Deletion in HSP110 T₁₇: correlation with wild-type HSP110 expression and prognostic significance in microsatellite-unstable advanced gastric cancers

Kim, K.J.,Lee, T.H.,Kim, J.H.,Cho, N.Y.,Kim, W.H.,Kang, G.H. W. B. Saunders Co ; Centrum Philadelphia 2017 Human pathology Vol.67 No.-

<P>Deletion of the HSP110 T-17 mononucleotide repeat has recently been identified as a prognostic marker that is correlated with wild-type HSP110 (HSP110wt) expression in microsatellite instability-high (MSI-H) colorectal cancers. The aim of this study was to assess the correlation between deletion of the HSP110 T-17 repeat and expression of HSP110wt using DNA testing and immunohistochemistry and to determine the prognostic implications of HSP110 T-17 deletion in MSI-H advanced gastric cancers (GCs). The status of HSP110wt expression was evaluated by immunohistochemistry using an HSP110wt-specific antibody in 142 MSI-H advanced GCs. The size of the HSP110 T-17 repeat deletion was analyzed in 96 MSI-H advanced GCs; deletions were divided into small (0-2 base pairs) and large deletions (3-5 base pairs). Low and high expressions of HSP110wt were detected in 38 (26.8%) and 104 (73.2%) of the 142 cases, respectively. The HSP110 T-17 deletion was observed in 45 (46.9%) of the 96 MSI-H GC samples. Tumors with high expression of HSP110wt showed a tendency to have small or no deletion of HSP110 T-17. In Kaplan-Meier survival analysis, tumors with a large HSP110 T-17 deletion were associated with favorable overall survival and disease-free survival compared with those with small/no deletion of HSP110 T-17. However, HSP110 T-17 deletion size was not an independent prognostic factor in multivariate analysis. In summary, deletion of the HSP110 T-17 repeat was frequently observed in MSI-H GCs, and HSP110 T-17 deletion size was inversely correlated with HSP110wt expression status. Large HSP110 T-17 was not a prognostic indicator in MSI-H GCs. (C) 2017 Elsevier Inc. All rights reserved.</P>

아연 공급원이 반추위내 발효특성과 유생산성 및 이용율에 미치는 영향 - 2. 아연 공급원이 젖소의 반추위내 박테리아수 변화에 미치는 영향 -

정완태(W . T . Chung),신형태(H . T . Shin),이상철(S . C . Lee) 한국영양사료학회 1997 韓國營養飼料學會誌 Vol.21 No.2

A multi-virus detectable microfluidic electrochemical immunosensor for simultaneous detection of H1N1, H5N1, and H7N9 virus using ZnO nanorods for sensitivity enhancement

Han, J.H.,Lee, D.,Chew, C.H.C.,Kim, T.,Pak, J.J. Elsevier Sequoia 2016 Sensors and actuators. B Chemical Vol.228 No.-

This paper describes a multi-detectable and nano-flow immunosensor based on ZnO nanorods (NRs) grown on the inner surface of PDMS sensor region for sensing H1N1, H5N1, and H7N9 influenza viruses simultaneously using electrochemical method. Nanostructured ZnO NRs with a high isoelectric point (IEP ~9.5) tend to interact electrostatically with proteins with lower IEP such as H1N1, H5N1, and H7N9 antibodies. ZnO NRs were hydrothermally grown on the upper inner surface of the nano-flow PDMS sensor region. The forementioned three influenza viruses were successfully detected from three separate sensing regions by measuring the oxidation current of 3,3',5,5'-tetramethylbenzidine (TMB) by horseradish peroxidase (HRP) conjugated on capture antibody of those influenza viruses when proper potential was applied. The proposed immunosensors were evaluated using 1pg/ml, 10pg/ml, 100pg/ml, 1ng/ml, and 10ng/ml of H1N1, H5N1, and H7N9 antigens by amperometry. These immunosensors showed high selectivity toward H1N1, H5N1, and H7N9, which was successfully confirmed by distinguishing the target virus individually from a mixture of three virus antigens. A low limit of detection was demonstrated by detecting as low as 1pg/ml of each virus and it is believed that this was possible by enhancing the sensitivity with the ZnO NRs grown on the PDMS surface in the sensing region. The steady-state oxidation current output linearly increased with respect to the logarithm of the H1N1, H5N1, and H7N9 virus concentrations in the range of 1-10ng/ml.

