Clinical Significance of SH2B1 Adaptor Protein Expression in Non-small Cell Lung Cancer

Zhang, Hang,Duan, Chao-Jun,Chen, Wei,Wang, Shao-Qiang,Zhang, Sheng-Kang,Dong, Shuo,Cheng, Yuan-Da,Zhang, Chun-Fang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

The SH2B1 adaptor protein is recruited to multiple ligand-activated receptor tyrosine kinases that play important role in the physiologic and pathologic features of many cancers. The purpose of this study was to assess SH2B1 expression and to explore its contribution to the non-small cell lung cancer (NSCLC). Methods: SH2B1 expression in 114 primary NSCLC tissue specimens was analyzed by immunohistochemistry and correlated with clinicopathological parameters and patients' outcome. Additionally, 15 paired NSCLC background tissues, 5 NSCLC cell lines and a normal HBE cell line were evaluated for SH2B1 expression by RT-PCR and immunoblotting, immunofluorescence being applied for the cell lines. Results: SH2B1 was found to be overexpressed in NSCLC tissues and NSCLC cell lines. More importantly, high SH2B1 expression was significantly associated with tumor grade, tumor size, clinical stage, lymph node metastasis, and recurrence respectively. Survival analysis demonstrated that patients with high SH2B1 expression had both poorer disease-free survival and overall survival than other patients. Multivariate Cox regression analysis revealed that SH2B1 overexpression was an independent prognostic factor for patients with NSCLC. Conclusions: Our findings suggest that the SH2B1 protein may contribute to the malignant progression of NSCLC and could offer a novel prognostic indicator for patients with NSCLC.

Age-induced Changes in Ginsenoside Accumulation and Primary Metabolic Characteristics of Panax Ginseng in Transplantation Mode

Wei Yuan,Qing-feng Wang,Wen-han Pei,Si-yu Li,Tian-min Wang,Hui-peng Song,Dan Teng,Ting-guo Kang,Hui Zhang 고려인삼학회 2024 Journal of Ginseng Research Vol.48 No.1

Background: Ginseng (Panax ginseng Mayer) is an important natural medicine. However, a long culture period andchallenging quality control requirements limit its further use. Although artificial cultivation can yield a sustainablemedicinal supply, research on the association between the transplantation and chaining of metabolicnetworks, especially the regulation of ginsenoside biosynthetic pathways, is limited. Methods: Herein, we performed Liquid chromatography tandem mass spectrometry based metabolomic measurementsto evaluate ginsenoside accumulation and categorise differentially abundant metabolites (DAMs). Transcriptome measurements using an Illumina Platform were then conducted to probe the landscape of geneticalterations in ginseng at various ages in transplantation mode. Using pathway data and crosstalk DAMs obtainedby MapMan, we constructed a metabolic profile of transplantation Ginseng. Results: Accumulation of active ingredients was not obvious during the first 4 years (in the field), but followingtransplantation, the ginsenoside content increased significantly from 6 8 years (in the wild). Glycerolipidmetabolism and Glycerophospholipid metabolism were the most significant metabolic pathways, as Lipids andlipid-like molecule affected the yield of ginsenosides. Starch and sucrose were the most active metabolic pathwaysduring transplantation Ginseng growth. Conclusion: This study expands our understanding of metabolic network features and the accumulation of specificcompounds during different growth stages of this perennial herbaceous plant when growing in transplantationmode. The findings provide a basis for selecting the optimal transplanting time.

Nonsubsampled Contourlet Transform Based Infrared Image Super-Resolution by Using Sparse Dictionary and Residual Dictionary

Kangli Li,Wei Wu,Xiaomin Yang,Yingying Zhang,Binyu Yan,Wei Lu,Gwanggil Jeon 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.7

Due to the limitation of hardware, Infrared (IR) image has low-resolution (LR) and poor visual quality. To enhance the Infrared image’s resolution, super-resolution (SR) is a good solution. However, the conventional SR methods have some drawbacks. Firstly, the trained dictionary is an unstructured dictionary, which may lead to worse results. Secondly, the representation of the image is too simple to effectively represent image. Finally, only one high-resolution (HR)-LR dictionary pair is adopted to infer HR IR image. However one HR-LR dictionary pair is not good enough to obtain good results. To resolve these problems, in this paper, firstly, the sparse dictionary is introduced into the IR image SR to get better results. Secondly, nonsubsampled contourlet transform (NSCT) is employed to obtain a better representation of IR image. Finally, to achieve better r-esults, two HR–LR sparse dictionary pairs, which consists of a primitive sparse dictionary pair and a residual sparse dictionary pair, instead of one HR-LR dictionary pair are adopted. The experiment results indicate that the subjective visual effect and objective evaluation acquire excellent performance in the proposed method. Besides, this method is superior to other methods.

