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ICR mouse의 피부와 간에서 자외선 B파에 의한 Heme Oxygenase-1의 유도
최욱희,김태환,안령미,Choi, Wook-Hee,Kim, Tae-Hwan,Ahn, Ryoung-Me 한국환경보건학회 2008 한국환경보건학회지 Vol.34 No.1
The induction of heme oxygenase-1(HO-1) by UV radiation provides a protective defence against oxidative stress, and has been well demonstrated in skin irradiated with UVA, but not UVB. In this study, we show that the induction of cutaneous HO-l can be attributed to UVB radiation. The expression of HO-1 mRNA was assessed in vivo by reverse transcription-polymerase chain reaction (RT-PCR) analysis, and HO-1 enzyme activity was measured in microsomal preparation from irradiated mice. The mRNA level of HO-1 increases in liver and skin from 1d to 3d after UVB $(3KJ/m^2)$ exposure. The results of gene expression were same pattern of HO-1 enzyme activity in skin, but not in liver. HO-1 mRNA in liver resulted in a progressive increase to 4d after UVB exposure, but HO-1 activity in liver increased to 2d. This finding indicates that UVB radiation is an important inducer of HO-1 and increases in HO activity may protect tissue directly or indirectly from oxidative stress.
천연물 도포가 UVB 파로 손상된 C57BL/6 mouse 피부의 색소침착과 염증생성에 미치는 영향
최욱희,안형수,최태윤,진소영,안령미,Choi, Wook-Hee,Ann, Hyoung-Soo,Choi, Tae-Youn,Jin, So-Young,Ahn, Ryoung-Me 한국환경보건학회 2006 한국환경보건학회지 Vol.32 No.5
Ultraviolet(UV) radiation causes a variety of biological effects on the skin, including inflammation, pigmentation, photoaging and cancer. Free radicals are involved in inflammatory skin reactions induced by UVB radiation. In this study, we investigated the effects of antioxidants(Tea, Korean red ginseng, Ginkgo biloba extract) on UVB-induced skin damage. Tea, KRG and EGb 761 were topically treated to dorsal skin of ICR mouse. The mice were also treated soon after IMED ($1.4KJ/m^{2}$) of UVB irradiation. Skin pigmentation of irradiated mouse was observed by a chromameter after 2 weeks. Topical application of Tea, KRG and EGb 761 for 2 weeks decreased skin pigmentation compared to DVB control group(p<.05). Tea, KRG and EGb 761 also reduced UVB-induced infiltration of inflammatory cells. These results showed that Tea, KRG and EGb 761 as a topical application may have preventive effect against UVB-induced skin damage.
ICR mouse에 있어 UVB조사로 유도된 접촉 과민반응에 대한 EGb 761의 억제 효과
최욱희,안형수,안령미,Choi, Wook-Hee,Ann, Hyoung-Soo,Ahn, Ryoung-Me 한국환경보건학회 2005 한국환경보건학회지 Vol.31 No.1
Exposure of skin to UVB radiation can cause the induction of inflammation and impairment of contact hypersensitivity(CHS) response. Several studies have shown that polyphenolic compounds isolated from EGb 761 afford protection against UVB. In this study, we demonstrated that topical application of EGb 761, before 1MED(1.4 KJ/$m^2$), 1.5MED (2.1 KJ/$m^2$), 2MED (2.8 KJ/$m^2$) of UVB exposure to ICR mice prevented UVB-induced inflammation and inhibition of the contact hypersensitivity response. The skin-fold swelling from 1MED, 1.5MED, 2MED of UVB exposure highly significantly increased after twice irradiation. Topical application of EGb 761(0.1%, 1%, 4%), 5 days prior to UVB exposure reduced skin thickness compared to non-treated mice. Exposure of shaved abdominal skin of mice to 1MED, 1.5MED and 2MED of UVB radiation resulted in suppression of contact sensitization through the skin to 56.23%, 65.12%, 74.02%, compared to normal unirradiated skin. Topical application of EGb 761(0.1%, 1%, 4%), 5 days prior to or 5 days after exposure to 1MED and 2MED of UVB resulted in protection against suppression of contact hypersensitivity in mouse dorsal skin. These protective effects were dependent on the dose of EGb 761 employed. The present study show that EGb 761 protect UVB-induced inflammation and immune suppression. Also, we suggest that EGb 761 can provide protection from photoimmunosuppression.
펫동반 관광제약이 협상, 행동의도 및 추가지불의도에 미치는 영향
최욱희(Wook-Hee Choi),이은경(Eun-Kyoung Lee) 한국자료분석학회 2020 Journal of the Korean Data Analysis Society Vol.22 No.1
The purpose of this study was to examine the relationships among tourism constraints with pets, negotiation, behavioral intention and willingness to pay more for pet owner s tourism activities. To verify the hypothesis, A survey was conducted for pet companion who have visited during “The pet & fam festival” held in Busan and ultimately 230 samples were analyzed. The collected data was used for the empirical analysis by utilizing the statistical package programs SPSS 20.0 and AMOS 20.0. The exploratory factor analysis, reliability analysis and confirmatory factor analysis were conducted to verify the validity and reliability of examining items, to verify the hypothesis, structural equation model was conducted. The results of structural equation modeling indicate that tourism constraints with taking pets to tourism activities had a positive effect partially on negotiation. Also negotiation was found to have a positive effect on behavioral intention and willingness to pay more. The result is when there is constraints on tourism activities of pet companion, they would participate in tourism activities through negotiation even though willingness to pay. The findings of study suggest that the greater pet companions tourist have constrain and the more they perceive them positively, the more they are likely to strengthen negotiation efforts which increase their behavioral intention. 본 연구는 펫 동반자의 관광활동 제약요인에 대해 확인하고, 각 제약요인들이 협상, 행동의도와 추가지불의도에 미치는 영향에 대해 검증하고자 하였다. 가설검증을 위해 국내에서 개최된 ‘펫 & 팸 페스티벌’ 기간 동안 방문한 반려인을 대상으로 설문조사를 실시하여 자료를 수집하였으며, 최종 230명의 표본을 사용하였다. 측정 항목의 타당성과 신뢰성을 검증하기 위해 SPSS 20.0과 Amos 20.0 프로그램을 사용하여 신뢰도 분석, 탐색적 및 확인적 요인분석을 실시하였고 가설검증을 위해 구조방정식모형 검증을 실시하였다. 연구결과, 첫째, 펫동반 관광제약은 협상에 부분적으로 정의 방향으로 유의한 영향을 미치는 것으로 나타났다. 둘째, 협상이 행동의도에 정의 방향으로 유의한 영향을 미치는 것으로 나타났다. 마지막으로 협상은 추가지불의도에도 정의 방향으로 유의한 영향을 미치는 것으로 나타났다. 이러한 결과는 펫 동반 관광자의 관광활동에 제약 발생 시 적절한 준비과정과 이를 극복하기 위한 노력을 강화하는 협상을 통해 이에 드는 비용과 노력이 더 들더라도 기꺼이 지불하고 참여하겠다는 반려인의 관광참여 행동의도와 추가지불의도를 확인할 수 있다. 본 연구는 향후 반려동물을 동반한 관광자의 니즈가 확대됨에 따라 예상되는 제약을 완화시키고 반려인과 비반려인이 모두 만족하는 관광활동의 긍정적 확장을 위한 방안으로 시사점을 제시하였다.