Extracellular Proteinase를 생산하는 효모의 분리동정과 효소의 생산

김창화,이태형,유춘발,진익렬 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

토양 및 농업용수와 하수 등으로부터 분리한 237주의 효모를 대상으로 단백질 분해력이 있는 효모 13주를 분리한 후, 알카리 조건에서 활성이 우수한 효모 TH65의 형태학적 및 생리생화학적 특성을 조사하고, 그 결과를 Saccharomycopsis lipolytica KCCM 12495 및 S. lipolytica KCCM 35426와 비교한 바, 발효성이 없고 대부분의 당류와 nitrate를 이용하지 못하는 등 대조 균주와 거의 동일한 특성을 보임에 따라 분리균주를 Yarrowia lipolytica TH65로 동정하였다. 분리효모의 protease 생산성을 조사한 결과, 탄소원으로는 glycerol과 glucose가 좋았고, glucose 농도는 0.5%가 적당하였다. 질소원으로는 0.6% skim milk에서 가장 많은 효소가 유도되었고, 아미노산에 의한 영향을 조사한 결과는 cysteine, cystine 및 tryptophane에 의해 효소 생산성이 감소되는 것으로 나타났다. Yeast extract 농도의 영향을 조사한 결과는 0.1~0.5%에서 양호하였으나, 첨가된 대부분의 무기염과 인산염은 효소생산성을 증가시키지 못하는 것으로 나타났다. 배양초기 pH는 9~11에서 가장 좋았고, 배양온도는 18℃에서 가장 좋았으며, 배양시간에 따른 효소생산성은 36시간에서 최고치를 나타내었다. A yeast strain TH65 producing a high level of proteinase under alkaline condition was isolated, and identified as Yarrowia lipolytica by morphological, physiological, and biochemical characteristics. In proteinase productivity, glycerol and glucose among tested carbon sources were very effective, and optimum concentration of glucose was 0.5%. Skim milk was found to be most effective nitrogen source in productivity, and its optimum concentration was 0.6%. But, cysteine, cystine and tryptophane decreased the proteinase productivity. Yeast extract was relatively effective at the range of 0.1~0.5%. The yeast showed maximum production of proteinase at 18℃, pH 9~11, and cultivation time of 36 hours.

충남대학교 공업기술 계열 교사 연수 프로그램에 대한 만족도 분석

류창열,최유현,김판욱,김기수,김태훈,이창훈 忠南大學校 工業敎育硏究所 2005 論文集 Vol.27 No.1

The purpose of this study was to analyze the educated teachers' satisfaction of the in-service education programs of industrial technical and technology teachers in Chungnam National University. This study was carried out through survey to accomplish the purpose. The number of questionnaires collected was 86. This study used the SPSS program to conduct a descriptive analysis of the data. The results of this study were as follows: (1) The satisfaction about 'teachers' participation', 'utilization of audiovisual materials and instruments' and 'level of professor and lecture' was relatively high. (2) The satisfaction about 'environments of in-service education programs', 'evaluation of achievement', and 'efficacy of lectures' was relatively low.

PASS (polyamic acid alkylamine salts) LB막의 유전 특성

강도열,김태완,박준수 弘益大學校 科學技術硏究所 1994 科學技術硏究論文集 Vol.4 No.-

Dielectric properties of PAAS LB films were studies in a frequency range of 10 Hz to 13 MHz. Y-type films were made under a surface pressure of 30mN/m using Kuhn type apparatus. It was found that there is a dielectric dispersion of electric polarization near 105 Hz. The effect of electric polarization was also confirmed by charging and discharging current under an application of dc voltage.

