유기질비료 시비량에 따른 오미자 묘목의 초기 생육특성에 관한 연구

이경아,박태호,박성용,김도현,오문국,김민수,안태진,이상원,김영국,안영섭,박충범,차선우,송범헌 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.1

This study was carried out to have basic and applied informations to find the optimum nitrogen fertilization level for organic cultivation and to investigate early growth characteristics of Schizandra chinensis Baillon. Different application levels of the organic fertilizer were based on the recommended rate of N fertilizer. Five different levels of the organic fertilizer were applied to S. chinensis and early growth responses such as the length of vine, the diameter of vine, the number of leaves, and the number of branches were investigated. Lengths of main and total vines were shown longer at the level of 1.0 time organic fertilizer than those at others. The vine length at early growth was the highest at the level of 1.0 time organic fertilizer among the treatments. Stem diameter was shown thicker level of 1.0 time organic fertilizer than others on August and September, whereas on October it was the thickest in the conventional cultivation. Number of leaf was increased higher at the level of 1.0 and 2.0 times organic fertilizer than that in the compared to conventional. The number of branches was appeared the lowest at the level of 1.0 time organic fertilizer at early growth, however they were increased through progressing their growth stages. They were not significantly difference among the treatments except for the level of 2.0 times organic fertilizer. Based on the research results, the growth of S. chinensis was commonly higher at the level of 1.0 time organic fertilizer compared to other treatments.

Thymic stromal lymphopoietin–activated invariant natural killer T cells trigger an innate allergic immune response in atopic dermatitis

Wu, Wen Hao,Park, Chang Ook,Oh, Sang Ho,Kim, Hee Jung,Kwon, Yeon Sook,Bae, Byung Gi,Noh, Ji Yeon,Lee, Kwang Hoon Elsevier 2010 The Journal of allergy and clinical immunology Vol.126 No.2

<P><B>Background</B></P><P>Although invariant natural killer T (iNKT) cells have been shown to play a critical role in the pathogenesis of asthma, the role of iNKT cells in atopic dermatitis (AD) has not been well evaluated.</P><P><B>Objective</B></P><P>We investigated whether iNKT cells in patients with AD increased and whether iNKT cells were activated by thymic stromal lymphopoietin (TSLP), which is highly expressed in keratinocytes of AD.</P><P><B>Methods</B></P><P>We assessed the population of iNKT cells in PBMCs of patients with AD and healthy controls (HCs) using flow cytometry. Immunohistochemistry was used to evaluate iNKT cells and TSLP expression in AD and HC skin. We also evaluated whether iNKT cells expressed the TSLP receptor, the effects of TSLP on iNKT cells, and iNKT cell–dendritic cell interactions in a TSLP-rich environment.</P><P><B>Results</B></P><P>There were more iNKT cells among PBMCs of patients with moderate to severe AD than mild AD (<I>P</I> < .05) and HC (<I>P</I> < .001). The number of iNKT cells was significantly larger in severe AD skin lesions than in mild (<I>P</I> < .001) or moderate AD skin lesions (<I>P</I> < .05). TSLP expression increased in lesional skin (<I>P</I> < .001) but not in the sera of patients with AD (<I>P</I> = .729) compared with HC. iNKT cells expressed TSLP receptor protein and mRNA. TSLP directly activated iNKT cells to secrete IL-4 and IL-13, and the concurrent addition of dendritic cells further activated IFN-γ expression.</P><P><B>Conclusion</B></P><P>Increased iNKT cells activated by TSLP, especially in patients with severe AD, might play an essential role in the innate allergic immune response in AD.</P>

Legumain Protein as a Potential Predictive Biomarker for Asian Patients with Breast Carcinoma

Wu, Mei,Shao, Guang-Rui,Zhang, Fei-Xue,Wu, Wen-Xiu,Xu, Ping,Ruan, Zheng-Min Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

Background: Treatment for breast cancer is mainly performed by surgical resection of primary tumors and chemotherapy. However, after tumor invasion and metastases, breast cancer is hard to control. Clarification of the pathogenic mechanisms would be helpful to the prognosis or therapy for the breast cancer. The aim of this study is to investigate the clinical and prognostic implications of legumain protein Materials and Methods: In this study, we examined mastectomy specimens from 114 breast cancer and matching, 26 adjacent non-cancerous tissues using immunohistochemistry. Results: The results indicated that positive expression of legumain protein in breast cancer was 51.8 % (59/114) and the positive expression of legumain protein in adjacent non-cancerous tissue was 11.5% (3/26). It appeared to be related with lymph node metastasis of breast cancer (p=0.02) and correlation analysis indicated that legumain expression was correlated positively with the estrogen receptor (ER) and mutant-type p53 expression (both p<0.05). Positive legumain expression was significantly associated with shorter overall survival time in breast cancer patients (log-rank p<0.01). Multivariate survival analysis suggested that the positive legumain expression was an independent predictor of poorer overall survival in patients with breast cancer (HR=0.24; 95%CI 0.11-0.65, p=0.03). Conclusions: Legumain might be a new potential biomarker for breast cancer, which may reflect the prognosis and overall survival.

