Comparison of Casein, Soy Protein and Tenebrio molitor Larva Fermentation Extract as a Dietary Protein Source in Type 2 Diabetic (db/db) Mice

Asli Turkyilmaz,Ju Ri Ham,Ra-Yeong Choi,Jisu Han,Soo-Young Choi,Hae-In Lee,Mi-Kyung Lee 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

Tenebrio molitor larva, known as mealworm, has various bioactivities such as anti-obesity, anticancer, and antioxidant. We previously showed that fermented Tenebrio molitor larva (TMP) contains higher amino acid than that of non-fermented and its anti-hepatic steatosis activity in obese mice. This study compared TMP to casein (CA) and soy protein (SP) as a dietary protein source in type 2 diabetic mice. Each db/db mouse group was fed a AIN-76 diet containing 20% CA, SP, and TMP for 6weeks. TMP significantly lowered serum AST and ALT, while it increased serum HDL-cholesterol level compared to the CA and SP groups. Serum free fatty acid levels in SP and TMP groups were lower than in the CA group. Food intake in the TMP group was higher than in CA and SP, but the body weight did not differ among the groups. Fasting blood glucose level was the lowest in CA group. TMP showed a tendency to decrease serum leptin levels of db/db mice. TMP reduced hepatic lipid droplets and triglyceride content compared to the CA and SP groups. Taken together, TMP exhibits a beneficial effect on hepatosteatosis and HTR in type 2 diabetic mice, suggesting a potential as a protein source.

Effects of Exogenous Salicylic Acid on Antioxidant Activity and Proline Accumulation in Apple (Malus domestica L.)

Bengu Turkyilmaz Unal,Oguzhan Mentis,Ethem Akyol 한국원예학회 2015 Horticulture, Environment, and Biotechnology Vol.56 No.5

This study was conducted to determine the effects of exogenous application of different levels of salicylic acid (SA; 0 mM, 3.62 mM, and 7.24 mM) on antioxidant activity and proline accumulation in apple (Malus domestica Borkh cv. Red Chief Delicious) trees during late spring frost. The study was performed in Ulukısla, Nigde, Turkey from December 2012 to June 2013. We measured the levels of photosynthetic pigments, total proteins and proline in leaves, as well as superoxide dismutase and peroxidase enzymatic activities. We also performed morphological observations of the trees. The study was planned according to random experimental design. We determined that SA application increased the fruit number, shoot number, and carotenoid contents in the leaves, but this increase was not statistically significant. However, the fruit weights, superoxide dismutase and peroxidase activities, as well as chlorophyll, protein, and proline levels increased significantly in response to SA treatment compared to the control. In addition, the treated fruits were darker than the control. These results suggest that treating apple trees with exogenous SA may increase antioxidant enzyme activities as well as protein and proline levels and may alleviate the effects of late spring frost.

Fermented Extracts of Tenebrio molitors larvae Replacement for Casein Alters Hepatic Proteomic Profiles in Type 2 Diabetic Mice

Asli Turkyilmaz,Hae-In Lee,Mi-Kyung Lee 한국식품영양과학회 2022 한국식품영양과학회 학술대회발표집 Vol.2022 No.10

Erratum - Influence of various metal oxides on mechanical and physical properties of heat-cured polymethyl methacrylate denture base resins

Ilser Turkyilmaz,Turan Korkmaz,Hai Albayrak,Neset Volkan Asar 대한치과보철학회 2013 The Journal of Advanced Prosthodontics Vol.5 No.4

Simultaneous adsorption of selected VOCs in the gas environment by low-cost adsorbent from Ricinus communis

