BIOTIC CHANGES IN SOME AREAS OF EAST ASIA - ASSOCIATED RESPONSES TO NEOGENE EVOLUTION OF PACIFIC OCEAN GATEWAYS

( Wei Ning Wang ) 한국고생물학회 1997 Abstract volume Vol.- No.-

The Detection of Osthole and Application of Cell Imaging Based on Nitrogen-Doped Carbon Dots

Ning Wang,Xuefang Yang,Haojiang Wang,Liping Xie,Weihua Jia,Wei Bian,Martin M. F. Choi 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2020 NANO Vol.15 No.03

A fluorescence probe has been synthesized for the detection of osthole using the nitrogen-doped carbon dots (NCDs) as shown in Fig. 1. The NCDs were fully characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), x-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR). Under the optimal experimental conditions, the NCDs fluorescence probe was highly selective and sensitive to osthole. The linear response range for osthole was 5.0–7 5μM with a detection limit of 38 nM. The mechanism of the interaction of osthole and NCDs was discussed. The fluorescence probe has been applied to the analysis of biological samples. The as-synthesized NCDs with high fluorescence intensity, low toxicity and good biocompatibility were applied to cell imaging.

Wavelet-based automatic identification method of axle distribution information

Ning-Bo Wang,Wei-Xin Ren,Zhi-Wei Chen 국제구조공학회 2017 Structural Engineering and Mechanics, An Int'l Jou Vol.63 No.6

Accurately extracting the axle distribution information of a passing vehicle from bridge dynamic responses experiences a key and challenging step in non-pavement bridge weigh-in-motion (BWIM). In this article, the wavelet transformation is adopted and the wavelet coefficient curve is used as a substitute for dynamic response. The driving frequency is introduced and expanded to multi-axle vehicle, and the wavelet coefficient curve on specific scale corresponding to the driving frequency is confirmed to contain obvious axle information. On this basis, an automatic method for axle distribution information identification is proposed. The specific wavelet scale can be obtained through iterative computing, and the false peaks due to bridge vibration can be eliminated through cross-correlation analysis of the wavelet coefficients of two measure points. The integrand function that corresponds to the maximum value of the cross-correlation function is used to identify the peaks caused by axles. A numerical application of the proposed axle information identification method is carried out. Numerical results demonstrate that this method acquires precise axle information from the responses of an axle-insensitive structure (e.g., girder) and decreases the requirement of sensitivity structure of BWIM. Finally, an experimental study on a full-scale simply supported bridge is also conducted to verify the effectiveness of this method.

Surface Engineered CuO Nanowires with ZnO Islands for CO₂ Photoreduction

Wang, Wei-Ning,Wu, Fei,Myung, Yoon,Niedzwiedzki, Dariusz M.,Im, Hyung Soon,Park, Jeunghee,Banerjee, Parag,Biswas, Pratim American Chemical Society 2015 ACS APPLIED MATERIALS & INTERFACES Vol.7 No.10

<P>Large arrays of massively parallel (10<SUP>8</SUP> cm<SUP>–2</SUP>) CuO nanowires were surface engineered with dense ZnO islands using a few pulsed cycles of atomic layer deposition (ALD). These nanowires were subjected to UV–vis radiation-based CO<SUB>2</SUB> photoreduction under saturated humidity (CO<SUB>2</SUB> + H<SUB>2</SUB>O mixture) conditions. We monitored CO<SUB>2</SUB> to CO conversion, indicating the viability of these nanostructures as potential photocatalysts. High-resolution transmission electron microscopy and atomic force microscopy indicated an island growth mechanism of ZnO epitaxially depositing on pristine, single crystal CuO nanowire surface. Photoluminescence and transient absorption spectroscopy showed a very high density of defects on these ZnO islands which trapped electrons and enhanced their lifetimes. Peak CO conversion (1.98 mmol/g-cat/hr) and quantum efficiency (0.0035%) were observed in our setup when the ZnO islands impinged each other at 1.4 nm (8 cycles of ALD) diameter; at which point ZnO island perimeter lengths maximized as well. A mechanism whereby simultaneous H<SUB>2</SUB>O oxidation and CO<SUB>2</SUB> reduction occurred in the active perimeter region between CuO nanowire and ZnO islands is proposed to explain the observed photoconversion of CO<SUB>2</SUB> to CO.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2015/aamick.2015.7.issue-10/am508590j/production/images/medium/am-2014-08590j_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/am508590j'>ACS Electronic Supporting Info</A></P>

Mechanistic Analysis of Taxol-induced Multidrug Resistance in an Ovarian Cancer Cell Line