Etoposide-Cisplatin Alternating with Vinorelbine-Cisplatin Versus Etoposide-Cisplatin Alone in Patients with Extensive Disease Combined with Small Cell Lung Cancer

Zhang, Jie,Qi, Hui-Wei,Zheng, Hui,Chen, Mo,Zhu, Jun,Xie, Hui-Kang,Ni, Jian,Xu, Jian-Fang,Zhou, Cai-Cun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.10

Background: The aim of this study was to evaluate the efficacy of alternating etoposide-cisplatin and vinorelbine-cisplatin (EP-NP) compared with an etoposide-cisplatin (EP) regimen for advanced combined small cell carcinomas. Materials and Methods: Histologically confirmed combined small cell carcinoma patients who met the inclusion criteria were randomly assigned (1:1) into either the EP-NP setting (group A) or the EP setting (group B). The primary endpoint was progression-free survival in patients who received at least one dose of treatment. Results: Eighty-two patients entered into this trial, 42 in group A and 40 in group B. The objective response rates in group A and group B were 42.9% and 32.5%, respectively (p=0.334). Survival analysis showed that median progression-free survival was 6.1 months in group A, which was significantly longer than the 4.1 months in group B (p=0.041). However, as to overall survival, no significant difference was found between the two groups (11.0 vs 10.1 months in groups A and B, respectively, p=0.545). No unexpected side effects were observed in either group. Conclusions: The EP-NP regimen for combined small cell carcinomas prolonged progressio-nfree survival compared with the EP regimen. Further clinical investigations are warranted.

Ocular abnormalities in mice lacking the immunoglobulin superfamily member Cdo

Zhang, Wei,Mulieri, Philip J.,Gaio, Ursula,Bae, Gyu‐,Un,Krauss, Robert S.,Kang, Jong‐,Sun Blackwell Publishing Ltd 2009 FEBS JOURNAL Vol.276 No.20

<P>Vertebrate eye development requires a series of complex morphogenetic and inductive events to produce a lens vesicle centered within the bilayered optic cup and a posteriorly positioned optic stalk. Multiple congenital eye defects, including microphthalmia and coloboma, result from defects in early eye morphogenesis. Cdo is a multifunctional cell surface immunoglobulin superfamily member that interacts with and mediates signaling by cadherins and netrins to regulate myogenesis. In addition, Cdo plays an essential role in early forebrain development by functioning as coreceptor for sonic hedgehog. It is reported here that <I>Cdo</I> is expressed in a dynamic, but dorsally restricted, fashion during early eye development, and that mice lacking Cdo display multiple eye defects. Anomalies seen in <I>Cdo</I><SUP>−/<I>−</I></SUP> mice include coloboma (failure to close the optic fissure); failure to form a proper boundary between the retinal pigmented epithelium and optic stalk; defective lens formation, including failure to separate from the surface ectoderm; and microphthalmia. Consistent with this wide array of defects, developing eyes of <I>Cdo</I><SUP>−/<I>−</I></SUP> mice show altered expression of several regulators of dorsoventral eye patterning, including <I>Pax6</I>, <I>Pax2</I>, and <I>Tbx5</I>. Taken together, these findings show that Cdo is required for normal eye development and is required for normal expression of patterning genes in both the ventral and dorsal domains. The multiple eye development defects seen in <I>Cdo</I><SUP>−/<I>−</I></SUP> mice suggest that mutations in human Cdo could contribute to congenital eye anomalies, such as Jacobsen syndrome, which is frequently associated with ocular defects, including coloboma and Peters’ anomaly.</P>

Tracking of saltating sand trajectories over a flat surface embedded in an atmospheric boundary layer

Zhang, Wei,Kang, Jong-Hoon,Lee, Sang-Joon Elsevier 2007 Geomorphology Vol.86 No.3-4

<P><B>Abstract</B></P><P>Saltation is a major mechanism for the transport of soil particles. In the present study, we carried out wind tunnel tests to examine the saltating trajectories of two types of natural sand collected from a beach (diameter, <I>d</I>=300–500?μm and 200–300?μm respectively) as well as sand from the Taklimakan desert (<I>d</I>=100–125?μm) in an atmospheric boundary layer. Consecutive images of saltating particles were recorded using a high-speed digital camera at a rate of 2000?fps with a spatial resolution of 1024×1024?pixels. The high temporal resolution of the acquired images enabled us to study the particle motion very close to the surface. The saltating particle trajectories were reconstructed from consecutive images, and the physical quantities characterizing the initial and final stages of the particle flight in the windward direction at friction velocities of about 10%–25% above the threshold friction velocity (<I>u</I><SUB>⁎</SUB>/<I>u</I><SUB>⁎<I>t</I></SUB>=1.11–1.26) were analyzed statistically. In addition, the transverse deviation of the saltating particles from the main streamwise direction was evaluated. The results shed new light on the complicated motions involved in sand saltation and should prove useful in the evaluation and formulation of theoretical models.</P>

