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Yumnam, Silvia,Raha, Suchismita,Kim, Seong Min,Saralamma, Venu Venkatarame Gowda,Lee, Ho Jeong,Ha, Sang Eun,Heo, Jeong Doo,Lee, Sang Joon,Kim, Eun Hee,Lee, Won Sup,Kim, Jin A.,Kim, Gon Sup D.A. Spandidos 2018 ONCOLOGY REPORTS Vol.40 No.6
<P>Proteomic analysis serves as an important biological tool for identifying biological events. Novel biomarkers of a specific disease such as cancer may be identified using these promising techniques. The aim of the present study was to investigate the effect of tangeretin and to identify potential biomarkers in AGS gastric cancer cells using a proteomics approach. The results of the present study revealed that tangeretin inhibited AGS cell viability dose-dependently with a half-maximal inhibitory concentration of 100 µM. Two-dimensional gel electrophoresis was performed to determine the potential biomarker between control and tangeretin (100 µM)-treated AGS cells. A total of 16 proteins was identified from 36 significant protein spots using matrix-assisted laser-desorption/ionization time-of-flight-mass spectrometry using peptide fingerprinting. The bioinformatics tools Protein ANalysis THrough Evolutionary Relationships (PANTHER) and Database for Annotation, Visualization and Integrated Discovery (DAVID) were used to identify the functional properties and association of the proteins obtained. Using western blot analysis, the regulatory pattern of four selected proteins, protein kinase Cε, mitogen-activated protein kinase 4, phosphoinositide 4-kinase and poly(ADP-ribose) polymerase 14, were successfully verified in replicate sample sets. These selected proteins are primarily involved in apoptosis signaling, angiogenesis, cell cycle regulation, receptor kinase binding, intracellular cytoplasmic and nuclear alterations. Therefore, aim of the present study was to identify potential diagnostic biomarkers from the functional categories of altered protein expression in tangeretin-inhibited AGS gastric cancer cell viability.</P>
Scutellarein Induced G2/M Cell Cycle Arrest and Apoptosis in Hep3B Hepatocellular Carcinoma Cells
Sang Eun Ha,Ho Jeong Lee,Suchismita Raha,Venu Venkatarame Gowda Saralamma,Seong Min Kim,Anjugam Paramanantham,Jeong Doo Heo,Sang Joon Lee,Eun Hee Kim,Gon Sup Kim 대한수의학회 2017 대한수의학회 학술대회발표집 Vol.2017 No.-
Proteomic profiling of human HepG2 cells treated with hesperidin using antibody array
Yumnam, Silvia,Saralamma, Venu Venkatarame Gowda,Raha, Suchismita,Lee, Ho Jeong,Lee, Won Sup,Kim, Eun Hee,Lee, Sang Joon,Heo, Jeong Doo,Kim, Gon Sup SPANDIDOS PUBLICATIONS 2017 MOLECULAR MEDICINE REPORTS Vol. No.
<P>Protein array technology not only identifies a large number of proteins but also determines their expression levels. In the present study, antibody array analysis is used to decipher the proteins involved in hesperidin-induced cell death in HepG2 cells. Altered proteins in hesperidin treated cells were compared with that of untreated control cells by using a RayBio<SUP>®</SUP> Label-based (L series) human antibody array kit. The identified proteins were further confirmed using western blot analysis. STRING software based analysis was used to determine the protein-protein interactions. Many proteins related to signal transduction, cellular mechanisms, cell growth and proliferation regulatory proteins were identified. Among the proteins identified Hsp90, Smac/DIABLO, Prdx6 and FRK were significantly reduced in hesperidin treated cells. To the best of the authors' knowledge, the present study is the first to use antibody array for identifying proteins marker in hesperidin-induced cell death in HepG2 cells. The present study provides a novel insight into the anticancer mechanism of hesperidin.</P>