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Hyaluronidase-inhibitory activities of glycosaminoglycans from Liparis tessellatus eggs
Ticar, B.F.,Rohmah, Z.,Mussatto, S.I.,Lim, J.M.,Park, S.,Choi, B.D. Applied Science Publishers 2017 Carbohydrate polymers Vol.161 No.-
Polysaccharide fractions isolated from L. tessellatus eggs were purified and eluted using the DEAE-sepharose fast flow column. These were collected, tested and pooled based on their sugars content: F1, F2, and F3 which contain 26.8, 23.3, and 20.2% sulfated glycans; 34.5, 38.2, and 45.0% uronic acids; and 23.5, 19.0, and 7.5% acetylhexosamines and hexosamines, respectively. Hyaluronidase inhibitory effects of the fractions are in the order F3>F2>F1>Ascorbic acid, with F3 having the highest inhibition among the fractions and that of the standard, ascorbic acid. The electrospray ionization tandem mass spectrometry (ESI-MS/MS) confirmed the presence of uronic acids on F3, which could be a <SUP>0,2</SUP>A<SUB>2</SUB> fragment plus loss of methyl group which is very common among non-methylated, sulfated disaccharides.
멍게껍질 카로테노이드의 색조 화장품 원료의 항산화, 항염증 기능성 평가
티카버나데스(Bernadeth Ticar),로마줄리야티(Zuliyati Rohmah),바터든뭉크자갈(Munkhjagal Bat-Erdene),박시향(Si-Hyang Park),최병대(Byeong-Dae Choi) 한국생물공학회 2013 KSBB Journal Vol.28 No.1
Carotenoids are fat-soluble red-orange colored pigments found in plants and seafood-derived products, including algae, seaweeds, and fish muscle. In this study, we have demonstrated the molecular mechanism underlying the antioxidants and anti-inflammatory properties of ascidian tunic carotenoids using mouse macrophage cell line (RAW 264.7). Cell viability was not affected by treatment of carotenoids < 10 μg/mL. This treatment also showed negative inhibition on lipopolysaccharide (LPS)-stimulated nitric oxide (NO) and cyclooxygenase-2 (COX-2). The DPPH radical scavenging activity of carotenoids was 47.2% at 100 mg/mL. It also has a potential reducing power (1.025) comparable with ascorbic acid (1.584). The ascidian tunic carotenoids would make a candidate for the commercially interesting biologically active cosmetic pigments.
Bernadeth F. Ticar,Zuliyati Rohmah,Si-Hyang Park,Yeung Joon Choi,Seok-Jeong Kang,Byeong-dae Choi 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1
The polysaccharides extracted from Liparis tesellatus eggs (LEP) and Pronase-digested LEP (LEP-PD) were tested for their anti-inflammatory, anti-cancer, and cytotoxic activities. The anti-inflammatory activities were determined using NO production by macrophage RAW 264.7 cell lines induced by lipopolysaccharide (LPS). Results showed that LEP-PD has an activity on all concentrations (0.01, 0.1, 1.0, 10.0, 100.0, and 200.0 ug/ml), whereas, no activity on all concentrations prepared for LEP. The anti-cancer activities of LEP and LEP-PD were evaluated using Human colon adenocarcinoma (HT-29) and Human stomach adenocarcinoma (AGS) cell lines. Results showed that both the LEP and LEP-PD have anti-carcinogenic effects on these specific cell lines. For the cytotoxic effects of LEP and LEP-PD, RAW 264.7 cell line was also used treated with the polysaccharides. The results showed that LEP and LEP-PD are non-toxic to the cells. From these data obtained, polysaccharides from Liparis tesellatus eggs can be used in the field of medicine upon further purification and elucidation of the structures of the glycans responsible for these promising activities.
Skin regeneration effect of the Glycosaminoglycans from <i>Liparis tessellatus</i> eggs
Lee, Jae-Joon,Ticar, Bernadeth F.,Rohmah, Zuliyati,Park, Si-Hyang,Kang, Dawon,Kang, Seok-Joong,Choi, Byeong-Dae ELSEVIER 2017 INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Vol.105 No.2
<P><B>Abstract</B></P> <P>A purified Glycosaminoglycans (GAGs) extract from <I>L. tessellatus</I> eggs was enzyme-hydrolyzed and then fractionated with DEAE-Sepharose column chromatography. The fractions were subsequently subjected to skin regeneration effects analysis against skin fibroblast (CCD-986sk) cell lines. Fraction 3 is evidently to have tyrosinase inhibition activity by 20.1% at concentration of 50mg/mL. DOPA oxidation assay, collagenase inhibition activity assay, fibroblast proliferation assay, and production of type I C-peptide assays were done to further proof the skin regeneration effect of GAGs fractions. Results revealed that fraction 3 has effective skin regeneration activities at a concentration of 200mg/mL.</P>
Antioxidant Activity of Nudibranch (Melibe viridis) Glycosaminoglycans
Zuliyati Rohmah,Bernadeth F. Ticar,Si-Hyang Park,Yeung Joon Choi,Seok-Jeong Kang,Byeong-dae Choi 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1
The glycosaminoglycans (GAGs) from Melibe viridis were extracted and optimized using Multifactorial design. Four factors (Temperature, pH, incubation period, and enzyme ratio) were varied in the optimization experiment. The design used three enzymes namely; Alcalase, Flavourzyme, and Protamex. The optimum results were obtained using Flavourzyme at pH 8.0, incubation temperature of 450C, for 15 hours, and an enzyme ratio of 1.5% relative to sample weight. The extracted Melibe GAGs (prepared at different concentrations) were tested for DPPH scavenging effect, it showed that, it has a comparable activity with standard, the Ascorbic acid. It also showed a reductive capability in a concentration-dependent manner. For the hydroxyl radical scavenging effect, the results showed that the activity is very near with that of the standard too, butylated hydroxyanisole (BHA). From the data obtained in this study, it can be inferred that, Melibe GAGs has a potential in the field of medicine as well as well as they can be used as dietary supplements with specific functional properties
