The expression patterns of RANKL and OPG in murine tooth eruption

Hwang, Kyung-Mun,Kim, Eun-Jung,Kim, Hyun-Jung,,Kim, Young-Jin,Nam, Soon-Hyeun 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.2

치아의 맹출은 치아기 (dental organ)와 치조골의 세포와 연관된 매우 복잡한 과정이다. 우선 치아 맹출이 일어나기 전에 파골세포가 치낭으로 집결하게 된다. 이러한 치낭의 역할은 파골세포와 조골세포의 상호작용으로 이루어 지는 골개조와 밀접한 관련이 있는데, 이는 치아 맹출과 연관된 많은 유전자들이 치낭에서 발현되기 때문이다. RANKL는 TNF ligand family로써 조골세포에 존재하며 파골세포의 형성 및 전구세포로 부터의 활성화를 유도한다. 이러한 RANKL은 OPG에 의해 그 작용이 억제되며 RANKL와 OPG의 상대적인 비율이 파골세포의 형성에 영향을 미친다. 또한 Runx2유전자의 변이는 조골세포의 분화와 활성에 차질을 가져오고 결국 RANKL/OPG pathway를 통해 파골세포 형성에 영향을 줄 수 있다. 치아의 발육 및 맹출에 미치는 RANKL 및 OPG의 영향을 알아보고 Runx2와의 연관성을 알아보기 위해 in situ hybridization 방법으로 태생 1, 3, 5, 7, 9, 11일된 쥐의 하악 및 제1대구치를 사용하여 실험을 실시한 결과 RANKL, OPG, Runx2의 mRNA가 태생 1일부터 11일까지 치낭 및 치아주위조직에 특성있게 나타났다. 이중 태생 5일에서 9일 사이에 RANKL 및 Runx2는 치아의 교합면측과 하방 치조골 부위의 발현이 강하게 나타난 반면 OPG는 약한 발현을 보였다. 이는 또한 파골세포의 활성부위를 알아보기 위해 TRAP염색을 실시하여 태생 5일에서 9일 사이에 최대의 활성화를 나타낸 결과와 연광성 있게 나타났다. RANKL, OPG, Runx2의 특성있는 발현양상들을 종합해 볼 때, 치아 맹출은 치낭, 치아기, 치조골 사이의 상호 작용을 통해 이루어 지며, 이는 치낭이 치아 맹출에 있어서 매우 중요하다는 것을 의미한다. 또한, 이러한 유전자들 (RANKL, OPG, Runx2) 이 치아의 맹출에 중요한 역할을 하는 것으로 사료된다. Tooth eruption is a complex and tightly regulated process that involves cells of the tooth organ and the surrounding alveolus. Osteoclast precursors must be recruited into the dental follicle prior to the onset of eruption. This function of dental follicle may be regarded as the ability of bone remodeling characterized by the interaction of osteoclasts and osteoblasts. This is because tooth eruption is a localized event in which many of the genes required for eruption are expressed in the dental follicle. RANKL is a membrane-bound protein that is a member of the TNF ligand family, which is present on bone marrow stromal cells and osteoblasts, and induces osteoclast formation and activation from precursor cell. The biologic effect of RANKL is inhibited by OPG and, in bone, the relative ratio of RANKL and OPG modulates osteoclastogenesis. To evaluate the roles of RANKL and OPG in tooth eruption and the relations with the expression pattern of Runx2, in situ hybridization was performed with mandibles of mice at postnatal stage 1, 3, 5, 7, 9 and 11. mRNA of RANKL, OPG, and Runx2 are expressed in dental follicle and surrounding tissue from P1 to 11. To determine the sites of osteoclastic activity duing tooth eruption, mandibles were dissected. Peak osteoclastic activity in alveolar bone along the occlusal and basal regions was observed from P5 to 9, with osteoclasts in these regions being large and strongly TRAP-positive. The specific spatic-temporal expression patterns of RANKL, OPG and Runx2 in our study suggest that tooth eruption could be progressed through the interactions of molecular signaling among dental follicle, dental organ and alveolar bone, furthermore it means that dental follicle is quite important in tooth eruption. In addition, it indicates that these genes (RANKL, OPG, and Runx2) play critical roles in tooth eruption.

1차원 random media에서 Random walk에 관한 연구

黃甲淳,金慶植 釜山水産大學校 1987 釜山水産大學 硏究報告 Vol.27 No.1

The random walk can be described by the master equation in one-dimensional random media. We study both numerically and analytically the long time asymptotic behavior of the first moment, for the model systems of discrete-time and continuous-time random walks characterized by the transition probability density in the case of nearest neighbor transition. The results of our Monte Carlo method are also showed.