Isinkaralar Kaan,Turkyilmaz Aydin 한국탄소학회 2022 Carbon Letters Vol.32 No.7

Benzene, toluene, ethylbenzene, and xylenes are commonly known as (BTEX) and include volatile organic compounds (VOCs) in ambient air. Exposure to some BTEX has been associated with health risks. This study aimed to reduce BTEX on the environment and human health dramatically. This research targeted decreasing the BTEX in an air environment by producing high surface area activated carbon (KA-AC) under optimized synthesis conditions from Ricinus communis as lignocellulosic waste using ZnCl2 solution, respectively. The influence of several activation parameters was investigated on the surface area, such as impregnation ratio, carbonization time, and carbonization temperature. The KA5-AC prepared under optimized conditions showed BET surface area and total pore volume of 1225 m2/g, and 0.72 cm3/g, respectively. The optimized synthesis conditions were as follows: 0.1, 0.5, 1, 2, and 5 M impregnation ratio, 450–950 °C carbonization temperature, and 100 min carbonization time. The characteristics of the optimized KA-AC were analyzed using nitrogen adsorption–desorption isotherm, scanning electron microscopy, and pore structural analysis. The results confirmed that the VOCs adsorption on KA-AC followed a monolayer adsorption isotherm over a homogeneous adsorbent surface. It showed the removal efficiency of benzene, toluene, ethylbenzene, and m, p-xylene (R2 = from 0.991 to 0.997). Moreover, the KA-AC exhibited good performance without considerable loss of efficacy throughout the experiments. Accordingly, it is concluded that developing low-cost activated carbon to use BTEX vapor adsorption research could be practical and developments to overcome for utilization in air pollution control.

제2형 당뇨 마우스에서 밀웜 발효추출물의 혈당조절 효과

이해인(Hae-In Lee),Turkyilmaz Asli,이미경(Mi-Kyung Lee) 한국식품영양과학회 2023 한국식품영양과학회지 Vol.52 No.4

본 연구에서는 밀웜 발효추출물(MWF)이 당뇨병에 미치는 영향을 확인하기 위하여 db/db 마우스에서 혈당, 췌장의 인슐린 면역조직학적 변화 및 글리코겐 함량과 관련 유전자 발현을 측정하였다. MWF(200 mg/kg/d) 경구 보충 시 5주차부터 대조군에 비해 유의적으로 혈당이 낮아졌으며, 실험종료 시(6주) 내당능 장애 역시 개선되었다. MWF는 혈청의 인슐린 함량에는 유의적인 영향을 미치지 않았으나 MWF의 췌장 세포 내 랑게르한스섬 크기가 대조군에 비해 크고 뚜렷하였으며 베타세포의 인슐린 함량이 높았다. MWF는 간조직 내 포도당수송에 관여하는 Glut2 유전자와 글리코겐 합성에 관여하는 Gys2 유전자의 발현을 상향 조절하였으며, 이는 MWF군의 간조직 내 글리코겐 함량이 대조군에 비하여 유의적으로 높았음을 뒷받침한다. 이와 같이 MWF는 혈중의 포도당을 간조직으로 이동하여 저장 형태인 글리코겐으로 합성하여 혈당을 낮추는 데 기여하는 것으로 보인다. 따라서 MWF는 당뇨병을 예방하거나 개선하기 위한 식품소재로서의 가능성을 입증하였다. Mealworms (Tenebrio molitors larvae) have a high quality and quantity of amino acids and protein that have attracted attention as an alternative nutrition resource. This study examined the anti-hyperglycemic effects of fermented mealworm extract (MWF) in db/db mice. The mice were fed with or without MWF for six weeks. MWF was administered orally to the mice at 200 mg/kg body weight/d. MWF improved the fasting blood glucose level and glucose intolerance significantly compared to the control group. Insulin immunohistochemistry staining showed that the insulin levels of pancreatic β-cells in the MWF group were higher than those of the control group. MWF increased the hepatic glycogen content and the related gene levels, such as glucose transporter 2 and glycogen synthase 2. Thus, MWF lowered the blood glucose levels by up-regulating glucose uptake and glycogen synthesis-related gene expression. These results suggest that MWF can be used as a new natural anti-diabetic resource for type 2 diabetes.

