The complete mitochondrial genome of a threatened loach (Sinibotia reevesae) and its phylogeny

Yuan‑Chao Zou,Bi‑Wen Xie,Chuan‑Jie Qin,Yong‑Ming Wang,Deng‑Yue Yuan,Rui Li,Zheng‑Yong Wen 한국유전학회 2017 Genes & Genomics Vol.39 No.7

In present study, the complete mitochondrial genome of Sinibotia reevesae was first sequenced using the next-generation sequencing technology and annotated using bioinformatic tools. The circular mitochondrial genome was 16,572 bp in length, and contained 13 proteincoding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and 1 displacement loop locus. It presents a typical gene organization and order for completely sequenced cypriniformes mitogenomes. The control region could be divided into three parts included the extended termination associated sequence domain, the central conserved domain and the conserved sequence block. Interestingly, two stemloop domains were found in control region and OL region, respectively. Furthermore, phylogenetic analyses using concatenated amino acid and nucleotide sequences of the 13 protein-coding genes with two different methods (Maximum likelihood and Bayesian analysis) both highly supported the close relationship of S. reevesae and Sinibotia superciliaris, which was in line with the previous classifications based on morphological and molecular studies. These data provide useful information for a better understanding of the mitogenomic diversities and evolution in fish as well as novel genetic markers for studying population genetics and species identification.

Metabolism of α-Actin, Expression of α-Actin mRNA and MHC mRNA Induced by Exhaustive Eccentric Exercise in Skeletal Muscles of Rats and Effects of Acupuncture on Them

Wang, Rui Yuan,Yuan, Yu He,Zhang, Wen Dong,Zhao, Lin,Xun, Chuu Yan 한국체육무용국제교류학회 2001 한국체육무용국제교류학회지 Vol.7 No.-

The purpose of this study is to investigate the changes of α-actin metabolism, α-actin mRNA and MHC mRNA expression in skeletal muscles of rats after one set exhaustive eccentric exercise and the effects of acupuncture on them. 130 adult male SD rats were randomly divided into the sedentary group and the exercise group. The rats of the exercise group were again divided into the acupuncture group and non-acupuncture group. According to the sampling time, the exercise group (the acupuncture and non-acupuncture group) was divided into 12 groups, that is the 0 hours, 6 hours, 12 hours, 24 hours, 48 hours and 72 hours groups. In each group there were ten rats. The rats weighting 205±23.3 (mean±SE) were maintained at 23±1℃. They were fed in different chests taking food and drink freely. The rats of the exercise group carried out continuous downhill running with the speed of 16m/min for about 4 hours until exhaustion on the animal treadmill (degree -16°). The rats of the acupuncture group were treated with acupuncture immediately after exhaustive eccentric exercise, but those of the sedentary group and the non-acupuncture group were treated without acupuncture. They were the control groups. The muscle samples were taken immediately, 6 hours, 12 hours, 24 hours, 48 hours and 72 hours after exercise. The contents of G-actin, F-actin and T-actin in skeletal muscles of rats were measured by means of Dnase I assay. The gene expression of α-actin m RNA and MHC isoforms mRNA were determined by using the quantitative reverse transcription PCR technology. Compared with the sedentary group, there was no remarkable change of T-actin after exercise. The decrease G-actin could be detected only immediately after exercise. The content of F-actin was significantly higher than that of the sedentary group 0~6 hours after exercise, but 12~24 hours after exercise it decreased to a lower level than that of the sedentary group. A delayed increase of F-actin depolymerization presented after exercise. The F-actin value of the acupuncture group was improved in a lower range than that of the non-acupuncture group 0~6 hours after exercise, but 12~24 hours after exercise it was remarkably higher than those of the non-acupuncture group. It was shown that acupuncture could regulate the F-actin concentration in skeletal muscles of rats after exercise. Furthermore, the regulation might be bilateral. Acupuncture could stop the increase of F-actin delayed depolymerization induced by high loading exercise. The gene expression of α-actin mRNA in skeletal muscles of rats decreased significantly after one set exhaustive exercise. The duration of its recovery was longer than 72 hours. It might be that acupuncture accelerated the recovery process of α-actin mRNA expression after exercise. The MHC mRNA expression of the non-acupuncture group was lower than that of the sedentary group 6~12 hours after exercise. There appeared a delayed excess expression of MHC mRNA 48 hours after exercise. The curve of the change of the total MHC mRNA expression in the acupuncture groups was similar to that in the non-acupuncture group. The duration of recovery of the acupuncture group was shorter, the delayed excess expression of MHC mRNA was ahead of time and its range was higher than that of non-acupuncture group. It was shown that acupuncture could promote the recovery of MHC mRNA expression after exercise of high load

Hollow-structured Pd/TiO2 as a dual functional photocatalyst for methyl orange oxidation and selective reduction of nitrate into nitrogen

Wen-Min Wang,Shi-Jer Tseng,Yu-Shuan Huang,Qian-Yuan Wu,Wen-Long Wang,Jerry J. Wu 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.119 No.-

