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      • Dynamics System Analysis and Intelligent Identification of Aquaculture Water Quality Data

        Xiaofei Huang,Wei Wu 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.7

        Data analysis on environmental factors is crucial to aquaculture. Several significant parameters (temperature, pH and dissolved oxygen) and factors related to it are discussed in this paper. Data preparation including fixing some missing data and inaccurate records by non-linear function approaching in the first sampling process. Then the utilization of deterministic tracking theory is adopted for dynamic system analysis. Furthermore, time series analysis from different water layers based on this theory suits the real environment well, Radial Basis Function Neural Networks is well applied in tracking the parameters trend both globally and locally. The results provide effective references for systemic data analysis and control engineering.

      • SCIESCOPUS

        Displacement aging component-based stability analysis for the concrete dam

        Huang, Xiaofei,Zheng, Dongjian,Yang, Meng,Gu, Hao,Su, Huaizhi,Cui, Xinbo,Cao, Wenhan Techno-Press 2018 Geomechanics & engineering Vol.14 No.3

        The displacement monitoring data series reconstruction method was developed under equal water level effects based on displacement monitoring data of concrete dams. A dam displacement variation equation was set up under the action of temperature and aging factors by optimized analysis techniques and then the dam displacement hydraulic pressure components can be separated. Through the dynamic adjustment of temperature and aging effect factors, the aging component isolation method of dam displacement was developed. Utilizing the isolated dam displacement aging components, the dam stability model was established. Then, the dam stability criterion was put forward based on convergence and divergence of dam displacement aging components and catastrophe theory. The validity of the proposed method was finally verified combined with the case study.

      • KCI등재

        Comparison of Biological Characteristics of Human Umbilical Cord Wharton’s Jelly-Derived Mesenchymal Stem Cells from Extremely Preterm and Term Infants

        Huang Peng,Qin Xiaofei,Fan Chuiqin,Wang Manna,Chen Fuyi,Liao Maochuan,Zhong Huifeng,Wang Hongwu,Ma Lian 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.5

        BACKGROUND: Despite the progress in perinatal-neonatal medicine, complications of extremely preterm infants continue to constitute the major adverse outcomes in neonatal intensive care unit. Human umbilical cord Wharton’s Jellyderived mesenchymal stem cells (HUMSCs) may offer new hope for the treatment of intractable neonatal disorders. This study will explore the functional differences of HUMSCs between extremely preterm and term infants. METHODS: UMSCs from 5 extremely preterm infants(weeks of gestation: 22?5 w,24?4 w,25?3 w,26 w,28 w) and 2 term infants(39 w,39?2 w) were isolated, and mesenchymal markers, pluripotent genes, proliferation rate were analyzed. HUVECs were injured by treated with LPS and repaired by co-cultured with HUMSCs of different gestational ages. RESULTS: All HUMSCs showed fibroblast-like adherence to plastic and positively expressed surface marker of CD105,CD73 and CD90, but did not expressed CD45,CD34,CD14,CD79a and HLA-DR; HUMSCs in extremely preterm exhibited significant increase in proliferation as evidenced by CCK8, pluripotency markers OCT-4 tested by RT-PCR also showed increase. Above all, in LPS induced co-cultured inflame systerm, HUMSCs in extremely preterm were more capable to promote wound healing and tube formation in HUVEC cultures, they promoted TGFb1 expression and inhibited IL6 expression. CONCLUSIONS: Our results suggest that HUMSCs from extremely preterm infants may be more suitable as candidates in cell therapy for the preterm infants.

      • SERVICE RECOVER TIMING AND COMPENSATION STRATEGIES UNDER DIFFERENT INTENSITIES OF NEGATIVE EMOTIONS

        Xiaofei Tang,En-Chung Chang,Xing Huang,Meng Zhang,Beixi Wen 글로벌지식마케팅경영학회 2018 Global Marketing Conference Vol.2018 No.07