Influence of various metal oxides on mechanical and physical properties of heat-cured polymethyl methacrylate denture base resins

Asar, Neset Volkan,Albayrak, Hamdi,Korkmaz, Turan,Turkyilmaz, Ilser The Korean Academy of Prosthodonitics 2013 The Journal of Advanced Prosthodontics Vol.5 No.3

PURPOSE. To evaluate the effect of various metal oxides on impact strength (IS), fracture toughness (FT), water sorption (WSP) and solubility (WSL) of heat-cured acrylic resin. MATERIALS AND METHODS. Fifty acrylic resin specimens were fabricated for each test and divided into five groups. Group 1 was the control group and Group 2, 3, 4 and 5 (test groups) included a mixture of 1% $TiO_2$ and 1% $ZrO_2$, 2% $Al_2O_3$, 2% $TiO_2$, and 2% $ZrO_2$ by volume, respectively. Rectangular unnotched specimens ($50mm{\times}6.0mm{\times}4.0mm$) were fabricated and drop-tower impact testing machine was used to determine IS. For FT, compact test specimens were fabricated and tests were done with a universal testing machine with a cross-head speed of 5 mm/min. For WSP and WSL, disc-shaped specimens were fabricated and tests were performed in accordance to ISO 1567. ANOVA and Kruskal-Wallis tests were used for statistical analyses. RESULTS. IS and FT values were significantly higher and WSP and WSL values were significantly lower in test groups than in control group (P<.05). Group 5 had significantly higher IS and FT values and significantly lower WSP values than other groups (P<.05) and provided 40% and 30% increase in IS and FT, respectively, compared to control group. Significantly lower WSL values were detected for Group 2 and 5 (P<.05). CONCLUSION. Modification of heat-cured acrylic resin with metal oxides, especially with $ZrO_2$, may be useful in preventing denture fractures and undesirable physical changes resulting from oral fluids clinically.

Influence of various metal oxides on mechanical and physical properties of heat-cured polymethyl methacrylate denture base resins

Turan Korkmaz,Hai Albayrak,Ilser Turkyilmaz,Neset Volkan Asar 대한치과보철학회 2013 The Journal of Advanced Prosthodontics Vol.5 No.3

PURPOSE To evaluate the effect of various metal oxides on impact strength (IS), fracture toughness (FT), water sorption (WSP) and solubility (WSL) of heat-cured acrylic resin. MATERIALS AND METHODS Fifty acrylic resin specimens were fabricated for each test and divided into five groups. Group 1 was the control group and Group 2, 3, 4 and 5 (test groups) included a mixture of 1% TiO2 and 1% ZrO2, 2% Al2O3, 2% TiO2, and 2% ZrO2 by volume, respectively. Rectangular unnotched specimens (50 mm × 6.0 mm × 4.0 mm) were fabricated and droptower impact testing machine was used to determine IS. For FT, compact test specimens were fabricated and tests were done with a universal testing machine with a cross-head speed of 5 mm/min. For WSP and WSL, discshaped specimens were fabricated and tests were performed in accordance to ISO 1567. ANOVA and Kruskal-Wallis tests were used for statistical analyses. RESULTS IS and FT values were significantly higher and WSP and WSL values were significantly lower in test groups than in control group (P<.05). Group 5 had significantly higher IS and FT values and significantly lower WSP values than other groups (P<.05) and provided 40% and 30% increase in IS and FT, respectively, compared to control group. Significantly lower WSL values were detected for Group 2 and 5 (P<.05). CONCLUSION Modification of heat-cured acrylic resin with metal oxides, especially with ZrO2, may be useful in preventing denture fractures and undesirable physical changes resulting from oral fluids clinically.

Radiation Exposure to Premature Infants in a Neonatal Intensive Care Unit in Turkey

Turan Olgar,Esra Onal,Dogan Bor,Nurullah Okumus,Yildiz Atalay,Canan Turkyilmaz,Ebru Ergenekon,Esin Koc 대한영상의학회 2008 Korean Journal of Radiology Vol.9 No.5

