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Wontae Kim,Sungwoo Bae,Kwanho Park,Sang beom Lee,Youngcheol Choi,Youngho Koh 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.10
The black soldier fly (BSF), Hermetia illucens, is known as a beneficial insect and feeds on organic materials derived from animals and human, resulting in reduction of food waste and conversion of organic materials. Despite of many studies on the BSF, there have been no reports of cloned genes encoding serine proteases in the BSF. Thus, the primary objective of this study is to clone and to investigate expression pattern of genes encoding serine proteases released from the midgut of the BSF larvae in order to gain a better understanding of expression mechanism of serine proteases. We cloned two serine proteases from the BSF larva. Based on phylogenetic tree analysis, one was chymotrypsin, the other was trypsin. The open reading frame (ORF) of chymotrypsin was 804bp, which encoded a polypeptide of 267 amino acids. In case of trypsin, the ORF was 744bp, which encoded a polypeptide of 247 amino acids. To investigate expression pattern of two serine proteases, we conducted semi-quantitative RT-PCR at different tissues and different developmental stages. A chymotrypsin and trypsin transcripts were revealed strongly in mid gut. Especially, a chymotrypsin was detected largely at feeding stage more than molting stage, while trypsin was expressed similarly between feeding stage and molting stage
이원태(Wontae Lee),김영현(Younghyun Kim) 한국소음진동공학회 2015 한국소음진동공학회 학술대회논문집 Vol.2015 No.10
The steering system of forklifts should have enough rigidity in order to minimize deformation caused by the wheel operation and to block the vibrations from engine, hydraulic systems and the road surface. Especially, the steering system of the small-class forklift has disadvantage in vibration transmission path since it is difficult to obtain proper structural stiffness. In this paper design optimization technique of the steering system for small-class forklifts considering idle vibration reduction is presented. Two types of engines are mounted with small-class forklift; 2-ton class forklift with 3-cylinder and 3-ton class forklift with 4-cylinder diesel engine. Considering the excitation frequency of the engines, the vehicle body and steering system of the forklift were designed to minimize the idle vibration.
화학침전을 이용한 발효액의 젖산 회수 및 유기물 특성분석
이원태(Wontae Lee) 유기성자원학회 2018 유기물자원화 Vol.26 No.1
유기성 폐기물 소화공정의 발효액에서 젖산(lactic acid)을 회수하기 위해 화학침전법을 평가하였다. 젖산(lactic acid)의 회수율을 높이기 위하여 화학침전제 종류와 교반속도 및 침전시간 등 반응조건이 회수율 향상에 미치는 영향을 살펴보았다. 화학침전제의 종류에 관계없이 주입양이 증가할수록 젖산(lactic acid) 회수율 이 증가하는 경향을 보였으며, CaO가 Ca(OH)2와 CaCO3에 비하여 높은 회수율을 나타냈다. CaO를 사용한 반응조건 최적화 실험결과, 교반속도 180 rpm, 침전시간 24 h, ethanol 주입량 25%(v/v)에서 회수율이 48%로 가장 높게 나타났다. 본 연구에서는 발효액 내 젖산(lactic acid)의 농도만 고려하여 주입할 침전제의 양을 계산하였기 때문에 실제 적용을 위해서는 발효액의 유기산 종류 및 농도를 고려하여 침전제의 투입량을 결정해야할 것이다. 유기물의 정성적 분석(FEEM, SEC) 결과로 볼 때 침전공정은 유기산의 특성에 큰 영향을 미치지 않았다. Recovery of lactic acid from fermentation broth using chemical precipitation was investigated with various chemicals. Effects of chemical types, mixing speeds, settling duration, and solvent addition were evaluated to improve the recovery rates of lactic acid. Overall, recovery efficiencies increased as the dosage of chemicals increased. Recovery rate of lactic acid by CaO was higher than those of Ca(OH)2 and CaCO3. Recovery of lactic acid increased by 48% under the optimized reaction conditions which included a mixing speed at 180 rpm, a settling duration of 24 h, and addition of ethanol at 25%(v/v). Practical application needs to consider types and concentrations of other organic acids as well as lactic acid. Based upon the results of fluorescence excitation emission matrix (FEEM), size exclusion chromatography (SEC), characteristics of recovered lactic acid were same as that in the fermentation broth.
소형 지게차의 Idle 진동 저감을 위한 차체 구조 최적 설계
이원태(Wontae Lee),김영현(Younghyun Kim) 한국소음진동공학회 2014 한국소음진동공학회 학술대회논문집 Vol.2014 No.10
A diesel forklift truck under 3-ton class has disadvantages in the vibration transmission path. Because the weight ratio of body structure to powertrain which is source of excitation force is lower th an a midclass forklift. In addition, the torsional and bending vibration mode frequencies of body structure are within the engine excitation frequency range, then high idle vibration generated by resonance. In this paper vehicle body structure design and optimization technique considering idle vibration reduction are presented. Design sensitivity analysis is applied to search the sensitive of design parameters in body structure. The design parameters such as thickness and pillar cross section were optimized to increase the torsional and bending vibration mode frequencies.