Wang, Ning-Ning,Zhao, Li-Jun,Wu, Li-Nan,He, Ming-Feng,Qu, Jun-Wei,Zhao, Yi-Bing,Zhao, Wan-Zhou,Li, Jie-Shou,Wang, Jin-Hua Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9

Objectives: To establish a taxol-resistant cell line of human ovarian carcinoma (A2780/Taxol) and investigate its biological features. Methods: The drug-resistant cell line (A2780/Taxol) was established by continuous stepwise selection with increasing concentrations of Taxol. Cell morphology was assessed by microscopy and growth curves were generated with in vitro and in vivo tumor xenograft models. With rhodamine123 (Rh123) assays, cell cycle distribution and the apoptotic rate were analyzed by flow cytometry (FCM). Drug resistance-related and signal associated proteins, including P-gp, MRPs, caveolin-1, PKC-${\alpha}$, Akt, ERK1/2, were detected by Western blotting. Results: A2780/Taxol cells were established with stable resistance to taxol. The drug resistance index (RI) was 430.7. Cross-resistance to other drugs was also shown, but there was no significant change to radioresistance. Compared with parental cells, A2780/Taxol cells were significantly heteromorphous, with a significant delay in population doubling time and reduced uptake of Rh123 (p<0.01). In vivo, tumor take by A2780 cells was 80%, and tumor volume increased gradually. In contrast, with A2780/Taxol cells in xenograft models there was no tumor development. FCM analysis revealed that A2780/Taxol cells had a higher percentage of G0/G1 and lower S phase, but no changes of G2 phase and the apoptosis rate. Expression of P-gp, MRP1, MRP2, BCRP, LRP, caveolin-1, PKC-${\alpha}$, Phospho-ERK1/2 and Phospho-JNK protein was significantly up-regulated, while Akt and p38 MARK protein expression was not changed in A2780/Taxol cells. Conclusion: The A2780/Taxol cell line is an ideal model to investigate the mechanism of muti-drug resistance related to overexpression of drug-resistance associated proteins and activation of the PKC-${\alpha}/ERK$ (JNK) signaling pathway.

Structural characterization of the pulmonary innate immune protein SPLUNC1 and identification of lipid ligands

Ning, Fangkun,Wang, Chao,Berry, Karin Zemski,Kandasamy, Pitchaimani,Liu, Haolin,Murphy, Robert C.,Voelker, Dennis R.,Nho, Chu Won,Pan, Choel-Ho,Dai, Shaodong,Niu, Liwen,Chu, Hong-Wei,Zhang, Gongyi The Federation of American Societies for Experimen 2014 The FASEB Journal Vol.28 No.12

<P>The short palate, lung and nasal epithelial clone 1 (SPLUNC1) protein is a member of the palate, lung, and nasal epithelium clone (PLUNC) family, also known as bactericidal/permeability-increasing (BPI) fold-containing protein, family A, member 1 (BPIFA1). SPLUNC1 is an abundant protein in human airways, but its function remains poorly understood. The lipid ligands of SPLUNC1 as well as other PLUNC family members are largely unknown, although some reports provide evidence that lipopolysaccharide (LPS) could be a lipid ligand. Unlike previous hypotheses, we found significant structural differences between SPLUNC1 and BPI. Recombinant SPLUNC1 produced in HEK 293 cells harbored several molecular species of sphingomyelin and phosphatidylcholine as its ligands. Significantly, <I>in vitro</I> lipid-binding studies failed to demonstrate interactions between SPLUNC1 and LPS, lipoteichoic acid, or polymyxin B. Instead, one of the major and most important pulmonary surfactant phospholipids, dipalmitoylphosphatidylcholine (DPPC), bound to SPLUNC1 with high affinity and specificity. We found that SPLUNC1 could be the first protein receptor for DPPC. These discoveries provide insight into the specific determinants governing the interaction between SPLUNC1 and lipids and also shed light on novel functions that SPLUNC1 and other PLUNC family members perform in host defense.—Ning, F., Wang, C., Berry, K. Z., Kandasamy, P., Liu, H., Murphy, R. C., Voelker, D. R., Nho, C. W., Pan, C.-H., Dai, S., Niu, L., Chu, H.-W., Zhang, G. Structural characterization of the pulmonary innate immune protein SPLUNC1 and identification of lipid ligands.</P>

