Isolation of the Threonine Dehydratase Gene from a Tylosin - Producing Strain of Streptomyces fradiae

LEE,SANG HEE,KYE JOON LEE 한국미생물생명공학회 1995 Journal of microbiology and biotechnology Vol.5 No.5

수답에서 2-(4-(6-chloro-2-benz-oxazolyloxy)phenoxy)-N-phenylpropionamide 유도체 중 N-phenyl 치환체들의 제초활성

성낙도(Nack-Do Sung),이상호(Sang-Ho Lee),고영관(Young-Kwan Ko),이경모(Kyung-Mo Lee),김대황(Dae Whang Kim),김태준(Tae-Joon Kim) 한국농약과학회 2000 농약과학회지 Vol.4 No.2

A new fourty six 2-(4-(6-chloro-2-benzoxazolyloxy)phenoxy)-N-phenylpro- pionamide derivatives were synthesized and the herbicidal activities against rice plant and barnyard grass with pre-emergence in down land were measured. The structure activity relationships (SAR) between the activities and physicochemical parameters of the substituted(X) N-phenyl group in substrates were analyzed and discussed by Free- Wilson and Hansch method from the basis on the former study (Sung. et. al., 1999). The conditions of selective herbicide activity both the barnyard grass and rice plant are shown that the optimal bydrophobicity, (π)<SUB>opt</SUB>.=1.34 and electron donating with field effect (F<0) of meta and ortho, para-substituted mono or disubstituent on the N-phenyl ring were found to contribute significantly. The herbicidal activities against barnyard grass are roughly the same as the results in up land whereas damage to rice plant in down land more increase than that of up land. Degradation products in water are 2-(4-6-chloro-2-benzoxazolyloxy)phenoxy)propionic acid ((A)) (obs. pKa=4.35 & obs. logP=4.77) and 6-chloro-2-benzoxazolone (B) (obs. pKa=8.40 & obs. logP=2.90). These results were supposing that the hydrolysis product of substrates, (A) is comparatively absorbed in rice plant but not in barnyard grass. And it is assumed from the SAR equations that the 2,6-dimethyl-4-methoxymethyl group substituent (pI??=5.41, 3g/㏊) is selected as the most highest herbicidal acitvity against barngard grass in green house.

Safety of subtotal or total colectomy with primary anastomosis compared to Hartmann procedure for left-sided colon cancer obstruction or perforation

Eun-Do Kim,Jin-Kwon Lee,Jin-Kyu Cho,Jae-Myung Kim,Ji-Ho Park,Ju-Yeon Kim,Sang-Ho Jeong,Young-Tae Ju,Chi-Young Jeong,Eun-Jung Jung,Young-Joon Lee1,Soon-Chan Hong,곽승진 대한종양외과학회 2019 Korean Journal of Clinical Oncology Vol.15 No.2

Purpose: Whether subtotal or total colectomy with primary anastomosis (PA) is safer than Hartmann procedure (HP) for left-sided colon cancer obstruction or perforation remains controversial. The purpose of this study was to compare postoperative morbidity, mortality, and defecation frequency between PA and HP for left-sided colon cancer obstruction or perforation. Methods: This retrospective study enrolled 54 patients from January 2014 to February 2018 who underwent emergency surgery due to left-sided colon cancer obstruction or perforation. Results: PA was carried out in 20 patients while HP was performed for 34 patients. Thirty-day mortality did not show significant difference between the two groups (15.0% vs. 14.7%, P=1.000). No anastomotic leakage occurred in PA group while three (8.8%) cases of stump leakage occurred in HP group. Stoma repair was performed for 13 cases (44.8%) and stoma reformation was performed for one case in HP group (7.7%). Stoma related complications occurred in five cases (17.24%). For patients after stoma repair, defecation frequency at 3 months after operation was 2.91±2.88 times per day in PA group and 2.86±2.63 times per day in HP group. At 1 year after operation, defecation frequency was changed to 1.40±1.12 times per day in PA group and 1.17±0.39 times per day in HP group. Conclusion: Primary ileosigmoid or ileorectal anastomosis for left-sided colon cancer obstruction or perforation is safe, and shows similar outcome of defecation frequency compared to H

