新梢頂端培養에 의한 대추나무(Zizyphus jujuba Miller)의 callus로부터 植物體 再分化

宋沅燮,金鎭洙,劉成吾 圓光大學校大學院 1991 論文集 Vol.8 No.-

Organogenesis from callus induced from shoot tip in vitro cultured was examined with using different strength of MS medium containing various concentration of different plant growth regulators alone and its combination in jujube (Zizyphus jujuba Miller). Callus induction was favorable in the treatment of concentration of 2,4-D 1.0, 0.3mg/1 adding on MS medium. Favorable shoot proliferation from callus subcultured on medium for shooting appeared in the treatment of 0.5mg/1 BAP alone adding on 1/2 MS medium. For rooting induction 1/2 MS medium supplemented with the combination of 0.5 or 1.5mg/1 IBA and 0.1, 1.0mg/1 BAP was most effective but 1/2 MS medium containing IBA alone had little effect. Keywords:organogenesis;shoot;callus;jujube MS=Murashige and Skoog Abbreviations:2,4-D=2,4-dichlorophenoxyacetic acid BAP=6-benzylamino purine IBA=indol-3-butylic acid

Constituents Analysis of Amino Acid and Antioxidative Activity from Cultivated Callus and Rhizome in Rhodiola sachalinensis

Song,Won-Seob,Chi,Hyung-Joon,Rim,Yo-Sup,Yoon,Jae-Ho 한국자원식물학회 2002 Plant Resources Vol.5 No.1

The material of Rhodiola sachatinensis collected from an alpine region of the west-northern China. For analysing the effect, 1 used Rhodiola sachatinensis s rhizome and cultivated callus. In EtOAc, BuOH, H₂0separation the plant showed strong antioxidative activity, but not in Hexane. The radical scavenging effect of EtOAc(RC50, 35(g), BuOH(RC50, 43(g), H₂0(RC50, 50(g) fraction and MeOH extract(RC50, 50(g) of the Rhodiola sachatinensis was comparable to that of synthetic antioxidant BHA(RC50, 14(g) and α-Tocopherol(RC50, 12(g). Total amino acid concentration of plant of In nature condition were 18,009ppm, and major components were arginine, glutamic acid, aspartic acid and valine. The ratio of essential/total amino acid on plant of In nature condition was 46.93%. Total amino acid concentration of callus of In vitro condition were 32,435ppm, and major components were valine, histidine, lysine and leucine. The ratio of essential/total amino acid on callus of In vitro condition was 56.07%. was 56.07%.

茴香(Foeniculum vulgare G.)의 器內增殖 : 1. Embryogenic Callus 誘起 및 器內不定胚 發生에 미치는 植物生長調節物質의 效果 1. Effect of Plant Growth Regulators on Embryogenic Callus Induction and Somatic Embryogenesis

宋沅燮,朴仁鉉,丁蓮圭 順天大學校 農業科學硏究所 1990 農業科學硏究 Vol.4 No.-

Young stem and leaf segment of Foeniculum vulgare for producing embryologic callus and embryos were cultured in vitro on various medium as well as MS medium treated with different concentration of plant growth regulators alone or in combination with other plant growth on MS medium, whereas was lower in treatments with BAP and kinetin. The combination effect with 2,4-D 0.01㎎/ℓ and cytokinins varied with kind of cytokinins. The plot of BAP added with 2,4-D 0.01㎎/ℓ was effective as high as 2,4-D alone treatment. MS and MT medium were more effective than LS and W medium in production of somatic embryos from incubated embryologic callus. Most favorable plant growth regulator for producing somatic embryos was 2,4-D 0.01㎎/ℓ but IAA, ABA, BAP, and kinetin were less effective at any concentration. BAP 0.01㎎/ℓ added with 2,4-D 0.1㎎/ℓ effective as high as 2,4-D 0.1㎎/ℓ alone in somatic embryo production.

