Growth Inhibition of Colon Cancer through Inactivation of STAT3 Pathway by IL-10 and IL-1ra Released from Murine Macrophage

Jin Tae Hong, Dohee Won1, Mi Hee Park, Sun Mi Kown, Miran Jo, Sang-Yoon Nam, Beom Jun Lee, Young Won Yun, Ki-Wan Oh, Sang Bae Han 충북대학교 동물의학연구소 2011 Journal of Biomedical and Translational Research Vol.12 No.4

The objective of this study was to determine the effect of macrophages on growth of human colon cancer cells. The results showed that co-culture of colon cancer cells with macrophages inhibited the growth of colon cancer cells (HCT116 and SW620) depending on the number of macrophages, RAW 264.7 cells, and activated THP-1 cells accompanied by down regulation of pSTAT3 in cancer cells. We also found that expression and release of cancer cell growth inhibitory cytokines, IL-1 receptor antagonist (IL-1ra) and IL-10, was increased in macrophages. Blocking of the STAT3 pathway with specific inhibitor and siRNA of STAT3 abolished the growth of colon cancer cells and expression of IL-1ra and IL-10. In addition, neutralization of IL-1ra and IL-10 with antibodies resulted in reversal of macrophage-induced inhibition of cancer cell growth. These data showed that IL-1ra and IL-10 released from macrophages inhibit growth of colon cancer cells through inhibition of the STAT3 pathway

동북아 피더네트워크의 Win-win전략에 관한 연구

장우준(Woo-jun jang),전찬용(Chan-Yong jeon),김태원(Tae-Won Kim),곽규석(Kyu-Seok Kwak),남기찬(Ki-Chan Nam) 한국항해항만학회 2006 한국항해항만학회 학술대회논문집 Vol.3 No.1

최근 중국은 양산항을 개장하여 선사 유치를 위한 다양한 혜택을 제공하고, 일본은 "수퍼중추항만 육성계획“을 세워 항만의 효율성을 바탕으로 비용을 낮추려는 노력을 하고 있다. 또한 선박의 대형화에 의한 중심항만(Hub Port) 위주의 기항과 북중국의 대량 물량 발생지역에 대한 직기항 경향으로 동북아 항만간의 경쟁이 더욱더 치열해 지고 있으며 동북아 역내 피더네트워크 강화의 필요성이 제기되고 있다. 따라서, 본 연구에서는 유럽 피더네트워크 전략 사례를 바탕으로 동북아 피더시장 현황을 파악하고 동북아 피더네트워크 구조를 분석함으로써 동북아 역내 항만 및 선사간의 과당경쟁을 지양하고 서로 Win-Win할 수 있는 터미널의 전략적 활용, 선사간 제휴 확대, 항만간 협력 강화를 위한 구체적인 전략을 제시하였다. Recently, China opened Yangshan port and provides a variety of benefits for shipping companies. Japan plans to reduce logistics costs and improve efficiency through the established "super major ports" project. According to the "hub and spokes" system, the number of direct calls toward North-Chinese ports are increasing. This changing environment has negative effects on the amount of transshipment cargos in Korean ports. In order to make a hub port of a domestic port, it is necessary to develop northeast Asian feeder networks. Therefore, this paper analyzes the structure of northeast Asian feeder networks through the case study of European feeder networks strategy. Finally, we suggests the plans to reinforce feeder networks, which are strategic terminal operation, strategic alliances between shipping companies and port co-operation, for win-win strategy in the competitive northeast Asian market.

석탄 가스화를 통한 전력 생산과 DME 병산 공정에 대한 기초 경제성 분석

유영돈 ( Young Don Yoo ),김수현 ( Su Hyun Kim ),조원준 ( Won Jun Cho ),모용기 ( Yong Gi Mo ),송용택 ( Tae Kyong Song ) 한국화학공학회 2014 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.52 No.6