From an Authoritarian Party-State to a Democratic and Competitive Party System

Wu, Wen Cheng 서강대학교 동아연구소 1988 東亞 硏究 Vol.- No.15

Identification of novel rheumatoid arthritis-associated MiRNA-204-5p from plasma exosomes

Wu Long-Fei,Zhang Qin,Mo Xing-Bo,Lin Jun,Wu Yang-Lin,Lu Xin,He Pei,Wu Jian,Guo Yu-Fan,Wang Ming-Jun,Ren Wen-Yan,Deng Hong-Wen,Lei Shu-Feng,Deng Fei-Yan 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Rheumatoid arthritis (RA) is an autoimmune disease characterized by infiltration of immune cells in the synovium. However, the crosstalk of immune cells and synovial fibroblasts is still largely unknown. Here, global miRNA screening in plasma exosomes was carried out with a custom microarray (RA patients vs. healthy controls = 9:9). A total of 14 exosomal miRNAs were abnormally expressed in the RA patients. Then, downregulated expression of exosomal miR-204-5p was confirmed in both the replication (RA patients vs. healthy controls = 30:30) and validation groups (RA patients vs. healthy controls = 56:60). Similar to the findings obtained in humans, a decreased abundance of exosomal miR-204-5p was observed in mice with collagen-induced arthritis (CIA). Furthermore, Spearman correlation analysis indicated that plasma exosomal miR-204-5p expression was inversely correlated with disease parameters of RA patients, such as rheumatoid factor, erythrocyte sedimentation rate, and C-reactive protein. In vitro, our data showed that human T lymphocytes released exosomes containing large amounts of miR-204-5p, which can be transferred into synovial fibroblasts, inhibiting cell proliferation. Overexpression of miR-204-5p in synovial fibroblasts suppressed synovial fibroblast activation by targeting genes related to cell proliferation and invasion. In vivo assays found that administration of lentiviruses expressing miR-204-5p markedly alleviated the disease progression of the mice with CIA. Collectively, this study identified a novel RA-associated plasma exosomal miRNA-204-5p that mediates the communication between immune cells and synovial fibroblasts and can be used as a potential biomarker for RA diagnosis and treatment.

Comparative Study of Bioactive Constituents in Crude and Processed Glycyrrhizae radix and Their Respective Metabolic Profiles in Gastrointestinal Tract In Vitro by HPLC-DAD and HPLC-ESI/MS Analyses

Wen-wu Huang,Xiaobo Li,Meng Yue Wang,Hai-ming Shi,Ying Peng,Chong-sheng Peng,Min Zhang,Yue Li,Jing Lu 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.11

Two HPLC methods with diode array detection (HPLC-DAD) and electrospray ionization-mass spectrometry (HPLC-ESI/MS), respectively, were developed to investigate the differences of chemical constituents and their metabolism in gastrointestinal tract in vitro between two decoctions of crude and processed Glycyrrhizae radix. Total of eleven constituents (liquiritin apioside, liquiritin, licuraside, isoliquiritin, ononin, glycyrrhizin, liquiritigenin-7,4'-diglucoside, licorice saponin A3, 22β-acetoxylglycyrrhizic acid, licorice saponin G2, and yunganoside E2) were identified in the two decoctions, whereas lower contents of these constituents were usually found in the decoction of processed G. radix. Furthermore, these constituents were metabolized into their respective aglycons in human intestinal bacteria juice, and the metabolism ratios were all higher in processed G. radix decoction. No change was found in artificial gastric or intestinal juice. This study revealed that the processing can alter the contents of main constituents in crude G. radix and their metabolism in gastrointestinal tract, in which intestinal bacteria play an important role in the metabolism of licorice constituents.

Iterative Symbol Decoding of Variable-Length Codes with Convolutional Codes

Wu, Hung-Tsai,Wu, Chun-Feng,Chang, Wen-Whei The Korea Institute of Information and Commucation 2016 Journal of communications and networks Vol.18 No.1

In this paper, we present a symbol-level iterative source-channel decoding (ISCD) algorithm for reliable transmission of variable-length codes (VLCs). Firstly, an improved source a posteriori probability (APP) decoding approach is proposed for packetized variable-length encoded Markov sources. Also proposed is a recursive implementation based on a three-dimensional joint trellis for symbol decoding of binary convolutional codes. APP channel decoding on this joint trellis is realized by modification of the Bahl-Cocke-Jelinek-Raviv algorithm and adaptation to the non-stationary VLC trellis. Simulation results indicate that the proposed ISCD scheme allows to exchange between its constituent decoders the symbol-level extrinsic information and achieves high robustness against channel noises.

