MicroRNA-590-5p Regulates Proliferation and Invasion in Human Hepatocellular Carcinoma Cells by Targeting TGF-beta RII

Xiaofeng Jiang,De Chen,Guangyang Xiang,Yezeng Wang,Lei Zhang,Xuewei Yang,Liangqi Cao,Heping Peng,Ping Xue 한국분자세포생물학회 2012 Molecules and cells Vol.33 No.6

MicroRNAs (miRNAs) are regulatory small non-coding RNAs that can regulate gene expression by binding to gene elements, such as the gene promotor 5UTR, mainly in the 3UTR of mRNA. One miRNA targets many mRNAs, which can be regulated by many miRNAs, leading to a complex metabolic network. In our study, we found that the expression level of miR-590-5p is higher in the human hepatocellular carcinoma cell line HepG2 than in the normal hepatocellular cell line L02. Downregulation of miR-590-5p inhibited proliferation and invasion of hepatocellular carcinoma cells (HCCs). We also showed that expression of TGF-beta RII, which has been regarded as a regulator of tumor proliferation, invasion, and migration in hepatocellular carcinoma, is regulated by miRNA-590-5p. In addition, miR-590-5p downregulated the expression of TGF- beta RII by targeting the 3UTR of mRNA. We also found that downregulation of miR-590-5p was associated with an elevation of TGF-beta RII and inhibition of proliferation and invasion in HepG2 cells. Furthermore, overex-pression of miR-590-5p was associated with upregulation of TGF-beta RII and could promote proliferation and invasion in L02 cells. In conclusion, we determined that TGF-beta RII is a novel target of miRNA-590-5p. Thus, the role of TGF-beta RII in regulating proliferation and invasion of human HCCs is controlled by miR-590-5p. In other words, miR-590-5p promotes proliferation and invasion in human HCCs by directly targeting TGF-beta RII.

The effect of viscosity ratio on drop pinch-off dynamics in two-fluid flow

Xiaofeng Jiang,Enle Xu,Xianliang Meng,Huai Z. Li 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.91 No.-

Drop pinch-off draws a lot of interest from scientists and engineers because of widely practical applications as well as the fascinating mechanism of finite-time singularities and self-similar behavior. This work experimentally investigates the effect of viscosity ratio on the local pinch-off mechanism of liquid drops in both air and viscous fluids, with a high-speed camera. The results show that for 56 ≤ λ ≤ 6.3 × 103 and 0.009 ≤ λ ≤ 0.061, drop pinch-off exhibits self-similar profile which is asymmetric and conical away from hmin. Drop pinch-off in air with low viscosity ratios shows either in inertial regime (I) or transition from inertial to viscous regime (I→V). But the kinetics undergoes the inertial regime (I) to inertial-viscous regime (IV) through an intermediate viscous regime (V) when the viscosity ratio becomes larger. Drop pinch-off in viscous fluids displays the transition from inertial to viscous regime (I→V). These results agree well with Eggers’s universal solution until it becomes unstable and the previous literature. The viscosity ratio indeed affects the drop pinch-off dynamics as well as interface deformation. Our experiments enrich the understanding of the interaction between the internal and external fluids on drop pinch-off behavior near the singularity point.

Predictors and Dynamic Nomogram to Determine the Individual Risk of Malignant Brain Edema After Endovascular Thrombectomy in Acute Ischemic Stroke

Qian-mei Jiang,Shuai Yu,Xiaofeng Dong,Huai-shun Wang,Jie Hou,Zhi-chao Huang,Zhi-liang Guo,Shou-jiang You,Guo-dong Xiao 대한신경과학회 2022 Journal of Clinical Neurology Vol.18 No.3

