연두금파리(Lucillia illustris)의 용과 성체에서 정소의 미세구조변화와 정자형성과정

김우갑,김지현,이정원,강성훈 고려대학교 한국곤충연구소 1994 昆蟲硏究誌 Vol.20 No.1

연두금파리(Lucillia illustris)의 용과 성체에서 정소의 미세구조변화와 정자형성과정. 이정원, 강성훈, 김지현, 김우갑 (고려대학교 이과대학 생물학과) 昆蟲硏究誌. 20 : 1~13, 1994. 용 3일째부터 성충우화 후 5일까지 연두금파리(Lucilia illustris)의 정소를 적출하여 변태과정에서 정소의 미세구조변화와 정자형성과정에 대하여 광학 및 투과전자현미경으로 관찰하여 다음과 같은 결과를 얻었다. 웅성내부생식기는 한쌍의 정소, 수정관, 한쌍의 부속선, 사정관으로 구성되어 있으며, 각 정소는 하나의 타원형인 정소여포를 가지고 있었다. 같은 발생단계의 세포들은 정원세포다발, 정모세포다발, 정세포다발, 정자다발들을 이루고 있었다. 정소여포는 두층의 피초(sheath)로 둘러싸여져 있었다. 외초(outer sheath)에는 전자밀도가 높은 색소과립이 존재하였고, 내초(inner sheath)에는 미세섬유(microfibril)가 관찰되었으며, 성충이 되면서 색소과립의 수가 증가하고, 미세섬유도 매우 조밀하게 나타났다. 세포질교는 정원세포에서 정세포 시기까지 있어 한 피낭내에 세포들은 같은 분화단계를 나타내었다. 미토콘드리아는 초기 정세포에서 부핵(nebenkern)을 형성하고, 이들이 분열하여 크기가 같은 2개의 미토콘드리아 유도체로 신장하였다. 정세포가 신장하는 동안 핵에서는 바깥쪽에서부터 안쪽으로 염색체들이 점점 응축되었다. 미세소관은 핵의 신장시기에 많은 수로 형성되어 정세포의 신장을 유도하였다. 축사의 미세소관의 배열은 9+9+2이며, 성숙정자의 미부에 2개의 미토콘드리아 유도체사이에 전자밀도가 높은 막대모양의 물질이 일정하게 배열되어 있는 특징적인 구조를 나타내었다. We investigated the line structural changes and spermatogenesis of the tests from Lucilia illustris, using light microscope and transmission electron microscope. The male internal reproductive nigan consists vas defference a pair of nceessory glands, and a ejaculatory duct. Each testis is ceraposed of one follicle. Within the testes of different developmental stages, the cells at simllar developmental stage aggregate to form sperinatogonia bunele, sperinatocyte bundle, spermatid bundle, and sperm bundle which are embraced by a cyst. The layer of a testicular follicle consists of two sheath cell layers, outer sheath cell layer and inner sheath cell layer. The outer sheath cell layer contains electron-dense pigment granules, while the inner sheath cell layer is filled with microfibrlis. the granules in outer sheath cell layer and mierofibrils in inner sheath cell layer are richer in adult tests than in pual testis. All the cells in a cyst connect with each other by cytoplasmic bridges during spermatogenesis. The interconnection is responsible for the synchrony of differentiation in cyst. In early spermatid, mitochondria change into nebenkern. The nebenkern then divides into two mitochondrial derivatives. The paired mitochondrial derivatives eventually elongate around the axial fllaments. The chromosomes in nucleus appear to be more condensed while spermatid elongates. The chromosome condensation initiated from the outside within nucleus gradually progresses into the inside. Numerous microtubles which occur during nuclear elongation induce elongation of the spermatid. The middle piece of a sperm consists of the axon eme with 9+9+2 microtublar pattern and two same-sized mitochondrial derivatives between which the electron dense materials are located.

