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Wanapat, M.,Kang, S.,Khejornsart, P.,Wanapat, S. Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.8
Four rumen-fistulated crossbred beef cattle (Brahman native) were randomly assigned according to a $4{\times}4$ Latin square design experiment to be fed plant herb supplements in their concentrate mixture. The treatments were: without herb supplementation (Control), lemongrass meal supplementation at 100 g/d (L), lemongrass meal supplementation at 100 g/d plus peppermint powder at 10 g/d (LP), and lemongrass meal supplementation at 100 g/d plus peppermint powder at 10 g/d with garlic powder 40 g/d (LPG), respectively. Based on the present study, the DMI and apparent digestibility of DM, OM, aNDF and ADF were not affected by dietary herb supplementation while CP digestibility tended to be decreased by herb supplement. Moreover, $NH_3$-N and BUN were decreased in all herb supplemented treatments and there was a tendency to an increase in ruminal pH in all herb supplemented groups. While there was no change in TVFA and C4 among lemongrass treatments, C2 was decreased in all herb supplemented treatments while C3 was increased. Methane production by calculation was the lowest in the LP and LPG groups. Population sizes of bacteria and protozoa were decreased in all herb supplemented groups, but not fungal zoospores. In all supplemented groups, total viable and proteolytic bacteria were decreased, while amylolytic and cellulolytic bacteria were similar. More importantly, in all herb supplemented groups, there were higher N balances, while there was no difference among treatments on purine derivative (PD) excretion or microbial N. Based on the results above, it could be concluded that there was no negative effect on ruminal fermentation characteristics and nutrient utilization by plant herb supplement, but protozoal population and $CH_4$ production were reduced. Thus, lemongrass alone or in combination with peppermint and garlic powder could be used as feed additives to improve rumen fermentation efficiency.
Wanapat, Metha,Promkot, C.,Wanapat, S. Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.7
Four male crossbred native beef cattle (average body weight of 427.7 kg) were randomly allocated to four types of cassoy-urea pellet as a source of protein in concentrate according to a $4{\times}4$ Latin square design to determine effect of diets on ruminal fermentation and nutrient digestibility. The four types of cassoy-urea pellets contained cassava hay, soybean meal, urea and binding agent at 79.2:19.8:0:1 (27.9% CP dry matter), 78.4:19.6:1:1 (30.4% CP), 77.6:19.4:2:1 (33.0% CP) and 99:0:0:1 (23.8% CP) for dietary treatments; 1, 2, 3 and 4, respectively. All four concentrate mixtures contained similar crude protein levels (11% CP) and were fed to animals in two equal parts (0.5% of body weight per day) while urea-treated rice straw (5% urea) was given ad libitum. The experiment revealed that dietary concentrate treatments had no effect on dry matter intake while digestibilities of neutral-detergent fiber and crude protein were higher (p<0.05) in cattle fed dietary treatments 1, 2 and 3 than in cattle fed dietary treatment 4. Ruminal ammonia-nitrogen ($NH_3$-N), was higher and acetic acid concentration (C2) and ratio of C2 to propionic acid (C3) were lower (p<0.05) in cattle fed dietary treatments 1, 2 and 3 than in those on treatment 4. It is concluded that use of cassoy-urea pellet as a protein source in concentrates for cattle resulted in improvement of digestibility, ruminal fermentation and rumen ecology. Further research using cassoy-urea pellet in feeding trials with milking cows and fattening beef should be undertaken.
