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Tai, Truong Ba,Lee, Sang Uck,Nguyen, Minh Tho The Royal Society of Chemistry 2016 Physical chemistry chemical physics Vol.18 No.17
<P>The cage-like structures containing octagonal holes are located as the lowest-lying isomers for the B-42(0/+). The presence of octagonal holes, which have been found for the first time, not only gives us new insight into the bonding motif, but also marks a breakthrough in the structural characteristics of boron clusters since they were never expected to be stable units for elemental clusters. These cages are composed of both delocalized sigma and pi electron systems that consequently make them aromatic and thermodynamically stable.</P>
Truong, A.-Tai,Kim, Byounghee,Kim, Somin,Kim, Moonjung,Kim, Jungmin,Kim, Seonmi,Yoon, Byoungsu International Bee Research Association [etc.] 2019 Journal of apicultural research Vol.58 No.5
<P> Israeli acute paralysis virus (IAPV) is one of the main pathogens involved in the collapse of honey bee colonies. However, because the virus exhibits a high level of genetic variation and some IAPV strains exhibit high degrees of homology with related viruses, the detection of IAPV in infected honey bees is relatively challenging. To address this obstacle, the aim of the present study was to develop a new detection method that relies on multiple detection sites within the IAPV genome and on Ultra-rapid real-time polymerase chain reaction (UR-qPCR). The new system simultaneously targeted a RNA-dependent RNA polymerase gene <I>(RdRp)</I> and two capsid genes (<I>VP3</I> and <I>VP1</I>). This multi-point PCR approach was highly efficient, with the ability to detect 100% of IAPV infections, and outperformed single-point PCR, which was only able to detect 86.96-95.6% of IAPV infections. Sequence analysis indicated that <I>RdRp</I> was more variable than the two capsid genes, and the specificity of the proposed method was demonstrated by the detection of IAPV from samples co-infected by IAPV and KBV. Importantly, both freezing-thawing RNA isolation and UR-qPCR could be performed in 27min and 40s. Therefore, the present provides an useful tool for the rapid identification of IAPV in apiaries. </P>
A-Tai Truong,Sedat Sevin,김선미,Mi-Sun Yoo,Yun Sang Cho,ByoungSu Yoon 대한수의학회 2021 Journal of Veterinary Science Vol.22 No.3
Background: The microsporidian parasite Nosema ceranae is a global problem in honeybee populations and is known to cause winter mortality. A sensitive and rapid tool for stable quantitative detection is necessary to establish further research related to the diagnosis, prevention, and treatment of this pathogen. Objectives: The present study aimed to develop a quantitative method that incorporates ultra-rapid real-time quantitative polymerase chain reaction (UR-qPCR) for the rapid enumeration of N. ceranae in infected bees. Methods: A procedure for UR-qPCR detection of N. ceranae was developed, and the advantages of molecular detection were evaluated in comparison with microscopic enumeration. Results: UR-qPCR was more sensitive than microscopic enumeration for detecting two copies of N. ceranae DNA and 24 spores per bee. Meanwhile, the limit of detection by microscopy was 2.40 × 104 spores/bee, and the stable detection level was ≥ 2.40 × 105 spores/bee. The results of N. ceranae calculations from the infected honeybees and purified spores by UR-qPCR showed that the DNA copy number was approximately 8-fold higher than the spore count. Additionally, honeybees infected with N. ceranae with 2.74 × 104 copies of N. ceranae DNA were incapable of detection by microscopy. The results of quantitative analysis using UR-qPCR were accomplished within 20 min. Conclusions: UR-qPCR is expected to be the most rapid molecular method for Nosema detection and has been developed for diagnosing nosemosis at low levels of infection.