기내배양과 자연조건에서의 유자(Citrus junos Sieb. et Tanaka)의 부정배 발생의 특징

송원섭,진영욱,김동철,우 서 順天大學校 1997 論文集 Vol.16 No.1

유자(Citrus junos)의 부정배는 자연조건에서는 주심조직과 배낭근처에서 발생하였으며 부정배로 발육될 시원세포들은 매우 큰 핵과 세포질로 충만되어 있고 주로 배낭근처에 있는 주공측의 주심조직에서 발생되었다. 기내배양 조건에서는 자연조건에서 보다도 훨씬 많은 부정배들이 배낭내에서 관찰되었으며 이러한 부정배들은 대부분 배낭과 밀접해 있는 주심세포층으로부터 발생하였다. 또한 몇몇 부정배들은 주심조직에서 직접 발생하는 것이 관찰되었다. Adventive embryo usually occurs inside the embryos ac nucellar tissue in the in Vivo state. Initial cells to be developed in to adventive embryos were conspicuous with large nucleus and dense cytoplasm, and arose mainly from nucellar tissue or from integuments. In the in Vitro culture, much more embryos were observed in the embryo sac. than in the in Vivo conditions, and embryos seemed to arise from the nucellar cell layer lining the embryo sac. Some of the adventive embryos were observed occurring directly from the nucellar.

백양꽃(Lycoris koreana) × 개상사화(Lycoris aurea) 교잡 미숙배로부터 식물체 생산

송원섭,박인현,박진형 圓光大學校大學院 1992 論文集 Vol.10 No.-

This experiment was conducted to investigate cultural conditions of immature embryo in Lycoris koreana×Lycoris aurea hybrid. Optimum concentration of ager supplemented to Murashige and Tucker(MT) medium for plantlet production was 6g/l. Optimum concentration of sucrose supplemented to MT medium for plantlet production was 30~50 g/l. For plantlet prodution, MT medium supplemented with NAA 0.01 mg/l plus zeatin 0.1~0.01mg/l and without olant growth regulator was most effective. Optimum level of PH controlled to MT medium for plantlet productiom was 4.5~5.0. Key words : Lycoris koreana, Lycoris aurea, immature embryo, plantlet production.

茴香(Foeniculum vulgare G.)의 器內增殖 : 2. 몇가지 유기물과 활성탄의 첨가가 器內不定胚 發生에 미치는 效果 2. Effect of Complex Addenda and Activated Charcoal on somatic Embrogenesis

宋源燮,朴仁鉉,丁蓮圭 順天大學校 農業科學硏究所 1991 農業科學硏究 Vol.5 No.-

Somatic embryogenesis from incubated embryogenic callus in vitro cultured stem and leaf segment of Foeniculum vulgare promoted with complex addenda(coconut milk, yeast extract, malt extract and sucrose) as well as sucrose added on MS medium containing 2,4-D 0.01㎎/ℓ or not. Treatment of coconut milk 50㎎/ℓ was effective, otherwise the higher concentration decreased production of somatic embryos. Up to 300㎎/ℓ of yeast extract increased embryogenesis rate but 500㎎/ℓ treatment severely dropped the rate. Malt extract and sucrose increased proportionally the rate of somatic embryogenesis with higher concentration, up to 87% in malt extract 500㎎/ℓ and 93% in sucrose 50g/ℓ when 2,4-D 0.01㎎/ℓ was added on the medium. Activated charcoal treatment decreased remarkably the rate of somatic embryogenesis. In all treatment the adding plots of 2,4-D 0.01㎎/ℓ was higher than the non-adding plots in somatic embryogenesis.

Plant Regeneration and Bulblet Formation of Allium wakegi Araki

Song,Won-seob,Yang,Deok-Chun,Yoon,Jae-Ho,Ryu,Sang-Hyun 한국자원식물학회 2004 Plant Resources Vol.7 No.1

Allium wakegi was cultured shoot tip in the condition of light culture. The Allium wakegi added plant growth regulator was observed of plant regeneration and bulblet formation. Callus Induction and growing rate was the best of 78% when added alone 2,4-D 0.5mg/L. In the formation of shoot, its regeneration rate was 96% when added BA 0.5mg/L in the light culture condition. When BA 0.5mg/L and NAA 0.5mg/L mixed and BA 0.5 mg/L and NAA 1.0mg/L mixed, the rates were 99% and 97% respectively, and these conditions were suitable for forming shoot. In the formation of roots, when added NAA 2.0mg/L in the light culture condition, the regeneration rate was 90.6 % and the roots were abnormal. When added NAA 1.0mg/L, the rate was 82 % and the highest. In the formation of bulbs, when BA 05mg/L and NAA 1.0mg/L mixed, the root generantion and its size in the bulbs was the best compare to other treatment experiments.