On the zeolitic imidazolate framework-8 (ZIF-8) membrane for hydrogen separation from simulated biomass-derived syngas

Yin, H.,Lee, T.,Choi, J.,Yip, A.C.K. Elsevier 2016 Microporous and mesoporous materials Vol.233 No.-

<P>Hydrogen separation from biomass-derived syngas is a critical step in the utilization of gasification technology. Compared with the traditional methods, membrane technology provides an effective and low-cost solution for adjusting the gas composition and collecting H-2 in syngas environments. In this study, a zeolitic imidazolate framework-8 (ZIF-8)-based membrane, which is a potential candidate for H-2 separation from biomass-derived syngas, was successfully fabricated through the seeded (secondary) growth method and the subsequent post-treatments. The prepared ZIF-8 membrane exhibited a modest H-2 separation performance for H-2/CO2 and H-2/CO, with separation factors of 4.95 and 6.08, respectively, and a H-2 permeance of 7.81 x 10(-8) mol m(-2) s(-1) Pa-1 at 200 degrees C in the simulated biomass-derived syngas environments (H-2/CO2/CO) with the presence of steam. In particular, the H-2/CO2 and H-2/CO separation factors were increased by 36% and 97%, respectively, with respect to those obtained through bare supports. Despite the promising H2 perm-selectivity, the H-2/CO2 and H-2/CO separation factors of ZIF-8 membranes at 200 degrees C under water-containing syngas environments were maintained up to 10 h but the longer exposure led to the gradual degradation and eventual reduction toward those of bare supports after 15 h seemingly due to the water-involved membrane degradation. This study provides the availability and limitation of ZIF-8 membranes for H-2 separations in stimulated biomass-derived syngas environments. (C) 2015 Elsevier Inc. All rights reserved.</P>

돼지 H-FABP 유전자의 다형성 및 경제 형질과의 연관성 구명

최봉환,김태헌,이지웅,조용민,이혜영,조병욱,정일정 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.5

The purpose of this study was to detect association between genetic variation and economic trait in the porcine heart type fatty acid-binding protein gene as a candidate gene for the traits related with growth and meat quality in pigs. The H-FABP is a 15-kDa protein expressed in several tissues with high demand for fat metabolism such as cardiac and skeletal muscle and lactating mammary gland. H-FABP is small intracellular protein involved in fatty acid transport from the plasma membrane to the site of β-oxidation and/or triacylglycerol or phospholipid synthesis. In this study, H-FABP PCR-RFLP was performed in F_(2) population composed of 214 individuals form an intercross between Korean Native Boars and Landrace sows. PCR products form tow primer sets within H-FABP gene were amplified in 850bp and 700bp. Digestion of PCR products with the restriction digestion enzymes HaeⅢ and Hinf Ⅰ, revealed fragment length polymorphisms(RFL. Ps). The genotype frequencies from H-FABP/HaeⅢ was .29 for genotype DD, .53 for genotype Dd, and .15 for genotype dd, respectively. The genotype frequencies of HH, Hh, and hh from H-FABP(hinf Ⅰ was .38, .41, and .20, respectively, in the population.Relationships between their genotypes and economic traits were estimated. In H-FABP/HaeⅢ locus, there were specific genotypes(Dd and dd) associated with economic traits such as body weight. In H-FABP/Hinf Ⅰ Iocus, Genotypes of HH and Hh associated with growth traits such as body weights at 5, 12, and 30 week of age (p<.05 or p<.001) and back fat thickness, body fat including abdominal and trimmed fat (p<.001) and intramuscular fat(p<.05). The 'H'allele was positivecly associated with gaining of body weight and fatness deposition. In conclusion, a significant association of the H-FABP gene from its genetic variation was found on body weight, intramuscular fat and backfat thickness.