Background and Purpose This study aimed to construct an optimal dynamic nomogram for predicting malignant brain edema (MBE) in acute ischemic stroke (AIS) patients after endovascular thrombectomy (ET). Methods We enrolled AIS patients after ET from May 2017 to April 2021. MBE was defined as a midline shift of >5 mm at the septum pellucidum or pineal gland based on follow-up computed tomography within 5 days after ET. Multivariate logistic regression and LASSO (least absolute shrinkage and selection operator) regression were used to construct the nomogram. The area under the receiver operating characteristic curve (AUC) and decisioncurve analysis were used to compare our nomogram with two previous risk models for predicting brain edema after ET. Results MBE developed in 72 (21.9%) of the 329 eligible patients. Our dynamic web-based nomogram (https://successful.shinyapps.io/DynNomapp/) consisted of five parameters: basal cistern effacement, postoperative National Institutes of Health Stroke Scale (NIHSS) score, brain atrophy, hypoattenuation area, and stroke etiology. The nomogram showed good discrimination ability, with a C-index (Harrell’s concordance index) of 0.925 (95% confidence interval=0.890–0.961), and good calibration (Hosmer-Lemeshow test, p=0.386). All variables had variance inflation factors of <1.5 and tolerances of >0.7, suggesting no significant collinearity among them. The AUC of our nomogram (0.925) was superior to those of Xiang-liang Chen and colleagues (0.843) and Ming-yang Du and colleagues (0.728). Conclusions Our web-based dynamic nomogram reliably predicted the risk of MBE in AIS patients after ET, and hence is worthy of further evaluation.

Removal of Chromium (Ⅵ) by Escherichia coli Cells Expressing Cytoplasmic or Surface-Displayed ChrB: a Comparative Study

( Xiaofeng Zhou ),( Jianghui Li ),( Weilong Wang ),( Fan Yang ),( Bingqian Fan ),( Chenlu Zhang ),( Xiaojun Ren ),( Feng Liang ),( Rong Cheng ),( Fengying Jiang ),( Huaibin Zhou ),( Juanjuan Yang ),( 한국미생물 · 생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.7

Various genetically engineered microorganisms have been developed for the removal of heavy metal contaminants. Metal biosorption by whole-cell biosorbents can be enhanced by overproduction of metal-binding proteins/peptides in the cytoplasm or on the cell surface. However, few studies have compared the biosorption capacity of whole cells expressing intracellular or surface-displayed metal-adsorbing proteins. In this study, several constructs were prepared for expressing intracellular and surface-displayed Ochrobactrum tritici 5bvl1 ChrB in Escherichia coli BL21(DE3) cells. E. coli cells expressing surface-displayed ChrB removed more Cr(VI) from aqueous solutions than cells with cytoplasmic ChrB under the same conditions. However, intracellular ChrB was less susceptible to variation in extracellular conditions (pH and ionic strength), and more effectively removed Cr(VI) from industrial wastewater than the surface-displayed ChrB at low pH (<3). An adsorptiondesorption experiment demonstrated that compared with intracellular accumulation, cell-surface adsorption is reversible, which allows easy desorption of the adsorbed metal ions and regeneration of the bioadsorbent. In addition, an intrinsic ChrB protein fluorescence assay suggested that pH and salinity may influence the Cr(VI) adsorption capacity of ChrB-expressing E. coli cells by modulating the ChrB protein conformation. Although the characteristics of ChrB may not be universal for all metal-binding proteins, our study provides new insights into different engineering strategies for whole-cell biosorbents for removing heavy metals from industrial effluents.

Enhanced room-temperature HCHO decomposition activity of highly-dispersed Pt/Al2O3 hierarchical microspheres with exposed {110} facets

Xiaofeng Zhu,Jiaguo Yu,Chuanjia Jiang,Bei Cheng 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.45 No.-

Formaldehyde (HCHO) catalytic decomposition at room temperature is an important method for HCHOremoval in indoor environment. Herein,flower-like g-Al2O3 microspheres with high specific surface area,special textural structure, and abundant surface defects were prepared through a one-pot hydrothermalmethod by using aluminum foil as Al source. And it was used as oxide support to prepare highly-dispersed Pt catalyst (Pt/Al2O3). Compared with Pt supported on commercial g-Al2O3, the as-prepared Pt/Al2O3 showed enhanced catalytic activity for HCHO decomposition at room temperature. This workprovides new insights into designing and fabricating highly-dispersed Pt catalysts for efficient indoor airpurification.