Targeting treatment of bladder cancer using PTK7 aptamer-gemcitabine conjugate

Xiang Wei,Peng Yongbo,Zeng Hongliang,Yu Chunping,Zhang Qun,Liu Biao,Liu Jiahao,Hu Xing,Wei Wensu,Deng Minhua,Wang Ning,Liu Xuewen,Xie Jianfei,Hou Weibin,Tang Jin,Long Zhi,Wang Long,Liu Jianye 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Gemcitabine (GEM) is one of the first-line chemotherapies for bladder cancer (BC), but the GEMs cannot recognize cancer cells and have a low long-term response rate and high recurrence rate with side effects during the treatment of BC. Targeted transport of GEMs to mediate cytotoxicity to tumor and avoid the systemic side effects remains a challenge in the treatment of BC.Based on a firstly confirmed biomarker in BC-protein tyrosine kinase 7 (PTK7), which is overexpressed on the cell membrane surface in BC cells, a novel targeting system protein tyrosine kinase 7 aptamer-Gemcitabine conjugate (PTK7-GEMs) was designed and synthesized using a specific PTK7 aptamer and GEM through auto-synthesis method to deliver GEM against BC. In addition, the antitumor effects and safety evaluation of PTK7-GEMs was assessed with a series of in vitro and in vivo assays.PTK7-GEMs can specifically bind and enter to BC cells dependent on the expression levels of PTK7 and via the macropinocytosis pathway, which induced cytotoxicity after GEM cleavage from PTK7-GEMs respond to the intracellular phosphatase. Moreover, PTK7-GEMs showed stronger anti-tumor efficacy and excellent biosafety in three types of tumor xenograft mice models.These results demonstrated that PTK7-GEMs is a successful targeted aptamer-drug conjugates strategy (APDCs) to treat BC, which will provide new directions for the precision treatment of BC in the field of biomarker-oriented tumor targeted therapy.

Application on Web Page Filtering Technology

Bo Shen,Lei Li,Ning-wei Wang 보안공학연구지원센터 2014 International Journal of Multimedia and Ubiquitous Vol.9 No.12

Web page filtering technology intends to filter out the large number of the repeated and theme-unrelated noise information and obtain useful information. Some web filtering methods cannot make full use of the layout and visual features. In view of the new mainstream “DIV+CSS” designing style of modern commercial web sites, this paper summarizes that elements laying in the same div blocks have common semantic features and proposed a DIV_FOREST model to represent the web pages. And in combination with the Vision-based Page Segmentation Algorithm, a DVPS Algorithm which considers both layout features and visual features was proposed to improve web page filtering efficiency.

Influence of rub groove on rotordynamics associated with leakage air flow through a labyrinth seal

Wei Zhe Wang,Ying Zheng Liu,Guang Meng,Pu Ning Jiang 대한기계학회 2010 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.24 No.8

An extensive investigation of influence of rub grooves on dynamics and stability of the rotor, which is subjected to aerodynamic forcing associated with the leakage flow through 44 straight-through seals, was performed by using numerical calculations based on the single control-volume method and the perturbation analysis. Three cases of different groove configurations were chosen for the comparative study, e.g., the seal without rub groove (case1), the seal with upstream shifting of the seal tooth in respect to the rub groove (case2) and the seal with location of the seal tooth in the middle station of the rub groove (case3). The orifice contract coefficient adopted in reduction of rotordynamic coefficients was provided by using Computational Fluid Dynamics (CFD). Influence of rub grooves on the leakage flow was obtained in terms of the close-up view of the flow pattern near the seal tooth, leakage flow rate, distributions of the mean pressure and circumferential velocity in cavities. In comparison to case 1, the leakage flow in case 3 is considerably intensified, while which in case 2 is slightly increased. Dynamics and instability of the rotor in all cases was discussed in terms of the rotordynamic coefficients and the logarithmic decrement, respectively. The results disclosed that the aerodynamic forcing in case 2 intensified the destabilization of the rotor system.

Multi-layer Feed-forward Neural Network Deep Learning Control with Hybrid Position and Virtual-force Algorithm for Mobile Robot Obstacle Avoidance

Wei Zheng,Hong-Bin Wang,Zhi-Ming Zhang,Ning Li,Peng-Heng Yin 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.4

This paper addresses the trajectory tracking and obstacle avoidance control problems for a class ofmobile robot systems. Two classes of controllers are designed for the mobile robot system in the free motion,respectively. A new hybrid position virtual-force controller is designed to adjust the distance between the mobilerobot and the obstacles. Since the uncertainties between the mobile robot dynamics model and obstacles degradethe performance of the obstacle avoidance system, a multi-layer feed-forward neural networks (NNs) deep learningmethod with hybrid position and virtual-force is proposed, such that the distance between the mobile robot andthe obstacles converges to an adjustable bounded region. It is shown that the proposed controller in this paperis smooth, effective, and only uses the system output. The control design conditions are relaxed because of thedeveloped multi-layer feed-forward NNs deep learning compensator. The simulation results and obstacle avoidancecases are performed to show the effectiveness of the proposed method.