Inhibition of oxidative stress induced‑cytotoxicity by coptisine in V79‑4 Chinese hamster lung fibroblasts through the induction of Nrf‑2 mediated HO‑1 expression

Hyeon‑Gyun Jo,Cheol Park,이혜숙,Gi‑Young Kim,Young‑Sam Keum,Jin Won Hyun,Taeg Kyu Kwon,Yung Hyun Choi,Su Hyun Hong 한국유전학회 2021 Genes & Genomics Vol.43 No.1

Background Coptisine is a natural alkaloid compound and is known to have multiple benefcial efects including antioxidant activity. However, whether it can protect lung fbroblasts from oxidative damage has not been studied yet. Objectives To investigate the potential inhibitory efect of coptisine against oxidative stress in V79-4 lung fbroblast cells. Methods V79-4 cells were treated with H2O2 (1 mM) in the presence or absence of coptisine (50 µg/ml), N-acetyl cysteine (NAC, 10 mM) or zinc protoporphyrin IX (ZnPP, 10 µM) for the indicated times. The alleviating efects of coptisine on cytotoxicity, cell cycle arrest, apoptosis, reactive oxygen species (ROS) production, DNA damage, mitochondrial dynamics, and inhibition of ATP production against H2O2 were investigated. Western blot analysis was used to analyze the expression levels of specifc proteins. Results Coptisine inhibited H2O2-induced cytotoxicity and DNA damage by blocking abnormal ROS generation. H2O2 treatment caused cell cycle arrest at the G2/M phase accompanied by increased expression of cyclin-dependent kinase (Cdk) inhibitor p21WAF1/CIP1 and decreased expression of cyclin B1 and cyclin A. However, these efects were attenuated in the presence of coptisine or NAC. Coptisine also prevented apoptosis by decreasing the rate of Bax/Bcl-2 expression in H2O2-stimulated cells and suppressing the loss of mitochondrial membrane potential and the cytosolic release of cytochrome c. In addition, the activation of nuclear factor-erythroid-2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) was markedly promoted by coptisine in the presence of H2O2. However, zinc protoporphyrin IX, a potent inhibitor of HO-1, attenuated the ROS scavenging and anti-apoptotic efects of coptisine. Conclusions Based on current data, we suggest that coptisine can be used as a potential treatment for oxidative stress-related lung disease.

한국 근대문학 연구의 새 지평 ; 근대시 연구의 새 지평 ―전통주의적 연구를 중심으로-

박현수 ( Hyeon Su Park ) 현대문학이론학회 2008 現代文學理論硏究 Vol.0 No.33

The purpose of this paper is to investigate the notion and present state of traditionalist research in the study of Korean poetry. In this paper, traditionalist research means a study which to search out new notions and paradigms of discourse in Korean traditional thought and to apply to the study of modern Korean poetry by creative interpretation of those. Closely looking into traditionalist studies, there are five categories; general conceptual, Buddhist, Taoist, neo-Confucianist, Korean fundamental philosophic study. General conceptual study, based on traditional philosophic concepts in the history of Korean thought, was conducted by Yun Jae-geun, Kim Young-seok and Choi Seung-ho. Buddhist study, by Song Uk, Taoist study, by Lee Seonghee and Kim Kyoung-bog, neo-Confucianist study, by Jo Dong-il and Park Hyeon-su, and Korean fundamental philosophic study, by Seo Jeong-ju, Shin Beom-sun and Kim Ji-ha. Though these studies, in the making process, had some blurs in logical refinement, they have very important significance in Korean literary study in that, they tried to find a new and creative theory. It is also worthy to note that traditionalist studies brought a change in the study of Korean literature subordinated every discourse of Korean literature to the western theory.