The key for the commercial deployment of IGCC power plants or chemical (methanol, dimethyl ether, etc.) production plants based on coal gasification is their economic advantage over plants producing electricity or chemicals from crude oil or natural gas. The better economy of coal gasification based plants can be obtained by co-production of electricity and chemicals. In this study, we carried out the economic feasibility analysis on the process of co-producing electricity and DME (dimethyl ether) using coal gasification. The plant’s capacity was 250 MW electric and DME production of 300,000 ton per year. Assuming that the sales price of DME is 500,000 won/ton, the production cost of electricity is in the range of 33~58% of 150.69 won/kwh which is the average of SMP (system marginal price) in 2013, Korea. At present, the sales price of DME in China is approximately 900,000 won/ton. Therefore, there are more potential for lowering the price of co-produced electricity when comparing that from IGCC only. Since the co-production system can not only use the coal gasifier and the gas purification process as a common facility but also can control production rates of electricity and DME depending on the market demand, the production cost of electricity and DME can be significantly reduced compared to the process of producing electricity or DME separately.

형질전환 제초제저항성 벼 밀양 204호의 농업적 특성

정응기,이기환,원용재,박향미,전남수,최준호,구연충,한창덕,은무영,김태산,남민희,Jeong Eung-Gi,Yi Gi-Hwan,Won Yong-Jea,Park Hyang-Mi,Cheon Nam-Soo,Choi Jun-Ho,Ku Yeon-Chung,Han Chang-Deok,Eun Mu-Yeong,Kim Tae-Sas,Nam Min-Hee 한국식물생명공학회 2005 식물생명공학회지 Vol.32 No.2

밀양 204호는 작물과학원 영남농업연구소 생명공학연구팀이 직파적응 제초제저항성 벼 품종을 육성할 목적으로 '98년 bar 유전자를 동진벼에 아그로박테리움법으로 형질 전환하여 제초제저항성 벼를 양성하였다. '98/'99년 동계에 단간 내도복 양질인 주남벼를 인공교배하여 약배양 등 육종프로그램에 적용하여 우량계통을 선발하고 밀양 204호로 계통명을 부여하였다. GM 벼와 Non-GM 벼의 농업적 특성을 UPOV 및 국립종자관리소의 품종등록 기준에 따른 농업적특성 중 차이가 있었던 것은 엽색도, 지엽의 형태, 간장, 수당립수 등 이었다. This study was conducted to investigated the major characteristics of genetically modified rice of 'Milyang 204' originated from Dongjinbyeo compared to a non-transgenic rice varieties Dongjinbyeo and Jun-ambyeo. Basta resistant transgenic rice lines carrying bar gene produced by the Yeongnam Agricultural Research Institute were evaluated for their agronomic characters. The transgenic Japonica rice of 'Milyang 204' showed inferior phenotypic traits compared to a non-transgenic rice variety Dongjinbyeo and Junambyeo. On the basis of UPOV (Union Internationale Pour la Protaection des Obtentions Vegetables) and NSMO(National Seed Management Office) the transgenic 'Milyang 204' showed difference in some traits out of some agronomic traits, such as leaf color, angle of flag leaf, number of spikelets, culm length, white core and white belly compared to the non-transgenic varieties rice.

Antioxidative Activity of the Hydrolytic Enzyme Treated Sorbus commixta Hedl.and its Inhibitory Effect on Matrix Metalloproteinase-1 in UV Irradiated Human Dermal Fibroblasts

Jun-Tae Bae,Gwan-Sub Sim,Jin-Hwa Kim,표형배,Jong-Won Yun,Bum-Chun Lee 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.9

Superoxide radical scavenging activity and DPPH radical scavenging activity were assessed in order to evaluate the antioxidant effect of the Sorbus commixta Hedl. extract (SCoE). SCoE was also treated with several carbohydrate-hydrolytic enzymes that significantly increased the total phenol and flavonoid composition of SCoE. The enzymatically treated SCoE was then assessed for antioxidative activity. The most efficient radical scavenging activity was observed when SCoE was treated with -glucanase. The radical scavenging activity of β-glucanasetreated SCoE (β-GSCoE) enhanced the viability of human dermal fibroblasts (HDFs) exposed to ultraviolet (UV) light. The intracellular reactive oxygen species (ROS) scavenging activity of β-GSCoE was assessed using UVB (20 mJ/cm2)-irradiated HDFs. UVB irradiation increased dichlorofluorescein (DCF) fluorescence, which was measured by a 5-(6-)chloromethyl-2',7'- dichlorodihydrofluorescein diacetate (CM-H2DCFDA). DCF-fluorescence was significantly decreased in the β-GSCoE-containing culture medium, suggesting that β-GSCoE scavenges free radicals. The protective effect was further verified by assessing the expression of matrix metalloproteinase-1 (MMP-1) in UVA-irradiated HDFs. The treatment of UVA-irradiated HDFs with β-GSCoE resulted in a dose-dependent decrease in the expression level of MMP-1 protein and mRNA. These results suggest that β-GSCoE may mitigate the effects of photoaging in skin by reducing UV-induced adverse skin reactions.