Advanced H2O2 oxidation for diethyl phthalate degradation in treated effluents: effect of nitrate on oxidation and a pilot-scale AOP operation

Ko, K. B.,Park, C. G.,Moon, T. H.,Ahn, Y. H.,Lee, J. K.,Ahn, K. H.,Park, J. H.,Yeom, I. T. IWA Publishing 2008 Water Science & Technology Vol.58 No.5

<P>One of the objectives of this study was to delineate the effect of nitrate on diethyl phthalate (DEP) oxidation by conducting a bench-scale ultraviolet (UV)/H2O2 and O3/H2O2 operations as suggested in a previous study. We also aim to investigate DEP oxidation at various UV doses and H2O2 concentrations by performing a pilot-scale advanced oxidation processes (AOP) system, into which a portion of the effluent from a pilot-scale membrane bioreactor (MBR) plant was pumped. In the bench-scale AOP operation, the O3 oxidation alone as well as the UV irradiation without H2O2 addition could be among the desirable alternatives for the efficient removal of DEP dissolved in aqueous solutions at a low DEP concentration range of 85±15 μg/L. The adverse effect in the UV/H2O2 process was significantly greater than that in the UV oxidation alone, and its oxidation was almost halved by the nitrate. However, the nitrate clearly enhanced the DEP oxidation in the O3 oxidation and O3/H2O2 process. Especially, the addition of nitrate almost doubled the DEP oxidation efficiency in the O3/H2O2 process. The series of pilot-scale AOP operations confirmed that about 30-50% of DEP dissolved in the treated MBR effluent streams was, at least, oxidized by the O3 oxidation alone as well as the UV irradiation without H2O2 addition. The UV photolysis of H2O2 was most effective for DEP degradation with an H2O2 concentration of 40 mg/L at a UV dose of 500 mJ/cm2.</P>

Two-stage partial melting and contrasting cooling history within the Higher Himalayan Crystalline Sequence in the far-eastern Nepal Himalaya

Imayama, T.,Takeshita, T.,Yi, K.,Cho, D.L.,Kitajima, K.,Tsutsumi, Y.,Kayama, M.,Nishido, H.,Okumura, T.,Yagi, K.,Itaya, T.,Sano, Y. Universitetsforlaget ; Elsevier Science Ltd 2012 Lithos Vol.134 No.-

The timing of partial melting and the pressure-temperature (P-T) paths in the High Himalayan Crystalline Sequence (HHCS) in far-eastern Nepal has been investigated using zircon chronology, rare earth element (REE) compositions, and P-T pseudosection analysis. Zircon from migmatites formed during Himalayan thermal events displays inherited magmatic core overgrown by two generations of metamorphic rims. The new rims are distinguished on the basis of their Tertiary ages, low MREE contents, and low Th/U ratios. The inner zircon rims from Sil+Grt+Bt+Kfs+Pl+Qtz and Ky+Sil+Grt+Bt+Ms+Pl+Qtz migmatites at different structural level of the HHCS display ages of c. 33-28Ma (Early Oligocene) and c. 21-18Ma (Early Miocene): these rims are characterized by flat MREE to HREE patterns and were overgrown by partial melt through muscovite dehydration melting under the stability of garnet, which occurred at P=c. 7-10kbar and T=c. 730-780<SUP>o</SUP>C, and at P=c. 8-14kbar and T=c. 720-770<SUP>o</SUP>C, respectively. The outer zircon rims are relatively enriched in HREE with respect to the inner rims and were overgrown at c. 27-23Ma (Late Oligocene) and at c. 18-16Ma (Early Miocene) during melt crystallization accompanying breakdown of garnet at P=c. 4-7kbar and T=c. 650-725<SUP>o</SUP>C. Early Miocene Ms-Bt leucogranites with two successively overgrown zircon rims at c. 18.3+/-0.3Ma and c. 16.3+/-0.2Ma were intruded into Early Oligocene migmatite hosts. Microstructural observations and the corresponding P-T conditions associated with the two generations of zircon rims indicate that the Early Oligocene and Early Miocene migmatites show relatively isobaric and nearly isothermal P-T paths during exhumation, respectively. The inferences are consistent with higher average cooling rates for the Early Miocene (c. 30-40<SUP>o</SUP>C/My) than the Early Oligocene (c. 15-25<SUP>o</SUP>C/My) migmatites, inferred from peak-T conditions and FT (c. 6Ma for both migmatites) and U-Pb zircon ages. The P-T-t paths of the two migmatites indicate that burial of the Early Miocene migmatites has been coeval with exhumation of the Early Oligocene migmatites, implying the formation of large-scale thrust within the HHCS.

