasive Urothelial Bladder Cancer

Chen, Jun-Xing,Deng, Nan,Chen, Xu,Chen, Ling-Wu,Qiu, Shao-Peng,Li, Xiao-Fei,Li, Jia-Ping Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Purpose: To assess efficacy of Ki67 combined with VEGF as a molecular grading model to predict outcomes with non-muscle invasive bladder cancer (NMIBC). Materials: 72 NMIBC patients who underwent transurethral resection (TUR) followed by routine intravesical instillations were retrospectively analyzed in this study. Univariate and multivariate analyses were performed to confirm the prognostic values of the Ki67 labeling index (LI) and VEGF scoring for tumor recurrence and progression. Results: The novel molecular grading model for NMIBC contained three molecular grades including mG1 (Ki67 $LI{\leq}25%$, VEGF $scoring{\leq}8$), mG2 (Ki67 LI>25%, VEGF $scoring{\leq}8$; or Ki67 $LI{\leq}25%$, VEGF scoring > 8), and mG3 (Ki67 LI > 25%, VEGF scoring > 8), which can indicate favorable, intermediate and poor prognosis, respectively. Conclusions: The described novel molecular grading model utilizing Ki67 LI and VEGF scoring is helpful to effectively and accurately predict outcomes and optimize personal therapy.

Modeling, Preparation, and Elemental Doping of Li₇La₃Zr₂O₁₂ Garnet-Type Solid Electrolytes: A Review

Cao, Shiyu,Song, Shangbin,Xiang, Xing,Hu, Qing,Zhang, Chi,Xia, Ziwen,Xu, Yinghui,Zha, Wenping,Li, Junyang,Gonzale, Paulina Mercedes,Han, Young-Hwan,Chen, Fei The Korean Ceramic Society 2019 한국세라믹학회지 Vol.56 No.2

Recently, all-solid-state batteries (ASSBs) have attracted increasing interest owing to their higher energy density and safety. As the core material of ASSBs, the characteristics of the solid electrolyte largely determine the performance of the battery. Thus far, a variety of inorganic solid electrolytes have been studied, including the NASICON-type, LISICON-type, perovskite-type, garnet-type, glassy solid electrolyte, and so on. The garnet Li₇La₃Zr₂O₁₂ (LLZO) solid electrolyte is one of the most promising candidates because of its excellent comprehensively electrochemical performance. Both, experiments and theoretical calculations, show that cubic LLZO has high room-temperature ionic conductivity and good chemical stability while contacting with the lithium anode and most of the cathode materials. In this paper, the crystal structure, Li-ion transport mechanism, preparation method, and element doping of LLZO are introduced in detail based on the research progress in recent years. Then, the development prospects and challenges of LLZO as applied to ASSBs are discussed.

Study on Multi-Field Coupled Evolution Mechanism of Laser Irradiated 40Cr Steel Quenching Process Based on Phase Change Induced Plasticity

Chang Li,Hexin Gao,Xinxue Chen,Zhaotai Liu,Xing Han 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.8

40Cr is essential to steel for the manufacture of hardened gears, and optimizing its laser quenching process will be of greatsignificance for gear wear and life extension. However, the complete reliance on the experimental trial-and-error approachdoes not effectively reveal the mechanism of the evolution of the laser quenching process in disk laser. Numerical simulationprovides an effective way to obtain the transient evolution of the laser quenching process. In this paper, the temperaturechanges physical parameters of the quenched material are calculated based on the CALPHAD method. Establish a thermoelastic–plastic multi-field coupling model for the laser hardening process of the disk laser 40Cr gear steel. Perform thenumerical calculations on the transient temperature, martensite phase fraction, and the transformation-induced plasticitystress evolution of the 40Cr laser hardening process. Focus on the mechanism of the mutual coupling between phase changebehavior and plastic strain. Calculations show that there is heat accumulation during quenching, with a peak temperatureof 1448 K. Plastic stress has a “hump” distribution, with a peak value of 834 MPa, which is 1.25 mm from the center ofthe focal point. The experimental analyses of the 40Cr gear steel quenching microstructure and hardness distribution stateby the Axioskop2 scanning electron microscope, the KEYENCE VH-Z100R super depth of field 3D microscope, and theQ10M microhardness tester reveal the mechanical behavior of the material and the phase hardening law during the quenchingprocess. That provided an essential theoretical basis for effective prediction on quenching residual stress and optimizingindustrial production parameters.