Fabrication and evaluation of SU-8 based wireless pressure sensor

Gang-Hyeon Sun(선강현),Dong-Su Kim(김동수),Dong-weon Lee(이동원) 대한기계학회 2021 대한기계학회 춘추학술대회 Vol.2021 No.6

In the recent years, the development of wireless sensors has attracted attention, with the development of MEMS(Micro-Electro-Mechanical-System) technology. The pressure sensor, one of the wireless sensor, is a device that measures the applied force, pressure, by a liquid or gas on a surface and translates it to the electrical signal. We fabricate this sensor based on capacitive type which measures change in capacitance with two metal plates. One of the two capacitor plates acts as the diaphragm that is displaced by the pressure, changing the capacitance of the circuit. Variation of signal detected by network analyzer evaluates the resonance frequency and phase shift with different interrogate distance and pressure force. [그림 본문 참조]

윤동주 「서시」의 의미 연구

김현수(Kim Hyeon-su) 한국시학회 2011 한국시학연구 Vol.- No.31

Yun, Dong-ju’s 「The First Poem」 is producing a variety of meanings by omitting its meanings and expressing it indirectly. This paper is to understand the meaning of the work, not the intention of the poet, based on the fact that literature is content to exist by itself. The meaning of a work is the real meaning which the work represents through linguistic combination and structure. This poem is divided into two stanzas. The first stanza of 1~8 lines is expressing the poet’s feelings, and the second stanza of 9 lines shows the imaginary scenes of nature. The first stanza is divided into 1~4 lines containing mental distress and 5~8 lines representing love and practice will. The scenes of the second stanza are symbolically representing the heart of the poet who has overcome conflicts and troubles. 「The First Poem」 is a work which has encapsulated the pure heart seeking beautiful and peaceful world with concise and implicative words. In terms of space, this poem is divided into Heaven and Earth, and in terms of conflicts, it shows the process of division and combination of the realistic ego which is tormented in the real world and the essential ego which wishes for the beautiful and peaceful heavenly world. Though the realistic ego is ambivalent and worried about the gap between reality and ideals, it recovers its essential ego through aiming for the heavenly world and pursues the peaceful and pure world which it always longs for inwardly.

고포도당이 백서 사구체 메산지움 배양세포에서 p38 MAPK 활성화 및 Fibronectin 생성에 미치는 영향

유태현 ( Yu Tae Hyeon ),허종호 ( Heo Jong Ho ),류동열 ( Lyu Dong Yeol ),김현욱 ( Kim Hyeon Ug ),이수현 ( Lee Su Hyeon ),김진주 ( Kim Jin Ju ),정동섭 ( Jeong Dong Seob ),최규헌 ( Choe Gyu Heon ),이호영 ( Lee Ho Yeong ),한대석 ( Han 대한신장학회 2003 Kidney Research and Clinical Practice Vol.22 No.5

