Systemic Reactions to Dust Mite Subcutaneous Immunotherapy: A 3-Year Follow-up Study

Xiang Dong,Nan Huang,Wenjing Li,Lintao Hu,Xiaolong Wang,Yin Wang,Ning Xiang,Guanghui Liu,Rongfei Zhu 대한천식알레르기학회 2016 Allergy, Asthma & Immunology Research Vol.8 No.5

Purpose: The incidence of allergen specific immunotherapy-related systemic reactions (SRs) varies among different studies, and many factors are likely to contribute to SRs. This study aims to investigate the incidence, characteristics, and risk factors of SRs to standardize dust mite-specific subcutaneous immunotherapy (SCIT) in Central China. Methods: All patients receiving standardized dust mites (100-100,000 SQ-U/mL; Alutard SQ, Hørsholn, Denmark) immunotherapy were followed up. Recorded data included demographics, diagnosis, patient status, pulmonary function testing results before and after each injection, allergen dosage, and details of SRs. Results: From June 2011 to August 2014, a total of 208 patients received 4,369 injections; 27 (13.0%) patients experienced 48 (1.1%) systemic reactions. Most of the SRs were grade 2 reactions (n=30, 62.5%), followed by grade 1 (n=11, 22.9%), grade 3 (n=7, 14.6%), and no fatal reactions occurred. Forty-six SRs (95.8%) occurred within 30 minutes. Higher SR rates were associated with high concentration extracts (100,000 SQ-U/mL), injections with concomitant local reactions (LRs), children, asthma and high sensitivity (skin prick test 3+/4+ and/or sIgE≥17.5 kUA/L) (P<0.05). The estimated odds of SRs increased in children (OR=6.57; 95% CI: 1.88-22.97, P=0.003), asthmatic patients (OR=4.10; 95% CI: 1.72-9.80, P=0.002), and injections with LRs (OR=2.41; 95% CI: 1.33-4.36, P=0.004).Conclusions: The incidence of SRs to dust mite SCIT was low, and multiple factors were associated with the increased incidence of SRs. Children, asthmatics and patients with concomitant LR may be prone to develop SRs.

Polyphyllin I combined with doxorubicin shows chemosensitization effect in vivo and reduces immunotoxicity of doxorubicin

Zhu Xiang,Na Xin,Zeng Yueqin,Xu Yangantai,Chai Dongya,Yang Huanzhi,Miao Jingqian,Zhang Yuan,Yang Fenghua,Wang Yuehu,Zhou Yiping 대한독성 유전단백체 학회 2022 Molecular & cellular toxicology Vol.18 No.3

Background Polyphyllin I (PPI), a steroidal saponin, exhibits antitumor activity and chemosensitization effect for a broad spectrum of cancer cells, however, its toxicity and chemosensitization effect in vivo is still unknown. Objective We investigated PPI’s cytotoxic activity, toxicity and chemosensitization effect and in vitro and in vivo . Results The IC 50 values of PPI on MCF-7, H22, and S180 tumor cells were 4.37 μmol/L, 1.71 μmol/L, and 0.92 μmol/L, respectively. The LD 50 of PPI was found to be 47.9 mg/kg using ip. injection. PPI at concentrations of 0.3 mg/kg, 0.6 mg/ kg, 1.2 mg/kg, and 2.4 mg/kg (1/80 LD 50 –1/20 LD 50 ) were synergized with DOX of 0.5 mg/kg to inhibit the H22 and S180 tumor growth in vivo by inducing apoptosis without obvious immunotoxicity. PPI exhibited a remarkable hemolytic effect on rabbit erythrocytes (EC 50 = 4.3 μM), while it had no impact in mice. Conclusion Our study revealed that the PPI-sensitized chemotherapeutic effect, when used in safe doses, circumvents immunotoxic side effects of DOX in vivo; thus, helping future clinical research.

A New Cytotoxic Phenazine Derivative from a Deep Sea Bacterium Bacillus sp

Zhu, Weiming,Li, Dehai,Wang, Fengping,Xiao, Xiang,Zeng, Xiang,Gu, Qian-Qun 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.5

A novel phenazine derivative (1) together with six known compounds (2-7) were isolated by bioassay-guided fractionation from the culture broth of a bacterium, Bacillus sp., collected from a Pacific deep sea sediment sample (depth 5059 m). The structures of these compounds were determined using spectroscopic methods. Their cytotoxic effects on P388 and K562 cell lines were preliminarily examined using the sulforhodamine-B (SRB) assay.

