Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

Mycobacterium intracellulare에 의한 요추부 척추염 1예

장은영,김미영,김정욱,송은희,백관미,정용필,성흥섭,김미나,김양수,우준희,이상호 대한감염학회 2007 감염과 화학요법 Vol.39 No.3

비결핵성 미코박테리아에 의한 척추염에 대한 증례 보고가 국내에서는 아직 없었다. 저자들은 기저질환이 없는 환자에서 M. intracellulare에 의한 척추염을 rpoB 유전자에 대한 PCR-RFLP 방법을 통해 진단하였고 수술적 치료와 3제 병합 항균 요법으로 성공적인 임상 경과를 보인 1예를 경험하였기에 보고하는 바이다. We report a case of vertebral osteomyelitis due to Mycobacterium intracellulare in a 60-year-old man without predisposing conditions. He was successfully treated with surgical excision of the inflamed tissue and combined antimycobacterial therapy consisting of clarithromycin, ethambutol, and rifampin. In this case, polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP) based on the rpo B gene of cultured isolates allowed rapid identification of M. intracellulare. Although very rare, nontuberculous mycobacteria (NTM) should be suspected as a causative pathogen of vertebral osteomyelitis. To the best of our knowledge, this is the first case of vertebral osteomyelitis caused by NTM reported in the Korean literature.

시각적 피드백을 병행한 어깨복합운동프로그램이 어깨비대칭 청년들의 어깨 근력과 정렬에 미치는 영향

강순희, 강은지, 목미경, 박지연, 손혜림, 연우정 한국교통대학교 2016 한국교통대학교 논문집 Vol.51 No.-

The purpose of this study was to identify whether shoulder complex exercise program with visual feedback could improve the shoulder muscular strength and postural alignment in adolescents with shoulder asymmetry. Twenty eight subjects were recruited from the K university and divided into three groups: experimental 1, 2group(Each n=10) and control group(n=8). For 4 weeks, the experimental group 1 performed shoulder muscle complex exercise program and the experimental group 2 performed shoulder complex exercise program with visual feedback. The control group did not perform exercises. The shoulder muscle strength and difference between the height of left and right shoulders were assessed before, after training using the hand-held dynamometer and Global Posture System. Subordination variables, general characteristics analysis, χ² test and Kruskal Wallis H test were conducted to evaluate differences between groups and training before, after changes were checked Wilcoxon signed rank test with using the PASW 18.0 statistical program. The results of the study were as follows. 1) Both the experimental 1, 2group showed improvements in muscle strength of several muscles following training (p<.01). The control group showed significant reduction in the left posterior deltoid (p<.05), and did not show significant changes in examining other muscles (p>.05). And there were differences among three groups in shoulder muscle strength (p<.001). Muscle strength changes in several muscles were greater in the experimental 1, 2group compared with the control group (p<.001). Muscle strength changes in the several muscles were greater in the experimental group 2 compared with the experimental group 1 (p<.001) 2) Both the experimental 1, 2group showed significant decreases in the difference between the left and right shoulder heights following training (p<.01). The control group did not show change (p>.05). Changes in the difference between the left and right shoulder heights were greater in the experimental 1, 2group compared with the control group (p<.001).

Asimina triloba 씨앗으로부터 분리된 Annonaceous acetogenin 으로서의 Xylomaticin

우미희,김달환 대구효성카톨릭대학교 1997 연구논문집 Vol.56 No.2

Most Annonaceous acetogenins are potently bioactive and offer exciting potential as new antitumor, pesticidal and other bioactives. The major mode of action of the acetogenins is inhibition of electron transport in the mitochondria. Acetogenins are waxy substances consisting of C32 or C34 long chain fatty acids which have been combined with a propan-2-ol unit at C-2 to form a Y-lactone. Acetogenins have been isolated from an extract of the seeds of Asimina triloba by directing the fractionation with brine shrimp lethality. The structures were characterized on the basis of chemical and spectral data. Xylomaticin, which is known but is first isolated in this species, was obtained. In brine shrimp lethality test(BST), xylomaticin exhibited cytotoxicity.