        The current study develops a pair model considering the intensity of negative emotions and strategic combinations of recovery means and timing under hotel service failure scenarios. The purpose of this paper is to evaluate the performances of these different combinations through customer satisfaction, repurchase intention, and fitting curves between the two under Chinese hotel service scenarios. Previous studies have found that service failures cause economic losses as well as negative emotional responses from customers (Gr?nroos, 1984; McColl Kennedy and Sparks, 2003). However, customers might display higher satisfaction and purchase intentions when proper service recovery actions are taken instead of when no service failures occur (Smith et al., 1999; McCollough et al., 2000). Concerning the means of service recovery during a service failure, customers in a negative emotional state require specific recovery solutions from the service provider; concerning the timing of service recovery, immediate recovery appears to be an effective way to address customer complaints (Kelley et al., 1993). While, previous research has partially shown that for extremely angry customers, the performance of “as soon as possible” recovery is unsatisfying and may even cause the service recovery to fail (Mattila and Ro, 2008). A 2 (recovery timing: immediate/delayed) × 2 (recovery means: psychological/economic) × 3 (type of service failure: failure in a delivery system/failure in responding to customer needs/improper employee behavior) between-subject experimental design was employed with 456 part-time MBA students in China to examine the relationship between customer satisfaction and repurchase intention under different levels of negative emotion after experienced a service failure. The results suggest that immediate and economic recovery effectively raises the service recovery evaluations from customers with low-intensity negative emotions, whereas delayed and psychological recovery helps customers with high-intensity negative emotions to give higher evaluations. This study sheds light on the role that negative emotions play in the process of service recovery and provides implications for service industry managers, that is the decisions to use economic recovery or psychological recovery are necessitate consider the intensity characteristic of negative emotions, for customers in low-intensity negative emotional states, the immediate and economic recovery strategy is effective, whereas for customers in high-intensity negative emotional states, the delayed and psychological recovery strategy may lead to better outcomes. According to these findings, it is important for managers to train front-line staff to adopt proper recovery strategies to manage service failure when facing consumers with different intensity of negative emotion.

      • KCI등재

        Characterization of Interphase Microbial Community in Luzhou-Flavored Liquor Manufacturing Pits of Various Ages by Polyphasic Detection Methods

        ( Hui Li ),( Jun Huang ),( Xinping Liu ),( Rongqing Zhou ),( Xiaofei Ding ),( Qianyin Xiang ),( Liqiang Zhang ),( Chongde Wu ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.1

        It is vital to understand the changing characteristics of interphase microbial communities and interspecies synergism during the fermentation of Chinese liquors. In this study, microbial communities in the three indispensable phases (pit mud, zaopei, and huangshui) of Luzhou-flavored liquor manufacturing pits and their shifts during cellars use were first investigated by polyphasic culture-independent approaches. The archaeal and eubacterial communities in the three phases were quantitatively assessed by combined phospholipid ether lipids/ phospholipid fatty acid analysis and fluorescence in situ hybridization. In addition, qualitative information regarding the microbial community was analyzed by PCR-denaturing gradient gel electrophoresis. Results suggested that the interphase microbial community profiles were quite different, and the proportions of specific microbial groups evolved gradually. Anaerobic bacteria and gram-positive bacteria were dominant and their numbers were higher in pit mud (10<sup>9</sup> cells/g) than in huangshui (10<sup>7</sup> cells/ml) and zaopei (10<sup>7</sup>cells/g). Hydrogenotrophic methanogenic archaea were the dominant archaea, and their proportions were virtually unchanged in pit mud (around 65%), whereas they first increased and then decreased in zaopei (59%-82%-47%) and increased with pit age in huangshui (82%-92%). Interactions between microbial communities, especially between eubacteria and methanogens, played a key role in the formation of favorable niches for liquor fermentation. Furthermore, daqu (an essential saccharifying and fermentative agent) and metabolic regulation parameters greatly affected the microbial community.

      • KCI등재

        Synthesis and Characteristic of Novel Soluble Triazoleimide Oligomers with Terminated Arylacetylene

        Xiao’an Zhou,Lei Du,Liqiang Wan,Xiaofei Wang,Yanpeng E,Farong Huang 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.9

        Novel soluble triazoleimide oligomers terminated with arylacetylene terminated were synthesized by the Cu(I)-catalysed 1,3-dipolar cycloaddition polymerization of diazides and imide-containing dialkyne. Several molecular weight triazoleimide oligomers were prepared from diazide and dialkyne monomers with different stoichiometric combinations. The curing behaviors of the oligomers were tested by differential scanning calorimetry (DSC). The thermal properties of the cured products were evaluated by DSC and thermogravimetric analysis (TGA). These cured oligomers showed the glass transition temperature of about 225 - 235 oC and the decomposition temperature (at 5% weight loss) of about 385 - 393 oC in nitrogen.