Wanapat, M.,Pilajun, R.,Kang, S.,Setyaningsih, K.,Setyawan, A.R. Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.8
Four Thai - rumen fistulated male swamp buffaloes (Bubalus bubalis), about four years old with $400{\pm}20kg$ liveweight, were randomly assigned according to a $4{\times}4$ Latin square design to receive dietary treatments. The treatments were: ground corn cob (GCC) replacement for cassava chip (CC) in concentrate at 0% (T1); GCC replacement at 33% (T2); GCC replacement at 67% (T3); and GCC replacement at 100% (T4), respectively. During the experiment, concentrate was offered at 0.5% BW while 5% urea-treated rice straw was given at ad libitum. The result revealed that there was no effect of GCC replacement on DMI among treatments. In addition, digestibilities of DM, OM and CP were not different while aNDF linearly increased with an increasing level of GCC replacement. However, GCC replacement did not affect rumen fermentation such as ruminal pH, $NH_3$-N and VFA concentration; except C3 proportion which was the highest at 33% replacement while the lowest was at 100% replacement. All replacements of GCC resulted in similar protozoal and bacterial populations and microbial protein synthesis (MPS). Purine derivatives (PD) concentration in urine and PD to creatinine (PDC) index were varied with time of urination and among treatments at 0 to 8 and 8 to 16 h post feeding and higher values were shown among the GCC replacement groups. However at 16 to 24 h-post feeding, it was untraceable. In addition, creatinine concentration was similar among all treatments at every sampling time. Based on the above results, GCC can be used as an energy source for swamp buffalo fed with rice straw. Spot sampling of urine can be used for purine derivatives determination.
Wanapat, M.,Pimpa, O. Asian Australasian Association of Animal Productio 1999 Animal Bioscience Vol.12 No.6
The experiment was aimed at studying the effect of ruminal $NH_3-N$ levels on ruminal fermentation, microbial population, urinary purine derivative excretion, digestibility and rice straw intake in swamp buffaloes. Five, 3 to 4 years old, rumen fistulated swamp buffaloes were randomly assigned according to a $5{\times}5$ Latin square design to rceive five different intraruminal infusions of $NH_4HCO_3$ (0, 150, 300, 450 and 600 g/d) on a continuous daily basis. Rice straw as a roughage was offered ad libitum while concentrate was given at 0.8% BW daily. The results were that as levels of $NH_4HCO_3$ increased, ruminal $NH_3-N$ concentrations increased from 7.1 to 34.4 mg%. The highest digestibility and voluntary straw intakes were found at 13.6 to 17.6 mg% ruminal $NH_3-N$ levels; straw intake was highest at 13.6 mg%. Total bacterial and protozoal counts linearly increased as the ruminal $NH_3-N$ increased and were highest at 17.6 mg%. Total urinary purine derivatives and allantoin excretion were highest (44.0, 37.4 mM/d) at 17.6 mg% ruminal $NH_3-N$. Highest total VFAs (115 mM) were obtained a 13.6 mg% ruminal $NH_3-N$ while blood urea nitrogen significantly increased as ruminal $NH_3-N$ increased. The results from this experiment suggest that optimum ruminal $NH_3-N$ in swamp buffaloes is higher than 13.6 mg%, for improving rumen ecology, microbial protein synthesis, digestibility and straw intake.
Wanapat, M.,Petlum, A.,Pimpa, O. Asian Australasian Association of Animal Productio 1999 Animal Bioscience Vol.12 No.6
Twenty-four multiparous crossbred Friesian dairy cows (60-90 days in lactation) were randomly assigned into a $2{\times}2$ factorial arrangement in a randomized complete block design. Factors were two levels of concentrate supplementation (1:2, high vs 1:1.2, very high; concentrate:milk yield) and two levels of high-quality feed block (HQFB) supplementation (non vs ad libitum block licking). Ruzi grass (Brachiaria ruziziensis) was fed as a roughage throughout the 70 day feeding trial. High level of concentrate fed group resulted in higher roughage and HQFB intakes, compared with very high concentrate supplemented group. HQFB supplementation tended to increase roughage intake and significantly improved milk yield (2 kg/hd/d in high concentrate supplementation) and quality (% fat) which resulted in higher economical return. HQFB was recommended to be used as a strategic supplement in lactating dairy cows especially when fed on low-quality roughages or crop residues.