Polarography 法에 依한 p-Nitrobenzaldoxime의 定量

禹美熙 대구효성가톨릭대학교 1984 연구논문집 Vol.28 No.2

The polarographic method is very sensitive, selective and less time consuming than other methods recommended for the determination of p-nitrobenzaldoxime. The object of this paper is to study the electroreducations of p-nitrobenzaldoxime formed by the reaction of p-nitrobenzaldehyde and hydroxylamine hydrochloride, and p-nitrobenzaldehyde single. Dropping mercury electrode (DME), Ag/AgCl electrode and Pt wire were served as working, reference and auxiliary electrode, respectively. The capillary characteristics of the DME at a mercury height of 70cm were m=0.90 mg. sec? and t=2.0sec. All experiments were performed at 25±0.2℃. Dissolved air was removed from the solution by bubbling oxygen-free nitrogen through the cell for 10 min. In order to establish the optimum conditions of p-nitrobenzaldoxime's polarogram. we investigated the effects of alcohol concentration, supporting electrolyte, pH, temperature and standing time. Polarogram of p-nitrobenzaldoxime recorded from 0.1M sulfuric acid exhibits a well-defined 4-electron reduction wave. The a.c. current is diffusion-controlled and increases linearly with the concentraion of p-nitrobenzaldozime. The half potential of p-nitrobenzaldoxime was -72 V vs. Hg pool. The linear calibration curve was obtained in the range of 40~160㎍/ℓ. Thus, the polarographic determination of p-nitrobenzaldoxime was more sensitive above 10-fold than p-nitrobenzaldehyde.

냉기 치료에 따른 둔근에서의 피부 및 근육내 온도 변화

정우성,김미정,박시복,이상건,김영호,양길태,장윤희 大韓再活醫學會 1998 Annals of Rehabilitation Medicine Vol.22 No.2

호텔업의 신사업 진출전략에 관한 연구

원융희,이미혜 용인대학교 산업경영연구소 2004 산업경영논총 Vol.11 No.-

The purpose of this study is to improve brand image and contribute to the growth of Hotels by suggesting appropriate business management strategies for the approach in the context of an managemental point of view in order to reflect the current trend of Hotel managements. In those days, Hotels are a deep competitive relation of others. Under the current situation, it is very important to activate and strengthen the competitiveness of Hotels. Therefore Hotels advance the new business outsides, specially is foodservice. At once, Hotels are inroading foodservie industry because maintances Sustainable Competitive advanges(SAC). The new business strategies of Hotels foodservices is suggested by three parts, which are cost efficiency, individualization, service quality. To achieve the purpose of this study, literature research and cases collects, and objective and scientific analysis are done.

초·중학생 어머니의 양육스트레스와 언어적 학대와의 관계

우희정,최정미 한국 가정과 교육 학회 2002 한국가정과교육학회지 Vol.14 No.3

The purpose of this study was to investigate the relationship between parenting stress and verbal abuse of elementary and middle school students' mothers. The subject were 659 adolescences and their mothers. Data was analyzed by t-test, ANOVA, correlation. In the study, generally mother's parenting stress level and mother's verbal abuse level tent to be below the mean scores. Parenting stress related to learning expectation is significant difference to adolescent's sex/grade, but verbal abuse is not significant difference to the sex/grade. And parenting stress related to temperament and learning expectation is significant difference to mother's education level. And as for correlating parenting stress to verbal abuse, the significance appeared in these factors.