Conventional and inverse magnetocaloric effects, and critical behaviors in Ni₄₃Mn₄₆Sn₈In₃ alloy

Thanh, T.D.,Nan, W.Z.,Nam, G.,Van, H.T.,You, T.S.,Phan, T.L.,Yu, S.C. Elsevier 2015 CURRENT APPLIED PHYSICS Vol.15 No.10

A systematic study of the conventional and inverse magnetocaloric effects, and critical behaviors in an alloy ingot of Ni<SUB>43</SUB>Mn<SUB>46</SUB>Sn<SUB>8</SUB>In<SUB>3</SUB> has been performed. Our results reveal the sample exhibiting structural and magnetic phase transitions at temperatures T<SUB>C</SUB><SUP>M</SUP> = 166 K (T<SUB>C</SUB> of the martensitic phase), T<SUB>M-A</SUB> = 260 K (the martensitic-to-austenitic phase transformation) and T<SUB>C</SUB><SUP>A</SUP> = 296 K (T<SUB>C</SUB> of the austenitic phase). The large values of refrigerant capacity (RC) around T<SUB>M-A</SUB> and T<SUB>C</SUB><SUP>A</SUP> are found to be RC<SUB>M-A</SUB> = 172.6 and RC<SUB>A</SUB> = 155.9 J kg<SUP>-1</SUP>, respectively, under an applied field change of 30 kOe. Our critical analyses near the T<SUB>C</SUB><SUP>M</SUP> and T<SUB>C</SUB><SUP>A</SUP> reveal that a coexistence of the long- and short-range ferromagnetic order in the martensitic phase, while the long-range ferromagnetic order exists in the austenitic phase. Interestingly, at around T<SUB>C</SUB><SUP>A</SUP>, the maximum magnetic entropy change (|ΔS<SUB>max</SUB>|) versus magnetic field H obeys a power law, |ΔS<SUB>max</SUB>| = a.H<SUP>n</SUP>, where the exponent n is found to be about 0.66.

Bacterial community analyses by pyrosequencing in dark fermentative H₂-producing reactor using organic wastes as a feedstock

Im, W.T.,Kim, D.H.,Kim, K.H.,Kim, M.S. Pergamon Press ; Elsevier Science Ltd 2012 INTERNATIONAL JOURNAL OF HYDROGEN ENERGY - Vol.37 No.10

Hydrogen(H<SUB>2</SUB>)-producing bacterial community structures of the dark fermentation system in a batch reactor were investigated during 48 h by analyzing 16S rRNA gene sequences obtained from pyrosequencing. Organic wastes composed of food waste and sewage sludge were used as a feedstock. After heat treatment (90 <SUP>o</SUP>C for 20 min) of the feedstock, H<SUB>2</SUB> was naturally evolved under anaerobic mesophilic conditions, showing a H<SUB>2</SUB> yield of 2.26 mol H<SUB>2</SUB>/mol hexose<SUB>added</SUB>. The bacterial community structure of the initial inoculum (microbial community at the starting point (0 h)) combined with heat treated food waste and sewage sludge was mainly comprised of Proteobacteria and Bacteroidetes. After 6 h operation, the sequences that belong to other groups except Firmicutes decreased dramatically and were not observed at all in the latter samples. Clostridium spp., which were negligible in the inoculum, took over the main bacterial community by taking charge of H<SUB>2</SUB> production. Among the phylum Firmicutes, the sequences closely related with Clostridium sordellii ATCC 9714<SUP>T</SUP>, Clostridium perfringens ATCC 13124<SUP>T</SUP>, and Clostridium butyricum ATCC 19398<SUP>T</SUP> became predominant in the time series within 48 h. Overall, the results showed how fast the Clostridium spp. overwhelmed the bacterial community in dark fermentative H<SUB>2</SUB> production conditions, where they were at a negligible amount at the start.

고단백질 사료 급여시 사료내 에너지 , 단백질 수준과 사료의 가공방법이 이스라엘 잉어의 성장 , 소화율 및 오염부하량에 미치는 영향

원태희(T . H . Won),한인규(I . K . Han),주광석(K . S . Chu) 한국영양사료학회 1994 韓國營養飼料學會誌 Vol.18 No.6