Wanapat, M.,Ngarmsang, A.,Korkhuntot, S.,Nontaso, N.,Wachirapakorn, C.,Beakes, G.,Rowlinson, P. Asian Australasian Association of Animal Productio 2000 Animal Bioscience Vol.13 No.7
A comparative study on rumen bacterial and protozoal population and fungal zoospores in cattle (Brahman$\times$Native) and swamp buffalo (Bubalus bubalis) was conducted. Forty animals, twenty of each, with same sex and similar age which were raised under similar condition in the Northeast of Thailand, were used. Rumen digesta were sampled immediately post slaughtering for total microscopic counts of bacteria, protozoa and fungal zoospores. It was found that total bacterial population were higher in swamp buffalo that those in cattle (1.6 vs $1.36{\times}10^{8}cells/ml$) having more population of cocci, rods and ovals. Lower rumen protozoal pupulation in swamp buffalo with lower numbers of Holotrichs and Entodiniomorphs were found as compared to those in cattle. Significant higher fungal zoospore counts were in swamp buffalo than those in cattle being 7.30 and $3.78{\times}10^6$, respectively. Study under electron microscope, revealed Anaeromyces sp. with acuminate apex were more predominant in the rumen of swamp buffalo. With these findings, cattle and swamp buffaloes showing differences in rumen bacterial, protozoal population and fungal zoospore counts, offer new additional information as why swamp buffaloes exhibit conditionally better than cattle especially during long dry season without green grass.
Wanapat, M.,Pimpa, O.,Petlum, A.,Wachirapakorn, C.,Yuanklang, C. Asian Australasian Association of Animal Productio 2000 Animal Bioscience Vol.13 No.6
A participation scheme involving smallholder dairy farmers in improving dairy productivity through the use of local feeds, on-farm established feeds and crop residues was carried out in the Northeast, Thailand. At six milk collection centers, 63 farmers with 340 lactating cows participated in this research and demonstration of feed supplements. Farmers and cows were allotted to receive respective feed supplements: high-quality feed block (HQFB), high-quality feed pellet (HQFP), dried cassava leaf/cassava hay, dried leucaena leaf and cottonseed meal: 5% urea treated rice straw was fed as a source of roughage throughout the feeding period of the dry season. Trainings and workshops were organized by the researchers at the University, research station, demonstration sites and on-farms. Regular visits to the fartns by researchers and extension officers were made while discussions and demonstrations were performed in addition. Participating farmers also visited other farmers during the demonstration which offered a real practical perspective and farmer-to-farmer interaction. As a result of this participation and demonstration scheme, the farmers could learn more effectively and accepted the technology more readily, especially the practicality of the feed preparation, feed establishment, feeding method and feed reserve. Strategic supplementation of these feed supplements resulted in improving milk yield, milk quality, overall condition of the cows and higher income return through increased productivity and lower level use of concentrate to milk yield from 1:2 to 1:3 or lower. Based on this research and demonstration /participation scheme, all feed supplements enhanced productivity, however the establishment of cassava hay on fartns deserved more attention and warrants a wider developmental expansion among dairy farmers since it contained high rumen by-pass protein (tannin-protein complex) and could be easily produced and be sustainable on farms.
Effect of Cassava Hay in High-quality Feed Block as Anthelmintics in Steers Grazing on Ruzi Grass
Wanapat, Metha,Khampa, S. Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.5
Six, one-year old dairy steers were randomly divided into two groups according to a Completely randomized design (CRD) to receive high-quality feed block (HQFB) without cassava hay and drenching (HQFB1+Ivomex) and HQFB with cassava hay (HQFB2) as block licks while grazing on Ruzi grass pasture. During the eight weeks, fecal parasitic egg counts dramatically declined for both treatment groups with 63.2 and 27.6% reduction from initial period for HQFB1+Ivomex and HQFB2, respectively. However, digestion of coefficients of nutrients particularly OM, were significantly higher in HQFB2 than, those in HQFB1+Ivomex, in addition, ADG of animals in HQFB2 tended to be higher than the group on HQFB1. It was, hence concluded that cassava hay could not only provide as a protein source but also serve as an anthelmintic in ruminants.