Application of Laparospic Ultrasonography in Surgery of Small Renal Cell Carcinoma

Wang, Xiang-Zhu,Yu, Ze-Xing,Guo, Rui-Jun,Xing, Nian-Zeng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21

Purpose: : To assess the clinic value of application of laparospic ultrasonography (LU) in partial nephrectomy of small renal cell carcinoma. Materials and Methods: From 2007 to 2011, 28 small renal cell carcinoma patients in ou clinic underwent laparoscopic partial nephrectomy with LU. For comparison with preoperative conventional ultrasound and CT, we collected ultrasonic performance of the affected side kidney, renal tumor location, size, echo change, blood supply situation and the relationship with the surrounding tissue. Results: LU could more clearly show the tumor interior structure and blood supply, as well as the relationship with the surrounding tissue. It also can provided doctor assistance with real-time tumor resection, reducing operative complications. Conclusions: LU can clearly show tumor internal structure and blood supply, which is helpful for explicit diagnosis. Moreover, it supplies accurate information for surgeons and assists surgery. Therefore LU has an important guiding value in partial nephrectomy for small renal cell carcinoma.

Improvement of Fumigaclavine C Production in a Two-stage Culture of Aspergillus fumigatus with Molasses as a Cost-effective Ingredient

Yi-Xiang Zhu,WEIWEI HUAN,Ling-yun Yao,Wan-Guo Yu,Ruihua Jiao,Yan-Hua Lu,Renxiang Tan 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.6

Fumigaclavine C (FC), which is produced by Aspergillus fumigatus, is a conidiation-associated ergot alkaloid with significant medical benefits. However, its application is restricted by low yields from submerged cultures. In this study, the technical feasibility of using molasses as a cost-effective ingredient for FC production in a two-stage culture of A. fumigatus was evaluated. The results indicated that molasses supplementation significantly enhanced FC accumulation by promoting conidiation and up-regulating hydroxymethylglutaryl-CoA reductase activity. Via the optimization of the two-stage process in the presence of molasses, FC production in shake flasks reached 226.9 mg/L, which was approximately three times that in the original medium (75.9 mg/L). The use of molasses as a cost-effective ingredient for FC fermentation was also successfully reproduced in a lab-scale bioreactor system in which the maximum FC production reached 215.0 mg/L. The FC production obtained in this study is the highest ever reported. This increased efficiency will enable large-scale production of FC and extend the application of molasses as a low-cost substrate for producing other conidiation-related secondary metabolites.

Effect of Filter Parameters on a Supercontinuum-Based All-Optical Tunable Thresholder

Huatao Zhu,Rong Wang,Tao Pu,Tao Fang,Peng Xiang,Huihui Zhu 한국광학회 2016 Current Optics and Photonics Vol.20 No.4

In this paper, the effects of filter parameters on a supercontinuum-based all-optical thresholder areexperimentally investigated. By tuning the filter parameters, the power transfer function and powertransmission function are tailored. The experimental results show that a thresholder with short centerwavelength has a better power function, and the slope in the middle level of the thresholder increaseswith increasing bandwidth. Through tuning the filter parameters, the thresholder can achieve a steplikepower transfer function for optical thresholding, and a steplike power transmission function for opticalself-switching. This makes the supercontinuum-based thresholder more flexible, and allows customizationof performance to meet different demands in various applications.

불소화 폴리아크릴레이트-폴리우레탄 라텍스 혼성필름의 그레디언트 구조와 표면성질

Min Zhu,Kun Chen,Yu Fang Zhang,Xiang Rong Wang,Xiang Dong Zhou 한국고분자학회 2014 폴리머 Vol.38 No.3

In order to investigate the characteristics of the gradient fluorinated polyacrylate and polyurethane latex blendfilms, the fluorinated polyacrylate emulsion and the polyurethane emulsion were synthesized, and then the both emulsionswere blended at a series of ratios. The effects of content of the fluorinated polyacrylate on the gradient structure and surfaceproperty of the blended films were assessed by AFM, XPS, SEM-EDX and surface free energy measurements. Itappeared that, while the content of the fluorinated polyacrylate latex was up to 30%, the fluorinated polyacrylate particleswere selectively gathered on the film-air (F-A) and film-glass (F-G) interfaces at room temperature. When the contentof the fluorinated polyacrylate was under 30%, the gradient structure of fluorinated component was not evident. The furtherincreasing of fluorinated polyacrylate in the mixed system facilitated the formation and enlargement of gradient structure,but the adhesion of film decreased a little.