목 적 : 고포도당으로 자극한 백서 메산지움 배양세포에서 p38 mitogen activated protein kinase (MAPK)의 활성화와 p38 MAPK의 상부와 하부인자로 알려져 있는 MAPK kinase 3/6 (MKK3/6)와 c-AMP responsive element binding protein (CREB)의 활성화, 그리고 fibronectin 합성을 자극 시간에 따라 관찰하고, p38 MAPK 경로와 fibronectin 합성 사이의 연관성을 규명함으로서 당뇨병성 신증의 병태생리를 규명하고자 하였다. 방 법 : 백서 메산지움 배양세포를 정상 포도당 (5.6 mM), 고포도당 (30 mM), 또는 정상 포도당+만니톨 (24.4 mM)로 자극한 후 첫째 p38 MAPK; 둘째 p38 MAPK의 상부 인자인 MKK3/6; 셋째 p38 MAPK의 하부인자인 CREB; 넷째 MAPK phosphatase-1 (MKP-1)의 변화를 자극 시간 (3분-48시간)에 따라 Western blot을 이용하여 관찰하였으며, 다섯째 fibronectin의 변화는 RT-PCR과 ELISA를 이용하여 확인하였다. 결 과 : 고포도당으로 자극한 백서 사구체 메산지움 배양세포에서 p38 MAPK 경로가 활성화 되었다. Total p38 MAPK 단백은 변화가 없었으나, 활성화된 phospho-p38 MAPK 단백은 고포도당군에서 자극 10분 후부터 정상 포도당군에 비해 의의있게 증가되었으며 (평균 1.9배), phospho-CREB 단백 역시 고포도당군에서 자극 10분 후부터 의미있게 증가되었다 (평균 2.5배). 이에 비해 p38 MAPK의 상부인자인 phospho-MKK3/6는 고포도당군에서 자극 3분 후부터 의의있게 증가되었다 (평균 2.4배). 또한, MKP-1 단백 발현은 고포도당군에서 p38 MAPK의 활성이 증가하기 시작한 시기와 동일한 시기 (자극 10분 후)부터 증가하기 시작하였다 (평균 1.9배). Fibronectin mRNA와 세포 배양액내 fibronectin은 고포도당군에서 자극 48시간 후에 각각 1.7배와 1.5배 증가되었으며, 이러한 증가는 p38 MAPK 억제제인 SB203580 (1 μM)에 의해 각각 73%와 69% 억제되었다. 결 론 : 고포도당으로 자극한 백서 사구체 메산지움 배양세포에서 p38 MAPK 경로의 활성화를 확인하였으며, p38 MAPK 억제제가 고포도당에 의한 fibronectin의 합성 증가를 억제시키는 것으로 보아 당뇨병성 신증에서 관찰되어지는 fibronectin의 축적에 p38 MAPK 경로가 관여할 것으로 생각된다. Background : The p38 mitogen-activated protein kinase (MAPK) pathway is activated by several stress factors, potentially leading to cellular apoptosis and growth. Little is known about the pattern of p38 MAPK pathway activation in mesangial cells under high glucose conditions. We examined the activity and expression of p38 MAPK members, 1x78 MAPK, MAPK kinase 3/6 (MKK3/6), c-AMP responsive element binding protein (CREE), and MAPK phosphatase-l (MKP-1) in cultured rnesangial cells exposed to high glucose. Methods : Mesangial cells were subcultured from rat glomeruli isolated by sieving technique. After serum restriction for 48 hours rnesangial cells were exposed to 5.6 mM glucose (low glucose, LG), 5.6 mM glucose+24.4 mM mannitol (LG+M), or 30 mM glucose (high glucose, HG) for 3 minutes to 48 hours with or without SU203580. Western blot was performed to determine the activity and protein expression of p38 MAPK members. R`I-I`CR and ELISA were performed for fibronectin mRNA expression and fibronectin synthesis, respectively. Results : p38 MAPK and CREB activities were significantly increased in rnesangial cells exposed to FIG compared with LG or LG+M after 10 minutes and was sustained at higher levels to 48 hours (p<0.05), but total p38 MAPK and CREB protein expressions did not differ. MKP--1 showed a similar pattern as p 3 MAPK and CREE (p<O.O.i). MKK3/6 acitvity was significantly higher in HG cells after 3 minutes and remained at higher levels throught the study period (p<0.05). Fibronectin mRNA expression and fibronectin synthesis were significantly increased in mesangial cells exposed to HG after 48 hours (p<0.05). SB203580 (1 pM) pretreatment for 1 hour significantly reduced HG-induced fibronectin mRNA expression and fibronectin synthesis by 73% and 69%, respectively (p<0.05). Conclusion : p38 MAPK activity was increased in mesangial cells exposed to HG in parallel with increased MKK3/6 activity, resulting in CREB activation and increased fibronectin synthesis. This activated p38 MAPK may play a role in the pathogenesis of diabetic nephropathy.