기술교과 교육 제도 및 연구의 국제적 동향 : ICTES '95 발표 논문을 중심으로 through Analyzing of Papers Presented at the ICTES '95

이재원,김진순,류창렬,노태천,최완식,최유현,송일민 충남대학교 공업교육연구소 1995 論文集 Vol.18 No.1

The purposes of this study were: (a) to analyze the papers presented at the ICTES according to the papers' representing countries and their main subjects, (b) to analyze the educational systems of the countries in technology education, and (c) to review the international trends of technology education through the papers presented at the conference. The papers(64 total) presented at the ICTES could be classified into eight countries of papers representing Japan(37), Taiwan(8), Korea(6), U. S. A.(3), India(3), Philippines(3), China(2), Singapore(2). The main subjects the papers dealt with could also be classified into educational system/history(9). curriculum/objectives and contents(9), international activities(9), methods/teaching-learning process and teaching materials and new media(40). Followings were the major and new issues presented at the ICTES: (a) Introduction of each country's technology education system. (b) Proposition of a new framework for technology education and a world core curriculum in technology education. (c) Creativity and problem-solving for the instructional method of technology education. (d) Methods and utilization of high technology media in technology education. (e) Necessity of engineering concept in technology education in technology oriented society. (f) Proposition of philosophical direction of technology education based on famous Asian philosophers.

解放 이후 工業高等學校 敎育課程의 變遷過程 硏究

李載元,金振淳,盧泰天,柳彰烈 충남대학교 공업교육연구소 1985 論文集 Vol.8 No.2

The purposes of this study was to : 1) review the curriculum changes in technical high school from the 1945 Liberation to 1984, 2) examine the characteristics, objectives and organizations of the curriculum and, 3) search for the future directions of the curriculum development. Development of technical high school education programs was divided into four periods. The characteristics of THS curriculum during these periods was summarized as follows. 1. Embryonic Period (1945-1962)-After the foundation of the Republic of Korea, the technical high schools were included in high school system according to the Education Law promulgated in 1949. The technical high schools were created to develop competent industrial production workers. General subjects of the technical high schools were same as the general high schools during this period. Specialized subjects accounted for more than 30 percent of the total school hours. 2. Established Period (1963-1972)-Ministry of Education responding to the first Five-Year Plan for economic development, established a new technical high school curriculum in 1963. General course were modified to meet the needs of technical high school students. The educational aim of THS was the training skilled worker and technicians. THS placed stress on specialized subjects which were made-up than 50 percent of total school hours. Also, more hours were spent in practice compared to theory in specialized courses. 3. Developed Period (1973-1979)-Remarkable development was achieved by THS during this period. Technical high schools were subdivided into general, mechanical, special and model technical high schools. Their graduates were eligible for technical qualification and licences after passing test. THS had given the more consideration to the specialized subjects and practice than general subjects and theory. 4. Converting Period (1980-1984)-A change in educational policy in 1980 created a turning point for THS's. The new policy emphasizes educating Industrial Technicians more than training skilled workers. Technical high schools were again included in the high school system. THS placed increased emphasis on general subjects which comprise 43-60 percent of total school hours. The 1981 curriculum revision established elective subjects (e.g., General Mechanics, General Electrics, Computer, Industrial English, Industrial Safety). All THS students are able to select elective subjects outside their own major.

