상업적 응유효소의 탈지유에 대한 단백질 분해 작용

신현수,김상범,임종우 한국동물자원과학회 2002 한국축산학회지 Vol.44 No.6

상업적 단백질 분해 효소에 0.02% CaCl_2를 첨가하여 응유 활성화를 시킨 탈지유에 대한 분해 작용의 결과를 요약하면 다음과 같다. 다양한 효소별 가수분해 시간에 따른 가수분해도는 미생물 유래 효소와 trypsin은 pepsin과 papain W-40보다 높은 분해도를 나타냈다. 12% TCA 용액에 가용성인 NPN의 양은 trypsin이 가장 높은 분해도를 나타내었고 rennet과 pepsin이 가장 낮은 분해도를 보였다. 전기영동에 있어서 trypsin과 protease S는 α-lactalbumin을 분해하였고 papain w-40은 β-lactoglobulin을 미약하게 분해하였으며 neutrase 1.5는 90분 이후부터 α-lactalbumin과 β-lactoglobulin을 분해하였다. Rennet과 비교한 전기영동상에서는 rennet에 의해 분해 되지 않은 α_s-casein과 β-casein을 trypsin과 protease S가 다량 분해하였고 k-casein은 rennet에 비해 papain W-40이 상당 수준의 분해상을 나타내었다. 이상의 결과 가수분해도 및 NPN 양은 trypsin, neutrase 1.5 및 protease S가 다른 효소에 비해 높게 나타났으며, 전기영동상에서는 pepsin과 neutrase 1.5가 rennet과 유사한 경향을 나타내었다. Proteolytic activities of some commercial milk clotting enzymes(rennet, trypsin, pepsin, papain W-40, neutrase 1.5 and protease S) in bovine skim milk containing 0.02% CaCl_2 were determined by measuring DH(Degree of Hydrolysis), NPN(Non Protein Nitrogen) and by comparing patterns of SDS-PAGE(Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis). The DH of microbial enzymes(neutrase 1.5 and protease S) and trypsin in bovine skim milk were higher than those of pepsin and papain W-40. The amounts of NPN in the milk treated with trypsin and the other animal enzymes(rennet and pepsin) showed the highest and lowest degrees of proteolysis, respectively. SDS-PAGE showed that trypsin and protease S hydrolyzed α-lactalbumin and papain W-40 hydrolyzed β-lactoglobulin slightly, while neutrase 1.5 hydrolyzed both α-lactalbumin and β-lactoglobulin after treating for 90 min. Trypsin and protease S easily hydrolyzed α_s-casein and β-casein, which were not hydrolyzed by rennet. Papain W-40 hydrolyzed k-casein more than rennet as shown in SDS-PAGE. Based on the results of the experiments, the DH and NPN of trypsin, neutrase 1.5 and protease S were shown to be higher than those of the other enzymes. The SDS-PAGE patterns of papain W-40 and neutrase 1.5 were similar with that of rennet.

산란계 개량에 관한 연구 1 . 순계의 생산능력 비교시험

상병찬,강만석,김동곤,한성욱 ( B . C . Sang,M . S . Kang,D . K . Kim,S . W . Han ) 한국축산학회 1980 한국축산학회지 Vol.22 No.5

