Nanoparticle Realgar Powders Induce Apoptosis in U937 Cells through Caspase MAPK and Mitochondrial Pathways

Xiao-bo Wang,Hui-yuan Gao,Bai-ling Hou,Jian Huang,Rong-gang Xi,Li-jun Wu 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.5

Nanoparticle realgar powders (NRP) inhibited U937 cell growth in a time and dose-dependent manner. U937 cells treated with NRP showed typical characteristics of apoptosis including the morphological changes and DNA fragmentation. Caspase family inhibitor (z-VAD-fmk), caspase-8, -9 inhibitor (z-IETD-fmk, Ac-LEHD-CHO, respectively) and caspase-3 inhibitor (z- DEVD-fmk) partially prevented NRP -induced apoptosis. Moreover, the classical substrates of caspase-3, poly-ADP ribose polymerase (PARP) was degraded after U937 cells treatment with NRP. In addition, NRP increased the ratio of Bax/Bcl-2 protein expression. Although p38 inhibitor (SB203580) and ERK inhibitor (PD98059) failed to block cell death, JNK inhibitor (SP600125) had marked inhibitory effects on NRP -induced apoptosis. Furthermore, the phosphorylation of JNK was up-regulated, suggesting that JNK was responsible for NRP -induced apoptosis in U937 cells. These results suggested that the caspase, mitochondria and MAPK signal pathways were involved in NRP-induced U937 apoptosis.

Surface quality prediction model of nano-composite ceramics in ultrasonic vibration-assisted ELID mirror grinding

Bo Zhao,Fan Chen,Xiao-feng Jia,Chong-yang Zhao,Xiao-bo Wang 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.4

Ultrasonic vibration-assisted Electrolytic in-process dressing (ELID) grinding is a highly efficient and highly precise machining method. The surface quality prediction model in ultrasonic vibration-assisted ELID mirror grinding was studied. First, the interaction between grits and workpiece surface was analyzed according to kinematic mechanics, and the surface roughness model was developed. The variations in surface roughness under different parameters was subsequently calculated and analyzed by MATLAB. Results indicate that compared with the ordinary ELID grinding, ultrasonic vibration-assisted ELID grinding is superior, because it has more stable and better surface quality and has an improved range of ductile machining.

Lentivirus-mediated RNA interference targeting E2F-1 inhibits human gastric cancer MGC-803 cell growth $in$ $vivo$

Wang, Xiao-Tong,Xie, Yu-Bo,Xiao, Qiang Korean Society for Biochemistry and Molecular Bion 2011 Experimental and molecular medicine Vol.43 No.11

The E2F-1 transcription factor is post-translationally modified and stabilized in response to various forms of DNA damage to regulate the expression of cell-cycle and pro-apoptotic genes. The sustained overexpression of E2F-1 is a characteristic feature of gastric cancer. In this study, we investigated the role of short hairpin RNA (shRNA) targeting E2F-1 gene on human gastric cancer MGC-803 cell growth $in$ $vivo$, and preliminarily revealed the mechanism. Thus, we constructed recombinant pGCSIL-GFP-shRNA-E2F-1 lentiviral vector to knock down E2F-1 expression in human gastric cancer MGC-803 cells $in$ $vivo$, and studied the effect of E2F-1 shRNA on growth of MGC-803 tumor and evaluated its treatment efficacy. Our data demonstrated that in a mouse model of established gastric cancer, intratumor injection of lentiviral shRNA targeting E2F-1 definitely decreased the endogenous E2F-1 mRNA and protein expression in MGC-803 tumor, and inhibited tumor growth and promoted tumor cells apoptosis. Moreover, we found that E2F-1 shRNA increased the expression of phosphatase and tensin homolog (PTEN), activated caspase-3 and caspase-9, and suppressed nuclear factor (NF)-${\kappa}B$ expression in tumor tissue as determined by reverse transcription (RT)-PCR and western blotting. In summary, shRNA targeting of E2F-1 can effectively inhibits human gastric cancer MGC-803 cell growth $in$ $vivo$ and may be a potential therapeutic strategy for gastric cancer.