Effect of Bee Venom Acupuncture on Oxaliplatin-Induced Cold Allodynia in Rats

Lim, Bong-Soo,Moon, Hak Jin,Li, Dong Xing,Gil, Munsoo,Min, Joon Ki,Lee, Giseog,Bae, Hyunsu,Kim, Sun Kwang,Min, Byung-Il Hindawi Publishing Corporation 2013 Evidence-based Complementary and Alternative Medic Vol.2013 No.-

<P>Oxaliplatin, a chemotherapy drug, often leads to neuropathic cold allodynia after a single administration. Bee venom acupuncture (BVA) has been used in Korea to relieve various pain symptoms and is shown to have a potent antiallodynic effect in nerve-injured rats. We examined whether BVA relieves oxaliplatin-induced cold allodynia and which endogenous analgesic system is implicated. The cold allodynia induced by an oxaliplatin injection (6 mg/kg, i.p.) was evaluated by immersing the rat's tail into cold water (4°C) and measuring the withdrawal latency. BVA (1.0 mg/kg, s.c.) at Yaoyangguan (GV3), Quchi (LI11), or Zusanli (ST36) acupoints significantly reduced cold allodynia with the longest effect being shown in the GV3 group. Conversely, a high dose of BVA (2.5 mg/kg) at GV3 did not show a significant antiallodynic effect. Phentolamine (<I><I>α</I></I>-adrenergic antagonist, 2 mg/kg, i.p.) partially blocked the relieving effect of BVA on allodynia, whereas naloxone (opioid antagonist, 2 mg/kg, i.p.) did not. We further confirmed that an intrathecal administration of idazoxan (<I><I>α</I></I><SUB>2</SUB>-adrenergic antagonist, 50 <I><I>μ</I></I>g) blocked the BVA-induced anti-allodynic effect. These results indicate that BVA alleviates oxaliplatin-induced cold allodynia in rats, at least partly, through activation of the noradrenergic system. Thus, BVA might be a potential therapeutic option in oxaliplatin-induced neuropathy.</P>

Remote sensing of early-stage green tide in the Yellow Sea for floating-macroalgae collecting campaign

Xing, Qianguo,Wu, Lingling,Tian, Liqiao,Cui, Tingwei,Li, Lin,Kong, Fanzhou,Gao, Xuelu,Wu, Mengquan Elsevier 2018 Marine pollution bulletin Vol.133 No.-

<P><B>Abstract</B></P> <P>The world's largest green tide originated from the Jiangsu Shoal of the Yellow Sea was due to fast reproduction of floating green macroalgae (<I>Ulva prolifera</I>). It brought significant impacts on marine environment and ecosystem in the Yellow Sea. In this study, we examined the expansion of green tide from the Jiangsu Shoal during the period from 29 April to 25 June 2016. Using high-resolution satellite images, we revealed a declined growth rate during the northward drifting of early-stage green tide for the first time, i.e., the green tide had higher growth rate (up to 25% per day) in the turbid waters of the Jiangsu Shoal in May and a lower growth rate (low to 3% per day) in the relatively clear waters in the middle of the western Yellow Sea in June, which suggests that water clarity might not be the key factor controlling the growth rate of the floating macroalgae in the surface waters under natural conditions. The high growth rate led to shortened time windows for controlling the green tide by employing macroalgae collecting campaigns at the initial sites of the green tide, which was no more than 14 days in the 2016 case.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Using high-resolution satellite image for detecting early-stage green tide </LI> <LI> Found changing growth rate of green tide </LI> <LI> Assessed the countermeasure of collecting floating-macroalgae at the initial sites </LI> </UL> </P>

The Correlation between Thyrotropin and Dyslipidemia in a Population-based Study

Li Lu,Beibei Wang,Zhongyan Shan,Fengwei Jiang,Xiaochun Teng,Yanyan Chen,Yaxin Lai,Jiani Wang,Haibo Xue,Sen Wang,Chenyan Li,He Liu,Ningna Li,Jiashu Yu,Liangfeng Shi,Xin Hou,Qian Xing,Xue Bai,Weiping Te 대한의학회 2011 Journal of Korean medical science Vol.26 No.2

This study investigated the relationship between serum thyrotrophin levels and dyslipidemia in subclinical hypothyroid and euthyroid subjects. A total of 110 subjects with subclinical hypothyroidism and 1,240 euthyroid subjects enrolled in this study. Patients with subclinical hypothyroidism had significantly lower high density lipoprotein cholesterol (HDL-C) levels than those who were euthyroid. The lipid profiles were each categorized and mean thyrotrophin levels were higher in subjects in the dyslipidemia subclasses than subjects in the normal subclasses. Thyrotrophin was positively associated with serum triglyceride and negatively associated with serum HDL-C in women. Thyrotrophin was also positively associated with total cholesterol (TC) in the overweight population along with TC and LDL-C in overweight women. In the euthyroid population, thyrotrophin was positively associated with TC in the overweight population. In conclusion, serum thyrotrophin was correlated with dyslipidemia in subclinical hypothyroid and euthyroid subjects; the correlation was independent of insulin sensitivity.

Mapping of QTLs controlling content of fatty acid composition in rapeseed (Brassica napus)

Xing Ying Yan,Jia Na Li,Rui Wang,Meng Yan Jin,Li Chen,Wei Qian,Xin Na Wang,Lie Zhao Liu 한국유전학회 2011 Genes & Genomics Vol.33 No.4

The improvement of fatty acid composition is one of the major goals of breeding in rapeseed (Brassica napus). The aim of this study was to provide more information on the genetic determination of fatty acid composition by investigating quantitative trait loci (QTLs). The study was based on two-year of field trials (in 2006 and 2007) with a population of recombinant inbred lines (RILs), which originated from a cross between GH06 and P174. The level of erucic acid (C22:1) was significantly negatively correlated with those of palmitic acid (C16:0), oleic acid (C18:1), linoleic acid (C18:2), linolenic acid (C18:3), and eicosenoic acid (C20:1) in both years. A total of 40 QTLs for six fatty acids were detected and most of them were clustered on linkage groups N8, N9, and N13. These results suggested strongly that there were significant correlations between the levels of fatty acid components and would be useful for the future improvement of breeding programs focused on fatty acids in rapeseed.