陆游传世作品分析研究

朱翔宇(zhu, xiang-yu) 한국서예학회 2021 서예학연구 Vol.39 No.-

남송의 서예는 중국서예사상 비교적 쇠락한 시기로 북송만큼 뛰어난 서가들이 많지 않았으나 네 명의 서가들은 각자의 풍격으로 한 시대를 이끌었다. 육유는 시사(詩詞)로 세상에 이름났으나 그의 서예는 사람들에게 홀시되었다. 그는 행초서에 능하였는데, 해서는 안진경을 기초로 하였으며 행서는 소식을 위주로 여러 서예가들을 겸하여 학습하였다. 초서 쓰기를 가장 즐겼으며, 최종적으로는 각각의 자체(字體)에서 독특한 자가 풍격을 전개하였다. 그의 학서는 ‘남정지역 종군 시기’, ‘엄주지역 부임시기’, ‘사직 후 노년기’ 세 시기로 구분된다. 본 논문은 육유의 전세작품(傳世作品)을 착안점으로 하여 육유의 학서과정과 취법(取法)을 분석하였다. 또한 서예적 각도에서 육유 서예의 예술적 특징을 파악해 객관적이며 정확하게 육유 서예의 역사적 평가를 도출하고자 하였다. 본 논문은 육유의 작품 영상자료에 출발해 서예 자체의 기본 요소를 품평 기준으로 삼아 육유의 서예와 학서사상을 분석하였다. 그 중에서도 육유 작품에 대한 분석이 본 연구의 핵심이다. Calligraphy in the Southern Song Dynasty is a period of decline in the history of Chinese calligraphy. It is not as brilliant as the northern Song dynasty, but the four schools in the southern Song Dynasty were leaders in their own styles. Lu You is famous for his poetry, but his calligraphy has long been neglected. He is good at cursive script, his regular script is based on Yan Zhenqing. Running book to Su Shi based, and adopt various. Most like cursive script, and finally in all aspects of the script has a unique personal style show. His calligraphy study is divided into three periods: he joined the army for the Southern Expedition, he was appointed to Yan Zhou, and he died in Shan Yin. This paper takes Lu You s works as a starting point to analyze Lu you s learning process and method of learning calligraphy. This paper tries to grasp the artistic features of Lu You s calligraphy from the Angle of calligraphy itself. Thus objectively and accurately to Lu you s calligraphy historical evaluation. Based on the image data of Lu you s works, this paper takes the basic elements of calligraphy itself as the evaluation standard to analyze Lu you s calligraphy and his thoughts on learning calligraphy. The analysis of Lu you s works is the focus of this paper. 南宋书法是中国书法史上较为衰落的一个时期,其并未有如北宋一般群星璀璨,但是南宋四家却以各自风格领袖一时。陆游以诗词名世,其书法长期被人们所忽视。他擅长行草书,楷书以颜真卿为基础。行书以苏轼为主,兼采诸家。最喜作草,最终在各体方面都有独特的个人风格展现。他的书学分为三个时期,分别为从军南征,南征到严州任上,和致仕终老。本文以陆游的传世作品为切入点,分析陆游的学书历程与取法。本文力求从书法本身的角度来把握陆游书法的艺术特征。从而客观而准确的对陆游的书法进行历史性的评定。 本文从陆游的传世作品的图像资料出发,以书法本身的基本要素作为品评标准,对陆游的书法以及学书思想进行分析。其中对陆游传世作品的分析是本文的重点。