Lentivirus-mediated RNA interference targeting E2F-1 inhibits human gastric cancer MGC-803 cell growth in vivo

Xiao-Tong Wang,Qiang Xiao,Yu-Bo Xie 생화학분자생물학회 2011 Experimental and molecular medicine Vol.43 No.11

The E2F-1 transcription factor is post-translationally modified and stabilized in response to various forms of DNA damage to regulate the expression of cell-cycle and pro-apoptotic genes. The sustained overexpression of E2F-1 is a characteristic feature of gastric cancer. In this study, we investigated the role of short hairpin RNA (shRNA) targeting E2F-1 gene on human gastric cancer MGC-803 cell growth in vivo, and preliminarily revealed the mechanism. Thus, we constructed recombinant pGCSIL-GFP-shRNA-E2F-1 lentiviral vector to knock down E2F-1 expression in human gastric cancer MGC-803 cells in vivo, and studied the effect of E2F-1 shRNA on growth of MGC-803 tumor and evaluated its treatment efficacy. Our data demonstrated that in a mouse model of established gastric cancer, intratumor injection of lentiviral shRNA targeting E2F-1 definitely decreased the endogenous E2F-1mRNA and protein expression in MGC-803 tumor, and inhibited tumor growth and promoted tumor cells apoptosis. Moreover, we found that E2F-1 shRNA increased the expression of phosphatase and tensin homolog (PTEN), activated caspase-3 and caspase-9,and suppressed nuclear factor (NF)-κB expression in tumor tissue as determined by reverse transcription (RT)-PCR and western blotting. In summary, shRNA targeting of E2F-1 can effectively inhibits human gastric cancer MGC-803 cell growth in vivo and may be a potential therapeutic strategy for gastric cancer.

Acoustic system design and experimental research on the ultrasonic forming grinding gear

Xiao-bo Wang,Mingqiang Wu,Jiangwei Jin,Bo Zhao 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.8

In order to obtain high-performance gear elements with integrated shape, using non-resonant theory, the gear grinding experiment and ultrasound-assisted grinding acoustic system were established. Firstly, a theoretical model founded on the non-resonant proposed methodology was constructed. The frequency equation and displacement characteristics of the vibration system were established based on the specified boundary conditions, and the geometric size of the ultrasonic amplitude with a design frequency of 20 kHz was established. The acoustic system was simulated employing finite element software, and the analysis revealed that its frequency range was 19648 Hz, which was consistent with the design outcome. Meanwhile, the amplitude of the end surface of the tool gear reaches 5.59 μm, and the vibration was stable. Secondly, according to the solution of geometric parameters, the acoustic performance test was carried out. The error between resonant frequency (20387 Hz) and design frequency was 1.93 %, and the amplitude was 4.5 μm, which met the requirements of ultrasonic assisted grinding gear. Finally, the grinding test was carried out. The study results show that compared to conventional gear grinding, ultrasonic supported grinding lessens the grinding force by 11.5 % to 35.4 % and, at most, lowers the grinding temperature by 22.5 %.

Nanoparticle Realgar Powders Induce Apoptosis in U937 Cells through Caspase MAPK and Mitochondrial Pathways

Wang, Xiao-bo,Gao, Hui-Yuan,Hou, Bai-ling,Huangi, Jian,Xi, Rong-gang,Wu, Li-Jun 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.5

School of Traditional Chinese Medicines, Shenyang Pharmaceutical University , Department of Pharmacy, Department of traditional Chinese MeNanoparticle realqar powders (NRP) inhibited U937 cell growth in a time and dose-dependent manner. U937 cells treated with NRP showed typical characteristics of apoptosis including the morphological changes and DNA fragmentation. Caspase family inhibitor (z-VAD-fmk), caspase-8, -9 inhibitor (z-IETD-fmk, Ac-LEHD-CHO, respectively) and caspase-3 inhibiter (z-DEVD-fmk) partially prevented NRP -induced apoptosis. Moreover, the classical substrates of caspase-3 poly-ADP ribose polymerase (PARP) was degraded after U937 cells treatment with NRP. In audition, NRP Increased the ratio of Bax/Bcl-2 protein expression. Although p38 inhibitor (SB203580) and ERK inhibitor (PD98059) failed to block cell death, JNK inhibitor(SP600125) had marked inhibitory effects on NRP -induced apoptosis. Furthermore, the phosphorylation of JNK was up-regulated, suggesting that JNK was responsible for NRP -induced apoptosis in U937 cells. These results suggested that the caspase, mitochondria and MAPK signal pathways were involved in NRP-induced U937 apoptosis.

Machine learning-based prediction and performance study of transparent soil properties

Bo Wang,Hengjun Hou,Zhengwei Zhu,Wang Xiao 국제구조공학회 2021 Smart Structures and Systems, An International Jou Vol.28 No.2

An indispensable process of geotechnical modeling with transparent soils involves analyzing images and soil property simulations. This study proposes an objective framework for quantitative analysis of the influential mechanism of three key factors, namely, different aggregate proportions (DAP), solvent ratio (SR), and solute solution ratio (SSR) on transparent soils’ transparency and shear strength. 125 groups of transparent soil samples considering these three factors were prepared to investigate their impact on transparency and shear strength through Elastic Net regression. Spearman correlation analysis was performed for transparency and shear strength. Furthermore, by comparing the performance of XGBoost, GBDT, Random Forest, and SVR after hyperparameter tuning in predicting transparency and shear strength, XGBoost proved to be the optimal machine learning model with the lowest MSE of 0.0048 and 0.0306 and was innovatively adopted to analyze how various factors affect the transparency and shear strength, thus enhancing the interpretability of machine learning. A ranking system, according to the importance scores of XGBoost, shows that SSR was the most important factor affecting both shear strength and transparency of transparent soils, with importance scores being 0.45 and 0.57, respectively. Our study may shed light on the preparation and performance study of transparent soils.

