Metabolic Discrimination of Safflower Petals of Various Origins Using 1H NMR Spectroscopy and Multivariate Statistical Analysis

Wan Kyun Whang,Min-Won Lee,최형균 대한화학회 2007 Bulletin of the Korean Chemical Society Vol.28 No.4

The metabolic discrimination of safflowers from various geographical origins was performed using 1H nuclear magnetic resonance (NMR) spectroscopy followed by principal components analysis. With a combination of these techniques, safflower samples from different origins could be discriminated using the first two principal components (PC) of the 1H NMR spectra of the 50% methanol fractions. PC1 and PC2 accounted cumulatively for 91.3% of the variation in all variables. The major peaks in the 1H NMR spectra that contributed to the discrimination were assigned to fatty acid (terminal CH3), lactic acid, acetic acid, choline derivatives, glycine, and safflower yellow derivatives. In this study, we suggest that various types of safflower can be discriminated using PCA and 1H NMR spectra.

The Study of the origins of Curcuma Spp .

WHANG wan kyun,SHIN sang soo,KIM do hun,LEE sung wan,KIM sung kun,CHUNG sung hee 大韓藥學會 2006 大韓藥學會 總會 및 學術大會 Vol.2006 No.1

Metabolic Discrimination of Safflower Petals of Various Origins Using ¹H NMR Spectroscopy and Multivariate Statistical Analysis

Whang, Wan-Kyun,Lee, Min-Won,Choi, Hyung-Kyoon Korean Chemical Society 2007 Bulletin of the Korean Chemical Society Vol.28 No.4

The metabolic discrimination of safflowers from various geographical origins was performed using 1H nuclear magnetic resonance (NMR) spectroscopy followed by principal components analysis. With a combination of these techniques, safflower samples from different origins could be discriminated using the first two principal components (PC) of the 1H NMR spectra of the 50% methanol fractions. PC1 and PC2 accounted cumulatively for 91.3% of the variation in all variables. The major peaks in the 1H NMR spectra that contributed to the discrimination were assigned to fatty acid (terminal CH3), lactic acid, acetic acid, choline derivatives, glycine, and safflower yellow derivatives. In this study, we suggest that various types of safflower can be discriminated using PCA and 1H NMR spectra.

Flavonoids from Codonopsis lanceolata Leaves

Whang, Wan-Kyunn,Park, Kyun-Young,Chung, Sung-Hoon,Oh, In-Se,Kim, Il-Hyuk The Korean Society of Pharmacognosy 1994 생약학회지 Vol.25 No.3

For the investigation of resources in Codonopsis species, the studies were carried out to evaluate the pharmaco-constituents from the leaves of Codonopsis lanceolata (Campanulaceae) whose roots have been used to antitussive, expectorant, detoxicate, tonic, edible, etc. as a folk medicine in Korea. From the EtOAc and BuOH fractions of MeOH extract, three flavonoid compounds, $luteolin-7-O-{\beta}-_D-glucopyranoside$$(C_{21}H_{20}O_{1i},\;mp\;254{\sim}255^{\circ}$, compound 1), $luteolin-5-O-{\beta}-_D-glucopyranoside$$(C_{21}H_{20}O_{1i},\;mp\;279{\sim}281^{\circ}$, compound 2) and luteolin $(C_{15}H_{10}O_6,\;mp\;327{\sim}330^{\circ}$, compound 3) were isolated and identified on the basis of their physicochemical properties and spectroscopic evidences(UV, IR, $^1H-NMR$, $^{13}C-NMR$, MS etc.) in comparison with authentics respectively.

Studies on the Anti-Inflammatory Effects of Clerodendron trichotomum Thunberg Leaves

Choi, Jung-Ho,Whang, Wan-Kyun,Kim, Hong-Jin The Pharmaceutical Society of Korea 2004 Archives of Pharmacal Research Vol.27 No.2

Clerodendron trichotomum Thunberg Leaves (CTL) have been used for centuries in Chinese folk medicine for their anti-inflammatory properties. We have studied the anti-inflammatory effects of CTL extracts in rats, mice and in Raw 264.7 cells. 1 mg/kg solutions of the 30% and 60% methanol extracts of CTL were used and a 1 mg/kg of indomethacin was used as a positive anti-inflammatory standard; these were then administrated to rats. Carrageenan was injected subcutaneously to induce hind paw edema in rats. The result of carrageenan-induced rat paw oedema showed that a 1 mg/kg of the 30%, and 60% methanol fraction of CTL and 1 mg/kg of indomethacin inhibited the hind paw edema by 19.5%, 23.0%, and 20.5% respectively. The effect of CTL on inflammation in mice by a capillary permeability assay was examined by detecting Evans blue leakage from capillaries after the intraperitoneal injection of acetic acid, a potent inflammatory stimulus. The 60% methanol fraction of CTL inhibited Evans blue dye leakage by 47.0%, which was 10% higher than that of the inhibition of 1 mg/kg of indomethacin. Also, the 60% methanol fraction of CTL suppressed the prostaglandin $E_2$ ($PGE_2$) generation in RAW 264.7 macrophage cells after treatment with lipopolysaccharide (LPS) by as much as the inhibition of 1 mg/kg of indomethacin and this led to the synthesis of $PGE_2$ by COX-2 induction. The inhibition of the carrageenan-induced rat paw oedema, vascular permeability and the $PGE_2$ generation demonstrates that the 60% methanol fraction of CTL contains a potent anti-inflammatory activity.