Erratum : Quercetin Inhibits the 5-Hydroxytryptamine Type 3 Receptormediated Ion Current by Interacting with Pre-Transmembrane Domain I

Lee, Byung-Hwan,Jeong, Sang-Min,Lee, Jun-Ho,Kim, Jong-Hoon,Yoon, In-Soo,Lee, Joon-Hee,Choi, Sun-Hye,Lee, Sang-Mok,Chang, Choon-Gon,Kim, Hyung-Chun,Han, YeSun,Paik, Hyun-Dong,Kim, Yangmee,Nah, Seung-Ye Korean Society for Molecular and Cellular Biology 2005 Molecules and cells Vol.20 No.3

Short and Long-term Outcomes of Subarachnoid Hemorrhage Treatment according to Hospital Volume in Korea: a Nationwide Multicenter Registry

Lee Ji Young,Heo Nam-Hun,Lee Man Ryul,Ahn Jae Min,Oh Hyuk-Jin,Shim Jai Joon,Yoon Seok Mann,Lee Bo Yeon,Shin Ji Hyeon,Oh Jae Sang 대한의학회 2021 Journal of Korean medical science Vol.36 No.22

Background: Subarachnoid hemorrhage is a potentially devastating cerebrovascular attack with a high proportion of poor outcomes and mortality. Recent studies have reported decreased mortality with the improvement in devices and techniques for treating ruptured aneurysms and neurocritical care. This study investigated the relationship between hospital volume and shortand long-term mortality in patients treated with subarachnoid hemorrhage. Methods: We selected subarachnoid hemorrhage patients treated with clipping and coiling from March–May 2013 to June–August 2014 using data from Acute Stroke Registry, and the selected subarachnoid hemorrhage (SAH) patients were tracked in connection with data of Health Insurance Review and Assessment Service to evaluate the short-term and long-term mortality. Results: A total of 625 subarachnoid hemorrhage patients were admitted to high-volume hospitals (n = 355, 57%) and low-volume hospitals (n = 270, 43%) for six months. The mortality of SAH patients treated with clipping and coiling was 12.3%, 20.2%, 21.4%, and 24.3% at 14 days, three months, one year, and five years, respectively. The short-term and long-term mortality in high-volume hospitals was significantly lower than that in low-volume hospitals. On Cox regression analysis of death in patients with severe clinical status, lowvolume hospitals had significantly higher mortality than high-volume hospitals during shortterm follow-up. On Cox regression analysis in the mild clinical status group, there was no statistical difference between high-volume hospitals and low-volume hospitals. Conclusion: In subarachnoid hemorrhage patients treated with clipping and coiling, lowvolume hospitals had higher short-term mortality than high-volume hospitals. These results from a nationwide database imply that acute SAH should be treated by a skilled neurosurgeon with adequate facilities in a high-volume hospital.

Expression of Neutrophil Gelatinase-Associated Lipocalin in Calcium-Induced Keratinocyte Differentiation

Lee, Jeung-Hoon,Kye, Kyung-Chae,Seo, Eun-Young,Lee, Kyungmoon,Lee, Sang-Keun,Lim, Jong-Soon,Seo, Young-Joon,Kim, Chang Deok,Park, Jang-Kyu The Korean Academy of Medical Sciences 2008 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.23 No.2

<P>In a previous search for the differentially expressed genes in keratinocyte differentiation, we identified neutrophil gelatinase-associated lipocalin (NGAL) as a calcium-induced gene. In this study, we further verified the expression of NGAL in cultured keratinocytes as well as in several skin diseases. Reverse transcription-polymerase chain reaction (RT-PCR), Western blot, and ELISA clearly showed that NGAL expression was markedly increased in calcium-induced keratinocyte differentiation in vitro. However, in our previous report, NGAL expression was not detected in normal skin tissue except for hair follicle by in situ hybridization and immunohistochemistry, indicating the difference of cell status between in vitro and in vitro conditions. Interestingly, NGAL expression was highly increased in psoriasis-like inflammatory disorders (lichen planus and pityriasis rubura pilaris) and skin cancers (keratoacanthoma and squamous cell carcinoma), implying that NGAL may be related with the epidermal hyperplasia. Collectively, these results reveal the potential importance of NGAL in the maintenance of skin homeostasis.</P>

