조사료의 종류가 홀스타인 송아지 유선의 발달 , 반추위 돌기 및 성장률에 미치는 영향

조광근,최윤재,박영우,윤연화,한영근,윤상기,권웅기 한국영양사료학회 1999 韓國營養飼料學會誌 Vol.23 No.6

본 연구는 홀스타인 송아지에 대한 세 종류의 조사료가 유선조직, 반추위 유두, 번식성적 및 성장률에 미치는 영향을 조사하기 위하여 실시하였다. 출생 후 4일된 송아지(42.9±0.9㎏) 24두를 무작위로 선발하여 출생 후 4∼44일까지 전유를 급여하다가 45일에 그룹 1은 볏짚, 그룹 2는 오차드 그래스, 그룹 3은 알괄파를 급여하였다. 또한 유선조직과 반추위의 발달을 측정하기 위하여 3, 9, 18개월령에 도달하였을 때 각 처리구의 송아지를 1마리씩 도살하였다. 알팔파를 급여한 그룹 3은 다른 처리구에 비하여 중체율이 높았으며, 그룹 2는 영양소 이용률과 번식성적이 높은 경향을 나타내었다. 반추위의 무게는 3개월령 도달시에는 그룹 3이 가장 무거웠으나, 9개월과 18개월령 도달시에는 그룹 2에서 가장 무거운 것으로 나타났다. 반추위의 유두 수는 유두의 성장과 함께 일반적으로 감소하는 경향을 나타내어 유두의 길이와 유두 수와는 서로 상대적인 관계를 나타내었다. 유선의 기능적 활성을 나타내는 RNA/DNA 함량은 3개월 시에는 그룹 3이 가장 낮은 경향을 보였으나 9, 18개월 시에는 반대로 가장 높은 경향으로 나타났으며, 유선세포의 크기를 나타내는 protein/DNA 함량은 18개월 시에 그룹 2가 가장 높은 경향을 보였다. 결론적으로 본 연구에서 볏짚은 반추위의 초기 발달을 촉진하고 알팔파와 오차드그래스는 성장과 번식성적을 증가시키며 또한 초기에 유선의 발달을 촉진시키는 것으로 나타났다. Twenty-four Holstein calves were used to evaluate the effects of three different forages on growth and reproductive performances, and ruminal papillary and mammary developments. Four-day-old Holstein calves weighing 42.9±0.9 ㎏ were randomly assigned to three forage groups of rice straw (group 1), orchard grass hay (group 2), and alfalfa (group 3). All calves received whole milk from day 4 through day 44. At 45 days of age, the animals were assigned at random to one of the three dietary treatments. When calves reached the ages of 3, 9, and 18 months, one calf in each group was sacrifced for evaluation of ruminal and mammary tissue developments. Calves of group 3 gained more body weight than those of other groups, while group 2 showed an effective nutrient utilization and reproduction performance. At 3 months, the stomach weight of group 3 was the highest, however, group 2 provided the highest stomach weight at 9 and 18 months. A remarkable reduction in papillae number was observed in the rumens of all 3- to 9-month-old heifers. A decrease in number of these papillae was observed in all groups exhibiting papillary growth. There was a reciprocal relationship between the number and the length of ruminal papillae. Among the 3-month-old calves, the functional activity (RNAI DNA contents) of the mammary glands of group 3 was lower than those of other groups, whereas the trend was opposite for 9- and 18-month-old heifers. The size of mammary cell (protein / DNA contents) was the greatest for group 2 at 18 months of age. The results suggest that rice straw stimulated earlier development of rumen. Alfalfa and orchard grass improved the growth, reproduction performance, and earlier development of mammary glands.

운동후 측복통과 함께 발생한 급성 신부전 1 예

김종순,박영주,조종태,이정임,신민호,구천희,박태용,한승수,강진화 대한내과학회 1996 대한내과학회지 Vol.50 No.6

We report a case of acute renal failure with severe loin pain and patchy renal vasoconstriction after exercise. An immediate postcontrast computed tomogram of this case shows multiple patchy areas of poor contrast enhancement in the cortex of the both kidneys. A 24-hours delayed scan without further injection of contrast dye shows multiple wedge-shaped contrast enhancement in the coincident areas of poor enhancement in the immediate scan. The 2 and 5 days delayed scans show that wedge-shaped contrast enhancement becomes disappeared gradually to the renal cortex, and the 8 days delayed scan shows complete disappearance of contrast enhancement. The renal scintigrams with technetium-99m-methylene diphosphonate and dimercaptosuccinic acid show diffuse increased uptakes and multiple patchy photon deficient areas in the both kidneys respectively. The follow-up renal scintigrams show nearly normal findings, This patient had an uneventful hospital course and recovered completely without dialysis.

