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Tania Parvin,Mim Binti Akhlas,Fatema Khatun,Anzuman Akter,Md. Al Amin,Muhammad Torequl Islam,Razina Rouf 경희대학교 융합한의과학연구소 2019 Oriental Pharmacy and Experimental Medicine Vol.19 No.4
This study aimed at phytochemical analysis and evaluation of toxic and protective efects of the crude aqueous extract of Typhonium trilobatum. Toxicity analysis was done in Allium cepa, while anti-infammatory, and membrane stabilizing and clotlysis capacity were tested by using egg albumin and human erythrocytes, respectively. The results suggest that T. trilobatum aqueous extract contains alkaloids, glycosides, tannins, saponins, favonoids and reducing sugars. The crude extract exhibited toxicity in A. cepa in a time and concentration dependent manner. The highest root growth inhibition was observed at exposure time 72 h in 20% (v/v) concentration extract of the herb. The extract at low concentration (2.5 and 5%) reduced the root growth inhibition capacity at 48 h in comparison to 24 and 72 h, possibly by adaptation of DNA damage profle in the eukaryotic test system (A. cepa), which was further confrmed in anti-infammatory and membrane stabilizing, and anti-atherothrombosis test. In the latter cases, the extract at 0.02–0.08% (v/v) signifcantly (p<0.05, p<0.01, p<0.001) inhibited protein denaturation, membrane lysis, while enhanced clotlysis capacity in a concentration-dependent manner. In conclusion, the extract exerted toxic efects at high concentrations and protective efects at low concentrations. T. trilobatum may be one of the potential sources of phytotherapeutic agents, especially for the treatment of infammatory and cardiovascular diseases and disorders.
Tania Afroz,이호선,전영아,성정숙,이주희,Awraris Derbie Assefa,노재정,황애진,허온숙,노나영,이재은,이명철 한국작물학회 2019 Journal of crop science and biotechnology Vol.22 No.2
Sclerotinia rot and Phytophthora blight are devastating diseases of perilla that can cause huge loss of perilla production. Screening disease resistant genetic resources is necessary to develop disease-resistant cultivars and conduct related research. The objective of this study was to find suitable inoculation methods for screening of Sclerotinia rot and Phytophthora blight in perilla germplasm. Three different inoculation methods [i.e. detached leaf (agar plug placed on start point of vein, 1/3 point of leaf vein and front side, back side), stem tip, and soil drenching] with three-growth stages (two, four & six-leaf stage) at two different temperatures (20 & 25oC) were assayed for their effectiveness for screening using IT 229019 and Sang-yeop perilla germplasm. Based on disease lesion, 20oC in two-leaf stage with detached leaf method (start point of vein) was found to be an efficient method for screening of Sclerotinia rot whereas 25oC in two-leaf stage with stem tip was an effective method for screening of Phytophthora blight in perilla germplasm. However, stem tip method was laborious as it required more time. Therefore, detached leaf method (start point of vein and back side) was a simple and rapid method that could be preferably used for screening of Sclerotinia rot and Phytophthora blight in perilla germplasm.
Preference Choice Survey of Pork Chop by French and Korean Consumers
Tania M. Ngapo,Soo-Hyun Cho,Beom-Young Park,Jin-Hyeong Kim,Jong-Moon Lee,Sung Ki Lee 한국축산식품학회 2004 한국축산식품학회지 Vol.24 No.1
Digital photographs of 16 pork chops were each modified to give 16 treatments, such as two levels each of fat cover, color, marbling and drip, giving a total of 256 images. Consumers from three locations in France and six locations in Korea selected their preferred chop from 16 treatments in different chops and repeated this selection process eight times from different groups of chops, respectively. Respondents were asked to complete a questionnaire on socio-demographic information. Both the results of the French and Korean surveys gave four clusters of consumers, but they were not the same in terms of the choice strategies used, or in terms of their relationships with the socio-demographic items. The interesting apparent similarities (such as, both color levels being equally important to consumers in both countries) and differences(such as, the strong preference for lean meat in France; no fat preference in Korea) suggest a need to compare the results from the two countries. However, due to the difference of the socio-demographic make-up of the consumer panels from the two countries, a simple and direct comparison of the clusters based on choice and their relationships with the questionnaire items is not possible, but is currently being explored.