^161, ^163Dy의 0.003eV~50keV의 중성자 포획 단면적 측정

민영기,윤정란,노태익,김귀년,이삼열 東亞大學校附設基礎科學硏究所 2002 基礎科學硏究論文集 Vol.19 No.1

교토대학 원자로 연구소의 46MeV 전자선형가속기를 사용하여 중성자 TOF 방법으로, 에너지 영역 0.003eV∼50keV의 ^161, 163Dy sample의 중성자 포획 단면적을 측정하였다. 본 실험에서 사용된 Bi_4Ge_3O_12(BGO) 섬광 검출기는 sample의 핵반응에서 나오는 즉발 포획 감마선 측정에 사용하였다. 이 검출기는 중성자 선원으로부터 거리 12.7±0.02m 위치에 12개의 블록으로 되어 있으며, sample에 포획되는 중성자 flux의 절대치를 구하기 위해 Sm(n, γ) 및 ^10B(n, αγ) 반응을 이용하였다. ^161, 163Dy에 대한 포획 단면적 측정결과는 ENDF/B-VI 결과와 비교하였다. The neutron capture cross sections of ^161, 162Dy has been measured in the energy region from 0.003eV 50keV by using the neutron time-of-flight(TOF) promptγmethod with a 46MeV electron linear accelerator at the Research Reactor Institute, Kyoto University. An assemble of twelve pieces of BGO scintillation detectors, which was placed at a distance of 12.7±0.02mm from the neutron source, was employed as a total energy absorption detector for the prompt capture gamma-ray measurement, to obtain the absolute capture cross section value. The sample of ^161, 163Dy is a form of metallic plate. An enriched boron sample was employed to monitor the neutron flux/spectrum of the TOF beam using the standard reference cross section of the ^10B(n, αγ) reaction. Previous measurements and evaluated data in ENDF/B-VI was compared with the present results.

Effect of surface-treatments on flexibility and guided bone regeneration of titanium barrier membrane

Kim, Jin-Tae,Kim, Byoung Soo,Jeong, Hee Seok,Heo, Young Ku,Shin, Sang-Wan,Lee, Jeong-Yol,Shim, Young Ho,Lee, Deuk Yong The Korea Association of Crystal Growth 2015 韓國結晶成長學會誌 Vol.25 No.3

Titanium barrier membranes are prepared to investigate the effect of surface-treatments, such as machining, electropolishing, anodizing, and electropolishing + TiN coating, on the biocompatibility and physical properties of the membranes. The surface roughness (Ra) of the membrane decreases from machining ($0.37{\pm}0.09{\mu}m$), TiN coating ($0.22{\pm}0.09{\mu}m$), electropolishing ($0.20{\pm}0.03{\mu}m$), to anodizing ($0.15{\pm}0.03{\mu}m$). The highest ductility (24.50 %) is observed for the electropolished Ti membrane. No evidence of causing cell lysis or toxicity is found for the membranes regardless of the surface-treatments. Cell adhesion results of L-929 and MG-63 show that the machined Ti membrane exhibits the highest cell adhesion while the electropolished membrane is the best membrane for the L-929 cell proliferation after 7 days. However, no appreciable difference in MG-63 cell proliferation among variously surface-treated membranes is detected, suggesting that the electropolished Ti membrane is likely to be the best membrane due to the synergic combination of tailored flexibility and excellent fibroblast proliferation.

[생산기술부문] 사각형 유한요소의 격자 절단과 요소 유연화

김태정(Tae-jeong Kim),김종봉(Jong-bong Kim),양동열(Dong-yol Yang) 한국자동차공학회 1999 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-

In this study, the mesh trimming scheme for bilinear element is introduced. Using finite element method to solve the forming process of the industrial products. the mesh trimming must be implemented. In trimming the bilinear elements. the new points are located on the trimming line. In order to improve the quality of mesh. the points are moved according to three schemes. Furthermore. the properties of nodes and elements should be transformed from the initial mesh to the newly generated mesh. To verify this schems, the mesh of the door panel is trimmed

차량 전장시스템을 위한 OSEK/VDX의 구현

김태열(Kim Tae-Yol),김기웅(Kim Kee-Woong),김재구(Kim Jae-Goo),임홍준(Lim Hong-Joon),류세형(Ryu Syeh-Yung),이석(Lee Suk) 제어로봇시스템학회 2001 제어로봇시스템학회 국제학술대회 논문집 Vol.2001 No.10

Optimization of Reaction Conditions for the Immobilization of a Novel Lipase from Pichia lynferdii NRRL Y-7723 on Hydrophobic Resins

Young-Mi Kim(김영미),Geon-Ho Lee(이건호),In-Hwan Kim(김인환),Ki-Taek Lee(이기택),Tae-Yol Ha(하태열),Ching T. Hou,Hak-Ryul Kim(김학렬) 한국생물공학회 2004 한국생물공학회 학술대회 Vol.2004 No.4