UTILIZATION OF ROUGHAGE AND CONCENTRATE BY FEEDLOT SWAMP BUFFALOES (BUBALUS BUBALlS)
Wanapat, M.,Wachirapakorn, C. Asian Australasian Association of Animal Productio 1990 Animal Bioscience Vol.3 No.3
Thirty-six yearling (18 bulls, 18 heifers) swamp buffaloes (Bubalus bubalis) with average liveweight $177{\pm}26kg$, were randomly allotted to receive 6 dietary treatments according to a $2{\times}3$ factorial arrangement in a completely randomized design (CRD). Factor A assigned for two types of straw; untreated rice straw (RS) and urea-treated (5%, w/w) rice straw (UTS). Factor B assigned for three ratio of roughage to concentrate (R:C) at 80:20, 50:50 and 20:80. Feeding trial lasted for 9 months during which respective feeds were offered at 3% body weight and adjusted at bi-weekly intervals using corresponding liveweights. Parameters measured under this experiment were feed intake, ruminal fluid for pH, $NH_3-N$, volatile fatty acids (VFA), liveweight change at bi-weekly intervals, carcass characteristics and cost-net profit analysis. It was found that intakes and digestion coefficients of DM, OM, CP except NDF and ADF were improved appreciably when ratio of concentrate increased. The average daily gain (ADG) and feed conversion ratio (FCR) were highest in group fed urea-treated rice straw at R:C levels of 20:80 (551.2 g/d, 10.7 kg/kg) and 50:50 (542.3 g/d, 10.6 kg/kg). It was obvious that FCR was best in the group fed on urea-treated rice straw (13.8 kg/kg) as compared to untreated rice straw fed-group (24 kg/kg). Carcass compositions of buffaloes measured resulted in 48.2 dressing percentage in all treatments offered at R:C levels of 50:50 and 20:80, however, loin eye area were 46.0, 53.6, 50.0 and $54.0cm^2$ for RS and UTS at respective levels of R:C. It was notable that carcass fat content was low which resulted in higher content of lean meat particularly in group fed UTS at 50:50 ratio R:C. Simple cost-net profit analysis was performed, it was found that net profits were obtained as follows 27, 30, -47, 44, 58, 22 $US/hd for respective treatment groups of RS and UTS at respective R:C levels. As shown, the best net profit resulted in group fed UTS at 50:50 R:C level.
Wanapat, M.,Varvikko, T.,Vanhatalo, A. Asian Australasian Association of Animal Productio 1990 Animal Bioscience Vol.3 No.2
An experiment was conducted with three ruminally and intestinally cannulated non-lactating cows of Finnish Ayrshire breed, to assess the ruminal degradation characteristics of oat (Avena sativa), rye (Secale cereale) and rice (Oryza sativa) straw by the nylon bag technique, and the subsequent post-ruminal degradation of their rumen-undegraded residues by using the mobile bag technique, respectively. The straw samples were untreated or treated with aqueous $NH_3$ or with urea solution in cold or hot water. The untreated straw samples were milled or chopped, and the treated straw samples were chopped. The constant values a, b, and c were computed according to the exponential equation, where a = intercept of degradation curve at time 0, b = potentially degradable material, c = rate of degradation of band (a+b) = maximum potential degradability (asymptote). It was found that nitrogen contents of chemically treated straw were markedly increased by both $NH_3$ and urea treatments. Milling the samples attributed to a remarkable loss at 0 h incubation time as compared to chopping of the respective samples. However, chemical treatment markedly improved the b value and the subsequent (a+b) values for dry matter, organic matter, neutral-detergent fiber, and acid-detergent fiber of the samples. Furthermore, temperature of the water used in the urea solutions was considered essential, since urea in hot water rather than in cold water seemed to enhance the overall degradability. The disappearance of rumen-incubated straw residues from the mobile bags ranged from 4.5 to 9.6% for the parameters measured. On average, the OM disappearance from bags was clearly higher for the residues of urea treated straw compared to those of ammonia treated straw, but the disappearance of NDF tended, however, to be higher on the ammonia treatment.