      • KCI등재

        miRNA-103a-3p Promotes Human Gastric Cancer Cell Proliferation by Targeting and Suppressing ATF7 in vitro

        Xiaoyi Hu,Jiyu Miao,Min Zhang,Xiaofei Wang,Zhenzhen Wang,Jia Han,Dongdong Tong,Chen Huang 한국분자세포생물학회 2018 Molecules and cells Vol.41 No.5

        Studies have revealed that miR-103a-3p contributes to tumor growth in several human cancers, and high miR-103a-3p expression is associated with poor prognosis in advanced gastric cancer (GC) patients. Moreover, bioinformatics analysis has shown that miR-103a-3p is upregulated in The Cancer Genome Atlas (TCGA) stomach cancer cohort. These results suggest that miR-103a-3p may function as an oncogene in GC. The present study aimed to investigate the role of miR-103a-3p in human GC. miR-103a-3p expression levels were increased in 33 clinical GC specimens compared with adjacent nontumor stomach tissues. Gain- and loss-of-function studies were performed to identify the correlation between miR-103a-3p and tumorigenesis in human GC. Inhibiting miR-103a-3p suppressed GC cell proliferation and blocked the S-G2/M transition in MKN-45/SGC-7901 cells, whereas miR-103a-3p overexpression improved GC cell proliferation and promoted the S-G2/M transition in vitro. Bioin-formatics and dual-luciferase reporter assays confirmed that ATF7 is a direct target of miR-103a-3p. Analysis of the TCGA stomach cancer cohort further revealed that miR-103a-3p expression was inversely correlated with ATF7 expression. Notably, silencing ATF7 showed similar cellular and molecular effects as miR-103a-3p overexpression, namely, increased GC cell proliferation, improved CDK2 expression and decreased P27 expression. ATF7 overexpression eliminated the effects of miR-103a-3p expression. These findings indicate that miR-103a-3p promotes the proliferation of GC cell by targeting and suppressing ATF7 in vitro.

      • KCI등재

        miRNA-103a-3p Promotes Human Gastric Cancer Cell Proliferation by Targeting and Suppressing ATF7 in vitro

        Hu, Xiaoyi,Miao, Jiyu,Zhang, Min,Wang, Xiaofei,Wang, Zhenzhen,Han, Jia,Tong, Dongdong,Huang, Chen Korean Society for Molecular and Cellular Biology 2018 Molecules and cells Vol.41 No.5

        Studies have revealed that miR-103a-3p contributes to tumor growth in several human cancers, and high miR-103a-3p expression is associated with poor prognosis in advanced gastric cancer (GC) patients. Moreover, bioinformatics analysis has shown that miR-103a-3p is upregulated in The Cancer Genome Atlas (TCGA) stomach cancer cohort. These results suggest that miR-103a-3p may function as an oncogene in GC. The present study aimed to investigate the role of miR-103a-3p in human GC. miR-103a-3p expression levels were increased in 33 clinical GC specimens compared with adjacent nontumor stomach tissues. Gain- and loss-of-function studies were performed to identify the correlation between miR-103a-3p and tumorigenesis in human GC. Inhibiting miR-103a-3p suppressed GC cell proliferation and blocked the S-G2/M transition in MKN-45/SGC-7901 cells, whereas miR-103a-3p overexpression improved GC cell proliferation and promoted the S-G2/M transition in vitro. Bioinformatics and dual-luciferase reporter assays confirmed that ATF7 is a direct target of miR-103a-3p. Analysis of the TCGA stomach cancer cohort further revealed that miR-103a-3p expression was inversely correlated with ATF7 expression. Notably, silencing ATF7 showed similar cellular and molecular effects as miR-103a-3p overexpression, namely, increased GC cell proliferation, improved CDK2 expression and decreased P27 expression. ATF7 overexpression eliminated the effects of miR-103a-3p expression. These findings indicate that miR-103a-3p promotes the proliferation of GC cell by targeting and suppressing ATF7 in vitro.

      • KCI등재

        An analytical approach of behavior change for concrete dam by panel data model

        Hao Gu,Meng Yang,Chongshi Gu,Wenhan Cao,Xiaofei Huang,Huaizhi Su 국제구조공학회 2020 Steel and Composite Structures, An International J Vol.36 No.5

        The behavior variation of concrete dam is investigated, based on a new method for analyzing the data model of concrete dam in service process for the limitation of wavelet transform for solving concrete dam service process model. The study takes into account the time and position of behavior change during the process of concrete dam service. There is no dependence on the effect quantity for overcoming the shortcomings of the traditional identification method. The panel data model is firstly proposed for analyzing the behavior change of composite concrete dam. The change-point theory is used to identify whether the behavior of concrete dams changes during service. The phase space reconstruction technique is used to reconstruct the phase plane of the trend effect component. The time dimension method is used to solve the construction of multi-transformation model of composite panel data. An existing 76.3-m-high dam is used to investigate some key issues on the behavior change. Emphasis is placed on conversion time and location for three time periods consistent with the practical analysis report for evaluating the validity of the analysis method of the behavior variation of concrete dams presented in this paper.

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