산업단지 및 일반생활 지역의 공기 중 톨루엔에 대한 개인노출 및 생체시료의 상관성 분석

이병준(Byoungjun Lee),허 정(Jung Heo),정다영(Dayoung Jung),김순신(Sunshin Kim),류현수(Hyeon-Su Ryu),최민지(Min-Ji Choi),솨이지엔페이(Jian-Fei Shuai),임성국(Sung-Guk Im),양원호(Wonho Yang) 한국환경보건학회 2017 한국환경보건학회지 Vol.43 No.4

Objectives: The purpose of this study was to assess the correlation relationship between personal exposure and urinary hippuric acid in biological monitoring for airborne toluene in an industrial complex and in general environments. Methods: Personal exposure to toluene and its metabolite, hippuric acid, in urine were simultaneously measured in occupational environments area near an industrial complex and in general environments. The study subjects were divided into three types: 137 workers who use organic solvents in the workplace, 210 residents living near a dyeing industrial complex, and 379 residents living in general environments. The toluene exposures of workers and residents were measured by a passive sampler for four days. The urine of participants was sampled when the passive samplers of personal exposure were collected. Results: The toluene and hippuric acid concentrations of workers were the highest, followed by the concentrations of residents living near a dyeing industrial complex and residents living in general environments. The coefficient of correlation between the concentrations of toluene and hippuric acid among workers was 0.749 (p<0.01) in the workplace. On the contrary, correlations between the concentrations of toluene and hippuric acid among residents living near a dyeing industrial complex and residents living in general environments were all not significant. The relationship between the concentrations of hippuric acid and toluene in three types could be described by the exponential growth model. Conclusions: This study analyzed the relationships between toluene exposure and the concentrations of hippuric acid in urine in high, middle, and low exposure environments, and could be described by the exponential growth model.

RT-LAMP를 이용한 콩황화일반모자이크바이러스의 진단

배대현, 이영훈, 김봉섭, 윤영남, 강범규, 최만수, 구성철, 김현태, 윤홍태, 이수헌, 백인열 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2

Soybean yellow common mosaic virus (SYCMV) has been recently reported, it has been occurred a lot with Soybean mosaic virus (SMV) and Soybean yellow mottle mosaic virus (SYMMV) in soybean field. SYCMV belongs to genus of Sobemovirus and induced viral symptoms with yellowing, mottle and mosaic. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SYCML-F3/B3/FIP/BIP from coat protein gene sequence of SYCMV. After the reaction of RTLAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I. under daylight and UV light. Optimal reaction condition was at 63 for 60min and the primers of RTLAMP showed the specificity for only SYCMV tested in this study.

진행성 폐 종양 환자에서 종양 내 OK-432 주입술의 효과

정수현,김성빈,박진희,탁희상,옥철호,장태원,정만홍,천봉권 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.2

Background: Intratumoral injection of OK-432 produced significant antitumor effects. There are reports that OK-432 was injected directly into tumor tissue on gastrointestinal tracts and HCC tissue, producing decrease in serum alpha-fetoprotein level. We adopted this technique for treating locally advanced lung cancer. The object of this study is to evaluate the local therapeutic efficacy, side effects of injection for treating symptomatic mass. Methods: Twenty lung cancer patients (13 males and 7 females) underwent sonography guided OK-432 injection. We analyzed relationships between response of OK-432 and response of skin test, histology and age. Pain that patients feel was evaluated by VAS (Visual Analog scale). Results: A significant decrease of tumor size was observed. The mean pre-injection diameter of the lesions was 54.7±11.3mm, PR rate were 15.0% (3/20) and minimal change (50%<tumor size decreased.) patients were 35.0% (7/20). The VAS score before injecting OK-432 into the lesion was 5±3, this was decreased to 2.5±1.5 (p=0.04). Object response was significantly correlated with skin response, adenocarcinoma, and age. Conclusion: A significant decrease of tumor size and pain was observed. So OK-432 is a valuable treatment option for patients with symptomatic lesion from advanced lung cancer who are not amenable to further therapy.