Pituitary P62 deficiency leads to female infertility by impairing luteinizing hormone production

Li Xing,Zhou Ling,Peng Guiliang,Liao Mingyu,Zhang Linlin,Hu Hua,Long Ling,Tang Xuefeng,Qu Hua,Shao Jiaqing,Zheng Hongting,Long Min 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

P62 is a protein adaptor for various metabolic processes. Mice that lack p62 develop adult-onset obesity. However, investigations on p62 in reproductive dysfunction are rare. In the present study, we explored the effect of p62 on the reproductive system. P62 deficiency-induced reproductive dysfunction occurred at a young age (8 week old). Young systemic p62 knockout (p62 -/- ) and pituitary-specific p62 knockout (p62 flox/flox αGSU cre ) mice both presented a normal metabolic state, whereas they displayed infertility phenotypes (attenuated breeding success rates, impaired folliculogenesis and ovulation, etc.) with decreased luteinizing hormone (LH) expression and production. Consistently, in an infertility model of polycystic ovary syndrome (PCOS), pituitary p62 mRNA was positively correlated with LH levels. Mechanistically, p62 -/- pituitary RNA sequencing showed a significant downregulation of the mitochondrial oxidative phosphorylation (OXPHOS) pathway. In vitro experiments using the pituitary gonadotroph cell line LβT2 and siRNA/shRNA/plasmid confirmed that p62 modulated LH synthesis and secretion via mitochondrial OXPHOS function, especially Ndufa2, a component molecule of mitochondrial complex I, as verified by Seahorse and rescue tests. After screening OXPHOS markers, Ndufa2 was found to positively regulate LH production in LβT2 cells. Furthermore, the gonadotropin-releasing hormone (GnRH)-stimulating test in p62 flox/flox αGSU cre mice and LβT2 cells illustrated that p62 is a modulator of the GnRH-LH axis, which is dependent on intracellular calcium and ATP. These findings demonstrated that p62 deficiency in the pituitary impaired LH production via mitochondrial OXPHOS signaling and led to female infertility, thus providing the GnRH-p62-OXPHOS(Ndufa2)-Ca 2+ /ATP-LH pathway in gonadotropic cells as a new theoretical basis for investigating female reproductive dysfunction.

Contribution of Lipids in Honeybee (Apis mellifera) Royal Jelly to Health

Xing-An Li,Chaoqun Huang,Yun-Bo Xue 한국식품영양과학회 2013 Journal of medicinal food Vol.16 No.2

Honeybee (Apis mellifera) royal jelly (RJ) has a long history in human medicine because of its health-protecting properties. To develop a fundamental and comprehensive understanding of lipids in RJ, this article reviews the available literature on lipid compounds identified from RJ extracts and in vitro pharmacological effects of 10-hydroxy-2-decenoic acid in RJ and other closely related compounds, some of which are also identified as lipid compounds in RJ. Overall, the lipids in RJ are composed of mostly (aliphatic) fatty acids, almost all of which are present as free fatty acids and scarcely any as esters. Most fatty acids in RJ are medium-chain fatty acids, whether hydroxylated in terminal and/or internal positions, terminated with mono- or dicarboxylic acid groups, and saturated or monounsaturated at the 2-position. Besides these fatty acids, lipids in RJ contain sterols in minor amounts. Lipids in RJ are useful as preventive and supportive medicines with functionalities that include potential inhibitors of cancer growth, immune system modulators, alternative therapies for menopause, skin-aging protectors, neurogenesis inducers, and more. Taken together, the evidence suggests that health-protecting properties of RJ can be, in part, ascribed to actions of lipids in RJ.

Hydrogenation of dimethyl 2,5-furandicarboxylate to dimethyl tetrahydrofuran-2,5-dicarboxylate over Ru/HY

Li Chengfeng,Lyu Qishen,Li Zheng,Yang Shuliang,Sun Yong,Tang Xing,Zeng Xianhai,Lin Lu 한국화학공학회 2023 Korean Journal of Chemical Engineering Vol.40 No.9

The hydrogenation of biomass-based furan compounds is an important step for the catalytic valorization of biobased chemicals. In this work, Ru-based catalysts supported on different supports, such as HY, MgO and Amberlyst-15, were prepared and estimated for the hydrogenation of dimethyl 2,5-furandicarboxylate (FDMC). Among them, Ru/HY provided a desirable yield of 99.4% for tetrahydrofuran-2,5-dicarboxylic acid dimethyl ester (THFDMC) under 90 °C and 3 MPa H2. Based on catalyst characterization, Ru/HY could offer more surface Ru0 species and carbon radical intermediates than other catalysts, which could largely favor the activation of hydrogen and then promote the hydrogenation of FDMC over Ru/HY.