Asimina triloba씨앗으로부터 세포 독성 물질인 Annonaceous acetogenin으로서의 asimin

우미희 대구효성가톨릭대학교 1998 연구논문집 Vol.57 No.2

Acetogenins are waxy substances consisting of C32 or C34 long chain fatty acids which have been combined with a propan-2-ol unit at C-2 to form a Y-lactone. Their diverse bioactivities as antitumour, immunosuppressive, pesticidal, anthelmintic and antimicrobial agents have attracted more and more interest worldwide. Acetogenin has been isolated from an extract of the seeds of Asimina triloba by directing the fractionation with brine shrimp lethality test. The structure was characterized on the basis of chemical and spectral data. Asimin, which is known but is first isolated in this species, was obtained. In brine shrimp lethality test (BST), asimin exhibited cytotoxicity.

효모시스템에서 Human Transglutaminase C(TGase 2)의 발현에 관한 연구 : C-말단부위의 결손효과 An Effect of a Domain from Carboxyl Terminal Deletion of the Enzyme

우상규,정선미,이상기,안병윤,김희철 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.3

Transglutaminase(TGase Ⅱ)는 펩타이드-결합 글루타민 잔기와 펩타이드-결합 라이신잔기 및 1차 아민류를 포함하는 다양한 화합물과의 교차결합에 의하여 단백질의 변형을 촉매하는 효소이다. 동물세포에는 구별가능한 5종류의 TGase가 존재한다[TGase Ⅰ(또는 K), Ⅱ(C), Ⅲ(E), Ⅳ(P)와 혈장단백 ⅩⅢa]. 그들은 아미노산 서열에서 N-말단에 약 70%의 상동성을 갖고 있으며, C-말단의 경우에는 거의 상동성이 부위(N-말단 2/3)와 비상동성 부위(C-말단 1/3 부위)의 역할을 알아보기 위해 전체효소 또는 C-말단이 결손된 형태의 효소를 Saccharmoyces cerevisiae에서 발현 하고자 하였다. Southern blot, 역전사 중합 연쇄 반응, Western blot등을 통해 전체효소 및 C-말단 결손형 효소가 형질전환된 효모에서 발현되는 것을 확인하였다. Succinylated casein에 [1,4-^14C]-putrescine이 통합되는 반응을 이용한 활성 측정에서 C-말단이 결손된 TGase Ⅱ는 같은 세포 농도에서 전체 TGase Ⅱ 효소와 비교하여 유사한 수준의 활성을 나타내었다. 이러한 결과들은 사람 TGase Ⅱ의 경우 N-말단의 높은 상동성부위가 효소의 역가결정에 중요하다는 것을 나타내 준다. 효모시스템에서 발현한 결과 효소의 역가가 나타났으나 높지 않았으며 발현된 양이 극히 낮아서 단백질의 정제나 특성연구를 위하여 발현벡터의 개량을 포함한 방법의 개선이 필요하다고 사료된다. 흥미롭게도 TGase Ⅱ의 발현이 효모의 성장을 저해하는 것으로 나타나 이러한 현상이 표피세포의 각질화에 수반된 세포의 죽음과 유관한지의 가능성에 대한 보다 자세한 연구가 필요하다. In an effort to understand the role of the conserved domain and of the heterologous one-third part of the carboxy terminal domain of transglutaminase C (TGase Ⅱ), attempts were made to express TGase Ⅱ cDNA of human origin in yeast Saccharmoyces cerevisiae as in a full-length form as well as in a form of C-terminal truncation. The 2μ-based expression plasmids which contained the TGase Ⅱ cDNA under the gal inducible promoter were introduced into yeast and the maintenance of the full-length and truncated form of the TGase Ⅱ gene plasmids were confirmed by Southern blot. The expression of e results indicate that the N-terminal homologous domain of human TGase Ⅱ retains a catalvtically active domain. The level of TGase Ⅱ expressed in yeast, however, was far lower than satisfactory and other expression system should be sought further characterization of the enzyme. The negative effect of TGase Ⅱ on the growth of yeast is interesting with respect to the physiological effect of TGase Ⅱ in the cornification f epidermal keratinocytes.