Constituents of Crataegus Pinnatifida Var. psilosa Leaves (II) -Flavonoide from BuOH Fraction-

Oh, In-Se,Whang, Wan-Kyun,Kim, Il-Hyuk The Pharmaceutical Society of Korea 1994 Archives of Pharmacal Research Vol.17 No.5

The studies were camied out to evaluate the constituents in the leaves of Crataegus pinnatifida var. psilosa (Rosaceae) continuously. From the BuOH fraction of the MeOH extract, four flavonoid compounds, hyperoside (1), vitexin (2), 3"-O-rhamnosylvitexin (3) and $quercetin-3-O-{\alpha}-L-rhamnopyranoslyl-(1{\rightarrow}6)-{\beta}$-D-galactopyranoside (4) were isolated and identified on the basis of their physico-chemical properties and spectroscopic evidences by comparison with authentic samples.

수삼의 지역별 연근별 인삼사포닌 함량 비교

이충렬(Chung Ryul Lee),황완균(Wan Kyun Whang),신차균(Cha Gyun Shin),이학성(Hak Sung Lee),한성태(Sung Tai Han),임병욱(Byung Ok Im),고성권(Sung Kwon Ko) 한국식품과학회 2004 한국식품과학회지 Vol.36 No.5

흑삼의 인삼 사포닌 분석

한성태(Sung Tai Han),황완균(Wan Kyun Whang),김일혁(Il Hyuk Kim),양병욱(Byung Wook Yang),조순현(Soon Hyun Cho),고성권(Sung Kwon Ko) 대한약학회 2005 약학회지 Vol.49 No.6

The objective of this study is to provide the basic information for developing a high-value ginseng product using ginseng saponin and prosapogenin. In order to achieve such aim, Ginsenoside compositions of black ginseng (BG) extracts with various solvent conditions were examined by HPLC. The total saponin and the prosapogenin content of 95% ethyl alcohol extract were higher than that of the either 50% ethyl alcohol extract or distilled water extract. As a result, the order of the total saponin and the prosapogenin content was 1) 95% ethyl alcohol, 2) 50% ethyl alcohol, 3) the first and second mixture of 95% ethyl alcohol, distilled water, and 4) distilled water extract. In the case of fine black ginseng (FBG), the first and second mixture extracts of 95% ethyl alcohol and distilled water were the highest. In addition, the ratio of the protopanaxadiol group and the protopanaxatriol group (PD/PT) showed that the ratio of BG ranged from 0.304 to 0.601, while the ratio of FBG ranged from 1.166 to 1.657.

The Effect of Quercetin-3-O-β-D-Glucuronopyranoside on Indomethacin-induced Gastric Damage in Rats via Induction of Mucus Secretion and Down-regulation of ICAM-1 Expression

Xiang Mei Yan,Uy Dong Sohn,Min Jae Joo,Jae Chun Lim,Wan Kyun Whang,Sang Soo Sim,Chaeuk Im,Hak Rim Kim,Seok Yong Lee,In Kyeom Kim 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.9

The mechanism of the protective effect of quercetin-3-O-β-D-glucuronopyranoside (QGC) from the leaves of Rumex aqauticus on indomethacin (IND, a representative NSAID)-induced gastric damage in rats was investigated. Pre-treatment with QGC significantly attenuated INDinduced gastric mucosal injury. An increase in myeloperoxidase (MPO) activity and expression of intercellular adhesion molecule (ICAM)-1 protein and mRNA expression of the pro-inflammatory cytokines tumor necrosis factor-α and interleukin-1β, as well as a decrease in gastric mucus secretion were detected in the gastric mucosa of IND-treated rats. QGC reversed the side effect of IND on MPO activity and mucus production. Furthermore, QGC pre-treatment notably decreased ICAM-1 protein and mRNA expression of the pro-inflammatory cytokines, suggesting that QGC protection from IND-induced damage is associated with increased gastric mucus secretion, inhibition of free radical production by activated neutrophils via ICAM-1, and pro-inflammatory cytokine downregulation.

Acteoside와 Isoacteoside의 항산화 작용 및 미백 효과

윤미연,심상수,황완균,최병철,Yoon, Mi-Yun,Sim, Sang-Soo,Whang, Wan-Kyun,Choi, Byung-Chul 대한약학회 2009 약학회지 Vol.53 No.1

This study was performed to investigate the effects of acteoside and isoacteoside isolated from Clerodendron trichotomum Thunberg on melanin production in B16 melanoma cells. In DPPH radical scavenging activity, acteoside and isoacteoside had a potent anti-oxidant activity in a dose-dependent manner. Both acteoside and isoacteoside dose-dependently inhibited silica-induced ROS (reactive oxygen species) generation in B16 melanoma cells. They significantly inhibited tyrosinase activity and melanin production in MSH-stimulated B16 melanoma cells. The inhibitory effect of acteoside was more potent than that of isoacteosidee. In Western blot of tyrosinase, acteoside inhibited MSH-induced tyrosinase expression in B16 melanoma cells, which is related to the inhibitory action of acteoside on tyrosinase activity and melanin production. These results show that acteoside and isoacteoside from Clerodendron trichotomum Thunberg has a potent antioxidant activity and whitening activity. The underlying mechanism of acteoside on whitening activity may be due to the inhibition of tyrosinase activity and tyrosinase expression.