Three‐Dimensional Self‐Assembly of Graphene Oxide Platelets into Mechanically Flexible Macroporous Carbon Films

Lee, Sun Hwa,Kim, Hyun Wook,Hwang, Jin Ok,Lee, Won Jun,Kwon, Joon,Bielawski, Christopher W.,Ruoff, Rodney S.,Kim, Sang Ouk WILEY‐VCH Verlag 2010 Angewandte Chemie Vol.122 No.52

<P><B>Graphenoxid‐Plättchen</B> können sich zu hoch geordneten und flexiblen Kohlenstoff‐Filmen mit einstellbaren Porenmorphologien organisieren. Durch Dotieren mit Stickstoff werden die elektrischen Eigenschaften und Superkondensator‐Charakteristika der kohlenstoffbasierten Strukturen verbessert, und eine chemische Funktionalisierung wird ermöglicht.</P>

Size and Strain Analysis of Deformation Microstructure in High-Nitrogen Austenitic Stainless Steels

Lee, Tae Ho,Shin, Eun Joo,Ha, Heon Young,Oh, Chang Seok,Kim, Sung Joon Trans Tech Publications, Ltd. 2010 Materials science forum Vol.652 No.-

<P>Deformation microstructure of Fe-18Cr-10Mn alloys with different nitrogen content (0.39 and 0.69N) were investigated using neutron diffraction. The deformation mode changed from strain-induced martensitic transformation (SIMT) to deformation twinning (DT), as nitrogen content was increased. The measured neutron profiles of tensile-strained bulk samples were fitted by modified Thomson-Cox-Hasting profile-shape function and the Lorentzian and Gaussian integral breadths were calculated based on Rietveld refinement. With the calculated integral breadths, the mean-squared (MS) strain could be evaluated using the size and strain analysis of double-Voigt approach. Based on the analysis of peak shift of individual reflections, the stacking fault probability (SFP) was determined. Although the calculated SFP and MS strain varied with the degree of deformation, the ratio of MS strain to SFP remained constant for each alloys regardless of tensile strain, but increased with nitrogen content. As a result, it was found that the SFE increased with increasing nitrogen content. The nitrogen-induced transition of deformation microstructure was accounted for by the change in SFE, as reported in other fcc materials. </P>

Activation of human monocytes by a formyl peptide receptor 2-derived pepducin

Lee, Ha Young,Kim, Sang Doo,Shim, Jae Woong,Kim, Hak Jung,Kwon, Jae Young,Kim, Jong-Min,Baek, Suk-Hwan,Park, Joon Seong,Bae, Yoe-Sik Elsevier 2010 FEBS letters Vol.584 No.18

<P><B>Abstract</B></P><P>We synthesized and investigated the effect of formyl peptide receptor 2 (FPR2)-derived pepducins in human monocytes. The FPR2-based cell-penetrating lipopeptide, “pepducin” (F2pal-16), stimulated intracellular calcium increase in human monocytes via pertussis toxin (PTX)-sensitive G-protein and phospholipase C (PLC) activity. From a functional aspect, we showed that F2pal-16 stimulated monocyte chemotaxis. F2pal-16 also stimulated the generation of superoxide anion in human monocytes. Moreover, F2pal-16 dramatically increased the production of several kinds of pro-inflammatory cytokines (CXCL8, CCL2, IL-1β and TNF-α) in human monocytes via NF-κB activation. Since FPR2 plays an important role in immune responses, F2pal-16 can serve as a useful reagent for the study of FPR2-mediated immune modulation.</P>

Measurement of red blood cell aggregation using X-ray phase contrast imaging.

Lee, Sang Joon,Ha, Hojin,Nam, Kweon-Ho Optical Society of America 2010 Optics express Vol.18 No.25

<P>When a coherent beam illuminates spatially disordered particles, speckle patterns are formed due to interference of the scattered light waves. Speckle patterns from biological tissues using synchrotron phase contrast X-ray imaging can provide functional information about micro-scale morphological structures of the tissues. In this study, we investigated the size and contrast variations of the speckles of aggregated red blood cells (RBCs) suspensions with varying the degree of RBC aggregation. Results show that the degree of RBC aggregation is a governing parameter on the change of speckle characteristics. This blood speckle analysis method can be used as a novel modality for monitoring RBC aggregation.</P>