동시출현단어분석을 통한 다문화 교육 연구의 구조 분석

김현수 이화여자대학교 교육과학연구소 2016 교육과학연구 Vol.47 No.2

The purpose of this study was to analyze a structure of multicultural education studies by using co-word analysis. Data were 4,695 keywords from 1,685 multicultural education studies. First, after refining, keywords that occurred more than 15 times were selected. Second, using the selected keywords, a co-occurrence matrix and cosine similarity matrix were made. Third, hierarchical cluster analysis and network analysis were conducted. Results of the study are as follows. First, 6 clusters were classified by area of multicultural education; Language education(Cluster1), Teacher education(Cluster2), Intercultural education and Education for international understanding(Cluster3), Lifelong education(Cluster4), Civic education(Cluster5), Literature education(Cluster6). Second, 6 clusters were classified by ecological system in 3 factors; 1)Microsystem includes Cluster1(Multicultural families), Cluster2(Teacher), Cluster6(Literacy and Literature education), 2)Exosystem includes Cluster3(Multicultural society and policy), 3)Macrosystem includes Cluster4(Multiculture), Cluster5(Ism). Third, there were 4 carrier keywords between clusters; 1)Literature education (Cluster6) and Korean language education(Cluster1). 2)Bilingual education(Cluster1) and Multicultural society(Cluster3), 3)Multicultural society(Cluster3) and Social integration(Cluster5). Based on the results, the character of each cluster and recommendations for multicultural education are discussed.

다문화와 일반 가정의 어머니의 양육태도 및 아동의 감각처리능력 비교

함보현,김수경 대한감각통합치료학회 2010 대한감각통합치료학회지 Vol.8 No.1

목적 : 본 연구는 다문화와 일반 가정 어머니의 양육태도와 그 자녀의 감각처리능력을 알아보기 위한 것이다. 연구 방법 : 만 3~7세의 자녀를 둔 다문화 가정 19명과 일반 가정 38명을 조사하였다. 설문지는 어머니와 자녀의 일반적인 특성, 어머니의 양육태도, 자녀의 감각처리능력에 관한 것이다. 대상자의 인구학적 특성은 기술통계, 두 집단 간의 비교는 맨 휘트니 U 방법으로 분석하였다. 결과 : 다문화 가정과 일반 가정의 양육태도는 거부적 태도(p=.000)와 자율적 태도(p=.000)에서 유의한 차이를 나타내었다. 그리고 다문화 가정과 일반 가정 아동의 감각처리능력을 비교한 결과, 맛/냄새 민감성(p=.004), 움직임 민감성(p=.021), 과소반응/특정자극을 찾는 행동(p=.010), 청각여과하기(p=.016), 활력이 부족하고 허약함(p=.003), 시각/청각 민감성(p=.029), 총합(p=.002) 항목에서 유의한 차이를 나타내었다. 결론 : 본 연구를 통하여 다문화 가정과 일반 가정의 어머니 양육태도 특성과 만 3~7세 아동의 감각처리능력에 대해 알아보았다. 다문화 가정에 대한 심층적이고 질적인 연구를 통해 특성을 파악하고 놀이와 발달에 대한 정보를 제공하는 것이 필요하다. Objective : The purpose of the study is to investigate and compare multi-cultural families in terms of mother's attitude on child-rearing and their children’s sensory processing ability. Methods : 19 multi-cultural families and 38 ordinary families were investigated and all the targeted families had children aged 3 to 7 years. Data were collected by a questionnaire asking general characteristics of mother-child; mother’s attitude on rearing; and child’s sensory processing ability, the Korean-translated version of Short Sensory Profile [SSP], and the Mother nurturing attitude. Data analysis has been done by Descriptive Statistics for subject characteristics and by Mean-Whitney U test for comparison between multi-cultural family group and ordinary family group. Results : There are significant differences between the two family groups in terms of the rearing attitude, especially in sub-item ‘refusing '(p=.000)’ and ‘autonomous(p=.000)’. Result of the SSP also indicate significant differences between the two groups in total score(p=.002) and sub-items such as taste/smell sensitivity(p=.004), movement sensitivity(p=.021), underresponsive/seeks sensation(p=.010), auditory filtering(p=.016), low energy/weak(p=.003), visual/auditory sensitivity(p=.029). Conclusion : The results of this study well describe differences between multi-cultural families and ordinary families in maternal child-rearing attitude and sensory processing ability of the children. It is suggested to collect data regarding adaptation of multi-cultural families and their child development through more profound qualitative studies in further.