Neri, Therese Ariane N.,Rohmah, Zuliyati,Ticar, Bernadeth F.,Palmos, Grace N.,Choi, Byeong-Dae The Korean Society of Fisheries and Aquatic Scienc 2019 Fisheries and Aquatic Sciences Vol.22 No.11
Sporophylls from sea mustard, Undaria pinnatifida, which are by-products in seaweed production industries, were taken from Hansan Island, Tongyeong, and Gijang, Busan, and investigated for their fucoidan content and corresponding antioxidant activities. The extracted fucoidan yield from sporophylls of sea mustard samples from Tongyeong (TF) and Gijang (GF) were 12.1% and 13.6%, respectively. The antioxidant activities assessment of TF and GF extracts showed relative significant difference between samples, suggesting effect of location on bioactivities of fucoidan samples. GF was 50-68% more effective than TF against DPPH and superoxide radicals samples which may be attributed to the difference in their degree of sulfation and monosaccharide composition. Meanwhile, TF exhibited greater scavenging activity against hydroxyl radicals compared to GF which could be due to protein impurities.
Atopic Skin Inflammation Improved by Using Extracts of Seaweed and Ascidians Tunic
B. Munkhjargal,Zuliyati Rohmah,Bernadeth Ticar,Lee Su Sun,Park Si Hyang,Choi Soon Ok,Byeong Dae Choi 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1
In a recent day, the environmental pollution caused by industrialization, usage of food additives increased and resulting the several types of atopic symptoms. We report the successful application of seaweed and ascidians tunic used as mediator for preventing skin inflammation of human. The current study was investigated to treat the skin inflammation; raw seaweed and ascidians tunic have been used and extracted. A natural ascidians tunic was extracted to get the glycosaminoglycans (GAGs). GAGs were mixed with the extraction of natural seaweed and it tested on the subject. As a result, it is found that the extraction of seaweed and ascidians tunic together refined material was not involved the cytokine inhibitory, although an extraction of seaweed was showed the anti-inflammation effect by the experiment. Ascidians tunic is represents an effect of cell generation and the mechanism of cytokines inhibition are rather than the inflammatory inhibition, at the same time the skin regeneration is considered greater effect. Finally, we found that the atopic skin inflammation can be improved and/or treated by using natural mixture extracts of the seaweed and ascidians tunic.
Therese Ariane Neri,Zuliyati Rohmah,Bernadeth F. Ticar,Byeong-Dae Choi 경상대학교 농업생명과학연구원 2020 농업생명과학연구 Vol.54 No.6
The effects of supplementing ESP-FM (Erythrobactor sp.), freshwater Chlorella (Chlorella sp.), and baker’s yeast (Saccharomyces cerevisiae) on the nutritional value and mass production of Moina macrocopa, which is used as a live feed for fish fry production, was investigated. Consequently, the effects of feeding the enriched M. macrocopa to the nutritional composition of larval rockfish (Sebastes schlegel) and carp (Cyprinus carpio) was also investigated. Maximum density of M. macrocopa was reached within 15-21 days after inoculation (0.5 to 22 individual/mL), at various temperatures, and either decreased or remained almost constantly thereafter. Protein content and amino acids composition of M. macrocopa were found to be influenced by their respective diets while lipid and ash contents did not considerably change. M. macrocopa fed with baker’s yeast were low in n-3 HUFA content, and those fed on the freshwater chlorella were high in the 18:2n-6 and 18:3n-3 HUFA content, and in cultures treated with ESP-FM were high content in n-3 HUFA. The utilization of M. macrocopa as a substitute fish feed for carp and rockfish showed the enrichment nutritional content.
Therese Ariane N. Neri,Zuliyati Rohmah,Bernadeth F. Ticar,Grace N. Palmos,최병대 한국수산과학회 2019 Fisheries and Aquatic Sciences Vol.22 No.4
Sporophylls from sea mustard, Undaria pinnatifida, which are by-products in seaweed production industries, were taken from Hansan Island, Tongyeong, and Gijang, Busan, and investigated for their fucoidan content and corresponding antioxidant activities. The extracted fucoidan yield from sporophylls of sea mustard samples from Tongyeong (TF) and Gijang (GF) were 12.1% and 13.6%, respectively. The antioxidant activities assessment of TF and GF extracts showed relative significant difference between samples, suggesting effect of location on bioactivities of fucoidan samples. GF was 50–68% more effective than TF against DPPH and superoxide radicals samples which may be attributed to the difference in their degree of sulfation and monosaccharide composition. Meanwhile, TF exhibited greater scavenging activity against hydroxyl radicals compared to GF which could be due to protein impurities.