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Ultrastructure of Spermatogenesis in the Testis of Paragonimus heterotremus
Nongnut Uabundit,Pipatphong Kanla,Phongphithak Puthiwat,Channarong Arunyanart,Kowit Chaiciwamongkol,Wanchai Maleewong,Pewpan M. Intapan,Sitthichai Iamsaard,Wiphawi Hipkaeo 대한기생충학열대의학회 2013 The Korean Journal of Parasitology Vol.51 No.6
Pewpan M. Intapan,Oranuch Sanpool,Penchom Janwan,Porntip Laummaunwai,Nimit Morakote,Yoon Kong,Wanchai Maleewong 대한기생충학열대의학회 2013 The Korean Journal of Parasitology Vol.51 No.6
A synthetic peptide was prepared based on the antigenic region of Paragonimus westermani pre-procathepsin L, and its applicability for immunodiagnosis for human paragonimiasis (due to Paragonimus heterotremus) was tested using an ELISA to detect IgG4 antibodies in the sera of patients. Sera from other helminthiases, tuberculosis, and healthy volunteers were used as the references. This peptide-based assay system gave sensitivity, specificity, accuracy, and positive and negative predictive values of 100%, 94.6%, 96.2%, 100%, and 88.9%, respectively. Cross reactivity was frequently seen against the sera of fascioliasis (75%) and hookworm infections (50%). Since differential diagnosis between paragonimiasis and fascioliasis can be easily done by clinical presentation and fascioliasis serology, this cross reaction is not a serious problem. Sera from patients with other parasitoses (0-25%) rarely responded to this synthetic antigen. This synthetic peptide antigen seems to be useful for development of a standardized diagnostic system for paragonimiasis.