Polyozellus multiplex가 생산하는 지질과산화 저해물질

황지숙,송경식,김양섭,석순자,이태호,유익동 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.5

담자균류로부터 지질과산화 저해물질을 탐색하던 중 강원도 오대산 지역에 자생하는 버섯의 한 종류로 부터, Sparague-Dawley계 rat의 간 mirosome 분획의 지질과산화를 강하게 억제하는 물질이 있음을 발견하였다. 이 버섯의 미생물학적 특성을 관찰한 결과 민주름버섯목, 굴뚝버섯과에 속하는 까치버섯(Polyzellus multiplex)으로 동정하였으며 본 버섯의 자실체 methanol 추출물을 benzene, ethylacetate로 순차적으로 추출하고 각종 chromatography를 이용하여 활성물질을 정제한 결과, ethylacetate층으로 부터 황산화활성을 나타내는 PM1을 benzene층으로 부터 지방산 계열의 PM2와 PM3 화합물을 순수하게 분리 정제하였다. PM1은 정색반응 및 각종 기기분석 결과 thelephoric acid계의 화합물로 동정되었으며 PM2 및 PM3는 각각 linoleic acid와 oleic acid로 동정되었다. 지질과산화 활성물질로 분리정제한 PM1 화합물의 IC_50 값은 1.96 ppm이었으며 LD_50 값은 500 mg/Kg 이상이었다. In the course of screening lipid peroxidation inhibitor from basidiomycetes, a mushroom, which was collected at O-Dae mountain in Kangweon-Do, was found to have active compound. The mushroom was identified as Polyzellus multiplex, which belongs to Aphylloporalles Thelephoraceae, on the basis of macroscopic and microscopic characteristics of the fruiting body. The methanol extract of fruiting body was extracted with benzene and ethylacetate, sequentially. By using various kinds of chromatographies, PM1, and PM2 and PM3, were purified from the ethylacetate extract and the benzene extract, respectively. Color reaction and analyses of IR, UV, and NMR spectra indicated that PM1 was a derivative of thelephoric acid, and PM2 and PM3 were linoleic acid and oleic acid, respectively. IC_50 of PM1 for inhibition of lipid peroxidation was 1.96 ppm and LD_50 was 500 mg/kg.

OCT 플라스미드를 갖는 원유 분해세균에 의한 Octane 분해능

최순영,김창숙,이명혜,황문옥,민경희 숙명여자대학교 자연과학연구소 1991 자연과학논문집 Vol.- No.2

원유 분해세균에 의한 원유 분해능을 조사한 결과, Xanthomonas campestris M12. Xanthomonas sp. M28, Acinetobacter lwoffi G1, and Klebsiella pneumoniae L25, 그리고 Pseudomonas maltophilia N246 등의 순서로 나타났다. Xanthomonas campestris M12, Xanthomonas sp. M28, 그리고 Pseudomonas maltophilia N246 균주 모두 octane 분해시의 온도는 30℃에서 최적이었으며, 최적 pH는 X. campestris M12와 Xanthomonas sp. M28이 7.0∼7.5이었고, P. maltophilia N246이 7.5∼9.0이었다. N246 균주의 최적 NaCl 농도는 3.0∼3.5%이었다. P. maltophilia N246와 X. campestris M12는 모두 플라스미드를 갖고 있음을 확인하였고, N246 균주로부터 플라스미드를 제거하였을 경우 octane 분해능이 소실되었으므로 이 플라스미드 위에 octane 분해 유전자가 있음이 확인되었다. 이 균주의 OCT 플라스미드의 크기는 118kb이었다. 또한, N246 균주는 ampicillin 항생제에 내성을 나타내었다. Xanthomonas campestris M12. Xanthomonas sp. M28. Acinetobacter lwoffi G1, and Klebsiella pneumoniae L25, Pseudomonas maltophilia N246 were screened to increase the ability for crude oil utilization. All of these could utilize hexadecane and octane with the exception of N 246 strain for only octane biodegradation. Thus N246, M12, and M28, strains were specially examined for octane oxidation. Octane biodegradation by three strains showed the optimal conditions at 30C, pH7.0∼9.0 and 0.2∼0.3% octane concentration as a substrate. It was found that P. maltophila N246 and X. campestris M12 had plasmid and the cured plasmid from N246 strain lost octane utilization. Therefore, it was confirmed that certain genes for octane utilization were located on OCT plasmid in N246 strain. The size of OCT plasmid in N246 strain was 118 kb. The N246 strain was resistant to ampicillin.