급성 골수성 백혈병(M6)에 합병된 중추성 요붕증 1 예

김성연,조보연,이홍규,고창순,민헌기,김병국,김노경,신찬수,이명식,강덕현,조종태 대한내과학회 1989 대한내과학회지 Vol.37 No.2

Leukemia is known to be an extremely rare cause of diabetes insipidus. We report a case of diabetes insipidus as a complication of leukemia. An 18 year-old man was admitted because of polyuria polydipsia and fever. Bone marrow aspiration and biopsy revealed the diagnosis of acute erythroleukemia (AML, M6). On the water deprivation test, the diabetes insipidus was confirmed to be of the central type. He was managed with intranasal vasopressin for diabetes insipidus with good response. Induction chemotherapy was administered without response, and the patient died of septicemia and respiratory failure 2 months after initial diagnosis. A short review of the literature related to this subject is also presented.

한국에서의 Human T-Cell Leukemia Virus Type-1(HTLV-1)에 대한 혈청학적 및 역학에 관한 연구

이영열,이성열,김은실,이홍복,김병국,이문호,박선양,김노경,서철원,규경채,최강원,조한익,고월청,야마구찌이찌세이 대한내과학회 1986 대한내과학회지 Vol.31 No.2

Type-C retrovirus, human T-cell leukemia virus type-l(HTLV-1), has been shown to be associated etiologically with adult T-cell leukemia, which is an endemic disease in southwestern parts of Japan. We examined the seroepidemiological characteristics of antibody to adult T-cell leukemia virus associated antigen (anti-ATLA-antibody) by the indirect immunofluorescent assay and ELISA from the healthy individuals and various diseased patients in the Republic of Korea, a country adjacent to Japan in which HTLV-1 is endemic. The results were as follows. 24 out of 9,603 individuals(0.25%) were seropiositive. The positve rate was 0.17% in males and 0.36% in females, the sex difference was female predominent relatively. HTLV-1 carriers were found 16 individuals in Seoul, 5 in Kyonggi area, 1 in Chungnam and Jonbuk, respectively, but found only 1 individual in Busan city and Jaejoo area close to southwestern parts of Japan. Positive rate to anti-ATLA-antibody seemed to be high in older age, especially at the 50s(0. 4Yo). In conclusion the prevalence rate of anti-ATLA- antibody seemed to be very low in the Republic of Korea adjacent to Japan in which HTLV-1 is endemic and no patient with adult T-cell leukemia has been found as yet. Also we noted that the Republic of Korea is not an endemic area to HTLV-1.