Fixed-time Fuzzy Adaptive Decentralized Control for High-order Nonlinear Large-scale Systems

Bo Kang,Zhiyao Ma,Yongming Li,Wei Zhang 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.12

This paper studies a fuzzy adaptive fixed-time tracking control issue for nonlinear high-order largescale systems. Fuzzy logic systems (FLSs) are utilized to identify unknown nonlinearities. Through using adaptive backstepping and adding a power integrator technique, the fixed-time decentralized control method is presented. It is proved that the tracking errors converge to a small neighborhood of a fixed time. A simulation example is presented to confirm the validity of the developed control method.

중국 전통가옥 객가(客家)의 공간 특징 및 선호도 인식 현황 분석 연구

장웨이(Zhang, Wei),강성곤(Kang, Sung-kon) 한국조형디자인학회 2021 조형디자인연구 Vol.24 No.4

본 연구는 중국 전통가옥 ‘객가(客家) 에 대하여 독특한 생활 문화적 특징과 건축문화의 지역성에 영향을 미치는 각 요소에 대하여 고찰을 통해 취락(聚落)형태, 공간배치, 재료사용 및 공간의 세부사항과 색채 등의 특징을 정리하고자 한다. 또한 전통가옥 객가문화(客家文化)의 공간 요소에 대한 선호도 인식 현황을 파악하고자 설문조사를 하여 이를 분석함으로써 앞으로 전통가옥 객가(客家)의 발전적 방향과 현대 건축에 활용될 수 있는 문화자원의 요소에 관한 이론적 근거를 제시하고자 한 것이다. 전통가옥 객가(客家)의 공간 구성의 특징을 살펴보면, 규칙적인 리듬감과 운율감 및 완결성, 그리고 이들 상호간의 관계에서 강한 휴머니즘적인 체계를 볼 수 있다. 특히 독창적인 엄격한 대칭적 구조와 조화로운 관계적 공간 구성은 인본주의적 감정의 중요성을 충분히 보여주고 있다. 또한 독특한 공간 형태와 배치는 중국 전통적인 객가문화의 역사적 맥락에서 표현되고 있을 뿐만 아니라 전통문화의 특성을 보여 주는 중요한 문화적 요소이며, 현대 공간 구성에 있어서도 새롭게 창의적으로 활용할 수 있다는 점에서 시사점이 높다고 하겠다. 본 연구의 논리적이고 객관적인 결과를 위하여, 문헌조사와 설문조사 방법을 활용하였으며, 중국 객가 전통가옥의 문화적 특징과 공간 특성에 대한 현대인들의 만족도 인식 현황을 조사하고 수집된 자료를 바탕으로 분석하고자 하였다. 설문조사의 결과로, 객가의 공간 구조의 특징들은 현대인들에게도 좋은 인지도와 만족도를 가지고 있으며, 향후 더 많은 사람들이 중국 전통가옥의 객가문화에 대하여 높은 인식을 가지고 사랑하게 될 것이라는 점도 이해할 수 있었다. 또한 본 결과는, 추후 중국 전통가옥 객가의 공간 형태 및 전통적 건축 재료와 현대적 활용 가능성, 전통적 색채의 현대적 변화 등의 측면에서 전통가옥 공간 구성에 대한 연구의 기초 자료와 토대를 마련하는데 중점을 두고자 하였다. 결론적으로, 본 연구를 통하여 중국 전통적인 객가문화(客家文化)의 정신을 이해하고 공간구성에 대한 대표적인 문화 요소들을 찾고, 또한 문화적 가치에 대한 높은 인식을 통해 객가문화에 대한 발전적 방향을 강구할 수 있도록 그 근거를 찾아 현대적인 새로운 의미를 부여하여야 한다는 점을 강조하고자 하며, 이를 바탕으로, 앞으로 중국 객가의 전통적 건축 공간 구성의 이념과 특징을 종합적으로 유추하여 중국의 현대적인 공간 특성에 대한 이론을 보완하고 새로운 개념으로 발전할 수 있기를 기대하고자 한다. The article starts from the study of the regional elements that influence the architectural culture of Hakka houses, and through the analysis of the various elements of the architectural beauty of Hakka houses, summarizes the characteristics of Hakka architecture in settlement form, spatial layout, use of materials, architectural details and colors, etc., and comprehensive analysis of preference recognition status through questionnaire survey is available. The reference to the spatial charateristics of new buildings today provides a corresponding theoretical basis for the creation of new Hakka traditional residential cultural buildings. Analyzing the preference recognition of space charateristics of the󰡐Hakka enclosure, we can discover its orderly sense of rhythm and rhythm; its integrity and primary and secondary relationship, showing a strong humanistic color; its strict symmetrical and harmonious relationship, fully demonstrating the humanity The important position of the complex in the space composition, if its unique shape and spatial layout are used as the inspiration of the creative source of modern space design, it can not only express the historical context of traditional Chinese Hakka culture, but also show the characteristics of traditional Chinese culture. This research attempts to use literature research and questionnaire survey methods to investigate the status quo of the spatial beauty of Chinese traditional Hakka folk house culture and analyze it based on the collected data. The survey results show that it can be confirmed that the beauty of space has a high degree of recognition and satisfaction in people s hearts, and it will attract more people to love the traditional Chinese Hakka folk house culture in the future. Resultinglly, this research seeks to find the most representative elements of traditional Chinese Hakka culture, analyzes the spirit of Hakka culture through space characteristics, gives Hakka culture a new look, and compares Hakka s construction concepts and characteristics of Hakka architecture with contemporary spatial structure theories. Hope It plays a role in the enrichment and development of contemporary architectural creation theory, and makes certain explorations and efforts for the perfection of contemporary characteristic of space theoretical framework.