Tania Coppa,Maria Claudia Lazzè,Ornella Cazzalini,Paola Perucca,Roberto Pizzala,Livia Bianchi,Lucia Anna Stivala,Luca Forti,Cristina Maccario,Vanio Vannini,Monica Savio 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.10
Resveratrol inhibits endothelin-1, a vascular tension regulator. We synthesized the resveratrol analogue 4,4′-dihydroxy-trans-stilbene with 2 hydroxyl groups in the 4 and 4′ position to obtain a molecule more active than resveratrol (3,4′,5-trihydroxy-trans-stilbene). The results demonstrate that 4,4′-dihydroxy-trans-stilbene led to a significant decrease in total endothelin-1 secretion and in endothelin-1 messenger RNA (mRNA) levels in human endothelial cells. In addition, resveratrol and its analogue decreased endothelin-converting enzyme-1 mRNA levels and further reduced the activity of the enzyme. 4,4′-dihydroxy-trans-stilbene was more active than resveratrol because the new molecule exerted greater activity at the level of endothelin synthesis and conversion, even at a lower concentration. Although 4,4′-dihydroxy-trans-stilbene and resveratrol inhibited formation of reactive oxygen species and lipid peroxidation, the treatment of cells with different oxidant agents did not modify the endothelin-1 release. This finding suggests that the inhibition of endothelin-1 secretion is independent of the antioxidant properties of the 2 compounds. On the basis of these results, the resveratrol analogue 4,4′-dihydroxy-trans-stilbene could be a promising chemopreventive agent against cardiovascular diseases.
Tania Perez-Perez,Arletty Funcada-Martinez,Ania Cabrera-Diaz,Luis E. Guerra-Diaz,Deny Oliva-Merencio,Zhenia Milan,Ileana Pereda-Reyes 대한환경공학회 2022 Environmental Engineering Research Vol.27 No.5
Large pig farms generate enormous volumes of wastewater without proper treatment before discharge. High-rate anaerobic reactors as the expanded granular sludge bed (EGSB) are rarely reported for that purpose. In this study, an EGSB reactor with Cuban natural zeolite addition was evaluated for treating piggery wastewaters at lab scale. This study was conducted at different organic loading rate (OLR) ranging from 0.32 to 6.13 gCOD/Ld with a constant hydraulic retention time (HRT) of 12 h. The evolution of several control and operational parameters as the soluble chemical oxygen demand (COD) demonstrated a proper bioreactor performance to treat piggery wastewater. The results showed that the addition of natural zeolite at 40 gzeo/Lreactor in EGSB reactor results in COD efficiencies over 70%, despite of the fluctuations of fed wastewater characteristics. The kinetic study demonstrates that, the modified Stover-Kincannon model was found to be appropriate for EGSB reactor. The effluent soluble COD was predicted with high determination coefficient. The maximum removal rate constant (Umax) and saturation value constant (KB) were reported for a first time for the EGSB reactor with values of 10.2 and 11.8 gCOD/Ld, respectively. Methane yield for both, batch and continues experiments, were in the range from 214.5 to 299.8 mLCH4/gCOD.
Tania R. Dias,Ashok Agarwal,Peter N. Pushparaj,Gulfam Ahmad,Rakesh Sharma 대한남성과학회 2020 The World Journal of Men's Health Vol.38 No.2
Purpose: Patients with non-seminoma testicular cancer (NSTC) cancer can be subfertile or infertile, and present reduced sperm quality, but the underlying mechanisms are unknown. The aim of this study was to compare the sperm proteome of patients with NSTC, who cryopreserved their sperm before starting cancer treatment, with that from healthy fertile men.Materials and Methods: Semen volume, sperm motility and sperm concentration were evaluated before the cryopreservation of samples from patients with NSTC (n=15) and the control group (n=15). Sperm proteomic analysis was performed by liquid chromatography-tandem mass spectrometry and the differentially expressed proteins (DEPs) between the two groups were identified using bioinformatic tools.Results: A total of 189 DEPs was identified in the dataset, from which five DEPs related to sperm function and fertilization were selected for validation by Western blot. We were able to validate the underexpression of the mitochondrial complex subunits NADH:Ubiquinone Oxidoreductase Core Subunit S1 (NDUFS1) and ubiquinol-cytochrome C reductase core protein 2 (UQCRC2), as well as the underexpression of the testis-specific sodium/potassium-transporting ATPase subunit alpha-4 (ATP1A4) in the NSTC group. Conclusions: Our results indicate that sperm mitochondrial dysfunction may explain the observed decrease in sperm concentration, total sperm count and total motile count in NSTC patients. The identified DEPs may serve as potential biomarkers for the pathophysiology of subfertility/infertility in patients with NSTC. Our study also associates the reduced fertilizing ability of NSTC patients with the dysregulation of important sperm molecular mechanisms.