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Biomolecular engineering for nanobio/bionanotechnology
Nagamune Teruyuki 나노기술연구협의회 2017 Nano Convergence Vol.4 No.9
Biomolecular engineering can be used to purposefully manipulate biomolecules, such as peptides, proteins, nucleic acids and lipids, within the framework of the relations among their structures, functions and properties, as well as their applicability to such areas as developing novel biomaterials, biosensing, bioimaging, and clinical diagnostics and therapeutics. Nanotechnology can also be used to design and tune the sizes, shapes, properties and functionality of nanomaterials. As such, there are considerable overlaps between nanotechnology and biomolecular engineering, in that both are concerned with the structure and behavior of materials on the nanometer scale or smaller. Therefore, in combination with nanotechnology, biomolecular engineering is expected to open up new fields of nanobio/bionanotechnology and to contribute to the development of novel nanobiomaterials, nanobiodevices and nanobiosystems. This review highlights recent studies using engineered biological molecules (e.g., oligonucleotides, peptides, proteins, enzymes, polysaccharides, lipids, biological cofactors and ligands) combined with functional nanomaterials in nanobio/bionanotechnology applications, including therapeutics, diagnostics, biosensing, bioanalysis and biocatalysts. Furthermore, this review focuses on five areas of recent advances in biomolecular engineering: (a) nucleic acid engineering, (b) gene engineering, (c) protein engineering, (d) chemical and enzymatic conjugation technologies, and (e) linker engineering. Precisely engineered nanobiomaterials, nanobiodevices and nanobiosystems are anticipated to emerge as next-generation platforms for bioelectronics, biosensors, biocatalysts, molecular imaging modalities, biological actuators, and biomedical applications.
Protein Microarrays and Their Applications
Bum Hwan Lee,Teruyuki Nagamune 한국생물공학회 2004 Biotechnology and Bioprocess Engineering Vol.9 No.2
In recent years, the importance of proteomic works, such as protein expression, detection and identification, has grown in the fields of proteomic and diagnostic research. This is because complete genome sequences of humans, and other organisms, progress as cellular processing and controlling are performed by proteins as well as DNA or RNA. However, conventional protein analyses are time-consuming; therefore, high throughput protein analysis methods, which allow fast, direct and quantitative detection, are needed. These are so-called protein microarrays or protein chips, which have been developed to fulfill the need for high-throughput protein analyses. Although protein arrays are still in their infancy, technical development in immobilizing proteins in their native conformation on arrays, and the development of more sensitive detection methods, will facilitate the rapid deployment of protein arrays as high-throughput protein assay tools in proteomics and diagnostics. This review summarizes the basic technologies that are needed in the fabrication of protein arrays and their recent applications.
Charge trap in self-assembled monolayer of cytochrome b562-green fluorescent protein chimera
최정우,남윤석,이범환,안동준,Teruyuki Nagamune 한국물리학회 2006 Current Applied Physics Vol.6 No.4
The self-assembly layer consisting of fusion protein is investigated in molecular-scale for the construction of bioelectronic device.Cytochromeb562 and green uorescent protein were used as an electron acceptor and a sensitizer in the molecular layer by mim-icking the photosynthesis. Self-assembled monolayer of fusion protein was formed on Au coated glass. The formation of fusion pro-tein layer onto the Au substrate was observed by the surface plasmon resonance measurement. The surface of fusion protein layerwas observed and analyzed by the scanning tunneling microscopy observation. For embodiment of the molecular electronic device,molecular arrays of fusion protein SA layer were formed by micro contact printing. Surface charge distribution of fusion protein SAlayer was measured to conrm an electrical conductivity by electrostatic force microscopy observation.
Bumhwan Lee,Akihiko Tajima,Joonwan Kim,Yutaka Yamagata,Teruyuki Nagamune 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.1
In this study, antibody-based protein microarrays for high-throughput immunoassay were fabricated on an aldehydemodified indium-tin oxide glass plate using the electrospray deposition (ESD) method and their characteristics were evaluated immunochemically. The microarrays were also integrated into microfluidic chips with a polydimethylsiloxane (PDMS) micro-channel to detect human cytokines, which were quantitatively analyzed with a high resolution chargecoupled device. Simultaneous detection of various antigens was performed using the microarrays with considerable sensitivity (ca. 100 pg/mL). The results of this study indicate that microfluidic chip comprising a protein microarray formed by the ESD method and a PDMS micro-channel could be easy to handle, and offers high-throughput detection of molecular biomarkers.
Yoo, Si-Youl,Min, Junhong,Haga, Tomoaki,Hirakawa, Hidehiko,Nagamune, Teruyuki,Choi, Jeong-Woo American Scientific Publishers 2017 Journal of nanoscience and nanotechnology Vol.17 No.8
<P>Various kinds of biologic gates have been developed to realize biocomputing system with enzyme reactions. However, logic gate with summation function based on putidaredoxin reductase and cytochrome c has not been reported yet. Here, we developed biologic gate with summation function. To fabricate the biologic gate system, putidaredoxin reductase was immobilized on two gold electrodes for input system and cytochrome c was immobilized on one gold electrode for reading output signal. Then polydimethylsiloxane based microfluidic chip was fabricated on the gold electrodes and each electrode was connected with microfluidic channels. To operate the biologic gates, two AND gates with putidaredoxin reductase and one OR gate with cytochrome c was checked by confirming enzyme reactions firstly. And then, we checked summation function with fabricated two AND gates and one OR gate. The benzoquinone injected to each putidaredoxin reductase was acted as input and it was defined to '0', '1' and '2' by different concentrations. Two input signals were flowed to cytochrome c and summated signal was checked by cyclic voltammetry. The summated output signals were defined from '0' to '4' with various thresholds of current intensity. Proposed enzyme logic gate with summation function can be applied to bioprocessing and biocomputing system.</P>