Use of Magnetic Resonance Imaging for Evaluating Residual Breast Tissue After Robotic-Assisted Nipple-Sparing Mastectomy in Women With Early Breast Cancer

Wu Wen-Pei,Lai Hung-Wen,Liao Chiung-Ying,Lin Joseph,Huang Hsin-I,Chen Shou-Tung,Chou Chen-Te,Chen Dar-Ren 대한영상의학회 2023 Korean Journal of Radiology Vol.24 No.7

Objective: Prospective studies on postoperative residual breast tissue (RBT) after robotic-assisted nipple-sparing mastectomy (R-NSM) for breast cancer are limited. RBT presents an unknown risk of local recurrence or the development of new cancer after curative or risk-reducing mastectomies. This study investigated the technical feasibility of using magnetic resonance imaging (MRI) to evaluate RBT after R-NSM in women with breast cancer. Materials and Methods: In this prospective pilot study, 105 patients, who underwent R-NSM for breast cancer at Changhua Christian Hospital between March 2017 and May 2022, were subjected to postoperative breast MRI to evaluate the presence and location of RBT. The postoperative MRI scans of 43 patients (age, 47.8 ± 8.5 years), with existing preoperative MRI scans, were evaluated for the presence and location of RBT. In total, 54 R-NSM procedures were performed. In parallel, we reviewed the literature on RBT after nipple-sparing mastectomy, considering its prevalence. Results: RBT was detected in 7 (13.0%) of the 54 mastectomies (6 of the 48 therapeutic mastectomies and 1 of the 6 prophylactic mastectomies). The most common location for RBT was behind the nipple-areolar complex (5 of 7 [71.4%]). Another RBT was found in the upper inner quadrant (2 of 7 [28.6%]). Among the six patients who underwent RBT after therapeutic mastectomies, one patient developed a local recurrence of the skin flap. The other five patients with RBT after therapeutic mastectomies remained disease-free. Conclusion: R-NSM, a surgical innovation, does not seem to increase the prevalence of RBT, and breast MRI showed feasibility as a noninvasive imaging tool for evaluating the presence and location of RBT.

Mode identifiability of a cable-stayed bridge under different excitation conditions assessed with an improved algorithm based on stochastic subspace identification

Wen-Hwa Wu,Sheng-Wei Wang,Chien-Chou Chen,Gwolong Lai 국제구조공학회 2016 Smart Structures and Systems, An International Jou Vol.17 No.3

Deficient modes that cannot be always identified from different sets of measurement data may exist in the application of operational modal analysis such as the stochastic subspace identification techniques in large-scale civil structures. Based on a recent work using the long-term ambient vibration measurements from an instrumented cable-stayed bridge under different wind excitation conditions, a benchmark problem is launched by taking the same bridge as a test bed to further intensify the exploration of mode identifiability. For systematically assessing this benchmark problem, a recently developed SSI algorithm based on an alternative stabilization diagram and a hierarchical sifting process is extended and applied in this research to investigate several sets of known and blind monitoring data. The evaluation of delicately selected cases clearly distinguishes the effect of traffic excitation on the identifiability of the targeted deficient mode from the effect of wind excitation. An additional upper limit for the vertical acceleration amplitude at deck, mainly induced by the passing traffic, is subsequently suggested to supplement the previously determined lower limit for the wind speed. Careful inspection on the shape vector of the deficient mode under different excitation conditions leads to the postulation that this mode is actually induced by the motion of the central tower. The analysis incorporating the tower measurements solidly verifies this postulation by yielding the prevailing components at the tower locations in the extended mode shape vector. Moreover, it is also confirmed that this mode can be stably identified under all the circumstances with the addition of tower measurements. An important lesson learned from this discovery is that the problem of mode identifiability usually comes from the lack of proper measurements at the right locations.

Stable modal identification for civil structures based on a stochastic subspace algorithm with appropriate selection of time lag parameter

Wu, Wen-Hwa,Wang, Sheng-Wei,Chen, Chien-Chou,Lai, Gwolong Techno-Press 2017 Structural monitoring and maintenance Vol.4 No.4

Based on the alternative stabilization diagram by varying the time lag parameter in the stochastic subspace identification analysis, this study aims to investigate the measurements from several cases of civil structures for extending the applicability of a recently noticed criterion to ensure stable identification results. Such a criterion demands the time lag parameter to be no less than a critical threshold determined by the ratio of the sampling rate to the fundamental system frequency and is firstly validated for its applications with single measurements from stay cables, bridge decks, and buildings. As for multiple measurements, it is found that the predicted threshold works well for the cases of stay cables and buildings, but makes an evident overestimation for the case of bridge decks. This discrepancy is further explained by the fact that the deck vibrations are induced by multiple excitations independently coming from the passing traffic. The cable vibration signals covering the sensor locations close to both the deck and pylon ends of a cable-stayed bridge provide convincing evidences to testify this important discovery.