This experiment was carried out to test performance of the domentic purebred fowl. The purpose of this experiment was to compare the productive performance of the purebred fowl to obtained basic materials for laying performance improvement by means of cross breeding. In this study, 1, 200 chickens was used-that is 6 purebred strains White Leghorns(720 chickens), 1 purebred strain Rhode Island Reds (120 chickens), 1 purebred strain New Hampshires (120 chickens). The results obtained in this study are as follows; 1. The average Viability of brooding, rearing and laying period was best for Rhode Island Reds and E. Strain among White Leghorns. 2. The average body weight at 8 weeks, first egg production days, 500days of age in W. L. was 546.86g. 1431.37g, 1738.34g, respectively, but there was no significant difference among. purebred strains of W. L. 3. The average Sexual maturity of 50% egg production was 170.58 days in W. L., but R. I. R. and S. N. H. were 189.94 days, 182.42days, respectively. There was significant difference at 1 % level among Strains. 4. The average hen-housed egg production was 214.16 eggs in W. L., but R. I. R. and S. N. H. were 214.22 eggs, 196.74 eggs, respectively. There was significant difference at 1% level among Strains, but the highest hen-housed egg production (231.84 eggs) was E. Strain among strains. 5. The average egg weight was 54. 48g in W. L, but R. I. R. and .S. N. H. were 56.12g, 54.90g, respectively, The highest egg weight (57.69g) was Coronial Strain of W. L. among strains. There was significant difference at 1 % Level among strains. 6. The average feed requirement was 3.01 in W. L. but R. I. R. and S. N. H. were 3.23, 3.52, respectively the highest feed requirement (2.79) was Coronial Strain of W. L. among strains. There was significant difference at 1 % level among strains. 7. On the basis of the results of this study, the productive performance was best for C. D. E. strains among W. L. and R. I. R.

<i>S100A9</i> and <i>EGFR</i> gene signatures predict disease progression in muscle invasive bladder cancer patients after chemotherapy

Kim, W. T.,Kim, J.,Yan, C.,Jeong, P.,Choi, S. Y.,Lee, O. J.,Chae, Y. B.,Yun, S. J.,Lee, S. C.,Kim, W. J. Oxford University Press 2014 Annals of Oncology Vol.25 No.5

<P>In our previous gene expression profile analysis, IL1B, S100A8, S100A9, and EGFR were shown to be important mediators of muscle invasive bladder cancer (MIBC) progression. The aim of the present study was to investigate the ability of these gene signatures to predict disease progression after chemotherapy in patients with locally recurrent or metastatic MIBC. Patients with locally advanced MIBC who received chemotherapy were enrolled. The expression signatures of four genes were measured and carried out further functional analysis to confirm our findings. Two of the four genes, S100A9 and EGFR, were determined to significantly influence disease progression (P = 0.023, 0.045, respectively). Based on a receiver operating characteristic curve, a cut-off value for disease progression was determined. Patients with the good-prognostic signature group had a significantly longer time to progression and cancer-specific survival time than those with the poor-prognostic signature group (P < 0.001, 0.042, respectively). In the multivariate Cox regression analysis, gene signature was the only factor that significantly influenced disease progression [hazard ratio: 4.726, confidence interval: 1.623-13.763, P = 0.004]. In immunohistochemical analysis, S100A9 and EGFR positivity were associated with disease progression after chemotherapy. Protein expression of S100A9/EGFR showed modest correlation with gene expression of S100A9/EGFR (r = 0.395, P = 0.014 and r = 0.453, P = 0.004). Our functional analysis provided the evidence demonstrating that expression of S100A9 and EGFR closely associated chemoresistance, and that inhibition of S100A9 and EGFR may sensitize bladder tumor cells to the cisplatin-based chemotherapy. The S100A9/EGFR level is a novel prognostic marker to predict the chemoresponsiveness of patients with locally recurrent or metastatic MIBC.</P>

한우 포유모우의 산유량및 유조성분에 관한 연구 1 . 포유모우의 산유량 추정방법 확립에 관한 연구

강수원(S . W . Kang),정연후(Y . H . Chung),정창화(C . H . Chung),나승환(S . H . Na),손용석(Y . S . Son),백동훈(D . H . Baik) 한국축산학회 1989 한국축산학회지 Vol.31 No.3