Nitrate pollution in wastewater has posed a threat to human health and the environment. Photocatalyticreduction is a promising technology to remove nitrate due to its high adaptability, low cost, and high efficiency. However, there is a great challenge to design photocatalyst with high nitrate reduction and highselectivity of nitrogen (N2) yield. In this study, a novel hollow-structured Pd/TiO2 (PHT1) photocatalyst issynthesized with large specific surface area (SBET) and pore volume, strong UV adsorption, high reductionpotential than nitrate to nitrite, high charge transfer rate, and low electron-hole recombination rate. PHT1 exhibits a superior photocatalytic activity on the removal of methyl orange and nitrate. In addition,PHT1 exhibits high N2 selectivity (>95 %). A lower pH can promote the reduction of nitrate and the selectivityof N2 by PHT1. Electrons (e-) and CO2– are the main active species for nitrate reduction with OH asauxiliary radicals. Three main pathways for nitrate reduction are proposed: i) the reaction with formicacid under acidic conditions; ii) the reduction by electrons and the loaded Pd of photocatalyst to captureelectrons to inhibit electron-hole recombination; iii) the reduction by CO2 – generated from formic acidoxidation by OH.

Identification of a Novel Human Lysophosphatidic Acid Acyltransferase, LPAAT-theta, Which Activates mTOR Pathway

( Wen Wen Tang ),( Jian Yuan ),( Xin Ya Chen ),( Xiu Ting Gu ),( Kun Tian Luo ),( Jie Li ),( Bo Wan ),( Ying Li Wang ),( Long Yu ) 생화학분자생물학회 2006 BMB Reports Vol.39 No.5

Functional Evaluation of Transplanted Kidneys with Reduced Field-of-View Diffusion-Weighted Imaging at 3T

Yuan Xie,Yanjun Li,Jiqiu Wen,Xue Li,Zhe Zhang,Jianrui Li,Yan’e Zhao,Peng Wang,Jun Zhang,Ying Tian,Long Jiang Zhang,Guang Ming Lu 대한영상의학회 2018 Korean Journal of Radiology Vol.19 No.2

Objective: To determine the feasibility of reduced field-of-view diffusion-weighted imaging (rFOV DWI) with multi-b values to detect functional variability in transplanted kidneys. Materials and Methods: Using a 3T MRI scanner, multi-b rFOV DWI of transplanted kidney or native kidney was performed in 40 renal transplantation recipients and 18 healthy volunteers. The patients were stratified, according to an estimated glomerular filtration rate (eGFR): Group 1, eGFR ≥ 60 mL/min/1.73 m2; Group 2, eGFR ≥ 30 mL/min/1.73 m2 and < 60 mL/min/1.73 m2; Group 3, eGFR < 30 mL/min/1.73 m2. Total apparent diffusion coefficient (ADCT), perfusion-free ADC (ADCD) and perfusion fraction (FP) of kidneys were calculated and compared among the four groups. Correlations between the imaging results and eGFR were assessed. Results: All volunteers had eGFR ≥ 60 mL/min/1.73 m2, while 16, 16, and 8 patients were included in Groups 1, 2, and 3, respectively. In the renal cortex, ADCT was higher in Group 1 ([1.65 ± 0.13] x 10-3 mm2/s) than Group 3 ([1.44 ± 0.11] x 10-3 mm2/s) (p < 0.05), and the inter-group differences of FP values were significant (all p < 0.05) (0.330 ± 0.024, 0.309 ± 0.019, 0.278 ± 0.033, and 0.250 ± 0.028 for control group, Groups 1, 2, and 3, respectively). Renal cortical ADCT, ADCD, FP, and renal medullary ADCT and FP correlated positively with eGFR (r = 0.596, 0.403, 0.711, 0.341, and 0.323, respectively; all p < 0.05). When using 0.278 as the cutoff value, renal cortical FP had a sensitivity of 97.1% and a specificity of 66.7% for predicting decreased renal function. Conclusion: Multi-b rFOV DWI presents transplanted kidneys with high resolution, which is a promising functional tool for non-invasively monitoring function of transplanted kidneys.

A Novel All-trans Retinoid Acid Derivative Induces Apoptosis in MDA-MB-231 Breast Cancer Cells

Wang, Bei,Yan, Yun-Wen,Zhou, Qing,Gui, Shu-Yu,Chen, Fei-Hu,Wang, Yuan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

