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Koli, Valmiki B.,Kim, Jung-Sik Elsevier 2019 Materials science in semiconductor processing Vol.94 No.-
<P><B>Abstract</B></P> <P>Simple sol-gel method has used to synthesize TiO<SUB>2</SUB>-CeO<SUB>2</SUB> nanocomposites. XRD analysis carried out to determine the phase and structural properties of the synthesized nanocomposites. Thermal and gravimetric stability of the samples were determined by TGA. The optical spectroscopic study revealed that the nanocomposites show the shifting of band edge towards higher wavelength with an increase in the CeO<SUB>2</SUB> content in nanocomposites as compared to pure TiO<SUB>2</SUB>. Morphology of the synthesized nanocomposites was confirmed by FE-SEM and TEM analysis. Synthesized nanomaterials are in spherical with particle sizes in the range 10–20 nm. The HR-TEM image showed the formation of the heterojunction between CeO<SUB>2</SUB> and TiO<SUB>2</SUB> NPs and their elemental composition was confirmed by EDS. Surface chemical bonding states and the electronic valence band positions of the elements in nanocomposites were determined by XPS analysis. Photoluminescence study revealed the effect of CeO<SUB>2</SUB> concentration on photogenerated electron-hole pairs in TiO<SUB>2</SUB> nanoparticles. Photocatalytic activity of synthesized nanomaterials was studied by decomposition of toluene gas with irradiation of UV light. Revealed that nanocomposites with TiO<SUB>2</SUB>:CeO<SUB>2</SUB> ratio of 3:1 had the highest photocatalytic efficiency as compared to the other nanocomposites and pure TiO<SUB>2</SUB> and CeO<SUB>2</SUB> nanoparticles. Therefore, the results confirmed that the coupling of TiO<SUB>2</SUB> with CeO<SUB>2</SUB> absorb the photon energy and generates reactive oxygen species, which are easily transferred to CeO<SUB>2</SUB>, causing enhances the separation of the electron-hole pair and an improved the photocatalytic activity of the nanocomposites.</P>
Rajesh, Ramanna Valmiki,Kim, Seong-Kon,Park, Mi-Rim,Nam, Jin-Seon,Kim, Nam-Kuk,Kwon, Seulemina,Yoon, Du-Hak,Kim, Tae-Hun,Lee, Hyun-Jeong Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.1
Anatomically separate fat depots differ in size, function, and contribution to pathological states such as the metabolic syndrome. We isolated pre-adipocytes from different adipose depots, omental, subcutaneous and intramuscular, of beef cattle, and cultured in vitro to determine the basis for the variations and attribute these variations to the inherent properties of adipocyte progenitors. The proliferating cells from all depots before the confluence were harvested and the proteome was analyzed by a functional proteomic approach, involving 2-DE and MALDI-TOF/TOF. More than 252 protein spots were identified, selected and analyzed by Image Master (ver 7.0) and MALDI-TOF/TOF. Further, our analysis showed that there were specific differences in proteome expression patterns among proliferating precursor cells from the three depots. Sixteen proteins were found to be differentially expressed and these were identified as proteins involved in cellular processes, heat shock/chaperones, redox proteins, cytoskeletal proteins and metabolic enzymes. The results also enabled us to understand the basic roles of these proteins in different inherent properties exhibited by adipose tissue depots.
Proteomic Analysis of Bovine Muscle Satellite Cells during Myogenic Differentiation
Rajesh, Ramanna Valmiki,Jang, Eun-Jeong,Choi, In-Ho,Heo, Kang-Nyeong,Yoon, Du-Hak,Kim, Tae-Hun,Lee, Hyun-Jeong Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.9
The aim of this study was to analyze the proteome expression of bovine satellite cells from longissimus dorsi (LD), deep pectoral (DP) and semitendinosus (ST) muscle depots during in vitro myogenic differentiation. Proteomic profiling by twodimensional gel electrophoresis and mass spectrometry of differentiating satellite cells revealed a total of 38 proteins that were differentially regulated among the three depots. Among differentially regulated proteins, metabolic proteins like lactate dehydrogenase (LDH), malate dehydrogenase (MDH) were found to be up regulated in ST, while alpha-enolase (NNE) in LD and DP depot satellite cells were down regulated. Also, our analysis found that there was a prominent up regulation of cytoskeletal proteins like actin, actincapping protein and transgelin along with chaperone proteins like heat shock protein beta 1 (HSPB 1) and T-complex protein 1 (TCP-1). Among other up regulated proteins, LIM domain containing protein, annexin 2 and Rho GDP-dissociation inhibitor 1 (Rho GDI) are observed, which were already proven to be involved in the myogeneis. More interestingly, satellite cells from ST depot were found to have a higher myotube formation rate than the cells from the other two depots. Taken together, our results demonstrated that, proteins involved in glucose metabolism, cytoskeletal modeling and protein folding plays a key role in the myogenic differentiation of bovine satellite cells.
Proteomic Analysis of Bovine Longissimus Muscle Satellite Cells during Adipogenic Differentiation
Rajesh, Ramanna Valmiki,Park, Mi-Rim,Heo, Kang-Nyeong,Yoon, Du-Hak,Kim, Tae-Hun,Lee, Hyun-Jeong Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.5
Satellite cells are skeletal muscle progenitor/stem cells that reside between the basal lamina and plasma membranes of skeletal fibers in vivo. These cells can give rise to both myogenic and adipogenic cells. Given the possible role for differentiation of satellite cells into adipocytes in marbling and in some pathological disorders like sarcopenia, knowledge of the proteins involved in such process remains obscure. Using two-dimensional polyacrylamide gel electrophoresis coupled with mass spectrometry, we investigated the proteins that are differentially expressed during adipogenic differentiation of satellite cells from bovine longissimus muscle. Our proteome mapping strategy to identify the differentially expressed intracellular proteins during adipogenic differentiation revealed a total of 25 different proteins. The proteins up-regulated during adipogenic differentiation of satellite cells like Cathepsin H precursor, Retinal dehydrogenase 1, Enoyl-CoA hydratase, Ubiquinol-cytochrome-c reductase, T-complex protein 1 subunit beta and ATP synthase D chain were found to be associated with lipid metabolism. The down-regulated proteins like LIM protein, annexin proteins, cofilin-1, Rho GDP-dissociation inhibitor 1 and septin-2, identified in the present study were found to be associated with myogenesis. These results clearly demonstrate that the adipogenic conversion of muscle satellite cells is associated with the up-regulated and down-regulated proteins involved in adipogenesis and myogenesis respectively.
Transcriptional Profiling of Differentially Expressed Genes in Porcine Satellite Cell
Jin Young Jeong,Jang Mi Kim,Ramanna Valmiki Rajesh,Sekar Suresh,Gul Won Jang,Kyung-Tai Lee,Tae Hun Kim,Mina Park,Hak Jae Jeong,Kyung Woon Kim,Yong Min Cho,Hyun-Jeong Lee 한국동물번식학회 2013 Reproductive & developmental biology Vol.37 No.4