간호중재분류체계(NIC)를 이용한 중환자실 간호중재 분석

권경남,장희정,황운순,김은주,이미경 영남이공대학 산업기술연구소 2000 産業技術硏究 Vol.12 No.-

The purpose of this research was to identify nursing interventions performed by the ICU nurses from 2 university hospital in Taegu. For this research, I used the Nursing Intervention questionnaire developed by the Iowa Intervention Project and translated by korean specialists and professor concerned to the same field. In order to obtain the data, a special group of professor, head nurse, charge nurse, researcher was instituted. The special group work validataed 205 nursing interventions with agreement of more than 50% becuse the NIC(433 nursing intervention) survey process was too time-consuming and not appropriate for all labels. The data were collected from June 1 to 30, 1999 and analysed with SAS program. The obtained results are as follows; (1) 94 interventions were performed at least daily. (2) The most frequently used nursing interventions were Parenteral Medication Administration, Aspiration Precautions, Pressure Ulcer Prevents, Positioning, Airway Management, Cough Enhancement in rank. In conclusion, this research using NIC has helped to figure out the component to the ICU care in korea. The use of this results will contribute to enhance quality assessment and improve standard of ICU nursing.

안면비대칭에 대한 주관적 인지도와 정모두부방사선사진 분석치의 연관성

안정순,황현식,김창수,김경근 전남대학교 치의학연구소 2001 구강과학 Vol.13 No.3

This study was aimed how posteroanterior cephalometric measurements affect the perception of the patient about his/her facial asymmetry. One hundred orthodontic patients, over the age of 12, who required a posteroanterior cephalogram in the Department of Orthodontics of Chonnam National University Hospital were used as the subject. They were asked if they thought their faces are asymmetrical. Their responses were classified into 5 groups based on the level of asymmetry as follows : Definitely No, Probably No, Don't Know. Probably Yes, Definitely Yes. Nine linear and four angular measurements from each posteroanterior cephalometric radiographs were analysed on the standard of the line between crista galli and anterior nasal spine to show the extent of asymmetry. Through this comparative study, the following results were obtained. 1. As the deviation of menton and the midline discrepancy of the upper and lower jaws were greater, the perception of patients about their facial asymmetry was higher. 2. All the measurements from the group "Don't Know" showed no statistical difference with those of the groups "Definitely No" or "Probably No." 3. All the measurements from the group "Probably Yes" showed no statistical difference with those of the group "Definitely Yes." The above results suggest that it may be helpful for the orthodontic diagnosis to ask patients' perception of their own facial asymmetry.

품종별 쌀의 이화학적 특성과 증편제조 적성과의 관계

우경자,이은아,황홍구,이건순 동아시아식생활학회 1998 동아시아식생활학회지 Vol.8 No.4

This study was carried out in order to investigate the processing adaptability of Jeung-pyun. We used rice materals that were developed domestically for improving the utilization of rice. Six cultivars(Ilpoombyeo, Hwasungbyeo, Dasanbyeo, Daeribbyeo 1, Hyangmibyeo 1, Hyangmibyeo 2) of rice were used for making Jeung-pyun. In physicochemical properties of rice, Hwasungbyeo had the highest amylose content(20.4%) and Hyangmibyeo 2 had the lowest one(14.9%). Lipid content was 0.23%(Daeribbyeo 1)~0.43%(Ilpoombyeo), protein content was 7.94%(Ilpoombyeo)~8.43%(Dasanbyeo), and ash content was 0.15%(Hyangmibyeo 2)~1.24%(Daeribbyeo 1). In volume and specific volume of the 3 hour fermented Jeung-pyuns, Ilpoombyeo, Hwasungbyeo, Hyangmibyeo 2 were higher, but in the 7 hour fermented Jeung-pyuns, Dasanbyeo, Daeribbyeo 1, Hyangmibyeo 1 were higher. In the sensory evaluation of the test Jeung-pyuns, the overall quality of the 3 hour fermented Jeung-pyuns was not significantly different among the rice cultivars, whereas in the 7 hour fermented Jeung-pyuns Hyangmibyeo 2 had the lowest values. When the 7 hour fermented Jeung-pyuns were stored at 20℃, the hardness and brittleness of Jeung-pyuns were respectively increased, the cohesiveness was respectively decreased. The elasticity was not significantly different among the rice cultivars with storage days, and the gumminess was significantly difference between rice cultivars, respectively increasing during storage, but Hyangmibyeo 2 was the lowest, not increased. In instrumental characteristics of Jeung-pyuns, the hardness was correlated with amylose content and the brittleness and gumminess were highly correlated with the hardness.