사료의 pH 및 단백질 수준이 자돈의 성장 및 영양소 소화율에 미치는 영향

김지훈,한인규,김진동,조원탁,현충남,이지훈 한국영양사료학회 1999 韓國營養飼料學會誌 Vol.23 No.4

자돈사료내 pH와 단백질 수준의 효과를 알아보기 위하여 2개의 시험을 수행하였다. 시험 1에서는 pH 수준이 자돈의 성장과 영양소 소화율에 미치는 영향을 총 150두의 자돈(L×D×LW ; 8.82㎏ BW 30 ± 2일령)을 이용하여 1) pH 4.7, 2) pH 5.2, 3) pH 5.7 (basal diet), 4) pH 6.2, 5) pH 6.7의 다섯처리를 두었고, 시험 2에서는 pH와 단백질 수준의 효과를 알아보기 위하여 150두의 자돈을 (L×D×LW ; 5.46㎏ BW) 2×3 요인법으로 배치하였다. (조단백질 = 21.5, 24.5%, 사료 pH = 4.5, 5.5, 6.5). 또한 소화율의 측정을 위하여 시험 1에는 25두, 시험 2에는 24두의 자돈에 T-cannular를 설치하였다. 시험 1에서 pH는 자돈의 성장에 아무런 영향이 없는 것으로 나타났다. 낮은 pH사료를 급이한 구에서 사료효율이 약간 좋은 경향이 있었으나, 유의적인 차이는 발견되지 않았다. 회장 및 분소화율도 처리에 의한 효과가 발견되지 않았다. 시험 2에서는 낮은 pH 사료를 급이한 구에서 성장 및 사료효율이 개선되는 경향이 있었다 (P = 0.16). 사료효율은 단백질 수준에 의한 영향이 있었으며 (P<0.05), pH와 단백질 수준간의 상호작용이나 다른 효과는 발견되지 않았다. 회장 소화율도 단백질 수준에 의한 영향은 있었으나, pH의 효과는 methionine의 소화율에만 영향이 있는 것으로 나타났다. 전체적으로 소화율은 pH에 의한 일정한 경향이 발견되지 않았다. 본 시험의 결과는 사료의 pH 조절효과는 자돈의 나이나 사료의 종류에 따라 영향을 받는다는 것을 암시하며, 일반적으로 통용되고 있는 자돈사료는 특별한 산성화 처리가 필요하지 않은 것으로 나타났다. Two experiments were conducted to investigate the effect of diet pH and protein levels on the growth performance and nutrients digestibility in young piglets. In Exp. 1, a total of 150 piglets (Landrace × Duroc × Large White ; 8.82㎏ B W, 30 ± 2 days of age) were allotted into 5 treatments. Treatments were 1) pH 4.7, 2) pH 5.2, 3) pH 5.7 (basal diet), 4) pH 6.2 and 5) pH 6.7. In Exp. 2, the effect of dietary protein and diet pH levels on the growth performance and nutrient digestibility was examined with 150 crossbreds Landrace × Duroc × Large White ; 5.46㎏ BW) piglets in 2 × 3 factorial arrangement. (21.5 and 24.5% CP and diet pH (4.5, 5.5 and 6.5)). Each treatment has 5 replications, 5 heads per replicate. For the digestibility determination, 25 pigs for Exp 1. and 24 pigs for Exp. 2. were fitted with simple T-cannula and individually fed the experimental diet. In Exp. 1, no significant difference was found in any of the criteria measured. Pigs fed low pH diets showed slightly better feed efficiency, but the difference was not significant. Both ileal and fecal nutrient digestibility were not different. A small difference was found in major amino acids digestibility, but there was no constant trend detected. In Exp. 2, pigs fed low pH diet showed a trend (P = 0.16) to grow faster than those fed high pH diet. Feed efficiency was affected by protein level (P<0.05). No other main effect was detected. Protein level affected the ileal digestibility of energy, dry matter, crude fat, methionine and threonine. The effect of pH was found only in methionine digestibility. No other main effect was found in ileal digestibility. It appeared that age of pigs and type of diet could greatly influence the effect of diet pH manipulation. Acidification of commercially available diets might have minimal effect when diet contained high portion of high quality feed ingredients.

방전 플라스마에 의한 CFC-12(CCl2F2)의 분해

황명환,우인성,조정국,강현춘,안형환,이한섭,강안수 한국산업안전학회 1999 한국안전학회지 Vol.14 No.3

Decomposition efficiency, power consumption, and applied voltage of CFC(Chlorofluorocarbon) were investigated by SPCY(surface induced discharge plasma chemical processing) reactor to obtain optimum process variables and maximum decomposition efficiencies. Decomposition efficiency of CFC-12 with various electric frequencies(5∼50㎑). flow rates (100∼1,000mL/min), initial concentrations(100∼1,000ppm), electrode materials(W, Cu. Al), electrode thickness(1, 2, 3mm) and reference gases(N₂, O₂, air) were measured and the products were analyzed with FT-IR. Experimental results showed that at the frequency of 10kHz, the highest decomposition efficiency of 92.7% for CFC-12 were observed at the power consumptions of 29.6W, respectively, and that decomposition efficiency decreased with increasing frequency above 20㎑ and decomposition efficiency per unit power were 3.13%/W for CFC-12. Decomposition efficiency was increased with increasing residence times and with decreasing initial concentration of pollutants. Decomposition efficiency was increased with increasing thickness of discharge electrode and the highest decomposition efficiency was obtained for the electrode diameter of 3mm. As the electrode material, decomposition efficiency was in order that tungsten(W), copper(Cu), aluminum (Al).