MiR-126-3p inhibits apoptosis and promotes proliferation by targeting phosphatidylinositol 3-kinase regulatory subunit 2 in porcine ovarian granulosa cells

Zhou, Xiaofeng,He, Yingting,Jiang, Yao,He, Bo,Deng, Xi,Zhang, Zhe,Yuan, Xiaolong,Li, Jiaqi Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.6

Objective: Numerous studies have indicated that the apoptosis and proliferation of granulosa cells (GCs) are closely related to the normal growth and development of follicles and ovaries. Previous evidence has suggested that miR-126-3p might get involved in the apoptosis and proliferation of GCs, and phosphatidylinositol 3-kinase regulatory subunit 2 (PIK3R2) gene has been predicted as one target of miR-126-3p. However, the molecular regulation of miR-126-3p on PIK3R2 and the effects of PIK3R2 on porcine GCs apoptosis and proliferation remain virtually unexplored. Methods: In this study, using porcine GCs as a cellular model, luciferase report assay, mutation and deletion were applied to verify the targeting relationship between miR-126-3p and PIK3R2. Annexin-V/PI staining and 5-ethynyl-2'-deoxyuridine assay were applied to explore the effect of PIK3R2 on GCs apoptosis and proliferation, respectively. Real-time quantitative polymerase chain reaction and Western Blot were applied to explore the regulation of miR-126-3p on PIK3R2 expression. Results: We found that miR-126-3p targeted at PIK3R2 and inhibited its mRNA and protein expression. Knockdown of PIK3R2 significantly inhibited the apoptosis and promoted the proliferation of porcine GCs, and significantly down-regulated the mRNA expression of several key genes of PI3K pathway such as insulin-like growth factor 1 receptor (IGF1R), insulin receptor (INSR), pyruvate dehydrogenase kinase 1 (PDK1), and serine/threonine kinase 1 (AKT1). Conclusion: MiR-126-3p might target and inhibit the mRNA and protein expressions of PIK3R2, thereby inhibiting GC apoptosis and promoting GC proliferation by down-regulating several key genes of the PI3K pathway, IGF1R, INSR, PDK1, and AKT1. These findings would provide great insight into further exploring the molecular regulation of miR-126-3p and PIK3R2 on the functions of GCs during the folliculogenesis in female mammals.

Optimizing conical nozzle of venturi ejector in ejector loop reactor using computational fluid dynamics

Enle Xu,Xiaofeng Jiang,Long Ding 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.11

The structure of a conical nozzle is critical to the gas induction of a venturi ejector. In this work, the effect of nozzle structure on the gas induction was investigated by means of multiphase CFD and validating experiments. Under different structures, the maximal gas induction was obtained through analyzing the nozzle outlet velocity (NOV), nozzle inlet velocity (NIV), as well as nozzle shrinking angle (NSA). The simulated inlet pressure is positively proportional to inlet flow rate, which is in good agreement with experimental results. The simulated results reveal that the inlet pressure and gas induction increase with the increasing NOV. Considering the operational characteristics of centrifugal pump, the recommended NOV is about 21.8m/s. NIV and NSA show little impact on gas induction and inlet pressure. Based on the pipeline energy consumption, the recommended NIV is the same as the outlet velocity of centrifugal pump. The recommended NSA is about 20o to obtain the maximal gas induction.

