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Umami Nafiatul,Rahayu Eka Rizky Vury,Suhartanto Bambang,Agus Ali,Suryanto Edi,Rahman Mohammad Mijanur 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.1
Objective: One hundred hybrid male ducks (Mojosari×Alabio) were used to examine the efficacy of chicory supplementation as nutritional feed manipulation on production performance, and blood lipid profile of hybrid ducks. Methods: The ducks were tagged, weighed, and then allotted randomly to one of the four treatment diets using a completely randomized design. The experimental diets were: i) P0 (100% basal diets+0% chicory as control), ii) P1 (95% basal diets+5% chicory), iii) P2 (90% basal diets+10% chicory) and iv) P3 (85% basal diets+15% chicory). For each treatment group, there were 5 replicates of 5 birds each. All experimental diets were isonitrogenous and isocaloric using locally available ingredients. Results: Hybrid ducks with fed diets supplemented fresh chicory (5%, 10%, and 15%) showed increased feed intake and body weight gain, as well as feed conversion ratio to be smaller than those ducks fed diets without chicory supplementation (control). The ducks fed 10% chicory supplementation contained significantly (p<0.05) lower ash and higher organic matter contents of meat than those ducks fed other diets. The ducks fed 15% chicory supplementation showed the lowest crude protein and cholesterol content of meat among the treatment diets. Ducks fed chicory supplementation showed lower (p<0.05) blood cholesterol and triglyceride levels than those ducks fed without chicory supplementation, while dietary interventions had no major (p>0.05) influence on low-density lipoprotein and high-density lipoprotein levels in duck blood. Conclusion: In this study, 10% chicory supplementation showed the best results characterized by an increase in growth performance, carcass quality, small intestinal histomorphology, and lower cholesterol levels of meat.
Kusumawati Arizah,Mustopa Apon Zaenal,Umami Rifqiyah Nur,Santoso Adi,Wibawan I Wayan Teguh,Setiyono Agus,Sudarwanto Mirnawati Bachrum 한국미생물·생명공학회 2022 한국미생물·생명공학회지 Vol.50 No.3
Aptamers are short, chemically synthesized, single-stranded DNA or RNA oligonucleotides that fold into unique three-dimensional structures. In this study, we aim to determine the antibiofilm activity and binding specificity of the six polyclonal DNA aptamers (S15K3, S15K4, S15K6, S15K13, S15K15, and S15K20) on Staphylococcus aureus BPA-12 and Escherichia coli EPEC 4. Aptamer S15K6 showed the highest percentage of antibiofilm activity against S. aureus BPA-12 (37.4%) as shown by the lowest OD570 value of 0.313. Aptamer S15K20 showed the highest percentage of antibiofilm activity against E. coli EPEC 4 (15.4%) as shown by the lowest OD570 value of 0.515. Aptamers S15K13 and S15K20 showed antibiofilm activities against both S. aureus BPA-12 and E. coli EPEC4, and thus potentially have broad reactivity. Furthermore, based on the binding capacity and Kd values from our previous study, the binding specificity assay of selected polyclonal DNA aptamers (S15K3 and S15K15) against S. aureus BPA-12, E. coli EPEC 4, S. aureus BPA-6, S. agalactiae, E. coli MHA-6, and Listeria monocytogenes were performed using qPCR. Aptamers S15K3 and S15K15 showed specific binding to S. aureus BPA-12, E. coli EPEC 4, S. aureus BPA-6, and S. agalactiae, but could not bind to E. coli MHA-6 and L. monocytogenes. Therefore, this study showed that the polyclonal DNA aptamers have antibiofilm activity and were able to bind to S. aureus BPA-12 and E. coli EPEC 4 bacteria.
Dian Astuti,Bambang Suhartanto,Nafiatul Umami,Agung Irawan 한국작물학회 2020 Journal of crop science and biotechnology Vol.23 No.3
This field study was conducted to investigate the effect of spaced-row pattern and NPK fertilizer rates on productivity and quality of inter cropped sorghum and Stylosanthes guianensis. Three spaced-row pattern (S1 = 90x20 cm, S2 = 75x25 cm, and S3 = 60x30 cm, respectively) and NPK fertilizer application rates (N1 = 0 without NPK, N2 = 100 kg/ha, N3 = 200 kg/ha, respectively) were investigated under two local sorghum varieties (V1 = var. Kawali and V2 = var. Super-2) in a 2x3x3 split-split-plot design. Intercropped sorghum x stylosanthes in S3 resulted in higher dry matter (DM) content and biomass production than that in S1. NPK fertilization significantly increased DM and organic matter (OM) production by 16.2–115.0% and 16.1–115.2%, respectively, compared to those without NPK fertilization. Interaction effects of V x NPK and V x S x NPK were detected that contributing to the increase in biomass and nutrient quality of the forages. In vitro DM and OM digestibility rates for V1-S were higher than at V2-S. In conclusion, V2 produced a higher biomass, whereas V1 was superior in terms of nutrient quality. Spaced-row at 60x30 cm and 100 kg/ha of NPK were efficient in improving the productivity and nutrient quality of sorghum and stylosanthes.
Recent advancements of nitric oxide-releasing hydrogels for wound dressing applications
Hasan Nurhasni,Jiafu Cao,Mustopa Apon Zaenal,Himawan Achmad,Umami Rifqiyah Nur,Ullah Muneeb,Wathoni Nasrul,유진욱 한국약제학회 2023 Journal of Pharmaceutical Investigation Vol.53 No.6
Background Hydrogels are three-dimensional (3D) polymer networks that can absorb significant volumes of water inside their interstices and continue connecting these interstices while maintaining an inflated network structure; this capability is due to their unusual 3D crosslinked polymer meshwork structure. Hydrogels are promising wound dressings, particularly for chronic wounds, owing to their wound-healing properties, such as flexibility, adhesion, moisture control, biocompatibility, and biodegradability. Nitric oxide (NO) is a small molecule that has been thoroughly investigated for its wound-healing activity. The diverse roles of NO in wound healing require its stable delivery to the wound site. Thus, hydrogels have been evaluated as promising scaffolds for storing and releasing NO in a controlled manner to promote and accelerate wound healing. Area covered This review sought to introduce the types of polymers used to prepare hydrogel-based wound dressings and the types of NO donors used as wound healing promoters. The preparation method of the hydrogels and their physical and chemical properties were presented herein, and recent studies on NO-releasing hydrogels for wound therapy were summarized and discussed. Selected hydrogels with unique characteristics and significant findings for wound healing were also emphasized. Expert opinion Owing to the importance of chronic wounds in healthcare, the development of functional materials that support proper and rapid wound healing is required. NO-releasing hydrogels can be employed for wound dressing applications owing to their controlled NO releasability and antibacterial and wound healing activities.