급성췌장염에 의한 췌장가성남종에서 내시경적 배액술후 발생한 췌장가성남종과 십이지장 및 대장에 발생한 내부 췌장루 1예

김연수,이문성,김진오,조영덕,이영홍,봉형근,조주영,이준성,심찬섭,황성규,전기원,변재호,장재영 대한소화기학회 1996 대한소화기학회지 Vol.28 No.1

MANNOPROTEIN 과 GLUCAN 의 첨가가 쥐의 성장과 NATURAL KILLER CELL ACTIVITY 에 미치는 영향

손광수,조광근,최윤재,윤연화,김재영,한영근,문태현,김성찬 한국영양사료학회 1999 韓國營養飼料學會誌 Vol.23 No.6

본 연구의 목적은 효모 세포벽, 또는 효모의 세포벽으로부터 본 연구에서 확립한 방법으로 분리한 mannoprotein(MP)과 glucan(Glu)을 동물에 급여하므로써 동물의 일당중체량, 일당사료섭취량과 사료:중체량의 비율, 그리고 동물이 가지고 있는 면역기능의 활성화정도를 조사하는데 있다. 산업적 측면에서 대량 추출하기 위하여 기존에 있는 방법을 개선하여 변형된 방법으로 효모세포벽제제를 분리하였다. 본 연구에서 분리된 효모세포벽제제(cell wall, mannoprotein glucan)와 시판중인 효모세포벽제제(Bio-Mos^(TM))를 0.1%로 4주령 횐쥐에 4주간 급여하였다. 일당중체량은 개선된 방법으로 분리한 glucan을 급여한 처리구에서 가장 높은 중체량을 나타냈으며, 다음으로 mannoprotein 급여구가 높았으며 cell wall과 시제품(Bio-Mos^(TM)) 급여구에서 낮은 중체량을 보였다. 일당사료섭취량에 있어서 glucan 급여구가 cell wall과 시제품 급여구들과 비교할 때 높았으며 사료:중체량 비율 또한 glucan 급여구에서 가장 좋은 경향을 보였다. 사양 4주깨 횐쥐로부터 spleen을 채취하여 YACl 세포에 대한 natural killer cell의 cytotoxicity를 분석한 결과 본 실험실에서 분리한 mannoprotein과 glucan이 면역 증진효과가 큰것으로 보였다. Mannoprotein 급여구와 glucan 급여구에서 대조구와 cell wall 급여구보다 면역 효과가 뚜렷하게 나타났으며(MP, P<0.01; Glu, P<0.05), mannoprotein 급여구가 glucan 급여구보다 natural killer cell의 활성도가 높았다(P<0.01). 결론적으로 효모세포벽, mannoprotein과 glucan을 횐쥐에 급여했을 때 성장능력에 있어서 유의성 있는 효과가 나타나지 않았지만 세포배양수준에서 spleen natural killer cell의 activity를 증가시키는 효과가 있다. The purpose of this study was to investigate the average daily gam, average daily feed intake, feed gain ratio and immune system activation of rats when feeding yeast cell wall, mannoprotein (MP) and glucan (Glu) which were isolated from yeast cell wall. In order to isolate the yeast cell wall products in large scale, we used the improved isolation method basing on the typical one. In this study, yeast cell wall products (cell wall, mannoprotein, glucan) and commercial product (Bio-Mos^(TM)) were fed to 4-week old rats by 0.l% during 4 weeks experimental period. Glucan(extracted from improved method) provided the highest average daily gain. Mannoprotein treated group showed the second highest average daily gain. Cell wall and Bia-Mos^(TM) fed groups showed lower average daily gain. Treatment fed glucan also showed higher average daily feed intake than that of cell wall and commercial Bio-Mos^(TM) groups. And in feed: gain ratio, glucan fed rats showed the respectively good results. At the 4th week of feeding trial, rats' spleens were obtained from all the treatments to evaluate the cytotoxicity of natural killer cell against YACl tumor cell. The results indicated that mannoprotein and glucan had the significant effects on activating immune system. Rats of mannoprotein and glucan treated groups showed the higher immune activity than that of cell wall and control groups (MP, P<0.01; Glu, P<0.05), and natural killer cell activity of mannoprotein fed rats was higher than that of glucan fed rats (P<0.01). In conclusion, although the addition of yeast cell wall, mannoprotein and glucan to the diet of rats did not provide the significant effects on growth performance, it was clear that the mannoprotein and glucan isolated in this study could stimulate the activity of spleen natural killer cell in the cell culture.