배양된 혈관내피세포에서 uncoupling protein 2 유전자 과발현이 세포내 산화스트레스 및 endothelin - 1 mRNA 발현에 미치는 영향

강효경,한정희,홍성관,김하영,남궁일성,박기영,박혜선,이재담,박중열,이인규,이기업 한국지질학회 2001 韓國脂質學會誌 Vol.11 No.2

당뇨병유발백서에서 급성패혈증 병발시 적혈구막지질과산화의 변화

박성우,홍성관,유형준,장대영,강승식,유재명,임성희,최문기,노정우,이규만 대한당뇨병학회 1994 Diabetes and Metabolism Journal Vol.18 No.3

급성 골수성 백혈병(M6)에 합병된 중추성 요붕증 1 예

김성연,조보연,이홍규,고창순,민헌기,김병국,김노경,신찬수,이명식,강덕현,조종태 대한내과학회 1989 대한내과학회지 Vol.37 No.2

Leukemia is known to be an extremely rare cause of diabetes insipidus. We report a case of diabetes insipidus as a complication of leukemia. An 18 year-old man was admitted because of polyuria polydipsia and fever. Bone marrow aspiration and biopsy revealed the diagnosis of acute erythroleukemia (AML, M6). On the water deprivation test, the diabetes insipidus was confirmed to be of the central type. He was managed with intranasal vasopressin for diabetes insipidus with good response. Induction chemotherapy was administered without response, and the patient died of septicemia and respiratory failure 2 months after initial diagnosis. A short review of the literature related to this subject is also presented.

방전 플라스마에 의한 CFC-12(CCl2F2)의 분해

황명환,우인성,조정국,강현춘,안형환,이한섭,강안수 한국산업안전학회 1999 한국안전학회지 Vol.14 No.3

Decomposition efficiency, power consumption, and applied voltage of CFC(Chlorofluorocarbon) were investigated by SPCY(surface induced discharge plasma chemical processing) reactor to obtain optimum process variables and maximum decomposition efficiencies. Decomposition efficiency of CFC-12 with various electric frequencies(5∼50㎑). flow rates (100∼1,000mL/min), initial concentrations(100∼1,000ppm), electrode materials(W, Cu. Al), electrode thickness(1, 2, 3mm) and reference gases(N₂, O₂, air) were measured and the products were analyzed with FT-IR. Experimental results showed that at the frequency of 10kHz, the highest decomposition efficiency of 92.7% for CFC-12 were observed at the power consumptions of 29.6W, respectively, and that decomposition efficiency decreased with increasing frequency above 20㎑ and decomposition efficiency per unit power were 3.13%/W for CFC-12. Decomposition efficiency was increased with increasing residence times and with decreasing initial concentration of pollutants. Decomposition efficiency was increased with increasing thickness of discharge electrode and the highest decomposition efficiency was obtained for the electrode diameter of 3mm. As the electrode material, decomposition efficiency was in order that tungsten(W), copper(Cu), aluminum (Al).

제 2 형 당뇨병에서 미세혈관 합병증과 대혈관 합병증의 대비

강문호,이기영,정연실,김홍규,서찬종,송광식,박혜영,이원기 대한당뇨병학회 2001 Diabetes and Metabolism Journal Vol.25 No.2

Background: Type 2 diabetes is a heterogeneous disease. As to its complications, microangiopathy predominantly develop in some patients while macroangiopathy is more predominant in others. Therefore, this study was performed to identify the factors associated with such dissociation. Methods: Type 2 diabetic patients were classified into the macro and microangiopathy groups by carotid intima-medial thickness (IMT) and the presence of severe diabetic retinopathy. Patients with IMT ≤ 0.83 mm and severe NPDR or PDR were assigned to microangiopathy group; those with IMT 0.84 mm and no diabetic retinopathy were assigned to macroangiopathy group. Of 95 patients studied, 14 were classified as microangiopathy group and 20 as macroangiopathy group. Results: When the microangiopathy and macroangiopathy groups were compared, the microangiopathy group were significantly younger at the time of examination and at the onset of diabetes, and had longer duration of diabetes. Percentage of patients receiving insulin treatment was significantly higher and plasma C-peptide significantly lower in the microangiopathy group. However, HDL-cholesterol levels were significantly lower in the macroangiopathy group. Furthermore, the multiple logistic regression analyses revealed that younger onset of type 2 diabetes was an independent factor that was associated with the acceleration of microangiopathy. Conclusion: This study suggests that the age at onset of diabetes is an important independent risk factor that is associated with predominant development of microangiopathy in type 2 diabetic patients.