Pathogenicity of Five Strains of Toxoplasma gondii from Different Animals to Chickens

Shuai Wang,Guang-Wei Zhao,Wang Wang,Zhen-Chao Zhang,Bo Shen,I. A. Hassan,Qing Xie,Ruo-Feng Yan,Xiao-Kai Song,Li-Xin Xu,Xiang-Rui Li 대한기생충학열대의학회 2015 The Korean Journal of Parasitology Vol.53 No.2

Convenient and Robust Route to Photoswitchable Hierarchical Liquid Crystal Polymer Stripes via Flow-Enabled Self-Assembly

Li, Xiao,Li, Bo,He, Ming,Wang, Wei,Wang, Tianjie,Wang, Aurelia,Yu, Jiwoo,Wang, Zhonglin,Hong, Suck Won,Byun, Myunghwan,Lin, Shaoliang,Yu, Haifeng,Lin, Zhiqun American Chemical Society 2018 ACS APPLIED MATERIALS & INTERFACES Vol.10 No.5

<P>Hierarchically arranged stripes of photoswitchable liquid crystal polymers (LCPs) containing azobenzene moieties were conveniently crafted via a flow-enabled self-assembly (FESA). Interestingly, by subjecting a drop of LCP solution to dry in a restricted geometry comprising two nearly parallel plates with a stationary upper plate and a movable lower plate that programmably traveled in a 'stop -and-move' manner during the FESA process, photoswitchable LCP stripes were yielded, displaying two modes of deposition, namely, periodic primary stripes of large dimensions and regularly spaced secondary stripes of small dimensions situated between adjacent, primary stripes (i.e., forming hierarchical LCP stripes). Notably, these hierarchical azobenzene moieties-containing stripes demonstrated sequential photoinduced reversible phase transition (i.e., photoswitching) due to the thickness difference between primary and secondary stripes. A UV light-induced expansion effect. was observed on the LCP stripes. Clearly, such rapid creation of hierarchical stripes by FESA represents a robust means of organizing polymers, nanoparticles, colloids, DNA, etc. into complex yet ordered patterns over a large area in a simple and controllable manner for potential use in surface relief grating, photoactuators, photoswitchable devices, antifake labels, etc.</P>

Multicentre Hospital-based Case-control Study of Diffuse Large B-cell Lymphoma in Shanghai, China

Fan, Rong,Zhang, Lu-Yao,Wang, Hong,Yang, Bo,Han, Tao,Zhao, Xiao-Li,Wang, Wei,Wang, Xiao-Qin,Lin, Guo-Wei Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

Background: Several potential risk factors have been identified for diffuse large B-cell lymphoma (DLBCL); however, epidemiological studies investigating the association between these risk factors and DLBCL have yielded inconsistent results. Objectives: To investigate potential medical, lifestyle, and environmental risk factors of DLBCL in Shanghai, China through a hospital-based case-control study. Method: One-hundred-and-forty-seven newly diagnosed DLBCL patients and 294 sex- and age-matched controls were recruited from 11 hospitals in Shanghai between 2003 and 2007. A standardized structured questionnaire was used to obtain patient data on demographics, medical history, family history, lifestyle, and environmental exposures. Conditional logistic regression models were used to estimate odds ratios (ORs), with 95% confidence intervals (CIs), for risk associated with each data category. Results: History of tuberculosis (TB) infection and "living on a farm" were positively associated with DLBCL (TB: OR=3.05, 95% CI: 1.19-7.80; farm: OR=1.82, 95% CI: 1.21-2.73). In contrast, taking traditional Chinese medicine was negatively associated with DLBCL (OR=0.36, 95% CI: 0.14-0.89). No significant correlation with DLBCL risk was found for any of the other potential risk factors (p>0.05), including but not limited to hair dyes, alcohol drinking, smoking, and home/workplace renovation within one year. Conclusions: Consistent with results from previous studies in other DLBCL case populations, traditional Chinese medicine appeared to have a direct or indirect protective effect against DLBCL. However, this study also identified a possible predisposition for DLBCL in TB sufferers and farmers.