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Sri Hendrastuti Hidayat,Emma Rahmayani 한국식물병리학회 2007 Plant Pathology Journal Vol.23 No.2
in English). Bulletin HPT 1:26-31.Sudiono, Hidayat, S. H., Suseno, R. and Sosromarsono, S. 2001.Molecular detection and host range study of tomato-infectingbegomovirus. Proc. Indo. Phytopthol. Soc. Seminar pp. 208-217.Sukamto, Kon, T., Hidayat, S. H., Ito, K., Hase, S., Takahashi, H.and Ikegami, M. 2005. Begomoviruses associated with leafcurl disease of tomato in Java, Indonesia. J. Phytopathol. 153:562-566.Sulandari, S., Suseno, R., Hidayat, S. H., Sosromarsono, S. andHarjosudarmo, J. 2005. Detection and host range study ofgeminivirus causing pepper yellow leaf curl disease (Abstractin English). Hayati 13:1-6.van Regenmortel, M. H. V., Fauquet, C. M., Bishop, D. H. L.,
Hidayat, Sri Hendrastuti,Rahmayani, Enuna The Korean Society of Plant Pathology 2007 Plant Pathology Journal Vol.23 No.2
Whitefly-transmitted geminiviruses (WTGs) are economically important pathogens causing serious damage on tomato and chilli pepper in Indonesia. Geminiviruses are readily transmitted by its insect vector, sweetpotato whitefly (Bemisia tabaci). However, greenhouse whitefly (Trialeurodes vaporariorum), another species of whitefly, is commonly found together with B. tabaci in the field. Incidence of yellow leaf curl disease in tomato and chilli pepper is probably correlated with the population of whitefly complex. It is becoming important to find the role of T. vaporariorum in the spread of the disease. Therefore, research is conducted to study the characteristic relationship between tomato leaf curl begomovirus (ToLCV) and two species of whitefly. The two species of whitefly, B. tabaci and T. vaporariorum, was capable to transmit ToLCV although it was evidenced that B. tabaci is more effective as insect vector of ToLCV in tomato and chilli pepper. A single B. tabaci was able to transmit ToLCV to tomato with a minimum acquisition and inoculation access period of 10 h. Transmission of ToLCV by T. vaporariorum required at least 10 insects per plant with a minimum acquisition and inoculation access period of 24 h. The transmission efficiency will increase with longer acquisition and inoculation access period of the insect and the higher number of insect per plant.
Use of Serological-Based Assay for the Detection of Pepper yellow leaf curl Indonesia virus
Sri Hendrastuti Hidayat,Dedek Haryadi,Endang Nurhayati 한국식물병리학회 2009 Plant Pathology Journal Vol.25 No.4
Diseases caused by Pepper yellow leaf curl virus infection is considered to be emerging plant diseases in Indonesia in the last five years. One key factor for disease management is the availability of accurate detection of the virus in plants. Polyclonal antibody for Pepper yellow leaf curl Indonesia virus-Bogor (PYLCIV-Bgr) was produced for detection of the virus using I-ELISA and DIBA methods. The antibody was able to detect PYLCIV-Bgr from infected plants up to dilution 1/16,384 and cross reaction was not observed with Cucumber mosaic virus (CMV), Tobacco mosaic virus (TMV), and Chilli veinal mottle virus (ChiVMV). Positive reaction was readily detected in membrane containing Begomovirus samples from Yogyakarta (Kaliurang and Kulonprogo) and West Java (Bogor and Segunung). Infection of PYLCIVBgr in chillipepper, tomato, and Ageratum conyzoides was also confirmed using polyclonal antibody for PYLCIV-Bgr in DIBA. Polyclonal antibody for PYLCIVBgr is suggested to be included in disease management approach due to its good detection level.
Use of Serological-Based Assay for the Detection of Pepper yellow leaf curl Indonesia virus
Hidayat, Sri Hendrastuti,Haryadi, Dedek,Nurhayati, Endang The Korean Society of Plant Pathology 2009 Plant Pathology Journal Vol.25 No.4
Diseases caused by Pepper yellow leaf curl virus infection is considered to be emerging plant diseases in Indonesia in the last five years. One key factor for disease management is the availability of accurate detection of the virus in plants. Polyclonal antibody for Pepper yellow leaf curl Indonesia virus-Bogor (PYLCIV-Bgr) was produced for detection of the virus using I-ELISA and DIBA methods. The antibody was able to detect PYLCIV-Bgr from infected plants up to dilution 1/16,384 and cross reaction was not observed with Cucumber mosaic virus (CMV), Tobacco mosaic virus (TMV), and Chilli veinal mottle virus (ChiVMV). Positive reaction was readily detected in membrane containing Begomovirus samples from Yogyakarta (Kaliurang and Kulonprogo) and West Java (Bogor and Segunung). Infection of PYLCIV-Bgr in chillipepper, tomato, and Ageratum conyzoides was also confirmed using polyclonal antibody for PYLCIV-Bgr in DIBA. Polyclonal antibody for PYLCIV-Bgr is suggested to be included in disease management approach due to its good detection level.
Noor, Aidawati,Sri, Hendrastuti Hidayat,Rusmilah, Suseno,Soemartono, Sosromarsono The Korean Society of Plant Pathology 2002 Plant Pathology Journal Vol.18 No.5
Bemisia tabaci Genn. is an important pest worldwide because of its ability to cause damage by direct feeding and its role as a vector of some viruses including geminiviruses. The first report of Tobacco leaf curl virus (TLCV), a Geminiviruses, in Indonesia was in 1932 when the virus was found infecting tobacco plants in Central Java. The characteristic symptoms of TLCV included upward curling of the leaf edge, vein thickening, and sometimes the occurrence of enation on the underside of the leaves. Basic studies were carried out to elucidate the characteristics of TLCV transmission by its vector, B. tabaci. A single whitefly was able to transmit the virus and the efficiency of transmission was increased when the number of adult whiteflies was increased up to 20 per plant. Inoculation access period of 1 h could cause transmission up to 20% and the optimum inoculation access period was 12 h. Acquisition access period of 30 minutes resulted in 70% transmission while 1(10% transmission occurred with a 24-h acqui-sition access period. The virus was proven to be persistently but not transovarially transmitted. Discrete fragments of 1.6 kb were observed when polymerase chain reaction method was applied to detect the virus in viruliferous nymphs and individual adults of B. tabaci, while no bands were obtained from non-viruliferous nymphs and adults.