Residual Stress Distribution in Hard-Facing of Pressure Relief Valve Seat

Ai, Li,Yu, Xinhai,Jiang, Wenchun,Woo, Wanchuck,Ze, Xiaofeng,Tu, Shan-Tung AMERICAN SOCIETY MECHANICAL ENGINEERS 2014 Journal of Pressure Vessel Technology Vol.136 No.6

<P>In this study, for the hard-facing of spring-loaded pressure relief valve seats, the residual stress distributions after the tungsten inert gas welding, (TIG) postwelded heat treatment and subsequent surface turning were investigated. The heat input parameters of welding were calibrated using an infrared imaging and thermocouples. The residual stress distributions were computed using three-dimensional finite element model. The neutron diffraction approach was employed to verify the finite element calculation. It is found that, the surface temperature during hard-facing welding shows a double ellipsoidal shape with the highest value of around 1570 degrees C. The high residual stress zones are located exactly under the welded joint except a slight deviation in the hoop direction. The magnitudes of tensile residual stresses in the three directions increase with their corresponding locations from the root of the joint into the base metal. The residual stresses in all of the three directions decrease significantly after the heat treatment. After surface turning, the residual stresses are tensile except for those close to the inner surface that are compressive in axial and radial directions.</P>

Synthesis and properties of amino acid functionalized water-soluble perylene diimides

Yongshan Ma,Xuemei Li,Xiaofeng Wei,Tianyi Jiang,Junsen Wu,Huixue Ren 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.7

We prepared amino acid functionalized water-soluble perylene diimides: N,N'-bi(L-glutamic acid)-perylene- 3,4;9,10-dicarboxylic diimide (1), N,N'-bi(L-phenylalanine acid)-perylene-3,4;9,10-dicarboxylic diimide (2), N,N'-bi(Lglutamic amine)-perylene-3,4;9,10-dicarboxylic diimide (3) and N,N'-bi(L-phenylalanine amine)-perylene-3,4;9,10- dicarboxylic diimide (4). The structures of 3 and 4 were confirmed by 1H NMR, FT-IR and MS. The maximal absorption bands of compound 1 and 2 in concentrated sulfuric acid were red-shifted for about 48 and 74 nm, respectively, compared with that of Perylene-3,4,9,10-tetracarboxylic acid dianhydride (PTCDA). Nearly no fluorescence was observed for compounds 1 and 2 in water, while compounds 3 and 4 were significantly water-soluble and had very high fluorescent quantum. The mechanism of the optical properties change was discussed, and the π-π stacking caused by H+ led to the changes of fluorescence spectrum and absorption spectrum. The calculated molecular orbital energies and the frontier molecular orbital maps of compounds 1-2 based on density function theory (DFT) calculations were reported. Owing to the high water-soluble, the perylene derivatives 3 and 4 were successfully applied as high-performance fluorochromes for living hela cells imaging.

Multi-Task FaceBoxes: A Lightweight Face Detector Based on Channel Attention and Context Information

( Shuaihui Qi ),( Jungang Yang ),( Xiaofeng Song ),( Chen Jiang ) 한국인터넷정보학회 2020 KSII Transactions on Internet and Information Syst Vol.14 No.10

In recent years, convolutional neural network (CNN) has become the primary method for face detection. But its shortcomings are obvious, such as expensive calculation, heavy model, etc. This makes CNN difficult to use on the mobile devices which have limited computing and storage capabilities. Therefore, the design of lightweight CNN for face detection is becoming more and more important with the popularity of smartphones and mobile Internet. Based on the CPU real-time face detector FaceBoxes, we propose a multi-task lightweight face detector, which has low computing cost and higher detection precision. First, to improve the detection capability, the squeeze and excitation modules are used to extract attention between channels. Then, the textual and semantic information are extracted by shallow networks and deep networks respectively to get rich features. Finally, the landmark detection module is used to improve the detection performance for small faces and provide landmark data for face alignment. Experiments on AFW, FDDB, PASCAL, and WIDER FACE datasets show that our algorithm has achieved significant improvement in the mean average precision. Especially, on the WIDER FACE hard validation set, our algorithm outperforms the mean average precision of FaceBoxes by 7.2%. For VGA-resolution images, the running speed of our algorithm can reach 23FPS on a CPU device.