성인에서 발견된 십이지장 막양구조

박경규,박형근,김연수,이문성,김진오,조영덕,이영홍,봉형근,조주영,이준성,심찬섭,황성규,함정식,장재영,권귀향 대한내과학회 1997 대한내과학회지 Vol.53 No.2

Duodenal obstruction is the third leading cause of intrinsic obstruction of the alimentary tract in the newborn, the other causes are imperforate anus and esophageal atresia. If the obstruction is incomplete, as with a fenestrated duodenal membrane, the presentation of the symptoms may be delayed and the diagnosis overlooked. And so they are rarely diagnosed in the adult. Diagnosis can be made by contrast duodenography but is more definitive with endoscopy. This is the first case report of a 21-year-old female with duodenal membrane, associated with incomplete rotation of intestine and uterus bicornis, diagnosed by hypotonic duodenography and endoscopy, preoperatively, and successfully treated with duodenoduodenostomy.

14 일령 이유자돈을 위한 적정 LACTOSE : SUCROSE 비율 SUCROSE RATIO FOR THE PIGS WEANED AT 14 DAYS OF AGE

한인규,김진동,조원탁,한영근,이찬호,이기웅,박향숙 한국영양사료학회 1999 韓國營養飼料學會誌 Vol.23 No.6

본 실험은 lactose에 대한 sucrose 대체가 14일령 조기 이유자돈의 성장에 미치는 영향을 알아보기 위하여 수행하였다. lactose와 sucrose의 적정비율을 결정하기 위해 평균체중 5.02±0.18 ㎏인 14일령 이유자돈 80두를 4처리 4반복, 반복당 5두씩 완전임의로 배치 하였으며, 처리구별 lactose와 sucrose의 비율은 각각 100:0(처리구 A), 75:25 (처리구 B), 50:50 (처리구 C), 25:75 (처리구 D) 및 0:100 (처리구 E)이었다. 이유초기 7일동안 뿐 아니라 시험 전 기간에 걸친 자돈의 일당중체량 및 일당사료섭취량은 처리 평균간 통계적인 유의차를 나타내지 않았으며, 다만 시험 전기간에 걸친 사료요구율에 있어서만 유의차가 인정되었으며, B와 D 처리구가 특히 우수한 것으로 나타났다 (p<0.05). 이유 1주동안 (Phase I)의 영양소 소화율은 DM과 CP를 제외하고는 처리 평균간 유의차가 인정되지 않았으며, A 처리구의 CP소화율은 다른 처리구에 비해 유의적으로 높았다 (P<0.05).그러나 B, C, D 그리고 E 처리구간에는 유의적인 차이가 보이지 않았다. 이유 14∼21일령 (Phase II)의 영양소 소화율은 처리평균간 유의차가 인정되지 않았으며, 이유 3주간에 걸친 건물, 질소 및 인의 배출량 역시 처리 평균간 유의차를 나타내지 않았다. 본 실험결과에서 나타난 바와 같이 14일령 이유자돈에 대한 탄수화물 공급원으로서의 lactose에 대한 sucrose의 대체는 자돈의 생산성 감소 및 영양소의 배출량 증가와 같은 불리함이 없이 이루어질 수 있음을 의미한다. This experiment was conducted to determine the optimum ratio of lactose to sucrose for nursery pigs weaned at 14 days of age. A total of 80 weaned pigs (BW 5.02±0.18 ㎏) were allotted into one of five treatments using a completely randomized block design. Treatments were 100:0 (A), 75:25 (B), 50:50 (C), 25:75 (D) and 0 :100 (E) in lactose : sucrose ratio. For the first week after weaning, no significant differences were found in average daily gain (ADG), average daily feed intake (ADFI) and feed conversion ratio (FCR, feed/gain). There were also no significant differences in ADG, ADFI and FCR among treatments during the second and third week after weaning. During overall experimental period, FCR was significantly influenced by dietary treatment, especially B and D treatments showed better FCR than other treatments (p<0.05). During the initial 7 day postweaning (Phase I), the nutrient digestibility was not influenced by the lactose: sucrose ratio except for dry matter (DM) and crude protein (CP). CP digestibility of diet A was significantly higher than those of other diets (p<0.05), but no significant.differences were found among diets B, C, D and E. During the second and third week postweaning (phase II), no significant differences were found in digestibilities of GE, DM, CA and P among treatments. There were no significant differences in nutrients excretion among treatments. These results indicate that sucrose can be well utilized by baby pigs as the source of carbohydrate after 15 days of age. It implies that lactose added in weaner diets could be partially replaced with sucrose.