Objective: The aim of this work was to determine the radiation dose received by infants from radiographic exposure and the contribution from scatter radiation due to radiographic exposure of other infants in the same room. Materials and Methods: We retrospectively evaluated the entrance skin doses (ESDs) and effective doses of 23 infants with a gestational age as low as 28 weeks. ESDs were determined from tube output measurements (ESDTO) (n = 23) and from the use of thermoluminescent dosimetry (ESDTLD) (n = 16). Scattered radiation was evaluated using a 5 cm Perspex phantom. Effective doses were estimated from ESDTO by Monte Carlo computed software and radiation risks were estimated from the effective dose. ESDTO and ESDTLD were correlated using linear regression analysis. Results: The mean ESDTO for the chest and abdomen were 67 μGy and 65 μGy per procedure, respectively. The mean ESDTLD per radiograph was 70 μGy. The measured scattered radiation range at a 2 m distance from the neonatal intensive care unit (NICU) was (11-17 μGy) per radiograph. Mean effective doses were 16 and 27 μSv per procedure for the chest and abdomen, respectively. ESDTLD was well correlated with ESDTO obtained from the total chest and abdomen radiographs for each infant (R2 = 0.86). The radiation risks for childhood cancer estimated from the effective dose were 0.4 × 10-6 to 2 × 10-6 and 0.6 × 10-6 to 2.9 × 10-6 for chest and abdomen radiographs, respectively. Conclusion: The results of our study show that neonates received acceptable doses from common radiological examinations. Although the contribution of scatter radiation to the neonatal dose is low, considering the sensitivity of the neonates to radiation, further protective action was performed by increasing the distance of the infants from each other. Objective: The aim of this work was to determine the radiation dose received by infants from radiographic exposure and the contribution from scatter radiation due to radiographic exposure of other infants in the same room. Materials and Methods: We retrospectively evaluated the entrance skin doses (ESDs) and effective doses of 23 infants with a gestational age as low as 28 weeks. ESDs were determined from tube output measurements (ESDTO) (n = 23) and from the use of thermoluminescent dosimetry (ESDTLD) (n = 16). Scattered radiation was evaluated using a 5 cm Perspex phantom. Effective doses were estimated from ESDTO by Monte Carlo computed software and radiation risks were estimated from the effective dose. ESDTO and ESDTLD were correlated using linear regression analysis. Results: The mean ESDTO for the chest and abdomen were 67 μGy and 65 μGy per procedure, respectively. The mean ESDTLD per radiograph was 70 μGy. The measured scattered radiation range at a 2 m distance from the neonatal intensive care unit (NICU) was (11-17 μGy) per radiograph. Mean effective doses were 16 and 27 μSv per procedure for the chest and abdomen, respectively. ESDTLD was well correlated with ESDTO obtained from the total chest and abdomen radiographs for each infant (R2 = 0.86). The radiation risks for childhood cancer estimated from the effective dose were 0.4 × 10-6 to 2 × 10-6 and 0.6 × 10-6 to 2.9 × 10-6 for chest and abdomen radiographs, respectively. Conclusion: The results of our study show that neonates received acceptable doses from common radiological examinations. Although the contribution of scatter radiation to the neonatal dose is low, considering the sensitivity of the neonates to radiation, further protective action was performed by increasing the distance of the infants from each other.

Observations and experiences of pediatric surgeons working on the field in the first 7 days of the Kahramanmaras earthquake

Alparslan Kapisiz,Cem Kaya,Sibel Eryılmaz,Abdurrahman Azzam,Asli Sevimli,Ramazan Karabulut,Zafer Turkyilmaz,Kaan Sonmez 대한외과학회 2023 Annals of Surgical Treatment and Research(ASRT) Vol.105 No.2

Purpose: This study aims to share our experiences and problems, and to suggest solutions as pediatric surgeons who took part in the teams that went voluntarily to the region hit by the Kahramanmaraş earthquake during the first 7 days after the disaster. Methods: This study conveys our observations made at Kahramanmaraş Sütçüimam University Faculty of Medicine Hospital, where we worked as a volunteer team between February 7 and 14, 2023. Results: During the first few days, there were registration problems due to lack of electricity, water, and internet, as well as issues with sterile surgical environments. In the following days, a lack of auxiliary health personnel was revealed as the main difficulty. Conclusion: Since coordination is important when working as a team in the aftermath of an earthquake, staff from the same center should be deployed together if possible, and a team leader should be selected. Alternative recording systems should be established in case of power outages and computer problems. Secretaries, auxiliary health staff, and technicians should be included in the team in addition to doctors and nurses.