RISC 구조 16-bit fixed-poing DSP core의 기능 블럭 설계

洪淳錫,黃義暻 홍익대학교 산업기술연구소 1999 産業技術 Vol.9 No.-

Digital signal processing mainly needs high speed arithmetic operations in real time. Therefore in order to process signals at high speed, the DSP with special hardware structures including multiplier, barrel shifter, and junction table etc. has appeared. In this paper, we designed function block of 16-bit fixed-point DSP core with RISC structure. Internal structure of DSP core is designed with each function block. Multiplier, ALU, Barrel Shifter of data part and Register part are programed in VHDL. Especially, designed adder which is one of multiplier's composition elements showed more progressive result than conventional CMOS adder and CPL adder about delay time and poser consumption. Timing diagram of MAC struction for design of control signal block is manufactured and implemented with ASM(Algorithmic State Machine), ASM chart is synthesized logically and then state-machine of MAC instruction is based on the results obtained

지역적 특성을 고려한 보육시설모형에 관한 연구

최경순,황연숙,정지영 신라대학교 자연과학연구소 1999 自然科學論文集 Vol.6 No.-

This study aims to grasp the present condition of nurseries, to investigate and analyse case studies, to suggest standards and reform measures, and based on these, to produce basic information for the formation of an architectural spatial model. Data were collected through questionnaire survey including observations and interviews. The sample consisted of 82 Child Care Centers. The data were analized by using SPSS-PC program. The major findings were as follows : (1) The facility and equipments in the child care centers were insufficient for children's development. (2) Most of child care centers are not in the desirable Interior environment. To enhance the quality of child care centers, the physical interior environment of child care centers should be influence on the development of child. (3) Functional spaces should be organized to support the child' behavior. Multi-purposed room should be provide to encourage and adapt to variety of large-muscle activities. (4) Interior findings looks too monotonous and do not have characteristics. Natural materials, various colors and patterns are recommendable. Furniture should be designed to meet the functions of flexibility, mobility, and security.

조대술에 의한 하마종의 치료

강동균,황경문,김은정,김현정,김영진,남순현 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.1

하마종은 구강저에 발생하는 점액 남종으로 주로 설하선과 관련된다. 즉, 하마종은 설하선 분비도관의 파열로 인하여 타액이 분비도관에서 빠져나와 주위의 연조직 내에 저류됨으로써 발생하는 가성 낭으로 정의 되며, 원인은 설하선 도관의 파열이나 폐색이다. 하마종의 임상소견은 청색의 투명한 종창이 구강저의 소대를 중심으로 편측으로 발생하며, 무통성이며, 촉진시 파동성을 나타낸다. 조직소견은 결합 조직 내 와동이 형성되지만, 낭 벽은 상피세포가 아닌 육아조직으로 구성되는 가성 낭을 관찰할 수 있다. 하마종의 첫 번째 치료법으로는 하마종의 내벽이 구강점막의 일부가 되도록 만들어 주는 조대술을 고려할 수 있다. 본 증례는 특징적인 하마종의 임상소견을 보이는 환아에게 gauze-packing등을 동반한 조대술을 이용하여 치료한 증례로써 양호한 치료결과를 보여 보고하고자 한다. Ranula is a mucocele which occurs at the mouth floor and mostly related to sublingual gland. In other words ranula is definded as a pseudocyst which occurs as the secretion duct of sublingual gland is destructed there as the saliva from the the secretion duct flows out and retention in the soft tissue. The cause of ranula is destruction or obstruction of the duct. The clinical findings of ranula is a painless, unilateral bluish transparent swelling around the frenum and shows fluctuation when palpated. Histological finding represent a formation of cavity inside the connective tissue. but a pseudo-cyst can be seen which the wall of the cyst is composed of granulation tissue rather than epithelial cells. The first treatment of ranula can be considered as marsupialization. which induces the inner wall of the Ranula to be a part of oral mucosa. This case report shows a treatment of marsupialization with gauze packing in a young patient representing a clinical finding of characteristic ranula.