Combined effects of p73 and MDM2 polymorphisms on the risk of lung cancer

Jun, Hee Jung,Park, Sun Ha,Lee, Won Kee,Choi, Jin Eun,Jang, Jin Sung,Kim, Eun Jin,Cha, Sung Ick,Kim, Dong Sun,Kam, Sin,Kim, Chang Ho,Kang, Young Mo,Jung, Tae Hoon,Park, Jae Yong Alan R. Liss, Inc 2007 Molecular carcinogenesis Vol.46 No.2

<P>p73, a structural and functional homologue of p53, plays an important role in modulating cell-cycle control and apoptosis. MDM2 represses the transcriptional activity of p73 and thus attenuates its activity. Based on the interaction between p73 and MDM2 in cell-cycle control and apoptosis, we investigated the association between p73 G4C14-to-A4T14 and MDM2 309T > G polymorphisms, alone and in combination, on the risk of lung cancer in a Korean population. The p73 and MDM2 genotypes were determined in 582 lung cancer patients and in 582 healthy control subjects who were frequency-matched for age and gender. The p73 AT/AT and MDM2 309 GG genotypes were associated with a nonsignificant increased risk of lung cancer (adjusted odds ratio [OR] = 1.37, 95% confidence interval [CI] = 0.83–2.24; and adjusted OR = 1.29, 95% CI = 0.92–1.80, respectively), compared with their wild-type genotypes, respectively. When the p73 and MDM2 polymorphisms were combined, the risk of lung cancer increased in a dose-dependent manner as the number of variant alleles increased (P<SUB>trend</SUB> = 0.01). Subjects with three or four variant alleles were at a significantly increased risk of lung cancer (adjusted OR = 1.74, 95% CI = 1.11–2.74, P = 0.02) compared to subjects with zero variant allele. These results suggest an additive effect of the p73 and MDM2 variant alleles on an increased risk of lung cancer. © 2006 Wiley-Liss, Inc.</P>

Effect of hemin and ascorbic acid on colonic pre-neoplastic lesions induced by azoxymethane in mice

Jun Ho Kim,Tae Ryong Kim,Hyo Suk Choi,Sung June Kim,Jae Hwang Jeong,Sang Yoon Nam,Young Won Yun,Beom Jun Lee 한국예방수의학회 2017 예방수의학회지 Vol.41 No.4

Colorectal cancer (CRC) is the third most prevalent cancer in the world, and heme iron is known to promote the CRC in an animal model. This study was conducted to investigate the effects of ascorbic acid in the presence of hemin on the formation of pre-neoplastic lesions induced by azoxymethane (AOM)/disodium sulfate (DSS) in mice. After acclimation for 1 week, five-week old mice received three s.c. injections (0-2 weeks of the experiment) of AOM [10 mg/kg body weight (BW)] weekly and were treated with 2% DSS in drinking water for the next week to induce aberrant crypt foci (ACF). All animals were fed the AIN-76A purified rodent diet for experimental period of 6 weeks. Experimental groups were then divided into three groups: carboxymethylcellulose (CMC) alone (control), CMC + Hemin, CMC + Hemin + ascorbic acid (AA). The CMC was used as a solvent for hemin. The daily doses were 534 mg/kg BW hemin and 246 mg/kg BW ascorbic acid administered orally. After the colonic mucosa were stained with methylene blue, aberrant crypt foci (ACF), aberrant crypt (AC) and polyps were counted. Lipid peroxidation in liver was evaluated by the thiobarbituric acid-reactive substances (TBARS) assay. The numbers of ACF, AC and large ACF (≥4 AC/ACF) per colon increased in the hemin group compared to the control group, while they decreased significantly in the hemin + ascorbic acid group compared to the control group or hemin group (p<0.01). The number of polyps/colon in the hemin + AA group was significantly decreased compared to the hemin group (p<0.05). In the liver, the TBARS value of the hemin group was significantly higher than that of the control group (p<0.01). Additionally, the TBARS value of the hemin + AA group decreased slightly compared to that of the hemin group. Taken together, these results suggest that hemin can promote colon carcinogenesis in a mouse model and that ascorbic acid has a protective effect against hemin-promoted colon carcinogenesis.