단조용 환봉이 유도가열실험 및 이론 해석에 관한 연구

최진호,이원재,강성욱,주성호,조봉환,김병화 대한금속재료학회(대한금속학회) 1999 대한금속·재료학회지 Vol.37 No.12

A solid type crankshaft, one of the most important part of medium size marine engine, is manufactured by forging method. The induction heating system(600 ㎾ = 100%, 190∼200 ㎐) is used to heat rounded bar(Ø285 ㎜) up to 1200℃ for the forging. Numerical analysis was performed on the heating process at various operating conditions by applying boundary conditions based on theoretical approach. Experiments were also performed on the same type of mended bar to confirm the simulation results. Experimental results showed that the induction heating phenomena were effected by the phase transformation at A_(c₁) A_(c₃) temperature range, the magnetic transformation at A₂ (curie point) temperature range and the mass effect of large volume of rounded bar. At the 62% power, our induction heating system showed the maximum efficiency and the heating time was about 40∼50 minutes.

신장의 이상회전과 T2Hemivertebra 를 동반한 선천성 일측폐 발육 부전증 1 예

성차경,배성조,강민구,허경억,이대규,도사금 대한내과학회 1986 대한내과학회지 Vol.31 No.2

A case of congenital agenesis of left lung of a 19-years-old female is presented in detail with some review of literature. She had been suffered from mild dyspnea on exertion since early childhood but she was been comfatable unti16 months ago, when frequent upper respiratory infection and dyspnea developed. From 10 days earlier, mild fever was associated and dyspnea on exertion was aggravated. Her past history and family history were not contributary. Physical examination revealed asymmetrical development of both thoracic cage and showed limited respiratory movement of left thorax. There were increased percussion dullness and absent breathing sounds over entire left lung field except left upper lung field. The cardiac dullness was shifted to the left and heart sunds were heard over the 4th ICS X MCL. A roentgenogram of the chest revealed shifting to the left of trachea and mediastinum, homosgenou increased density of left lung, hyperlucency of right lung, and T, hemivertebra, Bronchoscopy showed total absence of left main bronchus. Bronchogram revealed above findings too, but right main bronchus was intact. CT scan revealed agenesis of left lung and herniation of right lung to left hemithorax. Intravenous pyelography showed malrotation of kidney. Pulmonary angioram and cardiac catheterization showed unremarkable findings except total absence of left pulmonary artery.

초내열합금 Mar-M 247 LC 에서 열처리에 의한 미세조직의 변화와 크리프특성과의 관계

김일호,최병학,강수현,조종춘,허보영,김학매 대한금속재료학회(대한금속학회) 1999 대한금속·재료학회지 Vol.37 No.3

Creep-rupture properties of a cast type Superalloy, Mar-M247LC. were studied in 760℃ and 980℃. The shape and size of γ′ particles in grains were known to have significant influence on the creep behavior and rupture life in relation to the micro-mechanism of plastic deformation. It was found that the creep properties were also affected by the cast structure which is composed of dendrite matrix and eutectic boundaries. The hardening effect of boundaries by solute segregation and eutectic phases may improve the creep resistance of grain or eutectic boundaries during creep-rupture.

폭발합성법에 의한 Ti5Si3 계 금속간화합물의 합성

강봉용,황선근,류회수,김상우 대한금속재료학회(대한금속학회) 1998 대한금속·재료학회지 Vol.36 No.10

Synthesis of an intermetallic compound based on Ti_5Si₃ by an explosive compaction of elemental powders was studied. The main experimental variable was the detonation velocity, and it was found that the relative density of the compacted compound increased with the detonation velocity. To enhance the density of the compound further, however, it was deemed necessary to consider variables such as the can, powder and the backup tube. From an X-ray diffraction analysis of the explosion-compacted compound it was confirmed that formation of the Ti_5Si₃ phase was complete. Apart from the needs to further improve the density and crack resistance of the compacted alloy, the present work indicates that the explosion synthesis has a potential to be developed as a consolidation method for Ti_5Si₃ intermetallic compounds.