Silencing of long noncoding RNA PVT1 inhibits podocyte damage and apoptosis in diabetic nephropathy by upregulating FOXA1

Dong-Wei Liu,Jia-Hui Zhang,Feng-Xun Liu,Xu-Tong Wang,Shao-Kang Pan,Deng-Ke Jiang,Zi-Hao Zhao,Zhang-Suo Liu 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

The number of patients with diabetic nephropathy (DN) is still on the rise worldwide, and this requires the development of new therapeutic strategies. Recent reports have highlighted genetic factors in the treatment of DN. Herein, we aimed to study the roles of long noncoding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) and histone 3 lysine 27 trimethylation (H3K27me3) in DN. A model of DN was established by inducing diabetes in mice with streptozotocin. Mouse podocyte clone 5 (MPC5) podocytes and primary podocytes were cultured in normal and high glucose media to observe cell morphology and to quantify PVT1 expression. The roles of PVT1 and enhancer of zeste homolog 2 (EZH2) were validated via loss-of-function and gain-of-function in vitro experiments to identify the interactions among PVT1, EZH2, and forkhead box A1 (FOXA1). The podocyte damage and apoptosis due to PVT1 and FOXA1 were verified with in vivo experiments. PVT1 was highly expressed in MPC5 and primary podocytes in DN patients and in cultures grown in high glucose medium. A large number of CpG (C-phosphate-G) island sites were predicted at the FOXA1 promoter region, where PVT1 recruited EZH2 to promote the recruitment of H3K27me3. The silencing of PVT1 or the overexpression of FOXA1 relieved the damage and inhibited the apoptosis of podocytes in DN, as was evidenced by the upregulated expression of synaptopodin and podocin, higher expression of Bcl-2, and lower expression of Bax and cleaved caspase-3. The key findings of this study collectively indicate that the suppression of lncRNA PVT1 exerts inhibitory effects on podocyte damage and apoptosis via FOXA1 in DN, which is of clinical significance.

Effect of Co Content on the Microstructure and Corrosion Behavior of Al0.3CoxCrFeNi High Entropy Alloys

Zequn Zhang,Qijuan Dong,Zhibin Chen,Junsheng Wu,Jiuyang Xia,Kang Huang,Pengcheng Zuo,Wei Xue,Bowei Zhang 대한금속·재료학회 2024 METALS AND MATERIALS International Vol.30 No.2

The influence of Co content on the microstructure and corrosion behavior of Al0.3CoxCrFeNi (x = 0.25, 0.5 and 1.0) highentropy alloys were investigated. The results indicated that the increased content of Co improve the corrosion resistanceof alloy, which can be attributed to the distinction of phase composition in alloys caused by different Co content. TheAl0.3CoxCrFeNi alloy shows a double-phase (FCC + BCC) structure for x = 0.25 and 0.5, and BCC phase consists of (Al,Ni)-rich B2 phase and (Cr, Fe, Co)-rich A2 phase. The increase of Co content induces the transformations of crystalstructuresfrom BCC to FCC, resulting in the decrease of BCC phase proportion. The Al0.3CoCrFeNi alloy exhibits thesingle FCC structure, and all elements tend to be homogeneously distributed. The difference in the potential of FCC andBCC phases indicates the BCC phase acts as anode resulting to localized corrosion, while B2 phase preferentially dissolvescompared to the A2 phase due to the lower potential. Therefore, the existence of selective corrosion is found in thealloys with double-phase, by contrast, the alloy with single FCC phase shows random pitting corrosion.