Cu2O based Photostable Photoanodes by the Au Passivation for High-efficiency Photoelectrochemical Applications

Tae Won Lee,Hee Jun Kim,Jeong Min Baik 한국표면공학회 2018 한국표면공학회 학술발표회 초록집 Vol.2018 No.11

FCT 11 : Efficacy of laser therapy in early postoperative thyroidectomy scar

( Jun Hwan Kim ),( Joon Ho Shim ),( Se Won Park ),( Hyun Tae Shin ),( Ji Ho Park ),( Jong Hee Lee ),( Dong Youn Lee ),( Joo Heung Lee ),( Jun Mo Yang ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2

Background: Interest in surgical scars is on the rise with increasing frequency of surgical procedures. The site of thyroidectomy is the anterior neck, which is also a prominently exposed part of the body, where postoperative scarring can cause patients` distress. Various types of lasers are effectively used to improve the appearance of scars but optimal treatment time has not been established. Objectives: To evaluate the efficacy and safety of laser therapy in early postoperative thyroidectomy scars. Methods: We reviewed the medical records of 43 thyroidectomy patients who were divided into two groups based on treatment time. Early treatment group started laser therapy within the first month and late treatment group at least 1 month after the thyroidectomy. Each group was treated with 595nm Pulse dye and fractional laser till achieving cosmetic acceptable outcome (Delta VSS score > 4). We used the Vancouver Scar Scale (VSS). Evaluations were performed based on medical photograph which was taken before each treatment. Results: Mean VSS scores were statistically significantly lower after laser treatment (P<0.01).Early treatment group require fewer treatment sessions and less expense than Late treatment group (P<0.01). Conclusion: Using lasers as prophylaxis against scarring would require fewer treatment sessions and less expense than using lasers to treat mature scars.

P086 : Adalimumab therapy for psoriasis in korean patients: an interim report

( Jun Hwan Kim ),( Joon Ho Shim ),( Se Won Park ),( Hyun Tae Shin ),( Ji Ho Park ),( Jong Hee Lee ),( Dong Yoon Lee ),( Joo Heung Lee ),( Jun Mo Yang ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2

Background: Adalimumab, the first fully human tumor necrosis factor alpha (TNF-a) monoclonal antibody is widely used to manage moderate to severe chronic plaque psoriasis. Up to now, data are lacking on the use of adalimumab for psoriasis treatment in the Korean. Objectives: To estimate the efficacy and safety of adalimumab in Korean psoriasis patients and compare this results with other studies. Methods: Total 10 patients treated with adalimumab were enrolled. Medical data was retrospectively collected from March 2011 to July 2013. All patients were injected adalimumab 80 mg given subcutaneously the first week, followed by 40 mg subcutaneously given the next week and then every other week. Data including Psoriasis Area and Severity Index (PASI), and any adverse event were recorded every visit. Results: Eight patients of 9, receiving adalimumab 16 weeks, achieved PASI 75 at 16 week. In the remaining 1 patient, a patient achieved PASI 75 at 18 week and another patient discontinued adalimumab due to upper respiratory infection, any adverse event causally related to the treatment was not reported in the 16 weeks treatment period. In this study, overall 89% (n=8/9) of patients achieved PASI 75 at 16 week and all patients well tolerated to adlimumab treatment. And PASI 75 and 90 at 74 week were 100% (n=5/5) and 80% (n=4/5) respectively. Conclusion: Ongoing study will include more patients and determine long term efficacy and safety of adalimumab treatment in the Korean psoriasis patients.