Aims: To explore the effect and probable mechanism of a synthetic retinoid 4-amino-2-tri-fluoromethylphenyl ester (ATPR) on apoptosis of MDA-MB-231 breast cancer cells. Materials and Methods: MTT assays were performed to measure the proliferation of MDA-MB-231 cells treated with different concentrations of all-trans retinoic acid (ATRA) and ATPR. Morphologic changes were observed by microscopy. The apoptosis rates and cell cycling of MDA-MB-231 cells treated with ATRA or ATPR were assessed using flow cytometry analysis. Expression of retinoic acid receptor and phosphorylation of ERK, JNK, p38 proteins were detected by Western blotting. Results: Treatment of the cells with the addition of $15{\mu}mol/L$ ATPR for 48 h clearly demonstrated reduced cell numbers and deformed cells, whereas no changes in the number and morphology were observed after treatment with ATRA. The apoptosis rate was 33.2% after breast cancer MDA-MB-231 cells were treated by ATPR ($15{\mu}mol/L$) whereas ATRA ($15{\mu}mol/L$) had no apoptotic effect. ATPR inhibited the phosphorylation of ERK, JNK, and p38 while ATRA had no significant effect. ATPR inhibited the expression of BiP and increased the expression of Chop at the protein level compared with control groups, ATRA and ATPR both decreased the protein expression of $RXR{\alpha}$, ATPR reduced the protein expression of $RAR{\beta}$ and $RXR{\beta}$ while ATRA did not decrease $RAR{\beta}$ or $RXR{\beta}$. Conclusions: ATPR could induce apoptosis of breast cancer MDA-MB-231 cells, possible mechanisms being binding to $RAR{\beta}/RXR{\beta}$ heterodimers, then activation of ER stress involving the MAPK pathway.

Chemical and Pharmacological Studies of Saponins with a Focus on American Ginseng

Yuan, Chun-Su,Wang, Chong-Zhi,Wicks, Sheila M.,Qi, Lian-Wen The Korean Society of Ginseng 2010 Journal of Ginseng Research Vol.34 No.3

Asian ginseng (Panax ginseng) and American ginseng (Panax quinquefolius L.) are the two most recognized ginseng botanicals. It is believed that the ginseng saponins called ginsenosides are the major active constituents in both ginsengs. Although American ginseng is not as extensively studied as Asian ginseng, it is one of the best selling herbs in the US, and has garnered increasing attention from scientists in recent years. In this article, after a brief introduction of the distribution and cultivation of American ginseng, we discuss chemical analysis of saponins from these two ginsengs, i.e., their similarities and differences. Subsequently, we review pharmacological effects of the saponins, including the effects on the cardiovascular system, immune system, and central nervous system as well as the anti-diabetes and anti-cancer effects. These investigations were mainly derived from American ginseng studies. We also discuss evidence suggesting that chemical modifications of ginseng saponins would be a valuable approach to develop novel compounds in drug discovery.

Bacterial Fruit Rot of Apricot Caused by Burkholderia cepacia in China

Yuan Fang,Bin Li,Fang Wang,Baoping Liu,Zhiyi Wu,Ting Su,Wen Qiu,Guanlin Xie 한국식물병리학회 2009 Plant Pathology Journal Vol.25 No.4

An unreported disease of apricot was observed in orchards in Zhejiang province, China. Symptoms started as water soaked lesions on the fruit surface. Later, water-soaked areas developed and spread to the entire fruit, resulting in soft rot of the whole fruit. The causal organism isolated from symptomatic fruits was identified as Burkholderia cepacia based on its biochemical and physiological characteristics and confirmed by the cellular fatty acid composition and Biolog data as well as 16S rRNA gene sequence analysis. The bacterial isolates caused similar symptoms when inoculated onto fruits of apricot. In addition, European plum, Japanese plum, nectarine and kiwifruit were susceptible to the B. cepacia pathogen. However, the B. cepacia pathogen failed to cause any visible symptoms when it was inoculated onto 16 other fruits. This is the first report of a bacterial disease of apricot caused by B. cepacia in China.

DIAGNOSTIC VALUE OF ADRENAL SCINTIPHOTOGRAPHY IN ADRENAL DISEASES

Wang, Pei Wen,Huang, Miau Ju,Tzen, Kai Yuan,Su, Cheng Daw 대한핵의학회 1984 핵의학 분자영상 Vol.18 No.1

Novel HMW glutenin genes from Aegilops tauschii and their unique structures

Wen-Jie Chen,Zhong-Wei Yuan,Lian-Quan Zhang,Xing Fan,Ze-Hong Yan,Ji-Rui Wang,You-Liang Zheng,Huai-Gang Zhang,Deng-Cai Liu 한국유전학회 2012 Genes & Genomics Vol.34 No.3

A pair of novel high-molecular-weight glutenin subunits (HMW-GS) 1Dx5.3t and 1Dy12.1**t were revealed and characterized from Ae. tauschii accession PI554324. SDS-PAGE band of 1Dx5.3t was between those of 1Bx6 and 1Bx7, while 1Dy12.1**t with slightly faster migration rate than that of 1Dy12. The lengths of 1Dx5.3t and 1Dy12.1**t were 2115 bp and 1986 bp, encoding 703 and 660 amino acid residues,respectively. Their authenticity was confirmed by successful expression of the coding regions in Escherichia coli. 1Dx5.3t is the shortest of the known Dx-type alleles. 1Dy12.1**t is also a special subunit since it has an additional cysteine in the front of the central repetitive domain. This cysteine that is not existed in previously reported Dy-type genes may be useful for improving bread wheat quality. Median-joining Network analysis indicated that 1Dy12.1**t may be a key site in the genealogy of the Glu-Dy.