초기 북관개시(北關開市)와 야춘인(也春人)문제

주상길 ( Xiang Ji Zhu ),유희도 ( Xi Tao Liu ) 한국민족학회 2009 민족학연구 Vol.8 No.-

조선 후기 만주지역과 조선 간에 진행한 무역을 사행무역과 변시무역으로 구별할 수 있는데 이런 무역은 조공체제 하의 성격을 지니었다. 현재 사행무역에 대한 연구는 상당한 성과를 거두었으나 변시무역 특히 북관개시 중의 초기 회령개시 참가자인 야춘인과 경원개시가 열리게 된 원인에 대한 연구가 충분하게 진행되지 않았다. 본고는 선행 연구에 기초하여 중국과 한국의 사료를 접목시켜 호상 보충하는 방법으로써 초기 회령개시 청측 참가자 야춘인의 상황과 경원개시가 열리게 된 원인을 밝히고 나아가 선행 연구의 부족한 점도 보완하려고 한다. 1639년 청은 피역을 피해 웅도로 도망간 여진 와이객의 일부인 경하창 무리를 두 차례 정벌하여 그해 말에 이들을 야춘지역에 이주시키었다. 청은 이들 야춘인의 생활과 농경을 위하여 1642년 회령개시에 이들을 참가시켜 필요한 물화를 교역해 가게 하였다. 그 후 야춘지역의 일부 와이객이 혼춘지역에 이주하자 주변의 산재한 여진 종족들이 모여들어 인구가 급증하였다. 그리하여 혼춘지역과 야춘지역 와이객들의 생활과 농경을 위하여 청은 거리상 가까이에 있는 경원에서 개시할 것을 조선에 요청하였다. 경원개시가 열리게 되자 이들은 회령개시에 참가하지 않고 가까운 거리에 있는 경원개시에 참가하였다. 1654년에는 鴉客素河에서 新戶人들이 야춘지역에 이주해 왔고, 야춘지역에 남아있던 야춘인들과 함께 경원개시에 참가하였다. 그 후에 이들도 혼춘에로 이주하면서 경원개시에 참가하였다. 이는 초기 회령개시와 경원개시를 열게 된 원인과 『通文館志』事大上開市條에 나오는 개시에 참가한 야춘인들에 대한 해석이다. 또한 이 연구는 차후 두만강 이북과 이동 지역사의 연구와 조선인들의 간도에로의 이주에도 밑거름이 되는 작업이라고 생각된다. 이런 조선 후기의 변시무역은 1882년에 중단되고 근대식 자유통상방식으로 전환되었다. In the Qing Dynasty, the trade between Northeast China and Korea can be divided into two parts: ambassador trade and the frontier mutual trade. At that moment, the trade between the Qing Dynasty and Korea has the nature of the tributary system. Now the study of ambassador trade have achieve fairly good results, but the study of the frontier mutual trade between the Qing Dynasty and Korea is not full and detailed, especially (the study)of the initial period of the opening in the Beiguan market and its participants-the people of Yechun. In this paper, with the historical materials of China and Korea, the author uses the methods of complementary and textual research to reveal the process of trade among the participants of the initial period of the opening in the Beiguan market and the reason for opening the market in Qingyuan. Thereby, we can find out the development of Hunchun and its trade embryonic with Korea in the late Ming and early Qing dynasty, and then lay the foundation for the study of the frontier mutual trade between the Northeast China and Korea in the Qing Dynasty.

Fucoidan inhibits LPS-induced acute lung injury in mice through regulating GSK-3β-Nrf2 signaling pathway

De-Zhang Zhu,Yan-Ting Wang,Yan-Li Zhuo,Kong-Juan Zhu,Xiang-Zhen Wang,Ai-Jie Liu 대한약학회 2020 Archives of Pharmacal Research Vol.43 No.6

The purpose of this study was to investigatethe protective eff ects of fucoidan on Lipopolysaccharide(LPS)-induced acute lung injury (ALI) in mice. The micewere divided into the control, LPS, and LPS + fucoidan(20, 40, or 80 mg/kg) groups. LPS was given by intrachealinstillation and fucoidan was given 1 h before LPS treatment. Myeloperoxidase (MPO) activity, malondialdehyde(MDA), superoxide dismutase (SOD), reactive oxygen species(ROS), glutathione (GSH) contents, and infl ammatorycytokine production were detected. The results showed thatLPS-induced TNF-α, IL-1β, and IL-6 production, lung wet/dry (W/D) ratio, ROS, MDA content, and MPO activity weresuppressed by fucoidan. The levels of SOD and GSH wereincreased by fucoidan. Meanwhile, LPS-induced nuclearfactor kappa-B (NF-κB) activation was dose-dependentlyattenuated by fucoidan. Furthermore, fucoidan increasedthe expression of nuclear factor erythroid-2 related factor2 (Nrf2), Glycogen synthase kinase3β (GSK-3β), and hemeoxygenase (HO-1). In vitro, the results demonstrated thatfucoidan or GSK-3β inhibitor signifi cantly inhibited LPSinducedTNF-α production in A549 cells. And the inhibitionof fucoidan on TNF-α production was blocked by Nrf2siRNA. This study showed fucoidan protected mice againstLPS-induced ALI through inhibiting inflammatory andoxidative responses via regulating GSK-3β-Nrf2 signalingpathway.