Effect of Drying Agents and Ethyl Alcohol on Change of Flower Color of Gentian (Gentiana uchiyamai) in Pressed Flower

Song,Won-Seob,Kim,Young-Sun 한국자원식물학회 2002 Plant Resources Vol.5 No.2

The effects of drying agents and ethyl alcohol treatment on the flower color of Gentiana uchiyamai. The flower color was not much changed in flower sheet, in the case of absorbtion papers only. The color was least changed in Korean and China hwasunjis, in the case of absorbtion papers and silica gel treatment. The color was less changed in flower sheets, window papers, and China hwasunjis, and was most changed in newspapers, in the case of absorbtion papers and Na2SO4 수식 이미지 treatment. The color was least changed in China hwasunjis, and most changed in dampened papers, in the case of absorbtion papers and CaO treatment. The color tended to change far from the original one, as treatment time got longer, in the case of 0.01M and 0.lM of tartaric acid. The longer the treatment time and the bigger the concentration of ethyl alcohol, the bigger flower color.

Effects of Electric Stimulation with Static Stretching on Hamstrings Flexibility

( Won-min Song ),( Hye-jeong Seo ),( Won-seob Shin ) 대한물리치료학회 2015 대한물리치료학회지 Vol.27 No.3

Purpose: Flexibility has been considered one of the most important goals in rehabilitation. This study aimed to investigate the effect of transcutaneous electrical nerve stimulation (TENS) with the static stretching technique on the flexibility of hamstrings. Methods: Twenty-four subjects (15 men, 9 women) with limited hamstrings flexibility received three different intervention sessions in random order. The treatment sessions included static stretching (SS), static stretching with motor-level TENS (SS with motor TENS) and sensory-level TENS (SS with sensory TENS). All sessions of SS were performed in the straight-leg raise position for 30 seconds followed by rest for 15 seconds, in repetitions for 10 minutes using a belt. The TENS groups underwent TENS stimulation (40 μs, 100 Hz) during the stretching for 10 minutes. Outcome measures were evaluated according to active knee extension (AKE) and recorded before the session and at 0, 3, 6, 9, and 15 minutes after the session. Results: There was significant improvement in hamstrings flexibility within all groups (p<0.05). SS with TENS (both sensory and motor) maintained significant increases in knee extension range of motion until 15 minutes post-treatment. In contrast, the SS-only group maintained significantly increased hamstrings flexibility only until 6 minutes post-treatment (p<0.05). Conclusion: Improvement in hamstrings flexibility with SS with TENS was maintained longer than with SS-only intervention. Electrical stimulation with stretching may be more effective than SS alone for increased duration of maintained hamstrings flexibility.

Effects of Electric Stimulation with Static Stretching on Hamstrings Flexibility

Song, Won-Min,Seo, Hye-Jeong,Shin, Won-Seob The Korean Society of Physical Therapy 2015 대한물리치료학회지 Vol.27 No.3

Purpose: Flexibility has been considered one of the most important goals in rehabilitation. This study aimed to investigate the effect of transcutaneous electrical nerve stimulation (TENS) with the static stretching technique on the flexibility of hamstrings. Methods: Twenty-four subjects (15 men, 9 women) with limited hamstrings flexibility received three different intervention sessions in random order. The treatment sessions included static stretching (SS), static stretching with motor-level TENS (SS with motor TENS) and sensory-level TENS (SS with sensory TENS). All sessions of SS were performed in the straight-leg raise position for 30 seconds followed by rest for 15 seconds, in repetitions for 10 minutes using a belt. The TENS groups underwent TENS stimulation ($40{\mu}s$, 100 Hz) during the stretching for 10 minutes. Outcome measures were evaluated according to active knee extension (AKE) and recorded before the session and at 0, 3, 6, 9, and 15 minutes after the session. Results: There was significant improvement in hamstrings flexibility within all groups (p<0.05). SS with TENS (both sensory and motor) maintained significant increases in knee extension range of motion until 15 minutes post-treatment. In contrast, the SS-only group maintained significantly increased hamstrings flexibility only until 6 minutes post-treatment (p<0.05). Conclusion: Improvement in hamstrings flexibility with SS with TENS was maintained longer than with SS-only intervention. Electrical stimulation with stretching may be more effective than SS alone for increased duration of maintained hamstrings flexibility.