This experiment was conducted to establish the estimating of dam`s milk producing capacity which forms the basis of Korean Native Cattle improvement for 3 years from 1982 to 1984. Four methods, including weight difference of the calf before and after nursing, hand milking and machine milking were compared with a new milking while the calf nurses, which the good points from the existing methods were extracted and then were developed. Fifty nine heads of nursing cow were tested to investigate dam`s milk yield. dam`s milk composition by the new milking method for 3 years from 1985 to 1987. The results of this experiment are summarized as follows: 1. This new method was made to use a portable vacuum·operated bucket milker in two quarters of each cow`s left or right udder while the calf suckles two quarters of his or her dam`s right or left udder. On this method. cows and calves were seperated at 8:00 am and at 5:00 pm the calves allowed to nurse. Cows and calves remained seperated over night. At 8:00 am and 5:00 pm the following day, the calves allowed to nurse one udder half while the rest was milked by machine. 2. On the comparison with methods estimating dam`s milk yield, the method by calf-weight difference before and after nursing wasn`t exact and good method in estimating dam`s milk yield and composition because not only this method was indirectly estimated by calf`s weight gain but also milk sample could not be taken. And also, the method by hand milking and machine milking could be surveyed only 60 days and 4 to 10 days, respectively, because dam`s milk yield was rapidly decreased with passing the milking day. However, by the new method, milking could be completely done without failure for 180 days and samples for analyzing the milk composition could be taken more easily and exactly than any other method. 3. Daily average and total milk yield of fifty nine heads investigated by this new method for 180 days was 3.48 and 625.8kg, respectively. 4. In the milk composition, total solids, far, protein, lactose and ash content was 14.21, 4.64, 4.07, 4.21 and 0.89 percent, respectively.

Salmonella Typhi, Salmonella Typhimurium 및 Salmonella Enteritidis의 항균제 감수성 (1997)

신영학,유정식,김기상,정동준,오경수,이점규,이상원,이근영,박미선,이복권,김호훈 국립보건연구원 1998 국립보건원보 Vol.35 No.-

Role of the Meckel’s Cartilage in Embryonic Mandibular Development of Mice

J. W Choi,S. B Han,J. H Sung,H. I Shin 대한구강악안면병리학회 2005 대한구강악안면병리학회지 Vol.29 No.5

Mecke!'s car t ilage is one of the ea rliest structu re to appear in a mandible derived from the lï rst branchi a l a rch and serves as the primorclium I"or the formation 01‘ mandible‘ mall eus. incus. and sphenomandibular li gament However. its direct role a nd the mechanism in mandibular clevelopment a re not well elucidated. 1'0 address t his Issue‘ we observed morphol ogical and histological changes and gene expression patterns in the Mecke!'s cart ilage 01" a cleveloping mouse. I"rom E13.5 to E18.5 embryos. using skeletal preparation samples a ncl routinely prepa red s lide secti ons for light mi croscopic observation in various sectional planes. The following methods were per |‘ ormecl : H&E staining I"or general hi st이 og i cal observation ‘ Von Kossafor detection of minerali zation. TRAP activ ity staining for locali zaLion 01’ osteoclastic cell s. immunohistochemistry for !Iα@-1 a ncl -9 forevaluati on of enzy matic activity 01" osteoclasLic cell s. a ncl in situ hybricli zation for detection of collagen type 1. Il. ancl X mRNA ex presslon‘ respecLively. AL E1 3.5 Mec kel's cartilage appeared as a V-shaped rod fused a t the micl line and thin minera li zed ma ndibular buccal plaLe was I"ormed lateral to. and at some clistance from. Meckel’s carti lage in an intramembranous ossi lïcation mocle. WiLh the progression of tooth development. t he Meckel’s in carti lage adjacent incisors revealecl hyperLrophi c chonclrocyte di ff"er entiation with minerali zation of the chondroid matrix. The Meckel’s car Li lage was replacecl with bone by o~ L eoc l asLs . showing strong immunoreact ivity for MMP- l ancl -9 from E16 5 Wi Lh ti me‘ Lhis bony replacement of Meckel's cartilage in an endochondral ossification mode was ex Lenclecl up Lo the mid-porLion of Lhe molar sockets til l EI8.5. The bony replacement of minera li zed hypertrophic chondrocyte zone expressing X collagen mHNA conLri buted to the formation of thick mandibular lingual plate . 1'hese f"i ndings suggesL LhaL mandibular formalion and development is closely relatecl with not only Mecke!'s carLi lage. buL also wiLh Lhc developing LooLh. and thaL C'erLai n in f"l uence from the developing tooth may play a role in detcrmin in g Lhe faLc of Meckel’s ca rLi lage cluring ma ndi bular development.

단세포군 항체를 이용한 Shigella serotyping serum 개발(Ⅱ) : S. flexneri type Ⅰ,Ⅱ & type Ⅰ:6, group 6

정의범,이영희,김순남,박강수,이명숙,성원근,김종욱,홍승의,박정우 국립보건연구원 1994 국립보건원보 Vol.31 No.1

기계체조선수(器械體操選手)의 총지방량(總脂肪量)에 관(關)한 연구(硏究)

白元俊(W.J.Paik),金尙民(S.M.Kim),兪承熙(S.H.Yoo) 한국체육학회 1975 한국체육학회지 Vol.11 No.-

We computed the % Fat by the measured values of skinfold thickness at 4 sites; back, waist, abdomen, arm with 20 male gymnasic players(8 Kyung Hee high school, 12 Kyung ung Hee university players) and with 16 Female gymnastic player (8 Kyung He girl’s high school players, 8 Kyung Hee university players) hat all (total) 36 players were executed.Calculation Formula● high school players% Fat=1.324×Mean Skinfold thickness(mm) -0.045● Male College players% Fat=0.911×Mean Skinfold thickness(㎜)+8.1● Female high school and college players% Fat=0.58×Mean skinfold thickness(㎜)+14.311. The player’s average Skinfold thickness of 4 sites was less that of non-athletic persons.2. Both of male and Female gymnastic players showed the maximum value on the among 4 sites, while non-athltic males showed in back and Female in Arms, the maximum value.3. The Female player’s Skinfold thickness was thicker than that of male about 5.8㎜.4. The Kyung Hee high school gymnastic players skinfold thickness is as follws;back 6.9㎜waist 8.3㎜abdomen 5.9㎜arm 4.8㎜, the skinfold thickness averaged 6.3㎜and the Kyung Hee university gymnastic player’sSkinfold thickness is as follows;back 6.5㎜waist 8.9㎜abdomen 5.8㎜arm 4.7㎜, the Skinfold thickness averaged 6.4㎜5. The Kyung Hee girl’s High school player’s skinfold thickness is as follows;back 9.6㎜waist 12.1㎜abdomen 11.9㎜arm 8.3㎜, the Skinfold thickness averaged 10.1㎜ and the women’s skinfold thikness(Kyung Hee university gymnastic players) is as follows;back 10.8㎜waist 16.7㎜abdomen 11.0㎜arm 11.3㎜, the skinfold thickness averaged 12.4㎜6. The male players % Fat 11.7% (High school players 8.3%, college players 14.0%) and Female player’s % Fat was 2.8% (girl’s High school player’s 20.1%, colleye players 21.5%)7. Both of male and Female gymnastic player’s % Fat was less that of non-athletic persons and college player’s % Fat was more than that of high school players.

O/W · W/O Emulsion의 Tribology 특성 연구

이봉구,임웅빈,오성모 원광대학교 생산 및 자동화기술연구소 1995 생산.자동화기술연구소논문집 : 원광대 Vol.- No.2

We have been so much interested in the lubrication of emulsion property recently. In particular, O/W and W/O type emulsion have a good property to be used in the Lubricative situation of several services. Therefore, in this section we have studied about the lubricational characteristics in Neat Oil, O/W and W/O type emulsion using the Falex, wear test machine. The result is as follows. 1. In the case of O/W and W/O type emulsion, the beginning friction coefficient than Neat Oil, but it becomes more excellent as the time passes. especially, the W/O type becomes the more excellent. 2. As the load increase, a change in temperature shows to us be nearly similar and all of them, the O/W type is the most excellent in the temperature characteristic. 3. In the beginning, the anti-wear characteristic of emulsion lowers, but after the some load has stability, O/W and W/O type is excellent in order of anti-wear characteristic. 4. In the regular hours, a change in the emulsion becomes settled, so the character of temperature is superior. specially, O/W type is predominant. Therefore, the emulsion in case of the O/W and W/O type are more excellent than Neat oil in the thermal stability and can improve the characteristics of tribology.

早期破膜에 關한 臨床的 考察

許湘,吳文愛,安東源,申吳永 대한산부인과학회 1972 Obstetrics & Gynecology Science Vol.15 No.7